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#32452745   2020/05/22 To Up

Implementation of Universal Tumor Screening of Colorectal Cancer for Detection of Lynch Syndrome at a Hispanic-Rich County Hospital.

In 2014, a reflexive screening protocol for Lynch syndrome (LS) via an immunohistochemistry (IHC) assay was shown to be cost-effective; however, the screening rates at a predominant Hispanic-rich institution are unclear. We hypothesized that implementation of a universal tumor screening (UTS) protocol requiring screening for LS via IHC in patients with newly diagnosed colorectal cancer (CRC) at our Hispanic-rich institution would improve detection of LS by increasing screening rates.
Tyler W Snedden, Andrew McCracken, Anusha Vaidyanathan, Anna Taranova, Roberto Villarreal, Samina Qamar, Sukeshi Patel Arora

2341 related Products with: Implementation of Universal Tumor Screening of Colorectal Cancer for Detection of Lynch Syndrome at a Hispanic-Rich County Hospital.

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#32452400   2020/04/27 To Up

Region-specific changes in the mRNA and protein expression of LCPUFA biosynthesis enzymes and transporters in the placentae of women with preeclampsia.

The biosynthesis and transport of long chain polyunsaturated fatty acids (LCPUFA) require the activity of fatty acid desaturase (FADS) enzymes, fatty acid transport proteins (FATP) and fatty acid binding proteins (FABP). In a previous study we have demonstrated region-specific changes in the LCPUFA levels in preeclampsia (PE) as compared to the normotensive control (NC) placentae.
Alka Rani, Preeti Chavan-Gautam, Savita Mehendale, Girija Wagh, Narayanan Subramaniam Mani, Sadhana Joshi

1933 related Products with: Region-specific changes in the mRNA and protein expression of LCPUFA biosynthesis enzymes and transporters in the placentae of women with preeclampsia.

100 μg100 μg100μg2 Pieces/Box100 μg100 μg1

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#32450530   2020/05/22 To Up

Imperatorin suppresses IL-1β-induced iNOS expression via inhibiting ERK-MAPK/AP1 signaling in primary human OA chondrocytes.

Joint inflammation is a key player in the pathogenesis of osteoarthritis (OA). Imperatorin, a plant-derived small molecule has been reported to have anti-inflammatory properties; however, its effect on chondrocytes is not known. Here, we investigated the effects of Imperatorin on interleukin-1β (IL-1β) induced expression of inducible nitric oxide synthase (iNOS) and nitric oxide production in primary human OA chondrocytes and cartilage explants culture under pathological conditions and explored the associated signaling pathways. We pretreated chondrocytes or explants with Imperatorin (50 μM) followed by IL-1β (1 ng/ml), and the culture supernatant was used to determine the levels of nitrite production by Griess assay and chondrocytes were harvested to prepare cell lysate or RNA for gene expression analysis of iNOS by Western blot or qPCR and in explants by immunohistochemistry (IHC). Pretreatment of primary chondrocytes and cartilage explants with Imperatorin suppressed IL-1β induced expression of iNOS and NO production. Imperatorin blocked the IL-1β-induced phosphorylation of ERK-MAPK/AP1 signaling pathway to suppress iNOS expression. The role of ERK in the regulation of iNOS expression was verified by using ERK inhibitor. Interestingly, we also found that Imperatorin binds to iNOS protein and inhibits its activity in vitro. Our data demonstrated that Imperatorin possess strong anti-inflammatory activity and may be developed as a therapeutic agent for the management of OA.
Nashrah Ahmad, Mohammad Y Ansari, Shabana Bano, Tariq M Haqqi

1245 related Products with: Imperatorin suppresses IL-1β-induced iNOS expression via inhibiting ERK-MAPK/AP1 signaling in primary human OA chondrocytes.

100 ul2 Pieces/Box50 ul5ug 100ul100 μg96 wells (1 kit)100 μg2 100μg/vial100 μg100 μg

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#32450150   2020/05/22 To Up

Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody.

Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF.
Levente Szeredi, Erika Bakcsa, Zoltán Zádori, István Mészáros, Ferenc Olasz, Ádám Bálint, Gabriella Locsmándi, Károly Erdélyi

2304 related Products with: Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100

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#32449239   2020/05/24 To Up

Peptide vaccine as an adjuvant therapy for glypican-3 positive hepatocellular carcinoma induces peptide specific CTLs and improves long prognosis.

There is no established post-operative adjuvant therapy for hepatocellular carcinoma (HCC), and improvement of patient prognosis has been limited. We conducted long-term monitoring of patients within a phase II trial that targeted a cancer antigen, glypican-3 (GPC3), specifically expressed in HCC. We sought to determine if the GPC3 peptide vaccine is an effective adjuvant therapy by monitoring disease-free survival and overall survival. We also tracked GPC3 immunohistochemical (IHC) staining, cytotoxic T lymphocyte (CTL) induction, and post-operative plasma GPC3 for a patient group that was administered the vaccine (n = 35) and an unvaccinated group that only had surgery (n = 33). The 1-year recurrence rate after surgery was reduced by approximately 15%, and the 5- and 8-year survival rates were improved by approximately 10% and 30%, respectively, in the vaccinated group compared to the unvaccinated group. Patients who were positive for GPC3 IHC staining were more likely to have induced CTLs, and 60% survived beyond 5 years. Vaccine efficacy had a positive relationship with plasma concentration of GPC3; high concentrations increased the 5-year survival rate to 75%. We thus expect GPC3 vaccination in patients with HCC, who are positive for GPC3 IHC staining and/or plasma GPC3 to induce CTL and significantly improved long-term prognosis.
Masatake Taniguchi, Shoichi Mizuno, Toshiaki Yoshikawa, Norihiro Fujinami, Motokazu Sugimoto, Shin Kobayashi, Shinichiro Takahashi, Masaru Konishi, Naoto Gotohda, Tetsuya Nakatsura

2846 related Products with: Peptide vaccine as an adjuvant therapy for glypican-3 positive hepatocellular carcinoma induces peptide specific CTLs and improves long prognosis.

1 mg200ul1 mg20 100ug 0.1 mg 50 ug100ug Lyophilized100ug100ug Lyophilized250 TESTS1 mg

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