Product name :Tfi DNA Ligase Includes with dNTPs

Catalog Number :E-3111

Quantity :2000 Units

Price :115 Eur

Pay now with :

Supplier :Bioneer

 

Application

- Ligase Chain Reaction (LCR)
- Oligonucleotide Ligation Assay (OLA)
- Mutagenesis by Incorporation of a phosphorylated oligo during PCR Amplification
- Simultaneous Mutagenesis of Multiple Sites  


Supplied with Enzyme

- 10 x Reaction Buffer (1 ml):  300 mM Tris-HCl (pH8.3), 250 mM KCl, 50 mM MgCl2, 5 mM NAD
- 1 x Dilution Buffer (1 ml):  10 mM Tris-HCl (pH7.6), 0.1 mM EDTA, 50 mM KCl, 1 mM DTT, 200 ug/mL acetylated BSA,
   50% Glycerol


Storage Condition

20 mM Tris-HCl (pH7.6), 2 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.5% Tween -20, 0.5% IGEPAL CA-630, 50% Glycerol, store at -20°C.


Concentration

20 units/μl


Unit definition

One unit of Tfi DNA Ligase is defined as the amount of enzyme required to give 50% ligation of the 12 base pair cohesive ends of 1 ug of PspEI digested lambda DNA in 10min at 45°C.


Activity Assay Conditions

The activity assay is carried out in a 20 μl reaction containing 1 ug of PspEI digested lambda DNA and 1 x Tfi DNA ligase reaction buffer. After incubation at 45°C for 10 min., the reaction is terminated by addition of stop solution (40%(w/v) sucrose, 50 mM EDTA and 0.25% bromophenol blue). Then heat at 70°C for 10 min and immediately load on a 0.8% agarose gel.


Stability

The half-life of the enzyme in 1 x reaction buffer is more than 1 hour at 95°C and 55 hours at 65°C.


Note

Tfi DNA Ligase should not be used as a substitute for other DNA ligase, i.e., T4 DNA Ligase.


References

1. Barany, F. (1991) Proc. Natl. Acad. Sci. USA, 88, 189 - 193. 
2. Landegren, U. et al.(1988) Science 241, 1077 - 1080
3. Michael, Scott F. (1994) Biotechniques 16:3, 410 - 412.
4.Gerard J. A. et al. (1993) Biotechniques 15:1, 172 - 178.


Experimental Data

Fig 1. Ligation test at various temperatures (45°C~ 65°C)
figure1
Incubate the reaction containing ligase 1 unit and 1 ug DNA[lambda PspEI] at each temperature for 10 min.
Lane 1: λ DNA/PspE I (control)         Lane 2: Incubate at 45°C, 10 min
Lane 3: Incubate at 50°C, 10 min      Lane 4: Incubate at 55°C, 10 min
Lane 5: Incubate at 60°C, 10 min      Lane 6: Incubate at 65°C, 10 min


Fig 2. Heat Stability test at 95°C
figure2
Incubate the enzyme at 95°C each time. And then add 1 unit ligase to a 20 ul reaction containing 1ug DNA[lambda PspEI] and incubate the mixture at 45°C for 10 min. 
Lane 1: λ DNA/PspE I (control)         Lane 2: Incubate at 95°C, 10 min 
Lane 3: Incubate at 95°C, 20 min      Lane 4: Incubate at 95°C, 30 min 
Lane 5: Incubate at 95°C, 40 min      Lane 6: Incubate at 95°C, 50 min 
Lane 7: Incubate at 95°C, 60 min      Lane 8: Incubate at 95°C, 70 min 
Lane 9: Incubate at 95°C, 80 min      Lane 9: Incubate at 95°C, 90 min


Fig 3. Heat Stability test at 65°C
figure3
Incubate the enzyme for each time at 65°C and then add 1 unit ligase to a 20 ul reaction containing 1 ug DNA[lambda PspEI] and incubate the mixture at 4°C for 10 min.
Lane 1: λ DNA/PspE I (control)           Lane 2: Incubate at 65°C, 6 hrs
Lane 3: Incubate at 65°C, 12 hrs        Lane 4: Incubate at 65°C, 18 hrs 
Lane 5: Incubate at 65°C, 24 hrs        Lane 6: Incubate at 65°C, 30 hrs 
Lane 7: Incubate at 65°C, 36 hrs        Lane 8: Incubate at 65°C, 42 hrs 
Lane 9: Incubate at 65°C, 48 hrs        Lane 10: Incubate at 65°C, 54 hrs
Lane 11: Incubate at 65°C, 60 hrs      Lane 12: Incubate at 65°C, 66 hrs 
Lane 13: Incubate at 65°C, 72 hrs      Lane 14:Incubate at 65°C, 78 hrs

