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#32473611   2020/05/30 To Up

Clonal lineage from normal endometrium to ovarian clear cell carcinoma through ovarian endometriosis.

Clear cell carcinoma of the ovary is thought to arise from endometriosis. Additionally, retrograde menstruation of shed endometrium is considered the origin of endometriosis. However, little evidence supports cellular continuity from uterine endometrium to clear cell carcinoma through endometriosis at the genomic level. Here, we performed multiregional whole-exome sequencing to clarify clonal relationships among uterine endometrium, ovarian endometriosis, and ovarian clear cell carcinoma in a 56-year-old patient. Many somatic mutations including cancer-associated gene mutations in ARID1A, ATM, CDH4, NRAS, and PIK3CA were shared among epithelium samples from uterine endometrium, endometriotic lesions distant from and adjacent to the carcinoma, and the carcinoma. The mutant allele frequencies of shared mutations increased from uterine endometrium to distant endometriosis, adjacent endometriosis, and carcinoma. Although a splice site mutation of ARID1A was shared among the four epithelium samples, a frameshift insertion in ARID1A was shared by adjacent endometriosis and carcinoma samples, suggesting that the biallelic mutations triggered malignant transformation. Somatic copy number alterations, including loss of heterozygosity events at PIK3CA and ATM, were identified only in adjacent endometriosis and carcinoma, suggesting that mutant allele-specific imbalance is another key factor driving malignant transformation. By reconstructing a clonal evolution tree based on the somatic mutations, we showed that the epithelium samples were derived from a single ancestral clone. Although the study was limited in a single patient, the results from this illustrative case could suggest the possibility that epithelial cells of ovarian endometriosis and clear cell carcinoma were descendants of uterine endometrial epithelium.
Kazuaki Suda, Luis Antonio Cruz Diaz, Kosuke Yoshihara, Hirofumi Nakaoka, Nozomi Yachida, Teiichi Motoyama, Ituro Inoue, Takayuki Enomoto

1964 related Products with: Clonal lineage from normal endometrium to ovarian clear cell carcinoma through ovarian endometriosis.



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#32473607   2020/05/30 To Up

Acute Liver Injury in COVID-19: Prevalence and Association with Clinical Outcomes in a Large US Cohort.

Coronavirus disease 2019 (COVID-19) has been associated with acute liver injury manifested by increased liver enzymes in reports worldwide. Prevalence of liver injury and associated clinical characteristics are not well-defined. We aim to identify the prevalence of and risk factors for development of COVID-19 associated acute liver injury in a large cohort in the United States.
Meaghan M Phipps, Luis H Barraza, Elijah D LaSota, Magdalena E Sobieszczyk, Marcus R Pereira, Elizabeth X Zheng, Alyson N Fox, Jason Zucker, Elizabeth C Verna

2420 related Products with: Acute Liver Injury in COVID-19: Prevalence and Association with Clinical Outcomes in a Large US Cohort.



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#32473601   2020/05/30 To Up

Biological Evaluation of Keggin-type Polyoxometalates on Tyrosinase: Kinetics and Molecular Modeling.

Abnormal overexpression of tyrosinase activity can lead to the production of hyperpigmentation in human skin and enzymatic browning in fruits and vegetables. Herein, the inhibition and mechanism of the H PMo O and two transition metal-substituted Keggin-type polyoxometalates (Na PMo CoO and Na PMo ZnO ) on tyrosinase were studied by kinetics and molecular modeling. Kinetic studies indicated that all compounds had more potent inhibitory activities than standard arbutin, and H PMo O (IC = 0.443 ± 0.006 mM) is approximately 15-fold stronger inhibition than arbutin. Additionally, all compounds inhibited tyrosinase in a reversible competitive manner. Intriguingly, molecular modeling elucidated that three compounds competitively bind to tyrosinase mainly through more interactions with Cu ions and the amino acid residue capable of forming cation-π and hydrogen bonding, forming a reversible non-covalent complex. Molecular simulation study correlated well with the biological activity of three compounds in vitro. This work provided new insights on design and synthesis of polyoxometalates as tyrosinase inhibitors in the field of medicine, cosmetic and food.
Guoxiang Chi, Lefang Xie, Meijuan Zhao, Li Wang, Fang Wang, Jian Li, Aping Zheng

2774 related Products with: Biological Evaluation of Keggin-type Polyoxometalates on Tyrosinase: Kinetics and Molecular Modeling.

96T10 ug50 ug100200ul 6 ml Ready-to-use 20

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#32473559   2020/05/12 To Up

Cyanine-based fluorescent indicator for mercury ion and bioimaging application in living cells.

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Guangjin Li, Yihan Guan, Fengying Ye, Sheng Hua Liu, Jun Yin

1240 related Products with: Cyanine-based fluorescent indicator for mercury ion and bioimaging application in living cells.

1x10e7 cells100ug Lyophilized1.00 flask1mg0.1ml 100ul0.25 mg

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#32473549   2020/05/12 To Up

Association between gastrin-releasing peptide receptor expression as assessed with [Ga]Ga-RM2 PET/CT and histopathological tumor regression after neoadjuvant chemotherapy in primary breast cancer.

The gastrin-releasing peptide receptor is overexpressed in breast cancer (BC) tissue and can be visualized by positron emission tomography (PET) using the GRPR antagonist [Ga]Ga-RM2. This study assessed tumor binding of RM2 before and after neoadjuvant chemotherapy (NAC) in primary BC with reference to residual tumor size in the resected specimen.
Kerstin Michalski, Christian Stoykow, Peter Bronsert, Ingolf Juhasz-Böss, Philipp T Meyer, Juri Ruf, Thalia Erbes, Jasmin Asberger

2506 related Products with: Association between gastrin-releasing peptide receptor expression as assessed with [Ga]Ga-RM2 PET/CT and histopathological tumor regression after neoadjuvant chemotherapy in primary breast cancer.



