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#33084207   2020/10/21 To Up

Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins.

Giant unilamellar lipid vesicles (GUVs) are widely used as model membrane systems and provide an excellent basis to construct artificial cells. To construct more sophisticated artificial cells, proteins-in particular membrane proteins-need to be incorporated in GUVs. However, current methods for protein reconstitution have limited throughput or are not generally applicable for all proteins because they depend on detergent solubilization. This limitation is addressed here by introducing calcium-mediated membrane fusion to transfer proteins between negatively charged GUVs and cell-derived plasma membrane vesicles (CDVs), derived from HEK293T cells overexpressing a membrane receptor protein. Fusion conditions are optimized using large unilamellar vesicles and GUVs containing phosphatidylserines and fusogenic lipids. The approach is then applied to induce lipid mixing and subsequent transfer of the overexpressed membrane receptor from CDVs into GUVs. The membrane receptor is detected by immunofluorescence on GUVs that underwent lipid mixing with CDVs. Those GUVs also exhibit esterase activity because cytosolic esterases entrapped in the CDVs are transferred during membrane fusion. Thus, content mixing is demonstrated. Using CDVs circumvents the need to purify or solubilize proteins. Moreover, calcium-mediated fusion allows transfer of lipids, water-soluble and membrane bound proteins in one step, resulting in a semi-synthetic cell.
Yannick R F Schmid, Leo Scheller, Sebastian Buchmann, Petra S Dittrich

2213 related Products with: Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins.

10000.1mg50050500500510001mg100100ul10

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#33083708   2020/08/22 To Up

Hereditary Angioedema Attack in Utero and Treatment of the Mother and Fetus.

Hereditary angioedema (HAE), an inherited deficiency of functional C1 esterase inhibitor (C1-INH), is characterized by unpredictable recurrent episodes of painful and often disabling swelling in subcutaneous and/or submucosal tissues. We report the case of a 23-year-old woman with type I HAE who had abdominal, facial, and peripheral attacks throughout her first pregnancy. A facial HAE attack occurred at week 38 of her pregnancy, and symptoms improved after self-administration of 50 U/kg of recombinant human C1-INH (total dose, 3500 U), but soon after she had an unusual abdominal sensation. Ultrasonography detected fetal lower lip swelling (∼3 times the normal size) and limb swelling. Physical examination of the mother found cervical dilatation, indicating the final stages of labor. Two hours after treatment of her HAE attack, she spontaneously delivered a healthy male infant. Photographs taken within 2 minutes of delivery revealed resolution of the infant's facial edema, and the limb edema was resolved within 30 minutes. By 10 minutes postdelivery, the mother's facial attack had almost completely resolved. Ten months after birth, genetic analysis confirmed that the infant had type I HAE. This is the first documented case of an HAE attack in utero. Treatment of the mother with recombinant human C1-INH was effective for the maternal and fetal attacks, with resolution within approximately 2 to 2.5 hours for both patients.
Vesna Grivcheva-Panovska, Bruno Giannetti

1510 related Products with: Hereditary Angioedema Attack in Utero and Treatment of the Mother and Fetus.

2.5 mg50 mg25 mg96T100ul100ug50 ug 10 mg1000 tests500 mg

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#33077275   2020/08/08 To Up

Increased bioavailability of phenolic acids and enhanced vascular function following intake of feruloyl esterase-processed high fibre bread: A randomized, controlled, single blind, crossover human intervention trial.

Clinical trial data have indicated an association between wholegrain consumption and a reduction in surrogate markers of cardiovascular disease. Phenolics present in wholegrain bound to arabinoxylan fibre may contribute these effects, particularly when released enzymatically from the fiber prior to ingestion. The aim of the present study was therefore to determine whether the intake of high fibre bread containing higher free ferulic acid (FA) levels (enzymatically released during processing) enhances human endothelium-dependent vascular function.
Alice L Turner, Louise V Michaelson, Peter R Shewry, Alison Lovegrove, Jeremy P E Spencer

2656 related Products with: Increased bioavailability of phenolic acids and enhanced vascular function following intake of feruloyl esterase-processed high fibre bread: A randomized, controlled, single blind, crossover human intervention trial.

