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#35045371   2022/01/04 To Up

Metal-organic frameworks with peroxidase-like activity for efficient removal of aflatoxin B.

Aflatoxin B (AFB), a naturally produced toxin existing in major food crops, is highly toxic and carcinogenic to human and animals. In this study, a reusable material, [email protected] with great adsorption performance and peroxidase-like activity was synthesized for the removal of AFB. [email protected] exhibited great adsorption performance owing to hierarchical porous structure. [email protected] also could catalyze the AFB in the presence of HO due to the Fe-tetrakis (4-carboxyphenyl) porphyrin and Pd as effective peroxidase active site, which improved the removal efficiency of AFB. [email protected] was applied for the removal of AFB with a removal rate of 96.52% in 2 h. Owing to the advantages of high removal efficiency and reusability, [email protected] had great application potential in AFB removal.
Jinhui Wei, Xiangchuan Wu, Chengyuan Wu, Fan Hou, Lina Wu, He Huang

2978 related Products with: Metal-organic frameworks with peroxidase-like activity for efficient removal of aflatoxin B.

100ul100 assays100ul50 mL15 ml100μl100ug Lyophilized96 assays 1 G200 Tests

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#35045370   2022/01/11 To Up

Preparation of highly stable oleogel-based nanoemulsions for encapsulation and controlled release of curcumin.

Oleogels have been proposed as suitable systems for the encapsulation and delivery of lipophilic bioactive compounds. This work aimed to produce stable nanoemulsions of gelled-oil particles using monoglyceride (MG) oleogels loaded with curcumin. High-speed homogenization followed by ultrasonication was used for obtaining colloidal dispersions. The effects of ultrasonication processing parameters and formulation were evaluated to optimize particle size, polydispersity index (PDI), and stability during storage. All sonication parameters had a significant effect on particle size and PDI. A Pluronic F-68 + Tween 80 surfactant mixture with the lowest oleogel/aqueous phase ratio (5/95) produced nanoemulsions which were at least 10-month stable. The nanoemulsions showed a higher encapsulation efficiency than the sample without the gelator (73.85-91.05% vs. 56.99%). Furthermore, it was corroborated that structuring oil particles with MG crystals produces a matrix that entraps curcumin molecules and slows down their release. These findings provide useful information for the development of new nutraceutical products.
Camila A Palla, Aixa Aguilera-Garrido, María Elena Carrín, Francisco Galisteo-González, María José Gálvez-Ruiz

2607 related Products with: Preparation of highly stable oleogel-based nanoemulsions for encapsulation and controlled release of curcumin.

5 G1000 assays100 assays1000 assays100preparations100tests100 assays150 ug10000 assays

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#35045340   2022/01/16 To Up

Impact of sepsis education for healthcare professionals and students on learner and patient outcomes: A systematic review.

Sepsis is an important global healthcare problem that is a key challenge faced by healthcare professionals face worldwide. One key effort aimed at reducing the global burden of sepsis is educating healthcare professionals about early identification and management of sepsis.
Chloe Leyi Choy, Sok Ying Liaw, Ee Ling Goh, Kay Choong See, Wei Ling Chua

2209 related Products with: Impact of sepsis education for healthcare professionals and students on learner and patient outcomes: A systematic review.

1000 tests50 ug 100 mg100ul10 mg100ul500 MG25 mg100ug96T100ug50 mg

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#35045328   2022/01/16 To Up

Co-administration of rotavirus nanospheres VP6 and NSP4 proteins enhanced the anti-NSP4 humoral responses in immunized mice.

