Product name :T-cell proliferation grade human type II collagen, 0.5 mg

Catalog Number :2025

Quantity :

Price :362.25 €

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Supplier :Chondrex

DESCRIPTION: Highly purified by CM-cellulose chromatography, heat denatured type II collagen
APPLICATION: Use as a stimulation antigen for T-cells from experimental animals and humans in vitro culture system, for example, T-cell proliferation assay and establishing type II collagen specific T-cell lines.
QUANTITY: 0.5 mg
FORM: Lyophilized powder
SOURCE: Human sternal cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
SOLUBILITY: Soluble in acidic and neutral buffers, and in cell culture medium. In order to use this product for assaying T-cell proliferation responses, it is recommended to dissolve this product in PBS rather than cell culture media at 1 mg/mL before use.
STORAGE TEMPERATURE: 4°C in the dark. This product is stable at 4°C for 2 years as supplied. Denatured collagen dissolved in acidic solution is also stable at -20°C for 1 year, but will gradually degrade under neutral conditions.
STABILITY: 2 years

 

T-cells play a crucial role in the induction of collagen-induced arthritis (CIA), since type II collagen is a T-cell dependent antigen. However, it is difficult to study T-cell responses to type II collagen even in CIA high responder animals by T-cell proliferation assay using spleen and lymph node cells due to several reasons: 1) Collagen is not a strong T-cell antigen and T-cell population specific to collagen is low. 2) T-cell proliferation responses will be masked due to the high background levels caused by polyclonally stimulated B-cells with adjuvant. B-cells, even from animals immunized with incomplete adjuvant, proliferate spontaneously. 3) Macrophages activated by adjuvant components might release a large amount of immuno-suppresive cytokines, which inhibit T-cell proliferation. 4) Pepsin, which is commonly used for solubilizing collagen and common contaminant in collagen preparations, is a strong T-cell antigen, and creates significantly high false positive results. In order to determine true T-cell responses to collagen, it is important to use a T-cell enriched fraction from spleen or lymph node cells, and to use highly purified, pepsin free collagen preparations as a T-cell stimulation antigen in vivo.


Type II collagen is particularly used as an immunizing antigen for inducing arthritis in rodents and monkeys. However, depending upon the research purposes, animals may be immunized with type I collagen or receive collagen implants. Chondrex provides various species of highly purified T-cell grade type I collagen as well as type II collagen for various purposes of immunological studies on collagen and collagen related materials (i.e. T-cell proliferation assays and establishing T-cell lines and T-cell hybridoma cells). T-cell grade type I and type II collagen (0.5 mg/vial, lyophilized) is supplied in a heat-denatured form. In addition, for T-cell epitope studies CNBr-digested type II collagen fragments can be provided upon request. Type I collagen can be used as a control antigen for T-cell epitope studies in the CIA model and other autoimmune models, since type I and type II collagen share more than 80% amino acid sequences, but their immunological features differ significantly.

 

PROCEDURE FOR in vitro t-cell proliferation assay


Immunization
Immunize animals such as mice, rats or monkeys with type II collagen emulsified with CFA (catalog # 7001) or IFA (catalog # 7002). Follow the immunization protocol for inducing collagen-induced arthritis in individual animals. Collect spleen or lymph node cells on day 8-12 after immunization. In the case of monkeys, use peripheral blood mononuclear cells or an underarm lymph node removed surgically.
Note 1: It is not necessary to use the immunization protocol for inducing CIA in these animals, but it might be convenient to follow the protocol.
For example, immunize mice with 100 mg of type II collagen emulsified with CFA, rats with 200 mg of type II collagen emulsified in IFA and monkeys with 250 mg of type II collagen emulsified with CFA.
Note 2: T-cell proliferation response and their epitope specificity are not dependent on the conformational structure of type II collagen or the type of adjuvant. Identical T-cell responses are observed in mice immunized with either native or denatured collagen emulsified with both CFA and IFA. Therefore, for T-cell studies, denatured collagen and even CB peptide fragments can be used as immunizing antigens. On the other hand, antibody specificity and induction of arthritis are strongly dependent on the conformational structure of type II collagen and the type of adjuvant (see “Successful Induction of Arthritis in Mice” and “Successful Induction of Arthritis in Rats”).

