Search results for: frozen
#32791542 2020/08/13 To Up
Targeted RNAseq of Formalin-Fixed Paraffin-Embedded Tissue to Differentiate Among Benign and Malignant Adrenal Cortical Tumors.Lack of routine fresh or frozen tissue is a barrier to widespread transcriptomic analysis of adrenal cortical tumors and an impediment to translational research in endocrinology and endocrine oncology. Our group has previously pioneered the use of targeted amplicon-based next-generation sequencing for archival formalin-fixed paraffin-embedded (FFPE) adrenal tissue specimens to characterize the spectrum of somatic mutations in various forms of primary aldosteronism. Herein, we developed and validated a novel 194-amplicon targeted next-generation RNA sequencing (RNAseq) assay for transcriptomic analysis of adrenal tumors using clinical-grade FFPE specimens. Targeted RNAseq-derived expression values for 27 adrenal cortical tumors, including aldosterone-producing adenomas (APA; n=8), cortisol-producing adenomas (CPA; n=11), and adrenal cortical carcinomas (ACC; n=8), highlighted known differentially-expressed genes (DEGs; i. e., , , etc.) and tumor type-specific transcriptional modules (i. e., high cell cycle/proliferation transcript expression in ACC, etc.), and a subset of DEGs was validated orthogonally using quantitative reverse transcription PCR (qRT-PCR). Finally, unsupervised hierarchical clustering using a subset of high-confidence DEGs revealed three discrete clusters representing APA, CPA, and ACC tumors with corresponding unique gene expression signatures, suggesting potential clinical utility for a transcriptomic-based approach to tumor classification. Overall, these data support the use of targeted amplicon-based RNAseq for comprehensive transcriptomic profiling of archival FFPE adrenal tumor material and indicate that this approach may facilitate important translational research opportunities for the study of these tumors.
Samuel W Plaska, Chia-Jen Liu, Jung Soo Lim, Juilee Rege, Nolan R Bick, Antonio M Lerario, Gary D Hammer, Thomas J Giordano, Tobias Else, Scott A Tomlins, William E Rainey, Aaron M Udager
1041 related Products with: Targeted RNAseq of Formalin-Fixed Paraffin-Embedded Tissue to Differentiate Among Benign and Malignant Adrenal Cortical Tumors.0.1ml (1mg/ml)5 Pieces/Box50 samples
#32791377 2020/06/06 To Up
Porcine oviductal extracellular vesicles interact with gametes and regulate sperm motility and survival.Once in the female reproductive tract, spermatozoa undergo several modifications to acquire their complete fertilizing ability. Interactions between the oviductal fluid (OF) and gametes contribute to a successful fertilization. Recently, oviductal extracellular vesicles have been identified as an important part of the OF but their interactions with gametes are not fully understood. In the present study, we aim at determining the patterns of interactions between porcine oviductal extracellular vesicles (poEVs) and gametes (spermatozoa and oocytes). Moreover, we evaluate the effect of poEVs on sperm survival and motility to better understand the mechanisms by which poEVs modulate the processes leading to fertilization. Evaluation of poEVs uptake by spermatozoa showed that poEVs bind to spermatozoa in a time and dose dependent manner. Co-incubation of spermatozoa with poEVs (0.2 μg/μL) increased fresh and frozen sperm survival after 6 and 17 h, respectively. By contrast, poEVs supplementation reduced the total and progressive sperm motility after 2 h. Additionally, we demonstrated that poEVs interacted with the cumulus cells, zona pellucida (ZP) and oocyte, being able to cross the ZP. Besides, we showed that poEVs delivered their cargo into the oocyte, by the transfer of OVGP1 protein. In conclusion, our results demonstrated that poEVs are able to interact with both gametes. Besides, the findings from the present study showed that poEVs may participate in maintaining sperm viability and reducing motility, functions associated with the oviduct sperm reservoir. Although further investigations are needed, our results indicate that poEVs can be a potential tool to improve sperm life span during sperm handling and enhance IVF outcomes.