Inconveniences associated with the efficacy and safety of the World Health Organization (WHO) approved/prequalified live attenuated rotavirus (RV) vaccines, sounded for finding alternative non-replicating modals and proper RV antigens (Ags). Herein, we report the development of a RV candidate vaccine based on the combination of RV VP6 nanospheres (S) and NSP4 proteins (VP6S + NSP4). Self-assembled VP6S protein was produced in insect cells. Analyses by western blotting and transmission electron microscopy (TEM) indicated expression of VP6 trimer structures with sizes of ≥140 kDa and presence of VP6S. Four group of mice were immunized (2-dose formulation) intra-peritoneally (IP) by either¨VP6S + NSP4¨ or each protein alone (VP6S or NSP4) emulsified in aluminium hydroxide or control. Results indicated that VP6S + NSP4 formulation induced significant anti-VP6 IgG (P < 0.001) and IgA (P < 0.05) as well as anti-NSP4 IgG (P < 0.001) and enhancement of protective immunity. Analyses of anti-VP6S and anti-NSP4 IgG subclass (IgG1 and IgG2a) showed IgG1/IgG2a ≥6 and IgG1/IgG2a ≥3 ratios, respectively indicating Th2 polarization of immune responses. The combination of VP6S + NSP4 proteins emulsified in aluminum hydroxide adjuvant might present a dual universal, efficient and cost-effective candidate vaccine against RV infection.
Atefeh Afchangi, Somayeh Jalilvand, Arash Arashkia, Tayebeh Latifi, Mohammad Farahmand, Maryam Mashhadi Abolghasem Shirazi, Seyed Dawood Mousavi Nasab, Sayed Mahdi Marashi, Farzin Roohvand, Zabihollah Shoja

2304 related Products with: Co-administration of rotavirus nanospheres VP6 and NSP4 proteins enhanced the anti-NSP4 humoral responses in immunized mice.

25mg100ug100 μg1mg1 mg100 μg100ug Lyophilized

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#35045265   2022/01/19 To Up

Antiviral Activity and Resistance Profile of the Novel HIV-1 Non-Catalytic Site Integrase Inhibitor, JTP-0157602.

HIV-1 integrase (IN) is an essential enzyme for viral replication. Non-catalytic site integrase inhibitors (NCINIs) are allosteric HIV-1 IN inhibitors and a potential new class of antiretrovirals. In this report, we identified a novel NCINI, JTP-0157602, with an original scaffold. JTP-0157602 exhibited potent antiviral activity against HIV-1 and showed a serum-shifted EC of 138 nM, which is comparable to the FDA-approved IN strand transfer inhibitors (INSTIs). This compound was fully potent against a wide range of recombinant viruses with IN polymorphisms, including amino acids 124/125, a hot spot of IN polymorphisms. In addition, JTP-0157602 retained potent antiviral activity against a broad panel of recombinant viruses with INSTI-related resistant mutations, including multiple substitutions that emerged in clinical studies of INSTIs. Resistance selection experiments of JTP-0157602 led to the emergence of A128T and T174I mutations, which are located at the lens epithelium-derived growth factor/p75 binding pocket of IN. JTP-0157602 inhibited HIV-1 replication mainly during the late-phase of the replication cycle, and HIV-1 virions produced by reactivation from HIV-1 latently-infected Jurkat cells in the presence of JTP-0157602 were non-infectious. These results suggest that JTP-0157602 and analog compounds can be used to treat HIV-1 infectious diseases. Non-catalytic site integrase inhibitors (NCINIs) are allosteric HIV-1 integrase (IN) inhibitors that bind to the lens epithelium-derived growth factor (LEDGF)/p75 binding pocket of IN. NCINIs are expected to be a new class of anti-HIV-1 agents. In this study, we present a novel NCINI, JTP-0157602, which has potent activity against a broad range of HIV-1 strains with IN polymorphisms. Furthermore, JTP-0157602 shows strong antiviral activity against IN strand transfer inhibitor-resistant mutations, suggesting JTP-0157602 and its analogs are potential agents to treat HIV-1 infections. Structural modeling indicated that JTP-0157602 binds to the LEDGF/p75 binding pocket of IN, and the results of resistance induction revealed the JTP-0157602-resistance mechanism of HIV-1. These data shed light on developing novel NCINIs, which exhibit potent activity against HIV-1 with broad IN polymorphisms and multi-drug resistant HIV-1 variants.
Yoshitsugu Ohata, Mitsunori Tomonaga, Yasuo Watanabe, Keiko Tomura, Koji Kimura, Tatsuo Akaki, Kaoru Adachi, Eiichi N Kodama, Yuji Matsuzaki, Hironori Hayashi

1105 related Products with: Antiviral Activity and Resistance Profile of the Novel HIV-1 Non-Catalytic Site Integrase Inhibitor, JTP-0157602.