10 0.1mg4 Membranes/Box4 Membranes/Box1ml200.00 ug1ml1 kit(96 Wells)4 Arrays/Slide100μg4 Membranes/Box4 Arrays/Slide

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#33075728   2020/10/16 To Up

Carbohydrate de-N-acetylases acting on structural polysaccharides and glycoconjugates.

Deacetylation of N-acetylhexosamine residues in structural polysaccharides and glycoconjugates is catalyzed by different families of carbohydrate esterases that, despite different structural folds, share a common metal-assisted acid/base mechanism with the metal cation coordinated with a conserved Asp-His-His triad. These enzymes serve diverse biological functions in the modification of cell-surface polysaccharides in bacteria and fungi as well as in the metabolism of hexosamines in the biosynthesis of cellular glycoconjugates. Focusing on carbohydrate de-N-acetylases, this article summarizes the background of the different families from a structural and functional viewpoint and covers advances in the characterization of novel enzymes over the last 2-3 years. Current research is addressed to the identification of new deacetylases and unravel their biological functions as they are candidate targets for the design of antimicrobials against pathogenic bacteria and fungi. Likewise, some families are also used as biocatalysts for the production of defined glycostructures with diverse applications.
Sergi Pascual, Antoni Planas

2662 related Products with: Carbohydrate de-N-acetylases acting on structural polysaccharides and glycoconjugates.

2.5 mg 100 UG100ug5ml1 g 2 ml Ready-to-use 96 wells (1 kit)100 mg100ug200 200ul100ug

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#33070853   2020/09/25 To Up

Detection of phthalate esters in PET bottled drinks and lake water using esterase/PANI/CNT/CuNP based electrochemical biosensor.

Phthalate esters (PEs) are the most common plasticizers that tends to exhibit endocrine disruption. Since, these PEs are used in the manufacture of PET bottles and PVC products: point of exposure magnifies up on consumption of PET bottle and plastic container stored drinking water and beverages. Apart from human exposure to PEs, bioaccumulation of PEs and toxic effects among wildlife also seems to be divergent. In the present study, an enzyme-based biosensor for the detection of PEs was developed to overcome the tedious extraction procedures involving PE extraction and sophisticated instruments for the detection. Linear Sweep voltammetry analysis of Nafion (NF) surface modified glassy carbon electrode with esterase (EST) and nano-components was carried-out. Peak potential of individual PEs were in the range of -1.72 to -1.82 V at the concentration of 1 × 10 mmol L. Sensitivity of EST/PANI/CNT/CuNP-NF modified GCE was determined in terms of detection limit and was calibrated to be 0.03-0.08 nmol L. Thus, the developed enzyme based electrochemical sensor could be successfully employed in determining PE exposure in humans and bioaccumulation among aquatic flora and fauna via., consumption of PET bottle stored drinks and industrial effluents discharged into the lakes.
Jayshree Annamalai, Namasivayam Vasudevan

2490 related Products with: Detection of phthalate esters in PET bottled drinks and lake water using esterase/PANI/CNT/CuNP based electrochemical biosensor.

100ul100μg100μg100μg100μg100μg1 mg100ul

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#33070050   2020/10/04 To Up

Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities.

The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea canephora and Coffea liberica, twelve were reported for the first time in endophytic association with coffee including members of the genera Brachybacterium, Caballeronia, Kitasatospora, Lechevalieria, Leifsonia, Luteibacter, Lysinibacillus, Mycolicibacterium, Nakamurella, Paracoccus, Sinomonas and Sphingobium. A total of eighty bacterial endophytes were characterized in vitro for several plant growth promoting and biocontrol traits including: the phosphate solubilization, the indolic compounds, siderophores, HCN, esterase, lipase, gelatinase and chitinase production. A subset of fifty selected bacteria were tested for their potential as biocontrol agents with in vitro confrontations with the fungal pathogen Fusarium oxysporum as well as the coffee parasitic nematodes Radopholus duriophilus and Pratylenchus coffeae. The three most efficient isolates on F. oxysporum belonging to the Bacillus, Burkholderia, and Streptomyces genera displayed a growth inhibition rate higher than 40%. Finally, five isolates from the Bacillus genus were able to lead to 100% of mortality in 24 h on both R. duriophilus and P. coffeae.
Duong Benoit, Nguyen Xuan Hoa, Phan Viet Ha, Colella Stefano, Trinh Quang Phap, Hoang Thi Giang, Nguyen Thi Tuyet, Marraccini Pierre, Lebrun Michel, Duponnois Robin

2060 related Products with: Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities.