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AA0030 Insulin Glucose Phospho-Specific Array includes 85 highly specific and well-characterized phosphorylation antibodies in the insulin glucose pathway. Abnova 2 Pieces/Box 731 AA0030 Insulin Glucose Phospho-Specific Array includes 85 highly specific and well-characterized phosphorylation antibodies in the insulin glucose pathway. Abnova 2 Pieces/Box 731€
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AA0094 MMP (Human) Antibody Array includes 10 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0094 MMP (Human) Antibody Array includes 10 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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228-10329-3 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 100 1312 228-10329-3 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 100 1312€
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02-050-5 T4 DNA Ligase, standard kit T4 DNA Ligase, standard kit B-Bridge 5 X 20,000 U 201.6 02-050-5 T4 DNA Ligase, standard kit T4 DNA Ligase, standard kit B-Bridge 5 X 20,000 U 201.6€
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SP1800 Syringe pump can be controlled manually or with a computer. Good for infusion, withdrawal or up to 41 programable pumping phases. Stall detection. Allows pumping of 8 syringes at a time. Next Advence 1610 SP1800 Syringe pump can be controlled manually or with a computer. Good for infusion, withdrawal or up to 41 programable pumping phases. Stall detection. Allows pumping of 8 syringes at a time. Next Advence 1610€ Pub
AA0062 Inflammation (Mouse) Antibody Array (G) includes 40 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0062 Inflammation (Mouse) Antibody Array (G) includes 40 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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22027 DNAI2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329 22027 DNAI2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329€
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bs-7653R-A350 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 350 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323 bs-7653R-A350 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 350 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323€ Pub
AA0039 PDGF Phospho-Specific Array includes 195 highly specific and well-characterized phosphorylation antibodies in the PDGF pathway. Abnova 2 Pieces/Box 822 AA0039 PDGF Phospho-Specific Array includes 195 highly specific and well-characterized phosphorylation antibodies in the PDGF pathway. Abnova 2 Pieces/Box 822€
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E-2015 Pfu DNA Polymerase (Not available for sale in USA) Without dNTPs, 10 x reaction buffer Bioneer 250 Units 217 E-2015 Pfu DNA Polymerase (Not available for sale in USA) Without dNTPs, 10 x reaction buffer Bioneer 250 Units 217€ Pub
228-10332-3 Recombinant Human HSP70 DnaK Proteins Ray Biotech 1mg 1782 228-10332-3 Recombinant Human HSP70 DnaK Proteins Ray Biotech 1mg 1782€
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9101-250 T4 DNA Ligase (5 u μl) Biovision 250 u 149 9101-250 T4 DNA Ligase (5 u μl) Biovision 250 u 149€
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AA0071 Chemokine (Human) Antibody Array includes 38 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0071 Chemokine (Human) Antibody Array includes 38 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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22071 DNAJB2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329 22071 DNAJB2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329€
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bs-1276R-Cy3 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Cy3 Conjugated Bioss 100ug 369 bs-1276R-Cy3 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Cy3 Conjugated Bioss 100ug 369€ Pub
bs-7653R-FITC Rabbit Anti-DNase gamma Polyclonal Antibody, FITC conjugated,Isotype: IgG Sepax 100ug Lyophilized 304 bs-7653R-FITC Rabbit Anti-DNase gamma Polyclonal Antibody, FITC conjugated,Isotype: IgG Sepax 100ug Lyophilized 304€ Pub
AA0048 Cytokine (Human) Antibody Array (G) includes 80 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888 AA0048 Cytokine (Human) Antibody Array (G) includes 80 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888€
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E-3100-2 includes 10 x reaction buffer with MgCl2 Bioneer 500 Units 135 E-3100-2 includes 10 x reaction buffer with MgCl2 Bioneer 500 Units 135€ Pub
228-10335-3 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 1mg 1031 228-10335-3 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 1mg 1031€
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AA0015 Apoptosis Phospho-Specific Array includes 247 highly specific and well-characterized phosphorylation antibodies i related to the proteins regulating apoptosis. Abnova 2 Pieces/Box 822 AA0015 Apoptosis Phospho-Specific Array includes 247 highly specific and well-characterized phosphorylation antibodies i related to the proteins regulating apoptosis. Abnova 2 Pieces/Box 822€
