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#33079366   // To Up

Treatment of Necrotizing Soft Tissue Infections: IVIG.

Immunoglobulins are key effector molecules in the humoral immune response. Intravenous polyspecific immunoglobulin (IVIG) is a preparation of polyclonal serum immunoglobulins, typically IgG, from thousands of donors. It has been used as adjunctive therapy in critically ill patients with severe infections, i.e. sepsis, septic shock, and necrotizing soft tissue infections. IVIG has been used for patients with severe invasive group A streptococcal infection since the early nineties and off-label use of IVIG for necrotizing soft tissue infections is common. It is also used for a variety of autoimmune, inflammatory, and immunodeficiency diseases. A meta-analysis of the clinical studies available for IVIG use in group A streptococcal toxic shock syndrome indicates a survival benefit. A blinded, placebo-controlled clinical trial (INSTINCT) assessed the effect of IVIG in 100 intensive care unit patients with necrotizing soft tissue infections, including all bacterial etiologies. The study did not demonstrate any effect on self-reported physical functioning at 6 months. In this chapter, we review the mechanisms of action of IVIG and the clinical studies that are available for necrotizing soft tissue infections as well as severe group A streptococcal infections.
Martin Bruun Madsen, Helena Bergsten, Anna Norrby-Teglund

1970 related Products with: Treatment of Necrotizing Soft Tissue Infections: IVIG.

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#33078915   2021/02/01 To Up

Anti-fibrotic effect of intravenous umbilical cord-derived mesenchymal stem cells (UC-MSCs) injection in experimental rats induced liver fibrosis.

Aim To investigate the effect of umbilical cord-derived mesenchymal stem cells (UC-MSCs) administration among liver fibrosis experimental rat model via the regulation of angiotensin II type 1 receptor (AT1R) and platelet-derived growth factor-β (PDGF-β) due to their therapeutic potential to replace liver transplantation for advanced liver fibrosis. Yet the mechanism of action has been questionably associated with UC-MSCs fibrosis regression properties. Methods Sprague-Dawley (SD) rats (n=18) were separated into three groups (control, untreated liver fibrosis, and UC-MSCs treated group). Serum PDGF-β level was determined by enzymelinked immunosorbent assay (ELISA) following 14 days of UCMSCs injection. Meanwhile, AT1R expression was interpreted based on immunoreactive score (IRS) stained using polyclonal antibody and liver fibrosis stained with hematoxylin & eosin was graded using the METAVIR score. Results UC-MSCs were isolated successfully from rat umbilical cord. Liver fibrosis was observed following 14 weeks of CCl4 injection concurrent with higher serum level of PDGF-β, but the UC-MSCs-treated group had lower level (980.08 ±289.41 and 606.42±109.85 for untreated liver fibrosis and UC-MSCs treated group, respectively; p=0.004). There was also a high expression of AT1R among untreated liver fibrosis group, as well as highgrade liver fibrosis versus localized fibrosis and low level of AT1R expression among UC-MSCs treated-group (p=0.001). Conclusion UC-MSCs administration could ameliorate liver fibrosis by reducing the AT1R expression and PDGF-β serum levels, and intervention through this signaling pathway could be alternative evidence for the causative of positive outcome.
Taufik Sungkar, Agung Putra, Dharma Lindarto, Rosita Juwita Sembiring

2041 related Products with: Anti-fibrotic effect of intravenous umbilical cord-derived mesenchymal stem cells (UC-MSCs) injection in experimental rats induced liver fibrosis.

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#33078199   2020/10/20 To Up

Lower mgpB diversity in macrolide-resistant Mycoplasma genitalium infecting men visiting two sexually transmitted infection clinics in Montpellier, France.