5 G1000 2.5 mg100ul10 mg1,000 tests1 mg100 mg10mg96 tests1000 tests

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#33069201   2020/10/16 To Up

BCG vaccine, a ray of hope in treating Severe Acute Respiratory Syndrome (SARS).

In December 2019 in Wuhan, China, the Severe Acute Respiratory Syndrome - Corona Virus - 2 (SARS-CoV-2) (also 2019 nCoV) was first discovered. Later WHO declared that SARS-CoV-2 was responsible for Coronavirus Disease 2019 (COVID-19). The round, elliptical or pleomorphic shape of the 2019-nCoV is 60-140 nm in diameter. It comprises a single stranded genome of RNA that comprises 29891 nucleotides, a lipid shell and spike protein, envelops membrane, and hemagglutinin-esterase (HE) proteins. Several therapeutic approaches have been proposed to combat with this disease as this disease became global pandemic. Bacillus Calmette-Guérin (BCG) vaccine could be another approach and at present it is the only TB prevention vaccine approved. Both TB and COVID-19 causes severe pneumonia. In this review, we have tried to correlate the pathophysiology of both these disease condition. . In this review, we also have shown the history and current BCG practice throughout the world and it is hypothesized that the countries which strictly maintained and made mandatory call to recommend BCG vaccination are relatively less affected by COVID-19 pandemic. When BCG is exposed to a subsequent viral infection, it is allowed to lead to cross-reactive T cell responses which result in increased activity of Cluster of differentiation (CD) 4 + and Cluster of differentiation (CD) 8 + T cells and this may be a ray of hope in treating the SARS infection.
Amit Bandyopadhyay Banerjee, Thakur Gurjeet Singh, Abhimanyu Prashar

1168 related Products with: BCG vaccine, a ray of hope in treating Severe Acute Respiratory Syndrome (SARS).

100 μg5mg100.00 ug100ug100 μg100ug Lyophilized100 μg1 Set100 μg4 Arrays/Slide1 Set100 μg

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#33069130   2020/10/10 To Up

Chemical synthesis and biochemical characterization of cyclic oligonucleotides containing acyl groups at both 5'- and 3'-terminal positions.

Modified oligonucleotides, whose ON-OFF switch of hybridization can be controlled by an external stimulus, are important to understanding life phenomena and efficient treatment of diseases. The ON-OFF switch can be completely controlled by chemical modification of the oligonucleotide such as cyclization. However, their chemical modifications of the previous cyclic oligonucleotides remain after the addition of an external stimulus. To overcome this problem, we carried out the first synthesis of cyclic oligonucleotides containing acyl groups at both 5'- and 3'-terminal positions, which can be hydrolyzed by intracellular esterase. The cyclic oligonucleotides were successfully synthesized via disulfide bond formation and the phosphoramidite method without base protection on polymer supports containing a silyl linker. Subsequently, we were able to introduce a functional group into the cyclic oligonucleotide using the corresponding isothiocyanate reagent. Additionally, a cyclic oligonucleotide with acyl groups was found to have a much lower binding ability than the corresponding linear oligonucleotide. Moreover, we demonstrated its structural conversion to the corresponding linear oligonucleotide with two thiol groups under reducing conditions using dithiothreitol. It was also confirmed that the two terminal acyl groups of the linear oligonucleotide were hydrolyzed by pig liver esterase. These results indicate that hybridization of cyclic acylated nucleic acid drugs with high nuclease resistance is regulated by intracellular esterase under the reducing conditions in the cell cytoplasm.
Shuhei Nishizawa, Akihiro Ohkubo

1165 related Products with: Chemical synthesis and biochemical characterization of cyclic oligonucleotides containing acyl groups at both 5'- and 3'-terminal positions.

5 G 5 G100ug50 ug 25 mg96 wells (1 kit)100ul100ul100ug5mg1 g100ug

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