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AA0080 Cytokine (Human) Antibody Array includes 49 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888 AA0080 Cytokine (Human) Antibody Array includes 49 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888€
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AA0089 Growth Factor (Human) Antibody Array includes 41 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0089 Growth Factor (Human) Antibody Array includes 41 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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bs-1276R-PE-Cy5.5 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG Sepax 100ug Lyophilized 300 bs-1276R-PE-Cy5.5 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG Sepax 100ug Lyophilized 300€
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G028 DNase I ABM Goods 1ml / 2000U 71.4 G028 DNase I ABM Goods 1ml / 2000U 71.4€ Pub
AA0057 Cytokine (Mouse) Antibody Array (G) includes 48 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0057 Cytokine (Mouse) Antibody Array (G) includes 48 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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E-3122 5 tubes of 10,000 Units Includes 5 X reaction buffer, 100 mM DTT Bioneer 50000 Units 323 E-3122 5 tubes of 10,000 Units Includes 5 X reaction buffer, 100 mM DTT Bioneer 50000 Units 323€ Pub
22663 DNase I antibody Source Rabbit Polyconal Ab Species Human Application WB IF SignalWay Antibody S.A.B 100ul 329 22663 DNase I antibody Source Rabbit Polyconal Ab Species Human Application WB IF SignalWay Antibody S.A.B 100ul 329€
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AA0024 ErbB Her Signaling Phospho-Specific Array includes 239 highly specific and well-characterized phosphorylation antibodies in the ErbB Her pathway. Abnova 2 Pieces/Box 822 AA0024 ErbB Her Signaling Phospho-Specific Array includes 239 highly specific and well-characterized phosphorylation antibodies in the ErbB Her pathway. Abnova 2 Pieces/Box 822€
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BAH67-0050 Protease, DNASE free heat shock BSA powder, 50gm Equitech 50gm 310.8 BAH67-0050 Protease, DNASE free heat shock BSA powder, 50gm Equitech 50gm 310.8€
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AA0034 mTOR Signalign Phospho-Specific Array includes 138 highly specific and well-characterized phosphorylation antibodies in the mTOR ignaling pathway. Abnova 2 Pieces/Box 776 AA0034 mTOR Signalign Phospho-Specific Array includes 138 highly specific and well-characterized phosphorylation antibodies in the mTOR ignaling pathway. Abnova 2 Pieces/Box 776€
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BB50AU-CE Homogenizer for 8 samples in 50mL tubes. Large enclosure to help reduce noise and active cooling to maintain 4C interior temperature. Includes starter kit of beads, scoops and tubes. Upgraded elect Next Advence 4985 BB50AU-CE Homogenizer for 8 samples in 50mL tubes. Large enclosure to help reduce noise and active cooling to maintain 4C interior temperature. Includes starter kit of beads, scoops and tubes. Upgraded elect Next Advence 4985€
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228-10331-1 Recombinant E. coli HSP70 DnaK ATPase BD Proteins Ray Biotech 10 147 228-10331-1 Recombinant E. coli HSP70 DnaK ATPase BD Proteins Ray Biotech 10 147€
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23073 TFIID (SI-1) antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329 23073 TFIID (SI-1) antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329€
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Y050476 Anti ACSL3(long chain fatty acid Coenzyme A ligase 3) ABM Goods 100ug 436.8 Y050476 Anti ACSL3(long chain fatty acid Coenzyme A ligase 3) ABM Goods 100ug 436.8€
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AA0066 Angiogenesis (Human) Antibody Array includes 23 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0066 Angiogenesis (Human) Antibody Array includes 23 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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228-10328-1 Recombinant Human HSP40 DnaJ Proteins Ray Biotech 5 147 228-10328-1 Recombinant Human HSP40 DnaJ Proteins Ray Biotech 5 147€
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bs-1276R-A350 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Alexa Fluor 350 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323 bs-1276R-A350 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Alexa Fluor 350 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323€ Pub
bs-7653R-Biotin Rabbit Anti-DNase gamma Polyclonal Antibody, Biotin conjugated, Isotype: IgG Sepax 100ug Lyophilized 268 bs-7653R-Biotin Rabbit Anti-DNase gamma Polyclonal Antibody, Biotin conjugated, Isotype: IgG Sepax 100ug Lyophilized 268€ Pub
AA0043 Angiogenesis (Human) Antibody Array (G) includes 20 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0043 Angiogenesis (Human) Antibody Array (G) includes 20 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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E-3041 T7 RNA Polymerase Includes 10 X reaction buffer, 100 mM DTT Bioneer 2000 Units 145 E-3041 T7 RNA Polymerase Includes 10 X reaction buffer, 100 mM DTT Bioneer 2000 Units 145€
