Only in Titles

Search results for: kits

paperclip

Error loading info... Pleas try again later.
paperclip

#32470451   2020/05/26 To Up

Bcl2 inhibitor ABT737 reverses the Warburg effect via the Sirt3-HIF1α axis to promote oxidative stress-induced apoptosis in ovarian cancer cells.

Compared to normal cells, tumor cells maintain higher concentrations of reactive oxygen species (ROS) to support proliferation, invasion, and metastasis. Chemotherapeutic drugs often induce tumor cell apoptosis by increasing intracellular ROS concentrations to highly toxic levels. ABT737, which inhibits the apoptosis regulator B cell lymphoma 2 (Bcl2), increases the sensitivity of ovarian cancer cells to chemotherapeutic drugs by regulating the glucose metabolism, but the underlying mechanisms remain unclear. Therefore, we aimed to determine whether ABT737 promoted HO-induced tumor cell apoptosis by reversing glycolysis in ovarian cancer cells.
Delu Dong, Yuan Dong, Jiaying Fu, Shengyao Lu, Chunli Yuan, Meihui Xia, Liankun Sun

2308 related Products with: Bcl2 inhibitor ABT737 reverses the Warburg effect via the Sirt3-HIF1α axis to promote oxidative stress-induced apoptosis in ovarian cancer cells.

1

Related Pathways

paperclip

#32470200   2020/05/29 To Up

Penfluridol Triggers Mitochondrial-mediated Apoptosis and Suppresses Glycolysis in Colorectal Cancer Cells through Down-regulating Hexokinase-2.

Penfluridol, a commonly used antipsychotic agent in a clinical setting, exhibits potential anti-cancer properties against various human malignancies. Here, we investigated the effect of penfluridol on the biological behavior of CRC cells.
Ning-Ning Wang, Peng-Zhen Zhang, Jing Zhang, Hai-Ning Wang, Ling Li, Feng Ren, Peng-Fei Dai, Hui Li, Xiao-Feng Lv

2090 related Products with: Penfluridol Triggers Mitochondrial-mediated Apoptosis and Suppresses Glycolysis in Colorectal Cancer Cells through Down-regulating Hexokinase-2.

2 Pieces/Box2 Pieces/Box4 Membranes/Box

Related Pathways

paperclip

#32468054   2020/05/22 To Up

Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4.

The expression levels of microRNA (miR)‑340‑5p are reportedly decreased in the peripheral blood during acute ischemic stroke; however, the direct effect and mechanism of action of miR‑340‑5p in ischemic stroke remains largely unknown. The present study aimed to investigate the effects of miR‑340‑5p, and its mechanism of action, on PC12 cells following oxygen‑glucose deprivation/reperfusion (OGD/R) induction. OGD/R‑induced PC12 cells served as the cellular model and subsequently, mRNA expression levels of miR‑340‑5p and neuronal differentiation 4 (Neurod4) were analyzed using reverse transcription‑quantitative PCR. Tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6 expression levels were detected using ELISA kits, and flow cytometry was used to determine the rate of cellular apoptosis. In addition, a nitric oxide (NO) synthase activity assay kit was used to detect NO levels and a NADPH assay kit was used to measure NADPH levels. Western blotting was also performed to analyze protein expression levels of bax, bcl‑2, cleaved caspase 3 and phosphorylated endothelial NOS (eNOS), and the target gene of miR‑340‑5p was predicted using TargetScan software and verified using a dual‑luciferase reporter assay. The expression levels of miR‑340‑5p were decreased in PC12 cells following OGD/R induction and Neurod4 was identified as a target gene of miR‑340‑5p. In addition, miR‑340‑5p overexpression reduced inflammation, apoptotic rate, NO production and NADPH levels, in addition to increasing eNOS expression in PC12 cells following OGD/R induction. Notably, the overexpression of Neurod4 reversed the aforementioned effects of miR‑340‑5p on PC12 cells following OGD/R induction. In conclusion, the findings of the present study suggested that miR‑340‑5p may protect PC12 cells against OGD/R through targeting Neurod4, which could provide important implications for the treatment of ischemia‑reperfusion injury based on miR‑340‑5p expression levels in vivo.
Juan Wang, Ganzhe Liu

2080 related Products with: Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4.

96 assays5 x 50 ug10 rxns100 μg1x10e7 cells10 ug1.00 flask2 Pieces/Box50 ug

Related Pathways

paperclip

#32468008   2020/05/20 To Up

Effects of dihydroartemisinin on the gut microbiome of mice.

Dihydroartemisinin (DHA) is a semisynthetic derivative of artemisinin, which has been found to exhibit a broad range of biological activities, excluding antimalarial effects; however its effects on the gut microbiota remain poorly understood. The present study aimed to investigate the effects of DHA on the gut microbiome in mice and to determine its potential biological and pharmaceutical activities through its alteration of the gut microbiota. Serum glucose, triglyceride (TG), total cholesterol, lipopolysaccharide, high density lipoprotein‑cholesterol, low density lipoprotein‑cholesterol, alanine aminotransferase and aspartate aminotransferase levels in mice treated with DHA were analyzed using the corresponding detection kits. In addition, hematoxylin and eosin staining was performed to determine the pathological effects of DHA on the liver, kidney and intestinal tissues of mice, and the effects of DHA on the gut microbiome were analyzed using 16S ribosomal (r)DNA gene analysis. The results demonstrated that the TG serum levels of mice treated with DHA were significantly decreased compared with the control group. Furthermore, 16S rDNA gene analysis demonstrated that the bacterial diversity of mice treated with DHA was enriched compared with the control group. The DHA group exhibited increased numbers of Firmicutes and Saccharibacteria, and decreased Deferribacteres and Actinobacteria compared with the control group at the phylum level. Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment analysis also revealed that the signaling pathways associated with 'Energy metabolism' and 'Nucleotide metabolism' were upregulated, whereas the signaling pathways associated with 'Infectious diseases and 'Neurodegenerative diseases' were downregulated in the DHA group compared with the control group. In conclusion, the findings of the present study indicated that DHA may significantly decrease the serum TG levels and alter the gut microbiota, which suggested its potential to be used for the treatment of hyperlipidemia, inflammatory and neurodegenerative disorders.
Yanyan Liu, Yanhong Yang, Yuting Lei, Lanxiang Yang, Xueying Zhang, Jian Yuan, Zili Lei