Purification of T-Cells
1. Pack 0.6 g of pre-washed nylon-wool into a 10 mL syringe, soak with PBS and autoclave for 20 minutes. Replace the PBS with Dulbecco’s Minimum Essential Medium (DMEM) supplemented with 1% homologous normal serum, penicillin-streptomycin (1X), and 2-mercaptoethanol (2-ME - 5 x 10-5M). This is called complete DMEM.
Note: Do not use fetal calf or bovine serum in any case, since T-cells spontaneously react to heterologous serum components. In the case of monkeys, use Protein A-adsorbed autologous serum or plasma (Protein A removes immunoglobulins including antibodies against collagen).
2. Prepare a single cell suspension from spleen or lymph node cells from mice immunized with type II collagen (or type I collagen), wash 3 times with DMEM and then suspend the cells in 1-2 mL of complete DMEM (approximately 1 x 108 cells/mL).
3. Load the cell suspension onto the column. Incubate the column for 30 minutes in a CO2 incubator at 37°C. Elute unbound cells with 20 mL DMEM and then centrifuge at 800 rpm for 8 minutes. Suspend the enriched T-cells at 4-8 x 106 cells/mL in complete DMEM.


Preparation of Antigen Presentation Cells (APC)
1. Use spleen or thymus cells obtained from young, normal congenic animals (6-8 weeks old) as APC. Wash the cells with DMEM 3 times and suspend at 1 x 107 cells/mL in complete DMEM.
Note: In the case of monkeys, use Mytomycin C (an inhibitor of DNA synthesis) treated peripheral blood mononuclear cells (PBMC) isolated by the Ficoll-Hypaque method as APC.
2. Irradiate the cells with 2000 rad = 20 Gy.
Note: Do not disturb the cells after irradiation and treatment with Mytomycin C (50 mg/mL for 1 hour).


T-Cell Proliferation Assay
1. Dissolve T-cell grade type II collagen (or type I collagen) at 1 mg/mL with warm PBS or DMEM and then centrifuge at 10,000 rpm for 3 minutes at room temperature. Transfer the supernatant to a sterile tube and store at -20°C until needed.
Note: T-cell grade type II collagen (or type I collagen) has been denatured by heating. Type II collagen (or type I collagen) used for T-cell stimulation should be denatured, since APC are not capable of processing native type II collagen (or type I collagen) in the short culture period.
2. Add 0.1 mL of enriched T-cells (4-8 x 106 cells/mL), 0.1 mL of APC (1 x 107 cells/mL) and 10-20 mL of 1 mg/mL T-cell grade type II collagen (or type I collagen) in a 96-well flat bottom plate. Culture the cells in a CO2 incubator at 37°C with 5% CO2 for 3 days.
Note 1: In the case of monkeys, optimization of T-cells/APC ratio (in general, an equal number of T-cell and APC recommended) is critical to obtain the maximum T-cell responses to collagen.
Note 2: Do not use round bottom plates.
3. Add 3H-labeled thymidine (0.5-1.0 mCi/well) on day 3 and culture for an additional 15-18 hours. Harvest the cells on a glass filter and assay for the incorporated 3H by scintillation counting.