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228-10334-1 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 20 147 228-10334-1 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 20 147€
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AA0005 Cytokine antibody array includes 77 highly specific and well-characterized antibodies in the cytokine pathway. Abnova 2 Pieces/Box 731 AA0005 Cytokine antibody array includes 77 highly specific and well-characterized antibodies in the cytokine pathway. Abnova 2 Pieces/Box 731€
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AA0075 Cytokine (Human) Antibody Array includes 80 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888 AA0075 Cytokine (Human) Antibody Array includes 80 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888€
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AA0084 Cytokine (Mouse) Antibody Array includes 34 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0084 Cytokine (Mouse) Antibody Array includes 34 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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bs-1276R-FITC Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, FITC conjugated,Isotype: IgG Sepax 100ug Lyophilized 304 bs-1276R-FITC Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, FITC conjugated,Isotype: IgG Sepax 100ug Lyophilized 304€ Pub
bs-7653R-PE-Cy3 Rabbit Anti-DNase gamma Polyclonal Antibody, PE-Cy3 Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530 bs-7653R-PE-Cy3 Rabbit Anti-DNase gamma Polyclonal Antibody, PE-Cy3 Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530€ Pub
AA0052 Cytokine (Human) Antibody Array (G) includes 51 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888 AA0052 Cytokine (Human) Antibody Array (G) includes 51 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888€
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E-3101-2 includes 10 x reaction buffer with MgCl2 Bioneer 2000 Units 345 E-3101-2 includes 10 x reaction buffer with MgCl2 Bioneer 2000 Units 345€ Pub
bs-1359R Rabbit Anti-DNAPK PRKDC Polyclonal Antibody, Unconjugated Bioss 100ug 330 bs-1359R Rabbit Anti-DNAPK PRKDC Polyclonal Antibody, Unconjugated Bioss 100ug 330€ Pub
AA0019 Chromatin Transcription Phospho-Specific Array includes 173 highly specific and well-characterized phosphorylation antibodies in the Chromatin Transcription pathway. Abnova 2 Pieces/Box 822 AA0019 Chromatin Transcription Phospho-Specific Array includes 173 highly specific and well-characterized phosphorylation antibodies in the Chromatin Transcription pathway. Abnova 2 Pieces/Box 822€
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AA0029 Insulin Receptor Phospho-Specific Array includes 219 highly specific and well-characterized phosphorylation antibodies in the insulin receptor pathway. Abnova 2 Pieces/Box 822 AA0029 Insulin Receptor Phospho-Specific Array includes 219 highly specific and well-characterized phosphorylation antibodies in the insulin receptor pathway. Abnova 2 Pieces/Box 822€
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AA0093 Inflammation (Mouse) Antibody Array includes 40 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0093 Inflammation (Mouse) Antibody Array includes 40 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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228-10329-2 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 10 205 228-10329-2 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 10 205€
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02-050-5 T4 DNA Ligase, standard kit B-Bridge 5 X 20,000 U 257.25 02-050-5 T4 DNA Ligase, standard kit B-Bridge 5 X 20,000 U 257.25€
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S2025-D-B-230V* ProBlot Rocker 25D with double platform (26 x 20 cm), 230V*, includes EU and UK power cords Labnet International Inc. 690 S2025-D-B-230V* ProBlot Rocker 25D with double platform (26 x 20 cm), 230V*, includes EU and UK power cords Labnet International Inc. 690€
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AA0061 Inflammation (Human) Antibody Array (G) includes 40 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0061 Inflammation (Human) Antibody Array (G) includes 40 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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G2100 DNAfectin 2100 (A) ABM Goods 1ml; 100-500 transfections 135 G2100 DNAfectin 2100 (A) ABM Goods 1ml; 100-500 transfections 135€ Pub
228-10337-1 Recombinant Human DNase Proteins Ray Biotech 500IU 147 228-10337-1 Recombinant Human DNase Proteins Ray Biotech 500IU 147€
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bs-7653R Rabbit Anti-DNase gamma Polyclonal Antibody Sepax 100ug Lyophilized 222 bs-7653R Rabbit Anti-DNase gamma Polyclonal Antibody Sepax 100ug Lyophilized 222€ Pub
AA0038 p53 Signaling Phospho-Specific Array includes 196 highly specific and well-characterized phosphorylation antibodies in the p53 signaling pathway. Abnova 2 Pieces/Box 822 AA0038 p53 Signaling Phospho-Specific Array includes 196 highly specific and well-characterized phosphorylation antibodies in the p53 signaling pathway. Abnova 2 Pieces/Box 822€
