Search results for: Rat anti mouse CD205 (a dendritic cell marker)
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In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells.
Targeting of antigen to dendritic cells (DCs) increase the efficiency of immunization procedures and may facilitate the development of more effective vaccines. Several surface molecules on DCs have shown to be useful for antigen targeting, but many more deserves investigation for their efficacy in this respect. With this end in mind, a simple in vitro assay for screening of optimal targets for antigen-delivery to murine DCs was established. Splenocytes from mice immunized with rat IgG were targeted in vitro with a panel of different rat monoclonal antibodies (mAbs) directed against surface markers on murine DCs. The resulting T-cell activation was analysed by determining the number of IFN-γ and IL-4 secreting cells by ELISPOT. A positive effect of targeting was evident with several of the mAbs. Thus, mAbs against CD11c, CD36, CD205 and Clec7A all induced IFN-γ responses that were significantly higher than those induced by non-targeting control mAbs. Anti-CD36 also induced IL-4 responses that were significantly higher than the control. The assay described here allows simultaneous analysis of a large number of potential target structures and facilitates direct comparison between the different targets regarding the strength of the T-cell responses induced by the targeted DCs. The assay could be useful as a first-line screening of potential target structures on murine DCs.L H Pugholm, K Varming, R Agger
1932 related Products with: In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells.
50 assays1 kit(96 Wells)96 Tests1 kit(96 Wells)1 kit500 MG1 kit(96 Wells)96 assays100 assays100 assays100 assays100 assays
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#25334032 2014/10/21
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Three distinct subsets of thymic epithelial cells in rats and mice defined by novel antibodies.
Thymic epithelial cells (TECs) are thought to play an essential role in T cell development and have been detected mainly in mice using lectin binding and antibodies to keratins. Our aim in the present study was to create a precise map of rat TECs using antibodies to putative markers and novel monoclonal antibodies (i.e., ED 18/19/21 and anti-CD205 antibodies) and compare it with a map from mouse counterparts and that of rat thymic dendritic cells.Yasushi Sawanobori, Hiashi Ueta, Christine D Dijkstra, Chae Gyu Park, Motoyasu Satou, Yusuke Kitazawa, Kenjiro Matsuno