100μg100ug1000 10mg1x96 well plate100μg100ul100ul100μg5mg100ug Lyophilized10mg

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#35045209   2022/01/19 To Up

Surface dynamics, fractal features, and micromorphology analysis of kefir biofilms.

We introduce a study of image analysis of kefir biofilms associated with Acai extract prepared by fermentation of fresh kefir grains natural. Atomic force microscopy data were studied, aiming to understand how the concentration of acai berry (Euterpe oleracea Mart.) influences the surface morphology as well as the texture complexity, evaluated by the fractal dimension. The results showed that the superficial morphology was affected by the increase of Acai concentration in the biofilms, as well as the fractal dimension. It has also been observed that the surface of the biofilm presented saturation when concentration changes from 40 to 60 ml. On the other hand, it was observed that the intermediate sample produced with 20 ml of acai berry seems to be the best point for biofilms production that can serve as a skin dressing since other studies related to mechanical properties and in vitro and in vivo tests can confirm this applicability. Thus, the characterization of the surface morphology of kefir biofilms by the evaluation of surface statistical parameters and fractal geometry may provide promising results regarding the applicability of these films.
Ştefan Ţălu, Erveton P Pinto, Robert S Matos, Amir Zelati, Shahram Solaymani, Azizollah Shafiekhani, Atefeh Ghaderi

1151 related Products with: Surface dynamics, fractal features, and micromorphology analysis of kefir biofilms.

1 module1 module1 module1000 tests100ug3 modules0.1 mg 15 ml 100ug1 module1 mg

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#35045151   2022/01/19 To Up

Role of genistein on the yield, structure and immunomodulatory activity of exopolysaccharides.

Manipulating the structures, physicochemical properties, and monosaccharide compositions of exopolysaccharides (EPS) isolated from microorganisms has been reported to enhance their biological activities. Hence, the aim of this work was to examine the effects of genistein addition during fermentation on the amount, physicochemical properties, and immunomodulatory activity of EPS secreted by . Results showed that genistein addition significantly increased biomass and EPS yield to 2.42 g L and 6.08 g L, respectively, and affected the physicochemical properties and structures of EPS. Furthermore, EPS produced by genistein-treated (G-EMP) improved the immunomodulatory activity of RAW264.7 macrophages by increasing the secretion of nitric oxide and cytokines. Moreover, phospho-Jun N-terminal kinase (p-JNK), phospho-extracellular regulated protein kinase (p-ERK), phospho-p38 (p-p38) mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) phospho-p65 (p65) proteins were remarkably upregulated by G-EMP stimulation, blocking Toll-like receptor 4 (TLR4) that dramatically reduced the pinocytic and phagocytic capacities. Overall, these findings provide potential rationales for the application of genistein in improving the EPS yield of .
Liuming Xie, Zhibing Huang, Hui Meng, Zhibing Fan, Xiaoyi Shi, Jianhua Xie

2802 related Products with: Role of genistein on the yield, structure and immunomodulatory activity of exopolysaccharides.

5 G100 assays100.00 ul100ug1,000 tests40 assays100ul50 ug 48 assays 96 Tests

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#35045114   2022/01/19 To Up

The influence of food processing methods on serum parameters, apparent total-tract macronutrient digestibility, fecal microbiota and SCFA content in adult beagles.

Food processing methods may influence the health of dogs. However, previous studies have mostly been based on a comparison of several commercial dog foods with different ingredients. In this study, eighteen adult beagles of the same age and health status (assessed by routine blood tests) were used in the experiments. This study analyzed the effects of the following different processing methods: raw, pasteurized, and high temperature sterilization (HTS) made with the same ingredients and nutrients (based on dry matter) on serum parameters, apparent total-tract macronutrient digestibility, fecal microbiota and short-chain fatty acid (SCFA) content in beagle dogs. The data showed, after a test lasting 56-days, the apparent digestibility (ATTD) of protein and fat in HTS food was 91.9%, which was significantly higher (P< 0.05) than that in dry food (89.2%, P < 0.05). The serum content of triglyceride increased in beagles fed HTS food (P < 0.05), and the number of neutrophils in beagles fed raw food and pasteurized food increased significantly (P < 0.05), and the platelet count in beagles fed raw food showed an increasing trend compared with the beagles fed HTS food. Different processing methods had an impact on the intestinal microbiota and SCFA of beagles; at least 14 genera were significantly affected by the food produced using different processing methods. In particular, the abundance of Allprevotella, Escherichia-Shigella and Turicibacter, and the total acid content were lower in beagles fed the raw diet, whereas Streptococcus, Collinsella, Bacteroides and Ruminococcus gnavus were more abundant following the HTS diet, and Lactococcus showed the highest abundance in beagles fed the pasteurized diet. This study showed that dog food produced by different processing methods affected the health of adult beagles.
Xuan Cai, Rongrong Liao, Guo Chen, Yonghong Lu, Yiqun Zhao, Yi Chen