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C2400-230V* Spectrafuge 24D digital microcentrifuge, standard gray, 230V*, includes EU and UK power cords Labnet International Inc. 1213 C2400-230V* Spectrafuge 24D digital microcentrifuge, standard gray, 230V*, includes EU and UK power cords Labnet International Inc. 1213€
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228-10332-2 Recombinant Human HSP70 DnaK Proteins Ray Biotech 50 205 228-10332-2 Recombinant Human HSP70 DnaK Proteins Ray Biotech 50 205€
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4250-050-ESK CometAssay Electrophoresis Starter Kit (includes CometAssay Kit, Control Cells & ES) Trevigen - 2112 4250-050-ESK CometAssay Electrophoresis Starter Kit (includes CometAssay Kit, Control Cells & ES) Trevigen - 2112€ Pub
AA0070 Atherosclerosis (Mouse) Antibody Array includes 22 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0070 Atherosclerosis (Mouse) Antibody Array includes 22 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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22331 DNAJA2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329 22331 DNAJA2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329€
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bs-1276R-Biotin Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Biotin Conjugated Bioss 100ug 369 bs-1276R-Biotin Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Biotin Conjugated Bioss 100ug 369€ Pub
bs-7653R-Cy7 Rabbit Anti-DNase gamma Polyclonal Antibody, Cy7 conjugated Isotype: IgG Sepax 100ug Lyophilized 268 bs-7653R-Cy7 Rabbit Anti-DNase gamma Polyclonal Antibody, Cy7 conjugated Isotype: IgG Sepax 100ug Lyophilized 268€ Pub
AA0047 Cytokine (Human) Antibody Array (G) includes 42 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0047 Cytokine (Human) Antibody Array (G) includes 42 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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E-3100-1 includes 10 mM dNTP, 10 x reaction buffer, 20mM MgCl2 Bioneer 500 Units 167 E-3100-1 includes 10 mM dNTP, 10 x reaction buffer, 20mM MgCl2 Bioneer 500 Units 167€ Pub
228-10335-2 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 100 205 228-10335-2 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 100 205€
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AA0014 AMPK Signaling Phospho-Specific Array includes 174 highly specific and well-characterized phosphorylation antibodies in the AMPK signaling pathway. Abnova 2 Pieces/Box 822 AA0014 AMPK Signaling Phospho-Specific Array includes 174 highly specific and well-characterized phosphorylation antibodies in the AMPK signaling pathway. Abnova 2 Pieces/Box 822€
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AA0079 Cytokine (Human) Antibody Array includes 51 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888 AA0079 Cytokine (Human) Antibody Array includes 51 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888€
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AA0088 Cytokine (Rat) Antibody Array includes 34 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0088 Cytokine (Rat) Antibody Array includes 34 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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bs-1276R-PE-Cy5 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy5 Conjugated Bioss 100ug 402 bs-1276R-PE-Cy5 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy5 Conjugated Bioss 100ug 402€ Pub
DS-PB-00617 Rabbit Anti-Bovine DNAse 1 Antibodies Ray Biotech 1 ml 485 DS-PB-00617 Rabbit Anti-Bovine DNAse 1 Antibodies Ray Biotech 1 ml 485€
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AA0056 Cytokine (Mouse) Antibody Array (G) includes 34 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0056 Cytokine (Mouse) Antibody Array (G) includes 34 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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E-3121 Includes 5 X reaction buffer, 100 mM DTT Bioneer 10000 Units 128 E-3121 Includes 5 X reaction buffer, 100 mM DTT Bioneer 10000 Units 128€ Pub
000493A DNASE I ABM Goods 250ul 621 000493A DNASE I ABM Goods 250ul 621€
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AA0023 EGF Phospho-Specific Array includes 214 highly specific and well-characterized phosphorylation antibodies related to the EGF family of proteins. Abnova 2 Pieces/Box 822 AA0023 EGF Phospho-Specific Array includes 214 highly specific and well-characterized phosphorylation antibodies related to the EGF family of proteins. Abnova 2 Pieces/Box 822€
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3214-100 DNase I Antibody Biovision 100 301 3214-100 DNase I Antibody Biovision 100 301€
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AA0033 MAPK Phospho-Specific Array includes 185 highly specific and well-characterized phosphorylation antibodies in the MAPK pathway. Abnova 2 Pieces/Box 822 AA0033 MAPK Phospho-Specific Array includes 185 highly specific and well-characterized phosphorylation antibodies in the MAPK pathway. Abnova 2 Pieces/Box 822€