Men engaged in high-risk sexual behaviour, such as MSM, are likely to be infected by resistant Mycoplasma genitalium strains. Understanding the transmission dynamics is challenging. We aimed to investigate the molecular epidemiology of M. genitalium in men visiting sexually transmitted infection (STI) clinics.
Jennifer Guiraud, Manon Lounnas, Anne Boissière, Chloé Le Roy, Eric Elguero, Anne Laure Banuls, Cécile Bébéar, Sylvain Godreuil, Sabine Pereyre

1587 related Products with: Lower mgpB diversity in macrolide-resistant Mycoplasma genitalium infecting men visiting two sexually transmitted infection clinics in Montpellier, France.

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#33077628   2020/10/19 To Up

Antibody Opsonization Enhances Early Interactions between and Neutrophils in the Skin and Draining Lymph Node in a Mouse Model of Bubonic Plague.

Bubonic plague results when is deposited in the skin via the bite of an infected flea. Bacteria then traffic to the draining lymph node (dLN) where they replicate to large numbers. Without treatment, this infection can result in a highly fatal septicemia. Several plague vaccine candidates are currently at various stages of development, but no licensed vaccine is available in the United States. Though polyclonal and monoclonal antibodies (Ab) can provide complete protection against bubonic plague in animal models, the mechanisms responsible for this antibody-mediated immunity (AMI) to remain poorly understood. Here we examine the effects of Ab opsonization on interactions with phagocytes and Opsonization of with polyclonal antiserum modestly increased phagocytosis/killing by and oxidative burst of murine neutrophils Intravital microscopy (IVM) showed increased association of Ab opsonized with neutrophils in the dermis in a mouse model of bubonic plague. IVM of popliteal LNs after i.d. injection of bacteria in the footpad revealed increased -neutrophil interactions and increased neutrophil crawling and extravasation in response to Ab-opsonized bacteria. Thus, despite only having a modest effect in assays, opsonizing Ab had a dramatic effect on -neutrophil interactions in the dermis and dLN very early after infection. These data shed new light on the importance of neutrophils in AMI to and may provide a new correlate of protection for evaluation of plague vaccine candidates.
Jeffrey G Shannon, B Joseph Hinnebusch

2621 related Products with: Antibody Opsonization Enhances Early Interactions between and Neutrophils in the Skin and Draining Lymph Node in a Mouse Model of Bubonic Plague.

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#33072105   2020/09/23 To Up

The Next Frontier of Regulatory T Cells: Promising Immunotherapy for Autoimmune Diseases and Organ Transplantations.

Regulatory T cells (Tregs) are crucial in maintaining tolerance. Hence, Treg immunotherapy is an attractive therapeutic option in autoimmune diseases and organ transplantations. Currently, autoimmune diseases do not have a curative treatment and transplant recipients require life-long immunosuppression to prevent graft rejection. There has been significant progress in understanding polyclonal and antigen-specific Treg biology over the last decade. Clinical trials with good manufacturing practice (GMP) Treg cells have demonstrated safety and early efficacy of Treg therapy. GMP Treg cells can also be tracked following infusion. In order to improve efficacy of Tregs immunotherapy, it is necessary that Tregs migrate, survive and function at the specific target tissue. Application of antigen specific Tregs and maintaining cells' suppressive function and survival with low dose interleukin-2 (IL-2) will enhance the efficacy and longevity of infused GMP-grade Tregs. Notably, stability of Tregs in the local tissue can be manipulated by understanding the microenvironment. With the recent advances in GMP-grade Tregs isolation and antigen-specific chimeric antigen receptor (CAR)-Tregs development will allow functionally superior cells to migrate to the target organ. Thus, Tregs immunotherapy may be a promising option for patients with autoimmune diseases and organ transplantations in near future.
Lauren V Terry, Ye Htun Oo

2154 related Products with: The Next Frontier of Regulatory T Cells: Promising Immunotherapy for Autoimmune Diseases and Organ Transplantations.



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#33070097   2020/09/15 To Up

ElectrochemSENSE: A platform towards field deployable direct on-produce glyphosate detection.

