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#33085535   2020/10/20 To Up

The Effects of Storage Time and Repeated Freeze-Thaw Cycles on Intact Fibroblast Growth Factor 23 Levels.

Fibroblast growth factor 23 (FGF23) has become increasingly important in chronic kidney diseases (CKDs), cardiovascular calcification, and metabolic bone diseases. Fresh or stored blood samples are widely used for the FGF23 assay. Clarifying the factors influencing the FGF23 assay can help to quantify FGF23 more accurately. This study explored the effects of low-temperature storage time and repeated freeze-thaw cycles on the measurement of serum intact FGF23 (iFGF23). We selected 60 serum samples from patients with CKD stages 3-5 and hemodialysis patients. An enzyme-linked immunosorbent assay was used to measure the changes in serum iFGF23 levels after 6 years of storage at -80°C. In total, 18 fresh serum samples were frozen and thawed for 0, 1, 3, and 5 cycles to explore the effects of repeated freeze-thaw cycles on serum iFGF23 levels. Median serum iFGF23 concentrations were 252.17 (interquartile range [IQR] 113.82-592.38) pg/mL and 203.85 (IQR 64.76-545.39) pg/mL before and after 6 years. There were no significant differences between them. However, we found a downward trend of 48% in the samples close to the normal level of iFGF23 (<150.34 pg/mL) after 6 years of storage ( = 0.160). In addition, the iFGF23 levels of samples frozen and thawed for 0, 1, 3, and 5 cycles were 278.41 ± 39.51 (mean ± standard deviation) pg/mL, 262.84 ± 38.42 pg/mL, 252.97 ± 34.65 pg/mL and 250.49 ± 37.12 pg/mL, respectively. A slight downward trend in iFGF23 levels was observed with increasing freeze-thaw times; however, no significant differences were found among different freeze-thaw cycles. Serum iFGF23 levels remained stable after storage at -80°C for 6 years. In addition, five freeze-thaw cycles had no significant effects on serum iFGF23 levels.
Rong Tang, Yinghui Lu, Ru Yin, Ping Zhu, Ling Zhu, Chunxia Zheng

1803 related Products with: The Effects of Storage Time and Repeated Freeze-Thaw Cycles on Intact Fibroblast Growth Factor 23 Levels.

96 wells (1 kit)0.1 mg96 wells (1 kit)0.1 mg5ug0.1 mg10ug96 wells (1 kit)2ug0.1 mg0.1 mg2ug x 20

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#33085024   2020/10/21 To Up

Comparative methods for fecal sample storage to preserve gut microbial structure and function in an in vitro model of the human colon.

In vitro gut models, such as the mucosal artificial colon (M-ARCOL), provide timely and cost-efficient alternatives to in vivo assays allowing mechanistic studies to better understand the role of human microbiome in health and disease. Using such models inoculated with human fecal samples may require a critical step of stool storage. The effects of preservation methods on microbial structure and function in in vitro gut models have been poorly investigated. This study aimed to assess the impact of three commonly used preserving methods, compared with fresh fecal samples used as a control, on the kinetics of lumen and mucus-associated microbiota colonization in the M-ARCOL model. Feces from two healthy donors were frozen 48 h at - 80 °C with or without cryoprotectant (10% glycerol) or lyophilized with maltodextrin and trehalose prior to inoculation of four parallel bioreactors (e.g., fresh stool, raw stool stored at - 80 °C, stool stored at - 80 °C with glycerol and lyophilized stool). Microbiota composition and diversity (qPCR and 16S metabarcoding) as well as metabolic activity (gases and short chain fatty acids) were monitored throughout the fermentation process (9 days). All the preservative treatments allowed the maintaining inside the M-ARCOL of a complex and functional microbiota, but considering stabilization time of microbial profiles and activities (and not technical constraints associated with the supply of frozen material), our results highlighted 48 h freezing at - 80 °C without cryoprotectant as the most efficient method. These results will help scientists to determine the most accurate method for fecal storage prior to inoculation of in vitro gut microbiome models. KEY POINTS: • In vitro ARCOL model reproduces luminal and mucosal human microbiome. • Short-term storage of fecal sample influences microbial stabilization and activity. • 48 h freezing at - 80°C: most efficient method to preserve microbial ecosystem. • Scientific and technical requirements: influencers of preservation method.
Charlotte Deschamps, Elora Fournier, Ophélie Uriot, Frédérique Lajoie, Cécile Verdier, Sophie Comtet-Marre, Muriel Thomas, Nathalie Kapel, Claire Cherbuy, Monique Alric, Mathieu Almeida, Lucie Etienne-Mesmin, Stéphanie Blanquet-Diot

2411 related Products with: Comparative methods for fecal sample storage to preserve gut microbial structure and function in an in vitro model of the human colon.

8 Sample Kit4 Sample Kit4 Sample Kit96T8 Sample Kit16 Arrays/Slide4 Sample Kit16 Arrays/Slide8 Sample Kit

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#33084562   // To Up

Cryoprecipitate transfusion in bleeding patients.

