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#32473569   2020/05/27 To Up

Detection of ctDNA with Personalized Molecular Barcode NGS and Its Clinical Significance in Patients with Early Breast Cancer.

We attempted to detect circulating tumor DNA (ctDNA), taking advantage of molecular barcode next-generation sequencing (MB-NGS), which can be more easily customized to detect a variety of mutations with a high sensitivity than PCR-based methods. Sequencing with a gene panel consisting of the 13 most frequently mutated genes in breast tumors from stage I or II patients revealed 95 somatic mutations in the 12 genes in 62% (62/100) of tumors. Then, plasma DNA from each patient (n = 62) before surgery was analyzed via MB-NGS customized to each somatic mutation, resulting in the detection of ctDNA in 16.1% (10/62) of patients. ctDNA was significantly associated with biologically aggressive phenotypes, including large tumor size (P = .004), positive lymph node (P = .009), high histological grade (P < .001), negative ER (P = .018), negative PR (P = .017), and positive HER2 (P = .046). Furthermore, distant disease-free survival was significantly worse in patients with ctDNA (n = 10) than those without ctDNA (n = 52) (P < .001). Our results demonstrate that MB-NGS personalized to each mutation can detect ctDNA with a high sensitivity in early breast cancer patients at diagnosis, and it seems to have a potential to serve as a clinically useful tumor marker for predicting their prognosis.
Tetsuhiro Yoshinami, Naofumi Kagara, Daisuke Motooka, Shota Nakamura, Tomohiro Miyake, Tomonori Tanei, Yasuto Naoi, Masafumi Shimoda, Kenzo Shimazu, Seung Jin Kim, Shinzaburo Noguchi

1187 related Products with: Detection of ctDNA with Personalized Molecular Barcode NGS and Its Clinical Significance in Patients with Early Breast Cancer.



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#32473554   2020/05/15 To Up

Complexities in the diagnosis of large B-cell lymphomas, classic Hodgkin lymphomas and overlapping peripheral T-cell lymphomas simplified: An evidence-based guide.

The diagnosis of a large B-cell lymphoma and classic Hodgkin lymphoma (CHL) is often straightforward. However, in select circumstances, these simple diagnoses can be quite complex. In part, diagnostic difficulty may be due to uncertainty in the evaluation of morphologic and immunophenotypic features along a biologic continuum, or alternatively arise from uncertainty in predicting the behavior and outcomes of patients. Here, we systematically discuss and review areas of diagnostic difficulty in the diagnosis of large B-cell lymphomas (LBCL), classic Hodgkin lymphomas (CHL) and peripheral T-cell lymphomas (PTCL). We provide careful data-driven analyses and evidence-based approaches to help guide pathologists and clinicians. We discuss: 1) marginal zone lymphomas with increased large cells versus diffuse large B-cell lymphoma (DLBCL), 2) chronic lymphocytic leukemia with expanded proliferation centers versus diffuse large B-cell lymphoma (DLBCL), 3) chronic lymphocytic leukemia with Hodgkin/Reed-Sternberg-like cells versus CHL arising from chronic lymphocytic leukemia, 4) complex cases of follicular lymphoma versus DLBCL, 5) PTCL with large B-cell proliferations versus PTCL with LBCL, 6) PTCL with Hodgkin/Reed-Sternberg-like cells versus CHL, and finally 7) blastoid/pleomorphic mantle cell lymphoma versus DLBCL. Our evidence and data driven approach may serve as a useful diagnostic guide.
Kwun Wah Wen, Bita Fakhri, Joshua Menke, Roberto Ruiz-Cordero, Ryan M Gill, Robert S Ohgami

2436 related Products with: Complexities in the diagnosis of large B-cell lymphomas, classic Hodgkin lymphomas and overlapping peripheral T-cell lymphomas simplified: An evidence-based guide.

1 mL100ug Lyophilized100ug Lyophilized100ug Lyophilized100 μg100ug Lyophilized2 mL100 µg100ug Lyophilized100ug Lyophilized

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#32473442   2020/05/13 To Up

Methodology for forecast and control of coastal harmful algal blooms by embedding a compound eutrophication index into the ecological risk index.

Harmful algae bloom (HAB) is a major global ecological hazard and is a serious problem in the Bohai Sea. There have been few successful controls of HABs associated with HAB accurate predictions due to a lack of link between ecological risks and control measures. A methodology is proposed that embeds the compound eutrophication index (CEI) into an ecological risk index (ERI) for HAB prediction, which can define critical factors associated with measures of HAB control. CEI can be calculated by means of a function with 15 control elements. These are multiplied with the occurrence probability and ecosystem vulnerability to HAB events to calculate the ERI of HAB. Based on the results of CEI and ERI, it has experienced eutrophication and has been at a high-risk state since 1989 in the Bohai Sea. There is good correlation between CEI and chlorophyll a concentration, and HAB risk evaluation in accordance with ERI embedded CEI is considerable reliability in both location and time in the Bohai Sea. The ERI value averages 24% ± 35% with peak values (73% ± 4.3%) in summer, and high values (at the level of grade III of ERI, 6%) are mostly in Bohai Bay, Laizhou Bay, Liaodong Bay and the coastal sea waters of Qinhuangdao city. The contribution of terrigenous pollutant emission and concentration effects to the ERI is 63%, with reclamation and hydrodynamic effects accounting for 22%, and runoff and sediment effects accounting for 15%. Thus, actions associated with terrigenous pollutant emission/concentration would be more effective than other measures in prevention and control of HAB.
Guohong Lin, Xuefeng Xu, Ping Wang, Shengkang Liang, Yanbin Li, Ying Su, Keqiang Li, Xiulin Wang

1836 related Products with: Methodology for forecast and control of coastal harmful algal blooms by embedding a compound eutrophication index into the ecological risk index.