1546 related Products with: The influence of food processing methods on serum parameters, apparent total-tract macronutrient digestibility, fecal microbiota and SCFA content in adult beagles.

50 ml96 tests100ug500 ml10ml10 ml

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#35045085   2022/01/19 To Up

Antioxidant activities of some monofloral honey types produced across Minas Gerais (Brazil).

This study was carried out with the objective of determining the antioxidant properties and quantification of total phenolics and flavonoids in relation to quercetin and rutin in some of the monofloral honeys produced in Minas Gerais (Brazil). In this study, 15 monofloral honey samples were obtained from different geographic regions of Minas Gerias, Brazil. The honeys were obtained from Cooperative of Beekeepers and Family Farmers of Northern Minas. To determine the antioxidant properties of honey samples, the test methods of total phenolic content, flavonoids (rutin and quercetin) and DPPH were used. As a result of the analysis of phenolic and flavonoid contents, the samples with the best results were A1-Aroeira and A4-Assa peixe. In antioxidant activity, the honey with the best EC50 results was A6-Aroeira. Differences between the antioxidant activities of the honey samples were found significantly (p< 0.01).
Deosvaldo S Pena Júnior, Clarice A Almeida, Maria Clara F Santos, Pedro Henrique V Fonseca, Elytania V Menezes, Afranio F de Melo Junior, Murilo M Brandão, Dario A de Oliveira, Luciano F de Souza, Junio C Silva, Vanessa de A Royo

1346 related Products with: Antioxidant activities of some monofloral honey types produced across Minas Gerais (Brazil).

100ul100ul100ug100ug100ug1mg100ul100ul100ul100ug100ul

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#35045073   2022/01/19 To Up

Human unintentional and intentional interpersonal coordination in interaction with a humanoid robot.

In order to establish natural social synchrony between two humans, two requirements need to be fulfilled. First, the coupling must be bi-directional. The two humans react to each other's actions. Second, natural social bodily synchronization has to be intentional or unintentional. Assuming that these essential aspects of human-human interactions are present, the present paper investigates whether similar bodily synchrony emerges between an interacting human and an artificial agent such as a robot. More precisely, we investigate whether the same human unintentional rhythmic entrainment and synchronization is present in Human Robot Interaction (HRI). We also evaluate which model (e.g., an adaptive vs non adaptive robot) better reproduces such unintentional entrainment. And finally, we compare interagent coordination stability of the HRI under 1) unidirectional (robot with fixed frequency) versus bidirectional (robot with adaptive frequency) rhythmic entrainment and 2) human intentional versus unintentional coupling. Fifteen young adults made vertical arm movements in front of the NAO robot under five different conditions of intentional/unintentional and unidirectional/bidirectional interactions. Consistent with prior research investigating human-human interpersonal coordination, when humans interact with our robot, (i) unintentional entrainment was present, (ii) bi-directional coupling produced more stable in-phase un-intentional and intentional coordination, (iii) and intentional coordination was more stable than unintentional coordination. To conclude, this study provides a foundation for modeling future social robots involving unintentional and bidirectional synchronization-aspects which seem to enhance humans' willingness to interact with robots.
Ghiles Mostafaoui, R C Schmidt, Syed Khursheed Hasnain, Robin Salesse, Ludovic Marin

1597 related Products with: Human unintentional and intentional interpersonal coordination in interaction with a humanoid robot.

1 Set1 Set1 Set100 μg1 Set100 μg1 Set1 Set1 Set 100 UG100 μg100 μg

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