Glyphosate is an organophosphorus herbicide that is applied to the leaves of plants and crops to kill broadleaf plants and grasses. In this paper, for the first time, a field deployable, user-friendly, portable and rapid electrochemical pesticide sensing system is presented that can screen for glyphosate in produce run-off/extract. ElectrochemSENSE comprises the following parts: A polymer based disposable substrate with metallized electrodes that are surface treated with polyclonal antibodies of glyphosate and a custom electronic reader capable of reporting pesticide contamination. Utilizing the principles of capacitive current changes due to selective binding of glyphosate to its capture probe, reporting was achieved rapidly (in under 5 min). ElectrochemSENSE was tested to screen for glyphosate concentrations on produce samples above or below the globally accepted metric criterion, otherwise known as the Maximum Residue Level (MRL). Experiments were conducted on 4 produce types-apples (MRL: 0.2 ppm), strawberries (MRL: 0.2 ppm), bell peppers (MRL: 0.1 ppm) and carrots (MRL: 5 ppm). To further add functionality and increase prediction accuracy- a machine learning binary classifier was integrated with the device as a proof-of-concept so that sensor's response can be trained and characterized to perform with high accuracy, thereby serving as an analytics medium which minimizes error rate. Utilizing this system-the sensor's limit of detection has been determined to be 0.01 ppm (10 ng/mL) considering the permissible Field Operating Range (FOR) for glyphosate residue in various tested produce.
Vikram Narayanan Dhamu, Shalini Prasad

1018 related Products with: ElectrochemSENSE: A platform towards field deployable direct on-produce glyphosate detection.

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#33068703   2020/10/14 To Up

Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2.

Towards the end of 2019, a novel coronavirus (CoV) named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), genetically similar to severe acute respiratory syndrome coronavirus (SARS-CoV), emerged in Wuhan, Hubei province of China, and has been responsible of coronavirus disease 2019 (COVID-19) in humans. Since its first report, SARS-CoV-2 has resulted in a global pandemic, with over 10 million human infections and over 560,000 deaths reported worldwide at the end of June 2020. Currently, there are no United States (US) Food and Drug Administration (FDA)-approved vaccines and/or antivirals licensed against SARS-CoV-2, and the high economical and health impact of SARS-CoV-2 has placed global pressure on the scientific community to identify effective prophylactic and therapeutic treatments for the treatment of SARS-CoV-2 infection and associated COVID-19 disease. While some compounds have been already reported to reduce SARS-CoV-2 infection and a handful of monoclonal antibodies (mAbs) have been described that neutralize SARS-CoV-2, there is an urgent need for the development and standardization of assays which can be used in high through-put screening (HTS) settings to identify new antivirals and/or neutralizing mAbs against SARS-CoV-2. Here, we described a rapid, accurate and highly reproducible plaque reduction microneutralization (PRMNT) assay that can be quickly adapted for the identification and characterization of both neutralizing mAbs and antivirals against SARS-CoV-2. Importantly, our MNA is compatible with HTS settings to interrogate large and/or complex libraries of mAbs and/or antivirals to identify those with neutralizing and/or antiviral activity, respectively, against SARS-CoV-2.
Jun-Gyu Park, Fatai S Oladunni, Kevin Chiem, Chengjin Ye, Michael Pipenbrink, Thomas Moran, Mark R Walter, James Kobie, Luis Martinez-Sobrido

1068 related Products with: Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2.

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#33068629   2020/10/14 To Up

Abnormal characteristic "round bottom flask" shape volume-based scattergram as a trigger to suspect persistent polyclonal B-cell lymphocytosis.

The diagnosis of persistent polyclonal B-cell lymphocytosis (PPBL) is often challenging because of the lack of features and the overlap with the peripheral expression of splenic marginal zone lymphomas (SMZL). To obtain new clues for PPBL detection and diagnosis, all data provided by the DxH 800 analyzer (including scatter and cell population data (CPD)) was exploited and combined using a machine learning (ML) approach.
Laura Bigorra, Iciar Larriba, Ricardo Gutiérrez-Gallego

2217 related Products with: Abnormal characteristic "round bottom flask" shape volume-based scattergram as a trigger to suspect persistent polyclonal B-cell lymphocytosis.