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#32473517   2020/05/27 To Up

MRI-guided voxel-based automatic semi-quantification of dopamine transporter imaging.

Functional imaging with I-FP-CIT SPECT suffers from poor spatial resolution resulting in partial-volume effect, which affects the subsequent semi-quantification. Definition of regions of interest for semi-quantification is further subject to user's experience and inter-observer variability. The aim of this work has been to develop an automatic method for definition of volumes of interest and partial-volume correction using patient-specific MRI and providing complete contrast recovery in striatal region.
Jiri Trnka, Petr Dusek, Martin Samal, Karel Kupka, Karel Sonka, Evzen Ruzicka

1613 related Products with: MRI-guided voxel-based automatic semi-quantification of dopamine transporter imaging.

0.1 ml100 μg100 μg100ug0.1 ml0.1 ml100ugOne 96-Well Microplate Ki 1 kit(s)

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#32473441   2020/05/07 To Up

Using the Google Earth Engine to estimate a 10 m resolution monthly inventory of soil fugitive dust emissions in Beijing, China.

Soil fugitive dust (SFD) is an important contributor to ambient particulate matter (PM), but most current SFD emission inventories are updated slowly or have low resolution. In areas where vegetation coverage and climatic conditions undergo significant seasonal changes, the classic wind erosion equation (WEQ) tends to underestimate SFD emissions, increasing the need for higher spatiotemporal data resolution. Continuous acquisition of precise bare soil maps is the key barrier to compiling monthly high-resolution SFD emission inventories. In this study, we proposed taking advantage of the massive Landsat and Sentinel-2 imagery data sets stored in the Google Earth Engine (GEE) cloud platform to enable the rapid production of bare soil maps with spatial resolutions of up to 10 m. The resulting improved spatiotemporal resolution of wind erosion parameters allowed us to estimate SFD emissions in Beijing as being ~5-7 times the level calculated by the WEQ. Spring and winter accounted for >85% of SFD emissions, while April was the dustiest month with SFD emissions of PM exceeding 11,000 t. Our results highlighted the role of SFD in air pollution during winter and spring in northern China, and suggested that GEE should be further used for image acquisition, data processing, and compilation of gridded SFD inventories. These inventories can help identify the location and intensity of SFD sources while providing supporting information for local authorities working to develop targeted mitigation measures.
Aobo Liu, Qizhong Wu, Xiao Cheng

1477 related Products with: Using the Google Earth Engine to estimate a 10 m resolution monthly inventory of soil fugitive dust emissions in Beijing, China.

1 kit1 kit1 Set4 Membranes/Box1 module

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#32473401   2020/05/07 To Up

Tumor grade-related language and control network reorganization in patients with left cerebral glioma.

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Binke Yuan, Nan Zhang, Jing Yan, Jingliang Cheng, Junfeng Lu, Jinsong Wu

2506 related Products with: Tumor grade-related language and control network reorganization in patients with left cerebral glioma.



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#32473392   2020/05/27 To Up

Miliusins; cytotoxic neolignans from the leaves of Miliusa sessilis.

Eight undescribed neolignans and an undescribed propanoid dimer were isolated from the leaves of Miliusa sessilis, together with two known compounds, dehydrodieugenol A and dehydrodieugenol B. All structures were elucidated by extensive spectroscopic data analysis and the structure of (7S,8R)-5'-hydroxy-3,4-dimethoxy-4',7-epoxy-8,3'-neolign-8'-en-9-acetate (miliusin A) was further confirmed by X-ray crystallographic analysis. The absolute configurations were determined using circular dichroism (CD) data analysis and the modified Mosher's method. All isolated compounds were evaluated for their cytotoxic activities against four human cancer cell lines (HeLa, HN22, HepG2, and HCT116), including one normal-type cell line (HaCaT) using MTT assay. (7S,8R)-5'-hydroxy-3,4-dimethoxy-4',7-epoxy-8,3'-neolign-8'-en-9-ol (miliusin B) was found to exhibit the most promising cytotoxic effect against Hela cells with the lowest IC value of 0.04 μM and the highest selective index of 187.8, highlighting miliusin B as an attractive candidate for cervical cancer drug development.
Yupa Pootaeng-On, Purin Charoensuksai, Pawaris Wongprayoon, Suwadee Jiajaroen, Kittipong Chainok, Kanok-On Rayanil

1390 related Products with: Miliusins; cytotoxic neolignans from the leaves of Miliusa sessilis.

2000 IU1 mg100.00 ul500 Units1 kit110400 assays

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#32473368   2020/05/27 To Up

Dingkun pill replenishes diminished ovarian reserve through protection of primordial follicle pool by modulating the PI3K/AKT/mTOR signaling pathway.

Diminished ovarian reserve (DOR) can lead to poor fertility and shorten the reproductive lifespan of female. The Dingkun Pill (DKP), a traditional Chinese-patented medication has been an integral part of Chinese treatment for centuries for the management of gynecological diseases. Relevant clinical studies have shown that DKP is able to protect against DOR, however, its mechanism of action is not yet fully known.
Kun Ma, Yanxia Chen, Xiaodi Fan, Yuan Yuan, Kaili Wang, Caidie Tian, Min Li

1483 related Products with: Dingkun pill replenishes diminished ovarian reserve through protection of primordial follicle pool by modulating the PI3K/AKT/mTOR signaling pathway.

2 Pieces/Box1.5 x 10^6 cells5mg2 Pieces/Box5mgInhibitors2 Pieces/Box2 Pieces/Box5mg2 Pieces/Box1.5x10(6) cells50mg

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