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20352 Mouse anti-human type II collagen IgG2a antibody subtype assay kit, OPD Chondrex 442.05€
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20032 IMPII SOL Immunization grade porcine type II collagen, 10 mg solution Chondrex 285.60€
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1003 ELPI ELISA grade porcine type I collagen, 0.5 mg Chondrex 157.50€
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1033T Human monkey anti-porcine type I collagen IgG antibody assay kit, TMB Chondrex 528.36€
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1041 Human monkey anti-chick type I collagen IgA antibody assay kit, OPD Chondrex 528.36€
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2017 ELMkII ELISA grade monkey type II collagen, 0.5 mg Chondrex 304.50€
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20062 Immunization grade mouse type II collagen, 1 mg solution Chondrex 203.70€
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1081 Immunization grade chick type XI collagen, 5 mg Chondrex 276.15€
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MD-14-0836 Mouse Anti-Human Collagen Type I Antibodies Ray Biotech 100 452.00€
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2055 Human monkey anti-human type II collagen IgG antibody assay kit, OPD Chondrex 588.42€
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20021 IMBII Immunization grade bovine type II collagen, 10 mg lyophilized Chondrex 285.60€
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20353T Mouse anti-human type II collagen IgG2b antibody subtype assay kit, TMB Chondrex 442.05€
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2012 ELISA grade bovine type II collagen, 0.5 mg solution Chondrex 207.90€
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1015 ELMGHI Mouse IgG anti human type I collagen ELISA kit Chondrex 529.20€
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20042 IMRII SOL Immunization grade rat type II collagen Chondrex 5 mg solution 354.90€
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1066 Immunization grade mouse type I collagen, 5 mg Chondrex 220.50€
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1071 Immunization grade chick type IX collagen, 5 mg Chondrex 325.50€
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1045 ELHAHI Human Monkey IgA anti human type I collagen ELISA kit Chondrex 559.65€
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2062 Human monkey anti-bovine type II collagen IgA antibody assay kit, OPD Chondrex 518.81€
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2035T Mouse anti-human type II collagen IgG antibody assay kit, TMB Chondrex 434.70€
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1001 ELISA grade chick type I collagen, 0.5 mg Chondrex 130.20€
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1062 Immunization grade bovine type I collagen, 10 mg Chondrex 169.05€
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1031T Human monkey anti-chick type I collagen IgG antibody assay kit, TMB Chondrex 528.36€
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2014 ELISA grade rat type II collagen, 0.5 mg solution Chondrex 241.50€
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1072 Immunization grade bovine type IX collagen, HM & LM, 5 mg lyophilized Chondrex 402.15€
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2052 Human monkey anti-bovine type II collagen IgG antibody assay kit, OPD Chondrex 518.81€
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20012 IMCII SOL Immunization grade chick type II collagen Chondrex 10 mg solution 226.80€
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20351T Mouse anti-human type II collagen IgG1 antibody subtype assay kit, TMB Chondrex 442.05€
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20032 IMPII SOL Immunization grade porcine type II collagen Chondrex 10 mg solution 226.80€
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1003 ELPI ELISA grade porcine type I collagen Chondrex 0.5 mg 133.35€
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1064 IMRI Immunization grade rat type I collagen, 5 mg lyophilized Chondrex 270.90€
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1033 Human monkey anti-porcine type I collagen IgG antibody assay kit, OPD Chondrex 528.36€
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21675 C EBPβ Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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Y104931 Brain-Specific Angiogenesis Inhibitor 1 (BAI1), Human Antibody ABM Goods 50ug 1 041.00€
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DS-MB-01036 Mouse Anti-Human Collagen II Antibodies Ray Biotech 0.1 ml 535.00€ Pub
BC04118 Lung squamous cell carcinoma and adjacent normal tissue array, including TNM, clinical stage and pathology grade, 50 cases 100 cores US Biomax 231.00€
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21613 Glycogen Synthase Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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DS-MB-03292 Mouse Anti-Human Fibroblasts Epithelial cells Antibodies Ray Biotech 25 180.00€ Pub
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IPLA-N-50ml-LiHeparin Pooled Normal Human Plasma 50ml Li Heparin Innovative Research INC 107.00€
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2044 ELRGRII Rat IgG anti rat type II collagen ELISA kit Chondrex 541.80€
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E-3061 Includes 10 X reaction buffer, 100mM MgCl2, 50mM DTT, 10mM ATP, 25 ug mL BSA Bioneer 20000 Units 90.00€ Pub
21469 CD34 Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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bs-6850R-PE Rabbit Anti-TPST1 Polyclonal Antibody, PE Conjugated , 42-50kDa; Isotype IgG; Reactivity Human , Mouse , Bovine , Dog; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
CBEL-ERK-SK Human Mouse Rat Phospho-ERK1 2, JNK, p38 MAPK Cell-Based ELISA ELISA Ray Biotech Two 96-Well Microplate Kit 607.00€
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20-273 Agar, Powdered Bacto-Style Bacteriological Grade Genesee 500g/Unit 99.00€
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20333 Mouse anti porcine type II collagen IgG2b assay kit Chondrex 497.70€
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010-14531 Anti C Reactive Protein Anti Human C Reactive Protein (CRP) monoclonal antibody is produced by hybridoma clone 409 being established by fusing myeloma cells NS 1 and spleen cells of BALB c mice which B-Bridge 1mg 800.10€
