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#34129163   2021/06/15 To Up

Evaluation of sex steroid hormones and reproductive irregularities in diethyl phthalate-exposed premature mice: modulatory effect of raw honey against potential anomalies.

Phthalates, plasticizing chemicals, are top-rated environmental contaminants. Diethyl phthalate (DEP), a chief member of this family, was declared a potent endocrine disruptor and carcinogen in animals and humans. The current study was designed to explore the probable reproductive damage induced by DEP and the therapeutic efficacy of raw honey in male albino mice. Four-week-old 50 male mice were randomized equally in five groups, as control (C) received 0.1 ml distilled water; vehicle control (VC) received corn oil (0.1 ml/mouse); DEP (3mg/g/BW) dissolved in corn oil; honey control (HC) administered with honey (0.2 mg/g/day); and phthalate plus honey (P+H) administered with DEP and honey (3mg and 0.2 mg/g/BW/day respectively). Mice were treated through oral gavage for 54 days routinely, acclimatized for 6 days, and dissected. In the first instance, the antioxidant potential and total phenolic contents (TPC) of honey were analyzed through ferric reducing antioxidant power (FRAP) assay and Folin-Ciocalteu assay to confirm the antioxidant capacity of honey. The morphological, morphometric, histological, micrometric, sperm count, and hormonal analyses, and antioxidant capacity test in tissue homogenates were conducted by using tissues (testis, epididymis) and blood samples of mice. Mice exposed to DEP have a significant increase in body weight, LH level, and seminiferous tubule lumen diameter and decrease in the gonado-somatic index, testosterone level, sperm count, and seminiferous tubule diameter. Additionally, histopathology of testes showed interstitial space dilations, exfoliations, Leydig cell atrophy, germ cell degenerations, and spermatid retention in DEP-exposed testes sections. However, concomitant use of honey and DEP had shown a significant improvement in histopathological lesions, steroid hormone levels, and healthy sperm count. By these results, it is concluded that honey possessed antioxidant potential that can efficiently protect DEP-induced anomalies in male mice.
Chaman Ara, Asmatullah, Faiza Yaseen, Shaukat Ali, Hafiz Abdullah Shakir, Muhammad Khan, Shagufta Andleeb, Nageena Ramzan

1796 related Products with: Evaluation of sex steroid hormones and reproductive irregularities in diethyl phthalate-exposed premature mice: modulatory effect of raw honey against potential anomalies.

5 G250 mg50 mg96 wells (1 kit)2ug2ug100 μg

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#34125507   2021/06/14 To Up

Analysis of Polycyclic Aromatic Hydrocarbons and Phthalate Esters in Soil and Food Grains from the Balkan Peninsula: Implication on DNA Adduct Formation by Aristolochic Acid I and Balkan Endemic Nephropathy.

Balkan endemic nephropathy (BEN) is a chronic tubulointerstitial nephropathy affecting residents of rural farming areas in many Balkan countries. Although it is generally believed that BEN is an environmental disease caused by multiple geochemical factors with much attention on aristolochic acids (AAs), its etiology remains controversial. In this study, we tested the hypothesis that environmental contamination and subsequent food contamination by polycyclic aromatic hydrocarbons (PAHs) and phthalate esters are AA toxicity factors and important to BEN development. We identified significantly higher concentrations of phenanthrene, anthracene, diethyl phthalate (DEP), dibutyl phthalate (DBP), and benzyl butyl phthalate (BBP) in both maize and wheat grain samples collected from endemic villages than from nonendemic villages. Other PAHs and phthalate esters were also detected at higher concentrations in the soil samples from endemic villages. Subsequent genotoxicity testing of cultured human kidney cells showed an alarming phenomenon that phenanthrene, DEP, BBP, and DBP can interact synergistically with AAs to form elevated levels of AA-DNA adducts, which are associated with both the nephrotoxicity and carcinogenicity of AAs, further increasing their disease risks. This study provides direct evidence that prolonged coexposure to these environmental contaminants via dietary intake may lead to greater toxicity and accelerated development of BEN.
Wanlin Guo, Jiayin Zhang, Zhihan Sun, William H Orem, Calin A Tatu, Niko S Radulović, Dragan Milovanović, Nikola M Pavlović, Wan Chan

