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#32781325   2020/08/06 To Up

NIR fluorescence for monitoring in vivo scaffold degradation along with stem cell tracking in bone tissue engineering.

Stem cell-based tissue engineering has the potential to use as an alternative for autologous tissue grafts; however, the contribution of the scaffold degradation along with the transplanted stem cells to in vivo tissue regeneration remains poorly understood. Near-infrared (NIR) fluorescence imaging has great potential to monitor implants while avoiding autofluorescence from the adjacent host tissue. To utilize NIR imaging for in vivo monitoring of scaffold degradation and cell tracking, we synthesized 800-nm emitting NIR-conjugated PCL-ran-PLLA-ran-PGA (ZW-PCLG) copolymers with three different degradation rates and labeled 700-nm emitting lipophilic pentamethine (CTNF127) on the human placental stem cells (CT-PSCs). The 3D bioprinted hybrid constructs containing the CT-PSC-laden hydrogel together with the ZW-PCLG scaffolds demonstrate that NIR fluorescent imaging enables tracking of in vivo scaffold degradation and stem cell fate for bone regeneration in a rat calvarial bone defect model. This NIR-based monitoring system can be effectively utilized to study cell-based tissue engineering applications.
Soon Hee Kim, Ji Hoon Park, Jin Seon Kwon, Jae Gu Cho, Kate G Park, Chan Hum Park, James J Yoo, Anthony Atala, Hak Soo Choi, Moon Suk Kim, Sang Jin Lee

2463 related Products with: NIR fluorescence for monitoring in vivo scaffold degradation along with stem cell tracking in bone tissue engineering.

1 mg900 tests

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#32781270   2020/07/25 To Up

Limb differences in hamstring muscle function and morphology after anterior cruciate ligament reconstruction.

To compare inter-limb differences in hamstring strength, muscle volume, and neural activity between individuals with anterior cruciate ligament reconstruction (ACLR) and healthy controls, and to identify associations between hamstring neuromuscular function and subjective knee function.
Justin L Rush, Grant E Norte, Adam S Lepley

2298 related Products with: Limb differences in hamstring muscle function and morphology after anterior cruciate ligament reconstruction.

96 wells96 wells5mg1 mg100ug Lyophilized0.1ml100ug

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#32781209   2020/08/08 To Up

Effects of three medicinal herbs Bidens pilosa, Lonicera japonica, and Cyathula officinalis on growth and non-specific immune responses of cobia (Rachycentron canadum).

This study investigates the effects of three medicinal herbal extracts, namely Bidens pilosa (BPE), Lonicera japonica (LJE), and Cyathula officinalis (COE), on nonspecific immune parameters of cobia (Rachycentron canadum) in vitro and in vivo. During in vitro tests, BPE treatment increased reactive oxygen species (ROS) production in a dose-dependent manner in primary head kidney leukocytes. Similarly, ROS production rates were enhanced by LJE (50 and 100 mg/ml) and COE (100 mg/ml). This suggests that these three herbal extracts possess immunostimulating properties. We then conducted two feeding trials to examine the effects of these three herbal extracts on growth and innate immune parameters of cobia, and sought an optimal dietary supplementation proportion required for activating the non-specific immune responses. In the first trial, we supplemented the diet with 1, 5, or 10% of the individual extracts. After a ten-week feeding trial, no negative impacts on weight gain, feed conversion rate, and survival rate were observed in fish offered experimental diets. Further, ROS production, phagocytic capacity of the head kidney leukocytes, and serum lysozyme activity were enhanced by differing degrees in fish fed the herbal extracts compared to fish in the control group. A similar albumin/globulin ratio was seen between each experimental group and the control group regardless of the type and dose of herbal extract used, indicating these medicinal herbal extracts are safe for cobia. We then performed a 30-day feeding trial with lower extract concentrations (1, 3, and 5% of the diet) to identify dose responses in cobia at various time points so that we could establish a cost-effective manner of administering the three extracts for cobia. All BPE fed fish had higher ROS production compared to the control group, while phagocytosis rate and index were simultaneously raised in only the BPE30 group (3% BPE). Immune parameters such as ROS production, phagocytic rate, and serum lysozyme activity were triggered when fish received 30 g LJE per kg of feed. However, ROS production only increased in the LJE10 group (1% LJE) on day 30 and was not enhanced in the LJE50 group (5% LJE). Additionally, although the phagocytic rate and phagocytic index were induced in the LJE50 group, serum lysozyme activity was not elevated in this group (LJE50) at any time point examined. ROS production was greatly improved in all COE fed groups, but only the COE30 group (3% COE) showed prolonged enhanced phagocytic rate over the 30-day feeding trial.
Po-Tsang Lee, Hsiang-Yin Chen, Zhen-Hao Liao, Huai-Ting Huang, Ting-Chieh Chang, Cheng-Ting Huang, Meng-Chou Lee, Fan-Hua Nan

2859 related Products with: Effects of three medicinal herbs Bidens pilosa, Lonicera japonica, and Cyathula officinalis on growth and non-specific immune responses of cobia (Rachycentron canadum).