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BB24-AU-CE Homogenizer for 24 samples in microcentrifuge tubes. Large enclosure to help reduce noise and active cooling to maintain 4C interior temperature. Includes starter kit of beads, scoops and tubes. Up Next Advence 4850 BB24-AU-CE Homogenizer for 24 samples in microcentrifuge tubes. Large enclosure to help reduce noise and active cooling to maintain 4C interior temperature. Includes starter kit of beads, scoops and tubes. Up Next Advence 4850€ Pub
228-10330-3 Recombinant E. coli HSP70 DnaK Proteins Ray Biotech 1mg 1658 228-10330-3 Recombinant E. coli HSP70 DnaK Proteins Ray Biotech 1mg 1658€ Pub
22940 DNA ligase 3 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329 22940 DNA ligase 3 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329€
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Y050476 Anti-ACSL3(long-chain fatty-acid-Coenzyme A ligase 3) Antibody ABM Goods 100ug 386.4 Y050476 Anti-ACSL3(long-chain fatty-acid-Coenzyme A ligase 3) Antibody ABM Goods 100ug 386.4€
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AA0065 Angiogenesis (Human) Antibody Array includes 20 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0065 Angiogenesis (Human) Antibody Array includes 20 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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228-10327-3 Recombinant E. coli HSP40 DnaJ Proteins Ray Biotech 1mg 1031 228-10327-3 Recombinant E. coli HSP40 DnaJ Proteins Ray Biotech 1mg 1031€ Pub
bs-1276R Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Unconjugated Bioss 100ug 330 bs-1276R Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Unconjugated Bioss 100ug 330€ Pub
bs-7653R-A647 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323 bs-7653R-A647 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323€ Pub
AA0042 Tyrosine Kinase Adaptors Phospho-Specific Array includes 98 highly specific and well-characterized phosphorylation antibodies related to Tyrosine Kinase Adaptors. Abnova 2 Pieces/Box 731 AA0042 Tyrosine Kinase Adaptors Phospho-Specific Array includes 98 highly specific and well-characterized phosphorylation antibodies related to Tyrosine Kinase Adaptors. Abnova 2 Pieces/Box 731€
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228-10333-3 Recombinant E. coli HSP70 DnaK LCS Proteins Ray Biotech 1mg 1031 228-10333-3 Recombinant E. coli HSP70 DnaK LCS Proteins Ray Biotech 1mg 1031€
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AA0004 Cell cycle antibody array includes 60 highly specific and well-characterized antibodies in the cell cycle pathway. Abnova 2 Pieces/Box 731 AA0004 Cell cycle antibody array includes 60 highly specific and well-characterized antibodies in the cell cycle pathway. Abnova 2 Pieces/Box 731€
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AA0074 Cytokine (Human) Antibody Array includes 36 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0074 Cytokine (Human) Antibody Array includes 36 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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AA0083 Cytokine (Mouse) Antibody Array includes 62 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888 AA0083 Cytokine (Mouse) Antibody Array includes 62 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888€
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bs-1276R-Cy7 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Cy7 Conjugated Bioss 100ug 369 bs-1276R-Cy7 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Cy7 Conjugated Bioss 100ug 369€ Pub
bs-7653R-PE Rabbit Anti-DNase gamma Polyclonal Antibody, PE Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502 bs-7653R-PE Rabbit Anti-DNase gamma Polyclonal Antibody, PE Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502€ Pub
AA0051 Cytokine (Human) Antibody Array (G) includes 54 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888 AA0051 Cytokine (Human) Antibody Array (G) includes 54 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888€
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hAP-0248 Mouse anti human DNAM-1 Anti-Human antibodies AngoiPro 100.00 ug 310 hAP-0248 Mouse anti human DNAM-1 Anti-Human antibodies AngoiPro 100.00 ug 310€ Pub
AA0018 Cell Cycle Phospho-Specific Array includes 76 highly specific and well-characterized phosphorylation antibodies in the Cell Cycle pathway. Abnova 2 Pieces/Box 731 AA0018 Cell Cycle Phospho-Specific Array includes 76 highly specific and well-characterized phosphorylation antibodies in the Cell Cycle pathway. Abnova 2 Pieces/Box 731€
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AA0092 Inflammation (Human) Antibody Array includes 40 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0092 Inflammation (Human) Antibody Array includes 40 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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228-10329-1 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 2 147 228-10329-1 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 2 147€
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02-050 T4 DNA Ligase, standard kit T4 DNA Ligase, standard kit B-Bridge 20000 U 50.4 02-050 T4 DNA Ligase, standard kit T4 DNA Ligase, standard kit B-Bridge 20000 U 50.4€