1169 related Products with: Analysis of Polycyclic Aromatic Hydrocarbons and Phthalate Esters in Soil and Food Grains from the Balkan Peninsula: Implication on DNA Adduct Formation by Aristolochic Acid I and Balkan Endemic Nephropathy.

10 mg2.5 mg100ug Lyophilized1,000 tests100ug5mg25 g1 mg100ug100ug Lyophilized

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#34118542   2021/06/02 To Up

Occurrence and migration of phthalates in adhesive materials to fruits and vegetables.

Labels or tapes are widely used on fresh fruits and vegetables, which may contain phthalates (PAEs). There are few studies on the contamination pathway of PAEs from labels or tapes to food stuff. In this study, the concentrations of eleven PAEs in adhesive labels, tapes, labeled fruits and vegetables on the market were investigated. The eleven PAEs were detected with the total concentration of 7.44-30.51 mg/m in labels and tapes. Diethyl phthalate (DEP), di-n-butyl phthalate (DIBP), dimethyl phthalate (DMP), Bis (2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) had the highest detection frequency in adhesive material samples. The concentrations of PAEs in labeled fruits were higher than that in unlabeled fruits, especially in the peel, indicating PAEs could transfer from labels or tapes to fruits and vegetables. Furthermore, the migration behaviors of PAEs from labels or tapes to apples, avocados and celery were investigated. It was found that the PAEs could penetrate to apple and avocado pulp through the peel, resulting in the residue of the PAEs in the whole fruit. Unlike apple peel, the thick avocado peel was more difficultly penetrated by the PAEs. Due to the high lipid content, the PAEs distributed more evenly in avocado pulp than in apple pulp. The migrations up to a maximum of 4.16 mg/kg were found for butyl benzyl phthalate (BBP) in avocado peel and up to a maximum of 0.188 mg/kg in avocado pulp. The average migration of the PAEs in celery ranged from 0.3 to 26.1 μg/kg in three days and the low migration might result from the rough surface and less contacting area. These findings suggest that the use of labels or tapes in direct contact may increase the risk of PAEs exposure to humans through fruits and vegetables.
Haonan Hou, Yihao Min, Xueke Liu, Peng Wang, Zhiqiang Zhou, Donghui Liu

2648 related Products with: Occurrence and migration of phthalates in adhesive materials to fruits and vegetables.

10 mg100 mg5ug96 wells (1 kit)100ug1 ml200ul100 mg 25 MG100ul25 mg

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#34115461   2021/06/11 To Up

Strong Periodic Tendency of Trivalent Lanthanides Coordinated with a Phenanthroline-Based Ligand: Cascade Countercurrent Extraction, Spectroscopy, and Crystallography.

Phenanthroline-diamide ligands have been reported in the selective separation of actinides over Eu(III); on the contrary, relevant basic coordination chemistry studies are still limited, and extraction under actual application conditions is rarely involved. In this work, -diethyl--ditolyl-2,9-diamide-1,10-phenanthroline [Et-Tol-DAPhen ()] was applied to explore the coordination performance of lanthanides in simulative high-level liquid waste. For the first time, cascade countercurrent extraction was conducted with Et-Tol-DAPhen as the extractant, which reveals the periodic tendency of the extraction efficiency of lanthanides to decrease gradually as the atomic number increases. Comparison of elements with similar radii verifies the hypothesis that the increase in the atomic number leads to a decrease in the ionic radius, thus reducing the coordination and extraction capacity of ligands. Slope analysis, electrospray ionization mass spectrometry, and ultraviolet-visible titration results show that the ligand forms 1:1 and 1:2 complexes with lanthanides and the coordination ability follows the tendency of extraction efficiency, and the first crystal structures of Lns(III) with a phenanthroline-diamide ligand, i.e., [La(NO)(HO)] and [La(NO)][(NO)], were obtained, which confirms the conclusions described above. This work promises to enhance our comprehension of the chemical properties of Lns(III) and offer new clues for the design and synthesis of novel separation ligands.
Xiao-Fan Yang, Peng Ren, Qi Yang, Jun-Shan Geng, Jin-Yu Zhang, Li-Yong Yuan, Hong-Bin Tang, Zhi-Fang Chai, Wei-Qun Shi