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#33068192   2020/10/17 To Up

Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus.

Aleutian mink disease (AMD), caused by Aleutian mink disease virus (AMDV), is a very important infectious disease of mink. Currently, elimination of antibody- or antigen-positive animals is the most successful strategy for eradicating AMD, but the claw-cutting method of blood sampling is difficult to perform and painful for the animal. In this study, we aimed to establish an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) method for the efficient detection of AMDV antigens using fecal samples. A purified mouse monoclonal antibody (mAb) was used as the capture antibody, and a rabbit polyclonal antibody (pAb) was used as the detection antibody. The assay was optimized by adjusting a series of parameters. Using a cutoff value of 0.205, the limit of detection of the AC-ELISA for strain AMDV-G antigen was 2 μg/mL, and there was no cross-reaction with other mink viruses. The intra- and inter-assay standard deviations were below 0.046, and the correlation of variance (CV) values were 1.24-7.12% when testing fecal samples. Compared with conventional PCR results, the specificity and sensitivity were 91.5% and 90.6%, respectively, and the concordance rate between the two methods was 91.1%.
Taofeng Lu, Yuanzhi Wang, Yanjun Wu, Lili Zhao, Shuguang Wu, Hongyan Chen

1155 related Products with: Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus.

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#33061632   2020/09/30 To Up

Simultaneous Presentation of Leukemic Non-Nodal Mantle Cell Lymphoma and Gamma-Delta T-Large Granular Lymphocytic Leukemia in a Patient with Rheumatoid Arthritis.

The peculiar features of T-cell large granular lymphocytic leukemia (T-LGLL) are its association with autoimmune disorders (particularly with rheumatoid arthritis (RA)) and a broad spectrum of B-cell lymphoproliferative disorders. However, association of T-LGLL with mantle cell lymphoma (MCL) is extremely rare. Here, we describe a case of an 80-year-old man admitted with suspected Felty's syndrome. The blood count showed white blood cells at 2.2×10/L, with 3% neutrophils, 88% lymphocytes, and at 0.66×10/L LGLs. The spleen had been removed 43 months prior to the admission due to suspected B-cell splenic lymphoma. Re-examination of the spleen revealed cyclin D1+ and SOX11- lymphocytes in the inner part of the unexpanded mantle zones of the white pulp follicles, thus displaying a so-called in situ histologic pattern of MCL, and in small clusters in the red pulp. The splenic cords were moderately expanded by lymphocytes expressing CD3, TIA1, and granzyme B but not CD4 and CD8. Monoclonal rearrangements of the gene and the genes, polyclonal rearrangements of the gene, mutation of the   gene (c.1940A>T; p.N647I), and t(11;14)(q13;q32) translocation were identified in the spleen sample. Flow cytometry of bone marrow revealed a population of TCR γδ+, CD3+, CD4-, CD5-, CD7+, CD8-, CD16-, CD56-, and CD57- lymphocytes. Fragment analysis demonstrated identical gene clonal rearrangement patterns in the spleen and bone marrow samples. In this study, we describe the first case of simultaneous presentation of γδ T-LGLL and leukemic non-nodal MCL (L-NN-MCL) in a patient with RA and present morphological findings of L-NN-MCL in the spleen.
Vadim R Gorodetskiy, Natalya A Probatova, Natalia A Kupryshina, Svetlana G Palshina, Tatiana N Obukhova, Yulia V Sidorova, Natalya V Ryzhikova, Andrey B Sudarikov

2866 related Products with: Simultaneous Presentation of Leukemic Non-Nodal Mantle Cell Lymphoma and Gamma-Delta T-Large Granular Lymphocytic Leukemia in a Patient with Rheumatoid Arthritis.

24 tests100ug Lyophilized100 μg0.5 ml

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