10 mg100ug500 mg100ul25 mg100ug 5 G25 mg 5 G50 ug 100.00 ug1 g

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#32781208   2020/08/08 To Up

Pb exposure triggers MAPK-dependent inflammation by activating oxidative stress and miRNA-155 expression in carp head kidney.

Lead (Pb) is a toxic heavy metal and an aquatic pollutant. Various amounts of heavy metals are released into the environment through industrial discharge, causing excessive contamination of aquatic ecosystems. The head kidney is a unique immune organ of the bony fish and plays an important role in the metabolism of heavy metals. Studies of toxic Pb exposure that have investigated the head kidney of carp are limited. This study was carried out to explore the potential immunotoxicity effects of Pb and the specific related mechanisms in the carp head kidney. Pb poisoning was shown to induce the production of reactive oxygen species (ROS) and increase the expression levels of phosphorylated proteins related to the MAPK pathway, including p38, extracellular signal-regulated protein kinase (ERK), and c-Jun N-terminal kinase (JNK). We also found that microRNA-155 played a key role in regulating the production of inflammatory factors TNF-α, IL-1β, and IL-6, and the pre-miRNA-155 inhibitor reversed the Pb-induced inflammation. In conclusion, these in vitro and in vivo findings suggest that oxidative stress and the MAPKs are involved in the Pb-induced inflammasome response, and the production of microRNA-155 aggravated the occurrence of inflammation in carp head kidney.
Hongyuan Jing, Qirui Zhang, Shu Li, Xue-Jiao Gao

1691 related Products with: Pb exposure triggers MAPK-dependent inflammation by activating oxidative stress and miRNA-155 expression in carp head kidney.

4 Arrays/Slide8 Sample Kit16-22 Sample Kit4 Membranes/Box4 Sample Kit

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#32781170   2020/08/08 To Up

Hydroxytyrosol enhances cisplatin-induced ototoxicity: Possible relation to the alteration in the activity of JNK and AIF pathways.

Hydroxytyrosol (HT), a polyphenol widely contained as an ester in olive fruits and olive leaves, exhibits a broad spectrum of effectiveness. The present study was designed to investigate the effect of HT alone as well as in the combination with cisplatin on the House Ear Institute-Organ of Corti 1 cells (HEI-OC1) and C57BL/6 cochlear hair cells in vitro. The cell viability was measured by cell counting kit-8 (CCK8) assay. The levels of reactive oxygen species were evaluated by Dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining. The expression of phosphorylated Jun N-terminal kinase (p-JNK) and cleaved-caspase 3 was assessed by Western blotting. The apoptosis was detected by terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) staining. The distribution of apoptosis inducing factor (AIF) was determined by immunofluorescent staining. HT alleviated the levels of reactive oxygen species in both untreated state and after cisplatin stimulus. However, HT at concentration of 100 μM decreased the cell viability of HEI-OC1 from 100 ± 17.38% in control group to 50.17 ± 1.89% and increased the expression of p-JNK and c-caspase 3 from 0.62 ± 0.10, 0.20 ± 0.050 in the control group to 1.24 ± 0.18, 0.85 ± 0.18 in the group treated with 30 μM cisplatin, as well as to 1.64 ± 0.14, 1.44 ± 0.12 in the group with 30 μM cisplatin +100 μM HT, respectively. Meanwhile, HT triggered AIF transferring to nuclei and, also, led to cochlear HCs arranging disorderly and missing. Moreover, HT elevated the expression of p-JNK and c-caspase 3 from 1.00 ± 0.27, 1.00 ± 0.26 in the control group to 2.23 ± 0.24, 22.87 ± 3.80 in the group with 30 μM cisplatin, and to 2.75 ± 0.23, 31.56 ± 3.86 in the group with 30 μM cisplatin+100 μM HT correspondingly. Taken together, data from this work reveal that HT itself possesses toxic effect on HCs mainly thorough AIF-dependent apoptosis, while, it aggravates the ototoxicity-caused by cisplatin via both JNK and AIF pathways related apoptosis. Findings from this work offer clear evidence that that HT might not be recommended to utilize for preventing cisplatin-induced ototoxicity.
Weiwei Zhang, Rongjun Man, Xiaoyu Yu, Huiming Yang, Qianqian Yang, Jianfeng Li

1194 related Products with: Hydroxytyrosol enhances cisplatin-induced ototoxicity: Possible relation to the alteration in the activity of JNK and AIF pathways.