2353 related Products with: Strong Periodic Tendency of Trivalent Lanthanides Coordinated with a Phenanthroline-Based Ligand: Cascade Countercurrent Extraction, Spectroscopy, and Crystallography.

Proteinase K Powder, 2 ea500 MG 250 ml 50 25 mg1000 100 extractions10 mg100ul100ug50 mg 1 kit(s)

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#34114261   2021/06/10 To Up

Inclusion Complexes of Gold(I)-Dithiocarbamates with ß-Cyclodextrin: A Journey from Drug Repurposing towards Drug Discovery.

We identified the gold(I)-dithiocarbamate (dtc) complex [Au(N,N-diethyl)dtc]2 as the active cytotoxic agent in the combination treatment of sodium aurothiomalate and disulfiram on a panel of cancer cell lines. In addition to demonstrating pronounced differential cytotoxicity to these cell lines, the gold complex showed no cross-resistance in therapy-surviving cancer cells. In the course of a medicinal chemistry campaign on this class of poorly soluble gold(I)-dtc complexes, we synthesized >35 derivatives and used x-ray crystallography to examine structural aspects of the dtc moiety. A group of hydroxy-substituted complexes has an improved solubility profile, and we found that these complexes form 2:1 host-guest inclusion complexes with β-cyclodextrin (CD), exhibiting a rarely observed "tail-to-tail" arrangement of the CD cones. Formulation of a hydroxy-substituted gold(I)-dtc complex with excess sulfobutylether-β-CD prevents the induction of mitochondrial reactive oxygen species, which is a major burden in the development of metallodrugs.
Michael Morgen, Piotr Fabrowski, Eberhard Amtmann, Nikolas Gunkel, Aubry Kern Miller

2010 related Products with: Inclusion Complexes of Gold(I)-Dithiocarbamates with ß-Cyclodextrin: A Journey from Drug Repurposing towards Drug Discovery.

100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays

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#34112423   2021/05/19 To Up

Aroma characteristics of traditional Huangjiu produced around Winter Solstice revealed by sensory evaluation, gas chromatography-mass spectrometry and gas chromatography-ion mobility spectrometry.

Traditional Huangjiu (a kind of traditional Chinese rice wine) produced around Winter Solstice has higher quality and a more harmonious aroma than those produced during other periods. To determine the specific differences in aroma characteristics, sensory evaluation, gas chromatography-mass spectrometry (GC-MS) and gas chromatography-ion mobility spectrometry (GC-IMS) were used to analyze the volatile profiles of the traditional Huangjiu samples produced under different ambient temperature conditions. The sensory evaluation results showed that the aroma attributes of wheat, sweet, ester, alcoholic and sauce were stronger for the samples fermented near Winter Solstice than those for the other samples. GC-MS combined with heatmap analysis showed that with the decrease in average ambient temperature, the contents of esters such as diethyl succinate and ethyl butanoate gradually increased, and the contents of alcohols such as phenylethyl alcohol, 2-methylpropanol and 3-methylbutanol gradually decreased. Some key aroma compounds, such as ethyl butyrate (OAV: 97-151), nonanal (OAV: 189-200), ethyl octanoate (OAV: 859-1134) and ethyl phenylacetate (OAV: 307-353), were more abundant in the samples fermented near Winter Solstice than the other samples. The visualization of GC-IMS suggested that isoamyl acetate, 2-methylpropyl acetate, ethyl 3-methylbutyrate, and ethyl 2-methylbutanoate were enriched near Winter Solstice. Together, the results suggested that the traditional Huangjiu produced around Winter Solstice contained more flavor volatiles and had better aroma quality than those produced during other periods.
Haiyan Yu, Wei Guo, Tong Xie, Lianzhong Ai, Huaixiang Tian, Chen Chen

