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#35961784   2022/08/12 To Up

TMPRSS3 expression is limited in spiral ganglion neurons: implication for successful cochlear implantation.

It is well established that biallelic mutations in transmembrane protease, serine 3 () cause hearing loss. Currently, there is controversy regarding the audiological outcomes after cochlear implantation (CI) for -associated hearing loss. This controversy creates confusion among healthcare providers regarding the best treatment options for individuals with -related hearing loss.
Yuan-Siao Chen, Ernesto Cabrera, Brady J Tucker, Timothy J Shin, Jasmine V Moawad, Douglas J Totten, Kevin T Booth, Rick F Nelson

1246 related Products with: TMPRSS3 expression is limited in spiral ganglion neurons: implication for successful cochlear implantation.

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#35961777   2022/08/12 To Up

-lineage-independent origin of dermal lymphatics shown by temporally restricted lineage tracing.

The developmental origins of lymphatic endothelial cells (LECs) have been under intense research after a century-long debate. Although previously thought to be of solely venous endothelial origin, additional sources of LECs were recently identified in multiple tissues in mice. Here, we investigated the regional differences in the origin(s) of the dermal lymphatic vasculature by lineage tracing using the pan-endothelial line. Tamoxifen-induced labeling of blood ECs at E9.5, before initiation of lymphatic development, traced most of the dermal LECs but with lower efficiency in the lumbar compared with the cervical skin. By contrast, when used at E9.5 but not at E11.5, 4-hydroxytamoxifen, the active metabolite of tamoxifen that provides a tighter window of Cre activity, revealed low labeling frequency of LECs, and lymphvasculogenic clusters in the lumbar skin in particular. Temporally restricted lineage tracing thus reveals contribution of LECs of -lineage-independent origin to dermal lymphatic vasculature. Our results further highlight Cre induction strategy as a critical parameter in defining the temporal window for stage-specific lineage tracing during early developmental stages of rapid tissue differentiation.
Yan Zhang, Henrik Ortsäter, Ines Martinez-Corral, Taija Mäkinen

1579 related Products with: -lineage-independent origin of dermal lymphatics shown by temporally restricted lineage tracing.

100 50 UG50ul (1mg/ml)1.00 flask 25 G1.00 kit50ul (1mg/ml)

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#35961772   2022/08/10 To Up

Lineage analysis of -expressing cells in the developing midbrain suggests that progressive competence restriction in dopaminergic progenitor cells contributes to the establishment of dopaminergic neuronal diversity.

Midbrain dopaminergic (mDA) neurons are generated from a ventral midbrain progenitor zone over a time span of several days (embryonic day (E)10.0-E14.5 in mouse). Within this neurogenic period, a progressively changing fate potential of mDA progenitors could contribute to the generation of diverse mDA neuronal subpopulations. To test this idea, we combined inducible genetic fate mapping and intersectional labeling approaches to trace the lineage of cells expressing the chemokine receptor CXCR4. The transcript is expressed in mDA progenitors and precursors but not in differentiated mDA neurons. -expressing mDA progenitors/precursors labeled at E11.5 develop into a broad range of mDA neurons, whereas labeling of the -lineage at later time points (E12.5-E15.5) results in an increasingly restricted contribution to mDA neurons proceeding from lateral to medial in the substantia nigra and from dorsal to ventral in the ventral tegmental area. In parallel, the innervation of dopaminergic projection targets by mDA neurons derived from -expressing cells is becoming more restricted: the late-generated mDA neurons innervate only the medial-rostral regions in the dorsal striatum and only the medial shell in the nucleus accumbens. Our results suggest that mDA progenitor cells become increasingly restricted in their cell fate potential over time.Midbrain dopaminergic (mDA) neurons modulate cognitive processes, voluntary movement and reward behavior. The degeneration of a subset of mDA neurons results in the motor deficits in Parkinson's disease while altered dopamine transmission is associated with neuropsychiatric disorders including depression and schizophrenia. The dopaminergic system is composed of molecularly distinct subpopulations and discrete dopaminergic circuits which influence distinct aspects of behavior. Understanding how diversity in the dopaminergic system is established is essential for a better insight into its functional output and the mechanisms underlying its dysfunction. Our study shows that mDA progenitors change their competence over time to generate at different developmental time points mDA subtypes that are distinct in their anatomical location and projection targets.
Alessandro Petese, Franca L Fries, Bianca Broske, Ralf Stumm, Sandra Blaess

1480 related Products with: Lineage analysis of -expressing cells in the developing midbrain suggests that progressive competence restriction in dopaminergic progenitor cells contributes to the establishment of dopaminergic neuronal diversity.