Agostinho S Alcântara-Neto, Lorraine Schmaltz, Erika Caldas, Marie-Claire Blache, Pascal Mermillod, Carmen Almiñana
1902 related Products with: Porcine oviductal extracellular vesicles interact with gametes and regulate sperm motility and survival.1 ml1 g96 wells (1 kit)50 ug 100 mg200ul100ug10 mg 25 MG25 mg100 mg10 mg
#32791302 2020/08/10 To Up
Single nuclei RNA sequencing assessment of the hepatic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin.Characterization of cell specific transcriptional responses to hepatotoxicants is lost in the averages of bulk RNA-sequencing (RNA-seq). Single-cell/nuclei RNA-seq technologies enable the transcriptomes of individual cell (sub)types to be assessed within the context of in vivo models.
Rance Nault, Kelly A Fader, Sudin Bhattacharya, Tim R Zacharewski
1719 related Products with: Single nuclei RNA sequencing assessment of the hepatic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin.200 units200 units 5 G100ml5 mg2100 extractions 1ml0,5 ML200ul250ul
#32790892 2020/08/13 To Up
Erythropoietin plus iron versus control treatment including placebo or iron for preoperative anaemic adults undergoing non-cardiac surgery.Approximately 30% of adults undergoing non-cardiac surgery suffer from preoperative anaemia. Preoperative anaemia is a risk factor for mortality and adverse outcomes in different surgical specialties and is frequently the reason for blood transfusion. The most common causes are renal, chronic diseases, and iron deficiency. International guidelines recommend that the cause of anaemia guide preoperative anaemia treatment. Recombinant human erythropoietin (rHuEPO) with iron supplementation has frequently been used to increase preoperative haemoglobin concentrations in patients in order to avoid the need for perioperative allogeneic red blood cell (RBC) transfusion.
Lutz Kaufner, Christian von Heymann, Anne Henkelmann, Nathan L Pace, Stephanie Weibel, Peter Kranke, Joerg J Meerpohl, Ravi Gill
1704 related Products with: Erythropoietin plus iron versus control treatment including placebo or iron for preoperative anaemic adults undergoing non-cardiac surgery.2.50 nmol 100ul1 kg
#32790697 2020/08/13 To Up
Pseudorandom sequence contention algorithm for IEEE 802.11ah based internet of things network.The IEEE 802.11ah standard relies on the conventional distributed coordination function (DCF) as a backoff selection method. The DCF is utilized in the contention-based period of the newly introduced medium access control (MAC) mechanism, namely restricted access window (RAW). Despite various advantages of RAW, DCF still utilizes the legacy binary exponential backoff (BEB) algorithm, which suffers from a crucial disadvantage of being prone to high probability of collisions with high number of contending stations. To mitigate this issue, this paper investigates the possibility of replacing the existing exponential sequence (i.e., as in BEB) with a better pseudorandom sequence of integers. In particular, a new backoff algorithm, namely Pseudorandom Sequence Contention Algorithm (PRSCA) is proposed to update the CW size and minimize the collision probability. In addition, the proposed PRSCA incorporates a different approach of CW freezing mechanism and backoff stage reset process. An analytical model is derived for the proposed PRSCA and presented through a discrete 2-D Markov chain model. Performance evaluation demonstrates the efficiency of the proposed PRSCA in reducing collision probability and improving saturation throughput, network throughput, and access delay performance.
Mohammed A Raouf, Fazirulhisyam Hashim, Jiun Terng Liew, Kamal Ali Alezabi
1681 related Products with: Pseudorandom sequence contention algorithm for IEEE 802.11ah based internet of things network.100 mg0.2 mg100 µg250 mg96 Tests0.1 mgOne 96-Well Microplate Ki100 mg 1000 ml 100ug
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#32789763 2020/08/13 To Up
Signet-ring cell appearance of atrophic fat cells.Cells with 'signet-ring' appearance were found at post-mortem examination of a man with a history of chronic illness, weight loss and multiple regions of 'bowel thickening' during life. Due to the decedent's history, the finding raised the possibility of disseminated signet-ring adenocarcinoma. However, the vacuoles did not stain for mucin and the cells did not stain for keratin. The cells did stain for calretinin and so a diagnosis of signet ring mesothelioma was considered. However, it was suggested that the cells with a cytoplasmic vacuole displacing the nucleus to one side producing the signet-ring appearance were instead atrophic fat cells. This was subsequently proven by Oil Red O staining.