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RT013 dNTPs Smart Serology 10mM, 200pl 154 RT013 dNTPs Smart Serology 10mM, 200pl 154€
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AA0060 Cytokine (Rat) Antibody Array (G) includes 34 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0060 Cytokine (Rat) Antibody Array (G) includes 34 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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228-10336-3 Native Bacterial DNase Proteins Ray Biotech 1g 733 228-10336-3 Native Bacterial DNase Proteins Ray Biotech 1g 733€
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AA0028 IGF-1R Signaling Phospho-Specific Array includes 245 highly specific and well-characterized phosphorylation antibodies in the insulin-like growth factor 1 receptor signaling pathway. Abnova 2 Pieces/Box 822 AA0028 IGF-1R Signaling Phospho-Specific Array includes 245 highly specific and well-characterized phosphorylation antibodies in the insulin-like growth factor 1 receptor signaling pathway. Abnova 2 Pieces/Box 822€
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BAH67-1000 Protease, DNASE free heat shock BSA powder, 1KG Equitech 1KG 772.8 BAH67-1000 Protease, DNASE free heat shock BSA powder, 1KG Equitech 1KG 772.8€
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AA0037 Nuclear Membrane Receptors Phospho-Specific Array includes 52 highly specific and well-characterized phosphorylation antibodies related to Nuclear Membrane Receptors. Abnova 2 Pieces/Box 731 AA0037 Nuclear Membrane Receptors Phospho-Specific Array includes 52 highly specific and well-characterized phosphorylation antibodies related to Nuclear Membrane Receptors. Abnova 2 Pieces/Box 731€
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BIO-27026 T4 DNA Ligase Bioline 500 Units (weiss) 90.7673 BIO-27026 T4 DNA Ligase Bioline 500 Units (weiss) 90.7673€ Pub
228-10332-1 Recombinant Human HSP70 DnaK Proteins Ray Biotech 10 147 228-10332-1 Recombinant Human HSP70 DnaK Proteins Ray Biotech 10 147€
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298-65103 Single Strand DNA Ligase, thermostable, recombinant, Solution Single Strand DNA Ligase, thermostable, recombinant, Solution B-Bridge 200 units 573.3 298-65103 Single Strand DNA Ligase, thermostable, recombinant, Solution Single Strand DNA Ligase, thermostable, recombinant, Solution B-Bridge 200 units 573.3€
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AA0069 Atherosclerosis (Human) Antibody Array includes 20 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0069 Atherosclerosis (Human) Antibody Array includes 20 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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DS-MB-03301 Mouse Anti-Human HSP40 DnaJ Antibodies Ray Biotech 0.1 mg 452 DS-MB-03301 Mouse Anti-Human HSP40 DnaJ Antibodies Ray Biotech 0.1 mg 452€
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bs-1276R-A647 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323 bs-1276R-A647 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323€ Pub
bs-7653R-Cy5.5 Rabbit Anti-DNase gamma Polyclonal Antibody, Cy5.5 conjugated, Isotype: IgG Sepax 100ug Lyophilized 268 bs-7653R-Cy5.5 Rabbit Anti-DNase gamma Polyclonal Antibody, Cy5.5 conjugated, Isotype: IgG Sepax 100ug Lyophilized 268€
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AA0046 Apoptosis (Human) Antibody Array (G) includes 43 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0046 Apoptosis (Human) Antibody Array (G) includes 43 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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E-3100 includes 10 mM dNTP, 10 x reaction buffer with 20 mM MgCl2 Bioneer 500 Units 138 E-3100 includes 10 mM dNTP, 10 x reaction buffer with 20 mM MgCl2 Bioneer 500 Units 138€ Pub
228-10335-1 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 20 147 228-10335-1 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 20 147€
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AA0013 AKT PKB Signaling Phospho-Specific Array includes 216 highly specific and well-characterized phosphorylation antibodies in the AKT PKB signaling pathway. Abnova 2 Pieces/Box 822 AA0013 AKT PKB Signaling Phospho-Specific Array includes 216 highly specific and well-characterized phosphorylation antibodies in the AKT PKB signaling pathway. Abnova 2 Pieces/Box 822€
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AA0078 Cytokine (Human) Antibody Array includes 54 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888 AA0078 Cytokine (Human) Antibody Array includes 54 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 888€
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AA0087 Cytokine (Rat) Antibody Array includes 19 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0087 Cytokine (Rat) Antibody Array includes 19 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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bs-1276R-PE-Cy3 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy3 Conjugated Bioss 100ug 401 bs-1276R-PE-Cy3 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy3 Conjugated Bioss 100ug 401€ Pub