1 kit1 kit196 wells

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#32781144   2020/08/08 To Up

Sellar cholesterol granuloma mimicking cystic sellar lesions: A report of three cases and literature review.

Cystic lesions in the sellar region include a variety of entities as craniopharyngioma, Rathke's cleft cyst, intrasellar arachnoid cyst, cystic pituitary adenomas, cholesterol granulomas and xanthogranulomas The distinction among them remains a preoperative challenge due to similarities in their clinical and radiological findings.
Martin Pilonieta, Monica Martin, Juan M Revuelta Barbero, Douglas A Hardesty, Ricardo L Carrau, Bradley A Otto, Jose Otero, Luma Ghalib, Daniel M Prevedello

1249 related Products with: Sellar cholesterol granuloma mimicking cystic sellar lesions: A report of three cases and literature review.

0.1 mg

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#32781015   2020/08/08 To Up

Exosomes in head and neck cancer: Roles, mechanisms and applications.

Exosomes are extracellular vesicles with a diameter of 30-100 nm that exist stably in various body fluids, including the blood, cerebrospinal fluid, urine, saliva, and serous cavity effusion. Compared with other vesicles, exosomes have a unique double-layer saclike structure and an endosomal origin. Depending on the physiological state of donor cells, there is heterogeneity in the bioactive substances present in exosomes, including proteins, RNA, DNA, lipids, and metabolites, providing a rich source of potential biomarkers for disease diagnosis. In head and neck cancer (HNC), exosomes have applications in disease diagnosis and prognosis based on liquid biopsy. Nonetheless, the functions and mechanisms of exosomes in HNC still remain unclear. Recent studies have elucidated the substances and signals transmitted between cancer cells using exosomes and have discussed tumor occurrence, development, metastasis, immune regulation, and drug resistance regulated by exosomes in HNC. In this review, we discuss these recent findings and highlight the significant role of exosomes in HNC, with a focus on the mechanism of tumor progression and the potential clinical value of exosomes as biomarkers and therapeutic targets.
Jun Cao, Mengdi Zhang, Feng Xie, Jianlin Lou, Xiaoxue Zhou, Long Zhang, Meiyu Fang, Fangfang Zhou

2299 related Products with: Exosomes in head and neck cancer: Roles, mechanisms and applications.

5mgEach

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#32781011   2020/08/08 To Up

The danish head and neck cancer group (DAHANCA) 2020 radiotherapy guidelines.


Kenneth Jensen, Jeppe Friborg, Christian Rønn Hansen, Eva Samsøe, Jørgen Johansen, Maria Andersen, Bob Smulders, Elo Andersen, Martin Skovmos Nielsen, Jesper Grau Eriksen, Jørgen Breede Baltzer Petersen, Ulrik Vindelev Elstrøm, Anne Ivalu Holm, Mohammed Farhadi, Morten Hjartdal Morthorst, Peter Sandegaard Skyt, Jens Overgaard, Cai Grau

1727 related Products with: The danish head and neck cancer group (DAHANCA) 2020 radiotherapy guidelines.

EachEach

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#32780966   2020/08/11 To Up

Changes in steering performance and muscle activity of the upper extremity according to the position of the spinner knob.

A spinner knob attached to the steering wheel enables one-handed driving. However, changes in steering performance and muscle activity of the upper extremity based on the differences in the position of the knob are unknown.
Rikiya Shirato, Riho Imaizumi, Shiori Oi, Shiho Mishina

2904 related Products with: Changes in steering performance and muscle activity of the upper extremity according to the position of the spinner knob.

1500 Units1

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#32780886   2020/08/11 To Up

Does Nasal Secretion Decrease in Sjögren Syndrome and Does This Affect Nasal Function?

Sjögren syndrome is a systemic inflammatory disease causing gland dysfunction. Few (and contradictory) reports on the mucosal effects of Sjögren syndrome have appeared. Here, we objectively demonstrate nasal dryness in Sjögren syndrome patients and explore the effect of such dryness on olfaction.
Erdem Eren, Koray Balcı, Önay Gerçik, Gökhan Kabadayı, Servet Akar

2565 related Products with: Does Nasal Secretion Decrease in Sjögren Syndrome and Does This Affect Nasal Function?

100 μg96 assays1mg100ug Lyophilized100ug1 g100ug Lyophilized 100 G1 Set16 Arrays/Slide

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