1044 related Products with: Effects of dihydroartemisinin on the gut microbiome of mice.

5 G1 g1mg100 U 25 ml 100.00 ul100 1 ml 100ul200 units

Related Pathways

paperclip

#32467909   2019/06/21 To Up

A revision of the genus with a focus on subsection .

Specimens belonging to taxa traditionally assigned to the subsection of the genus were analyzed both morphologically and molecularly. Samples included mainly European collections, selected GenBank accessions, and specimens of various North American taxa described by Smith (1972) and deposited at the Herbarium of the University of Michigan (MICH). Three additional taxa from Africa and Central America were also included. Bayesian and Maximum Likelihood analyses of two loci (ITS and ) independently and together supported the monophyletic nature of the subsection , and identified nine statistically supported clades within the subsection. North American and European species often fell within the same clade, suggesting a relatively recent origin of the subsection or human induced intercontinental movement. While this study determines for the first time that the presence of a white veil is diagnostic for the entire subsection, very few morphological traits were associated with individual clades, but clades were often distinctively different in terms of habitat association, suggesting that trophic interactions may have driven the evolution of this group of fungi. Combined, morphological and DNA analyses revealed both expected and unexpected synonymies. The new combinations and are proposed, and the new species is described. New information is provided on the taxonomic status and distribution of several species including , , , , , , . , , , , , , Kits van Waveren, . In total, 13 synonymies were proposed. Based on DNA data, five species of uncertain validity were confirmed as valid, while six species may be ambiguous and may require an in-depth re-analysis. The information gathered in this study was used to generate a key to the species of the subsection .
P Voto, F Dovana, M Garbelotto

2330 related Products with: A revision of the genus with a focus on subsection .

100.00 ug100.00 ul1 ml 100ul100.00 ul

Related Pathways

paperclip

#32467748   2020/05/15 To Up

Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome.

Specific anti-phospholipids antibodies (aPLs) are used as classification criteria of the antiphospholipid syndrome (APS). These aPLs, although essential for diagnosis, do not predict disease phenotypes, which may require specific therapies. Non-criteria aPLs are rarely evaluated and their role is yet to be defined. In the current study, we aimed to examine the association between criteria and non-criteria aPLs and APS phenotypes.
Ilan Volkov, Luciana Seguro, Elaine P Leon, László Kovács, Dirk Roggenbuck, Peter Schierack, Boris Gilburd, Andrea Doria, Maria G Tektonidou, Nancy Agmon-Levin

1620 related Products with: Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome.

1 ml

Related Pathways

paperclip

#32467672   2020/05/19 To Up

Proteome-wide identification of arginine methylation in colorectal cancer tissues from patients.

Protein arginine methylation reaction is catalyzed by protein arginine methyltransferase (PRMT) and the modification is implicated in various diseases including cancer. Currently, thousands of arginine methylation sites have been identified using high-resolution mass spectrometry-based proteomics technology. However, identification of arginine methylation using clinical samples at proteome level has not been reported yet. The objective of the present study was to identify, monomethyl-arginine (MMA) and asymmetric dimethyl-arginine (ADMA) sites in colorectal cancer (CRC) tissues at proteome level.
Yongchul Lim, Ju Yeon Lee, Su Jin Ha, Suyeun Yu, Jung Kyong Shin, Hee Cheol Kim

2963 related Products with: Proteome-wide identification of arginine methylation in colorectal cancer tissues from patients.



Related Pathways

paperclip

#32466458   2020/05/26 To Up

Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2.

The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19.
Nadin Younes, Duaa W Al-Sadeq, Hadeel Al-Jighefee, Salma Younes, Ola Al-Jamal, Hanin I Daas, Hadi M Yassine, Gheyath K Nasrallah

1468 related Products with: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2.

100 ug/vial200 100 µg100 1roll1mg100 100 µg1

Related Pathways

paperclip

#32466124   2020/05/25 To Up

Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study.

The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (Δdecayed, missing, and filled teeth (ΔDMFT) score increased during the 3-year period) and non-increased group (ΔDMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group ( = 14) was similar to that in the non-increased group ( = 41). Relative abundances of the family ( = 0.007) and genera ( = 0.007) and ( = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.
Yoko Uchida-Fukuhara, Daisuke Ekuni, Md Monirul Islam, Kota Kataoka, Ayano Taniguchi-Tabata, Daiki Fukuhara, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Nanami Sawada, Yoshiaki Iwasaki, Manabu Morita

2443 related Products with: Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study.

0.1 mg10 mg100ul500 mg50 ug 25 mg100tests 5 G100ug25 mg 5 G96 wells (1 kit)

Related Pathways