10 assays100ul100ug Lyophilized110 mg 0.1 mg

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#32473318   2020/05/27 To Up

The impact of synthetic amorphous silica (E 551) on differentiated Caco-2 cells, a model for the human intestinal epithelium.

For several decades, food-grade synthetic amorphous silica (SAS) have been used as a technological additive to reduce caking of food powders. Human exposure is thus inevitable and safety concerns are taken seriously. The toxicity of silica in general and SAS in particular has been studied extensively. Overall, there is little evidence that food-grade SAS pose any health risks to humans. However, from the available data it was often not clear which type of silica was used. Accordingly, the latest report of the European food safety authority requested additional toxicity data for well-characterised "real food-grade SAS". To close this gap, we screened a panel of ten well-defined, food-grade SAS for potential adverse effects on differentiated Caco-2 cells. Precipitated and fumed SAS with low, intermediate and high specific surface area were included to determine structure-activity relationships. In a physiological dose-range up to 50 μg/ml and 48 h of incubation, none of the materials induced adverse effects on differentiated Caco-2 cells. This held true for endpoints of acute cytotoxicity as well as epithelial specific measures of barrier integrity. These results showed that despite considerable differences in production routes and material characteristics, food-relevant SAS did not elicit acute toxicity responses in intestinal epithelial cells.
Claudia Hempt, Jean-Pierre Kaiser, Olivier Scholder, Tina Buerki-Thurnherr, Heinrich Hofmann, Alexandra Rippl, Tobias B Schuster, Peter Wick, Cordula Hirsch

2235 related Products with: The impact of synthetic amorphous silica (E 551) on differentiated Caco-2 cells, a model for the human intestinal epithelium.

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#32473302   2020/05/27 To Up

Prognostic gene expression signature for high-grade serous ovarian cancer.

Median overall survival (OS) for women with high-grade serous ovarian cancer (HGSOC) is approximately four years, yet survival varies widely between patients. There are no well-established, gene expression signatures associated with prognosis. The aim of this study was to develop a robust prognostic signature for overall survival in HGSOC patients.
J Millstein, T Budden, E L Goode, M S Anglesio, A Talhouk, M P Intermaggio, H S Leong, S Chen, W Elatre, B Gilks, T Nazeran, M Volchek, R C Bentley, C Wang, D S Chiu, S Kommoss, S C Y Leung, J Senz, A Lum, V Chow, H Sudderuddin, R Mackenzie, J George, , S Fereday, J Hendley, N Traficante, H Steed, J M Koziak, M Köbel, I A McNeish, T Goranova, D Ennis, G Macintyre, D Silva, T Ramón Y Cajal, J García-Donas, S Hernando Polo, G C Rodriguez, K L Cushing-Haugen, H R Harris, C S Greene, R A Zelaya, S Behrens, R T Fortner, P Sinn, E Herpel, J Lester, J Lubiński, O Oszurek, A Tołoczko, C Cybulski, J Menkiszak, C L Pearce, M C Pike, C Tseng, J Alsop, V Rhenius, H Song, M Jimenez-Linan, A Piskorz, A Gentry-Maharaj, C Karpinskyj, M Widschwendter, N Singh, C J Kennedy, R Sharma, P R Harnett, B Gao, S E Johnatty, R Sayer, J Boros, S J Winham, G L Keeney, S H Kaufmann, M C Larson, H Luk, B Y Hernandez, P J Thompson, L R Wilkens, M E Carney, B Trabert, J Lissowska, L Brinton, M E Sherman, C Bodelon, S Hinsley, L A Lewsley, R Glasspool, S N Banerjee, E A Stronach, P Haluska, I Ray-Coquard, S Mahner, B Winterhoff, D Slamon, D A Levine, L E Kelemen, J Benitez, J Chang-Claude, J Gronwald, A H Wu, U Menon, M T Goodman, J M Schildkraut, N Wentzensen, R Brown, A Berchuck, G Chenevix-Trench, A deFazio, S A Gayther, M J García, M Henderson, M A Rossing, A Beeghly-Fadiel, P A Fasching, S Orsulic, B Y Karlan, G E Konecny, D G Huntsman, D D Bowtell, J D Brenton, J A Doherty, P D P Pharoah, S J Ramus

2835 related Products with: Prognostic gene expression signature for high-grade serous ovarian cancer.



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