John D Gilbert, Neil E I Langlois, Andrew Ruszkiewicz, David Moffat, Kathy Cash96 tests1 mg2 ml1.00 flask96 rxns 1 kit(s) 15ml1mg1.5x10(6) cells25 96 tests1.00 flask
#32789596 2020/08/13 To Up
The proximal humeral attachment of the lateral head of the triceps brachii: a cadaveric study and potential site for radial nerve compression.Knowledge of potential compression sites of peripheral nerves is important to the clinician and surgeon alike. One anatomical location for potential compression of the radial nerve, which is rarely mentioned in the literature, is at the proximal humeral attachment of the lateral head of the triceps brachii at the level of the proximal spiral groove. As no anatomical studies have been devoted to this band, the present study was conducted.
Skyler Jenkins, Stephen Bordes, Łukasz Olewnik, Robert Haładaj, Joe Iwanaga, Marios Loukas, Aaron S Dumont, C J Bui, R Shane Tubbs
2585 related Products with: The proximal humeral attachment of the lateral head of the triceps brachii: a cadaveric study and potential site for radial nerve compression.0.1 mg100.00 ul 100ul1 ml100.00 ul
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#32789285 2020/08/10 To Up
Cold acclimation has a differential effect on leaf vascular bundle structure and carbon export rates in natural accessions originating from southern and northern Europe.Acclimation to low but non-freezing temperature represents an ecologically important process for but also for many other plant species from temperate regions. Cold acclimation comprises and affects numerous molecular and physiological processes and the maintenance of sugar supply of sink tissue by photosynthetically active source tissue is essential for plant survival. Here, changes in vascular bundle (VB) structure at the leaf petiole were analysed together with sucrose exudation rates before and after cold acclimation. Six natural accessions originating from southern and northern Europe were compared. Photosynthetic efficiency, that is, maximum and effective quantum yield of photosystem II, revealed a significant effect of environmental condition. Only for northern accessions was a highly significant negative correlation observed between leaf sucrose exudation rates, xylem, and petiole cross-sectional areas. Furthermore, only for northern accessions was a significant increase of VB and leaf petiole cross-sectional area observed during cold acclimation. In contrast, variance of cross-sectional areas of cold acclimated southern accessions was strongly reduced compared to control plants, while mean areas remained similar under both conditions. In summary, these findings suggest that natural accessions from northern Europe significantly adjust sink strength and leaf VB structure to maintain plant growth and photosynthesis under low temperature.
Katja Schneider, Lorena Abazaj, Cornelia Niemann, Laura Schröder, Thomas Nägele
1000 related Products with: Cold acclimation has a differential effect on leaf vascular bundle structure and carbon export rates in natural accessions originating from southern and northern Europe.5 G200 100ug25 mg 5 G1 g1 ml96 wells (1 kit)100ul100ul100ug200ul
#32789180 2020/07/31 To Up
Controlling ice formation on gradient wettability surface for high-performance bioinspired materials.Ice-templating holds promise to become a powerful technique to construct high-performance bioinspired materials. Both ice nucleation and growth during the freezing process are crucial for the final architecture of the ice-templated material. However, effective ways to control these two very important factors are still lacking. Here, we demonstrate that successive ice nucleation and preferential growth can be realized by introducing a wettability gradient on a cold finger. A bulk porous material with a long-range lamellar pattern was obtained using a linear gradient, yielding a high-performance, bulk nacre-mimetic composite with excellent strength and toughness after infiltration. In addition, cross-aligned and circular lamellar structures can be obtained by freeze-casting on surfaces modified with bilayer linear gradient and radial gradient, respectively, which are impossible to realize with conventional freeze-casting techniques. Our study highlights the potential of harnessing the rich designability of surface wettability patterns to build high-performance bulk materials with bioinspired complex architectures.
Nifang Zhao, Meng Li, Huaxin Gong, Hao Bai
1688 related Products with: Controlling ice formation on gradient wettability surface for high-performance bioinspired materials.50 assays96 Tests1 mg100ug100ug100ug Lyophilized1 mg5 mg500 g100 assays
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