2660 related Products with: Aroma characteristics of traditional Huangjiu produced around Winter Solstice revealed by sensory evaluation, gas chromatography-mass spectrometry and gas chromatography-ion mobility spectrometry.

100ug LyophilizedOne 96-Well Strip Micropl96T100ug 100ul100ug Lyophilized1000 100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#34111848   2021/06/10 To Up

Tip-Induced β-Hydrogen Dissociation in an Alkyl Group Bound on Si(001).

Atomic-scale chemical modification of surface-adsorbed ethyl groups on Si(001) was induced and studied by means of scanning tunneling microscopy. Tunneling at sample bias > +1.5 V leads to tip-induced C-H cleavage of a β-hydrogen of the covalently bound ethyl configuration. The reaction is characterized by the formation of an additional Si-H and a Si-C bond. The reaction probability shows a linear dependence on the tunneling current at 300 K; the reaction is largely suppressed at 50 K. The observed tip-induced surface reaction at room temperature is thus attributed to a one-electron excitation in combination with thermal activation.
Alexa Adamkiewicz, Tamam Bohamud, Marcel Reutzel, Ulrich Höfer, Michael Dürr

1240 related Products with: Tip-Induced β-Hydrogen Dissociation in an Alkyl Group Bound on Si(001).

100ug100 ul 100ul100ug100 ul50 ul100ug50 ul1 ml500 100 μg15

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#34111639   2021/06/03 To Up

Critical analysis of peptidoglycan structure of Lactobacillus acidophilus for phthalate removal.

Our previous studies have shown that lactic acid bacteria (LABs) can bind and remove di-n-butyl phthalate (DBP), diethyl phthalate, and dioctyl phthalate; three ubiquitous environmental phthalate contaminants. In this study, Lactobacillus acidophilus NCFM was chosen to study the DBP binding mechanism. We found that the three-dimensional structure of the bacterial cell wall, including the carbohydrates and proteins, was essential for DBP adsorption. Peptidoglycan was the main binding component in the cell wall (80.71%), and binding sites exposed to DBP were C-N, N-H, O-H, and C-O bonds. Molecular dynamic (MD) studies demonstrated that hydrophobic interaction plays an important role in DBP adsorption, the chemical sites that influenced the binding in the peptidoglycan model were O2, O3>N1, N2, N3>O1, O4, and the form of adsorption force included hydrogen bonding force, electrostatic force, and van der Waals forces. These theoretical data from the MD simulation were consistent with the experimental results in terms of the ability of this bacterium to bind DBP, so the MD simulation proposed a new way to investigate the mechanisms of phthalate binding to LABs.
Lili Zhao, Junyan Wei, Xin Pan, Yu Jie, Baoqing Zhu, Hongfei Zhao, Bolin Zhang

1591 related Products with: Critical analysis of peptidoglycan structure of Lactobacillus acidophilus for phthalate removal.

5 G100 tests1 module 1 G 15 ml 1L1 module0.1 mg500 MG1 module25 µg

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#34104051   2021/04/28 To Up

Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties.