bs-7653R-PE-Cy7 Rabbit Anti-DNase gamma Polyclonal Antibody, PE-Cy7 conjugated Isotype: IgG Sepax 100ug Lyophilized 300 bs-7653R-PE-Cy7 Rabbit Anti-DNase gamma Polyclonal Antibody, PE-Cy7 conjugated Isotype: IgG Sepax 100ug Lyophilized 300€ Pub
AA0055 Cytokine (Mouse) Antibody Array (G) includes 62 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888 AA0055 Cytokine (Mouse) Antibody Array (G) includes 62 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 888€
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BAH67-0500 Protease, DNASE free heat shock BSA powder, 500gm Equitech 440 BAH67-0500 Protease, DNASE free heat shock BSA powder, 500gm Equitech 440€
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AA0022 Cytoskeleton II Phospho-Specific Array includes 141 highly specific and well-characterized phosphorylation antibodies in the cytoskeletal pathway. Abnova 2 Pieces/Box 776 AA0022 Cytoskeleton II Phospho-Specific Array includes 141 highly specific and well-characterized phosphorylation antibodies in the cytoskeletal pathway. Abnova 2 Pieces/Box 776€
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24046 Primary antibody DNase II Antibody, Source Rabbit SignalWay Antibody S.A.B 100ug 364 24046 Primary antibody DNase II Antibody, Source Rabbit SignalWay Antibody S.A.B 100ug 364€
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AA0032 Jak Stat II Phospho-Specific Array includes 202 highly specific and well-characterized phosphorylation antibodies in the Jak Stat pathway. Abnova 2 Pieces/Box 822 AA0032 Jak Stat II Phospho-Specific Array includes 202 highly specific and well-characterized phosphorylation antibodies in the Jak Stat pathway. Abnova 2 Pieces/Box 822€
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AA0096 Th1 Th2 Th17 (Human) Antibody Array includes 34 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0096 Th1 Th2 Th17 (Human) Antibody Array includes 34 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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228-10330-2 Recombinant E. coli HSP70 DnaK Proteins Ray Biotech 50 205 228-10330-2 Recombinant E. coli HSP70 DnaK Proteins Ray Biotech 50 205€ Pub
22508 DNA ligase 3 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC IF SignalWay Antibody S.A.B 100ul 329 22508 DNA ligase 3 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC IF SignalWay Antibody S.A.B 100ul 329€
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U1000-230V* DyNA Light UV Transilluminator, 21 x 26 cm viewing area, 230V* includes EU and UK power cords Labnet International Inc. 1355 U1000-230V* DyNA Light UV Transilluminator, 21 x 26 cm viewing area, 230V* includes EU and UK power cords Labnet International Inc. 1355€
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AA0064 Th1 Th2 Th17 (Human) Antibody Array (G) includes 34 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731 AA0064 Th1 Th2 Th17 (Human) Antibody Array (G) includes 34 highly specific and well-characterized antibodies on glass slide. Abnova 4 Arrays/Slide 731€
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228-10327-2 Recombinant E. coli HSP40 DnaJ Proteins Ray Biotech 100 205 228-10327-2 Recombinant E. coli HSP40 DnaJ Proteins Ray Biotech 100 205€ Pub
ER-14-0857 Goat Anti-Human MDG1 DNAJB9, (C Terminus) Antibodies Ray Biotech 100 μg 353 ER-14-0857 Goat Anti-Human MDG1 DNAJB9, (C Terminus) Antibodies Ray Biotech 100 μg 353€
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bs-7653R-A555 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 555 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323 bs-7653R-A555 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 555 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323€ Pub
AA0041 TGF-Beta Signaling Phospho-Specific Array includes 176 highly specific and well-characterized phosphorylation antibodies in the TGF-Beta signaling pathway. Abnova 2 Pieces/Box 822 AA0041 TGF-Beta Signaling Phospho-Specific Array includes 176 highly specific and well-characterized phosphorylation antibodies in the TGF-Beta signaling pathway. Abnova 2 Pieces/Box 822€
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E-2016 Pfu DNA Polymerase (Not available for sale in USA) Without dNTPs, 10 x reaction buffer Bioneer 1000 Units 485 E-2016 Pfu DNA Polymerase (Not available for sale in USA) Without dNTPs, 10 x reaction buffer Bioneer 1000 Units 485€ Pub
228-10333-2 Recombinant E. coli HSP70 DnaK LCS Proteins Ray Biotech 100 205 228-10333-2 Recombinant E. coli HSP70 DnaK LCS Proteins Ray Biotech 100 205€
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AA0002 Apoptosis antibody array includes 73 highly specific and well-characterized antibodies in the apoptosis pathway. Abnova 2 Pieces/Box 731 AA0002 Apoptosis antibody array includes 73 highly specific and well-characterized antibodies in the apoptosis pathway. Abnova 2 Pieces/Box 731€
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AA0073 Cytokine (Human) Antibody Array includes 42 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731 AA0073 Cytokine (Human) Antibody Array includes 42 highly specific and well-characterized antibodies on membrane. Abnova 4 Membranes/Box 731€
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