The management of acquired coagulopathy in multiple clinical settings frequently involves fibrinogen supplementation. Cryoprecipitate, a multidonor product, is widely used for the treatment of acquired hypofibrinogenemia following massive bleeding, but it has been associated with adverse events. We aimed to review the latest evidence on cryoprecipitate for treatment of bleeding.
Barto Nascimento, Jerrold H Levy, Homer Tien, Luis Teodoro Da Luz

2563 related Products with: Cryoprecipitate transfusion in bleeding patients.

0.1mg5mg100 μgInhibitor100 μg100ug Lyophilized100 μg50ul

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#33083861   2020/10/20 To Up

Double trouble? Impact of frozen embryo transfer on the monozygotic twinning rate: a retrospective cohort study from 8459 cycles.

To compare monozygotic twinning (MZT) rates in patients undergoing fresh embryo transfer (ET) and frozen embryo transfer.
Xitong Liu, Ping Li, Juanzi Shi

1076 related Products with: Double trouble? Impact of frozen embryo transfer on the monozygotic twinning rate: a retrospective cohort study from 8459 cycles.

0.1ml

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#33083466   2020/10/02 To Up

Roxb. Expedites the Healing Process in Contact Frostbite.

Frostbite is caused due to extreme vulnerability to cold, resulting in damage of deeper and superficial tissues alike. In this study, we report the anti-inflammatory and wound-healing properties of aqueous methanolic extract of () against contact frostbite. Thirty rats were divided into five groups including three treatment groups with increasing doses of , a standard drug group receiving acetylsalicylic acid (ASA), and a metal bar-induced frostbite group. Frostbite injury was induced by a 3 × 3.5 cm metal bar frozen up to -79°C on shaved skin for continuous 3 minutes. Wounded area percentages were recorded to measure the healing rate in response to administration. Haematological parameters and malondialdehyde content were also noted. On treatment with , the healing rate is drastically increased and lipid peroxidation product malondialdehyde was decreased in a dose-dependent manner. Results were compared with frostbite and ASA (standard drug group). These results indicate that possesses excellent wound-healing properties against frostbite injury and can prove to be a prospective compound in such conditions.
Waseem Hassan, Manal Ali Buabeid, Umme Kalsoom, Sahar Bakht, Imran Akhtar, Furqan Iqbal, El-Shaimaa A Arafa

2453 related Products with: Roxb. Expedites the Healing Process in Contact Frostbite.

100 units196 tests96 wells

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#33083210   2020/10/15 To Up

Drop for drop: A descriptive analysis of blood product usage in a South African tertiary care setting during the Covid-19 pandemic.

The Covid-19 pandemic has had a drastic effect on the global community. Blood products are precious resources especially in the African context and this has been especially compounded during the Covid-19 pandemic. Concurrent to this during the Covid-19 level 5 lockdown in South Africa from 26 March - 30 April 2020, a decrease in trauma admissions to state hospitals was noted. The aim of this data collection was to assess whether lowered blood product issuance was seen during the Covid-19 pandemic lockdown.
D C Shead

2807 related Products with: Drop for drop: A descriptive analysis of blood product usage in a South African tertiary care setting during the Covid-19 pandemic.

25 µg 100 UG 50 UG 50 UG100 TESTScase0.1 mg

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#33081373   2020/10/16 To Up

A Python-Based Pipeline for Preprocessing LC-MS Data for Untargeted Metabolomics Workflows.

Preprocessing data in a reproducible and robust way is one of the current challenges in untargeted metabolomics workflows. Data curation in liquid chromatography-mass spectrometry (LC-MS) involves the removal of biologically non-relevant features (retention time, pairs) to retain only high-quality data for subsequent analysis and interpretation. The present work introduces TidyMS, a package for the Python programming language for preprocessing LC-MS data for quality control (QC) procedures in untargeted metabolomics workflows. It is a versatile strategy that can be customized or fit for purpose according to the specific metabolomics application. It allows performing quality control procedures to ensure accuracy and reliability in LC-MS measurements, and it allows preprocessing metabolomics data to obtain cleaned matrices for subsequent statistical analysis. The capabilities of the package are shown with pipelines for an LC-MS system suitability check, system conditioning, signal drift evaluation, and data curation. These applications were implemented to preprocess data corresponding to a new suite of candidate plasma reference materials developed by the National Institute of Standards and Technology (NIST; hypertriglyceridemic, diabetic, and African-American plasma pools) to be used in untargeted metabolomics studies in addition to NIST SRM 1950 Metabolites in Frozen Human Plasma. The package offers a rapid and reproducible workflow that can be used in an automated or semi-automated fashion, and it is an open and free tool available to all users.
Gabriel Riquelme, Nicolás Zabalegui, Pablo Marchi, Christina M Jones, María Eugenia Monge

1408 related Products with: A Python-Based Pipeline for Preprocessing LC-MS Data for Untargeted Metabolomics Workflows.

1 G 1 G20 ug100μg25 µg1 kit0.1 mg1 mg250 mg

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