In the present work, essential oil and fatty acids and extracts obtained from aerial parts of  Boiss. & Bal. were investigated for chemical composition and biological activities. The phytochemical analyses were conducted with gas chromatography-mass spectrometry/flame ionisation detector (GC-MS/FID) and liquid chromatography-mass spectromtetry (LC-MS/MS) techniques. The extracts and essential oil were studied for -amylase and acetylcholinesterase activities with two different spectrophotometric methods. Antimicrobial activities of the extracts were investigated by microdilution. The extracts were evaluated  for cytotoxic effects against cancer and normal cell lines by MTT assay. The essential oil (EO) contained -pinene (12.5%) and -caryophyllene (10.7%) as main compounds. Palmitic(26.5%) and nonadecanoic acids (26.6%) were determined as fatty acids. Phytochemical analysis of the extracts found phenolic acids, phlinosides, verbascoside, and flavonoids. The extracts and essential oil demonstrated poor -amylase inhibitory activity. The best acetylcholinesterase inhibitory activity was obtained for diethly ether extract of (67.2 ± 3.4%) at 10 mg /mL concentration. Ethyl acetate extract found to be effective against at a minimum inhibitory concentration (MIC) of 156.26 µg/mL. Diethyl ether extract of was active on A549 cell lines with an IC = 316 ± 4.16 µg/mL when compared with cisplatin IC = 24.43 ± 0.14 µg/mL. To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-α-amylase, and antimicrobial activities, as well as cytotoxic effects of .
Gamze Göger, Ümmühan Türkyolu, Ezgi Nur Gürşen, Süleyman Yur, Abdullah Burak Karaduman, Fatih Göger, Mehmet Tekin, Gülmira Özek

2725 related Products with: Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties.

100 mg10 mg10 mg1 ml1000 1,000 tests200ug1 mg25 mg1000 tests100ug

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#34101501   2021/06/08 To Up

Preparation of Rhenium-188-Lipiodol Using Freeze-Dried Kits for Transarterial Radioembolization: An Overview and Experience in a Hospital Radiopharmacy.

Rhenium-188-lipiodol is a clinically effective, economically viable radiopharmaceutical for Selective Internal Radiation Therapy of liver cancer. Present study evaluates the performance of three freeze-dried kits with respect to the radiochemistry, quality control, and overall "ease of preparation" aspects in a hospital radiopharmacy. Freeze-dried kits of acetylated 4-hexadecyl-4,7-diaza-1,10-decanedithiol (AHDD), super six sulfur (SSS), and diethyl dithiocarbamate (DEDC), obtained commercially or received as gift, were used for the preparation of Rhenium-188-lipiodol using freshly eluted Re-sodium perrhenate from commercial Tungsten-188/Rhenium-188 generator following recommended procedures. The overall yield of Rhenium-188-lipiodol prepared using AHDD Kit, SSS Kit, and DEDC Kit was 74.82% ± 3.3%, 87.55% ± 4.8%, and 76.38% ± 4.6%, respectively. Observed radiochemical purity (RCP) of Rhenium-188-lipiodol prepared using these kits was 88.65% ± 2.8%, 92.92% ± 3.0%, and 91.38% ± 3.0%, respectively. Using a modified version of the DEDC Kits, overall yield of 87.17% ± 2.7% and RCP of 95.43% ± 2.3% could be achieved. While all three freeze-dried kits can be used for the preparation of Rhenium-188-lipiodol in >70% overall yield, the modified version of DEDC Kits has some advantages in terms of preparation time and volume of Rhenium-188 activity that can be added to the kit vial. The latter feature of the DEDC Kit is particularly useful for patient dose preparation with Rhenium-188 activity of low radioactive concentration.
Edathurutykalarickal Ramakurup Radhakrishnan, Viju Chirayil, Arun Pandiyan, Suresh Subramanian, Madhava B Mallia, Koramadai Karuppusamy Kamaleshwaran, Ajit Shinto

2252 related Products with: Preparation of Rhenium-188-Lipiodol Using Freeze-Dried Kits for Transarterial Radioembolization: An Overview and Experience in a Hospital Radiopharmacy.

100 μg100ug Lyophilized100 μg100 μg100ug100 μg100ug Lyophilized1 Set1 Set100ug Lyophilized1 Set

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