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YSRTBUF012B Alamar Blue™, REDOX indicator dye, for quantitative measurement of cell proliferation Accurate 100 ml. 450.63€
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cAP-0004RFP RFP Expressing Human Glomerular Microvascular Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
Y105153 G Protein Coupled Receptor GPR92 GPR93, Human ABM Goods 50ug 1 174.00€
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11189 Ephrin-B2 (Phospho-Tyr330) Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 224.00€
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228-10246-3 Recombinant Human CKMM Type-1 Proteins Ray Biotech 1mg 997.00€
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137-13614 Macrophage Colony Stimulating Factor (M CSF), Human, recombinant Macrophage colony stimulating factor, or M CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into m B-Bridge 1 mg 0.00€
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21334 SLK1 Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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21631 VEGFR1 Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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BS02031 Esophagus squamous cell carcinoma (grade II) tissue array, 22 cases 63 cores, replaced by ES723 US Biomax 160.00€
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22274 MGC16169 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC IF SignalWay Antibody S.A.B 100ul 329.00€
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IPLA-SER-500ml Pooled Normal Human Serum 500ml Innovative Research INC 430.00€ Pub
1021T Rat anti-chick type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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MC243 Multiple cancer (3 types) tissue array, 24 cases 24 cores, with grade and stage data US Biomax 100.00€
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21497 ASH2L Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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CMA241 Human tumor cell array, 12 cell lines 24 cores US Biomax 278.00€
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IC05-3061-50ml Rat Red Blood Cells 50ml Innovative Research INC 50ml 339.00€ Pub
KD486 Kidney clear cell carcinoma with matched adjacent kidney tissue array, including TNM and pathology grade, 16 cases 48 cores US Biomax 225.00€
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cAP-0013 Human Tonsil Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
IRAPKT027 Human Apolipoprotein CII ELISA Kit (Cell culture samples) Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
DS-PB-00527 Goat Anti-Human Collagen VI Antibodies Ray Biotech 0.2 mg 766.00€ Pub
CSB-E09530h Human anti-parainfluenza virus IgM antibody, anti-PIV IgM ELISA Kit, Species Human, Sample Type serum, plasma Cusabio 96T 816.00€
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Y105269 G Protein Coupled Receptor MRGX2 (MRGPRX2), Human ABM Goods 50ug 789.00€
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11536 Stat2 (phospho-Tyr690) Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 224.00€
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42-653 Competent Cells for CloningGC5 Competent Cells, pUC19 Genesee 75 x 50ul/Unit 704.00€
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20313 Mouse anti-chick type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 400.05€
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21456 PKM1 2 Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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BAH63-0500 Standard grade heat shock BSA powder, pH 5.2, 500gm Equitech 310.00€
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11009 PTEN(Phospho-Ser380) Antibody, Rabbit Polyconal Ab, Human Mouse Rat SignalWay Antibody S.A.B 50ul 190.00€
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LC808 Lung squamous cell carcinoma tissue array, with TNM, stage and grade, 80 cases 80 cores, replacing BS04011 US Biomax 231.00€
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bs-2123R-PE Rabbit Anti-Cyp2-j3 Polyclonal Antibody, PE Conjugated , 55kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , Horse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
21582 Beclin-1 Antibody, Rabbit Polyconal Ab, Species Reactivity: Human SignalWay Antibody S.A.B 50ul 186.00€
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MNO961 35 types of normal human tissue array, 90 cases 96 cores US Biomax 225.00€
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1013T Mouse anti-porcine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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IRB-N-500ml-NaHeparin Rabbit Plasma US Origin Innovative Grade™ 500ml Na Heparin Innovative Research INC 864.00€
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RD172022100 Human, Clara Cell Protein, E.coli , Rec. Prot. Biovendor 0.1 mg 352.00€ Pub
ES802 Esophageal squamous cell carcinoma tissue array, grade I III, 40 cases 80 cores, no overlaps with ES801 US Biomax 231.00€
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20363 Mouse anti mouse type II collagen IgG2b assay kit Chondrex 603.75€
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23159 mitochondrial carrier protein MGC4399 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329.00€
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ZRD-TGFB1-025 Stem Cell Products TGF-b1, human , 25 ug SBI 25 ug 1 438.00€
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M1228 Octyl â D 1 thioglucopyranoside, A non ionic detergent for purification of soluble proteins from cells, including bacteria, and for solubilization and reconstitution of membrane proteins, 500mg MarkerGene 103.00€
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CSB-E17034h Human Helicobacter pylori antibody(IgA) ELISA Kit, Species Human, Sample Type serum, plasma Cusabio 96T 913.00€
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20323 Mouse anti bovine type II collagen IgG2b assay kit Chondrex 497.70€
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IRAPKT158 Human Protein C ELSIA Kit (Urine & Cell Culture Samples) Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
20325T Mouse anti-bovine type II collagen IgG2b antibody subtype assay kit, TMB Chondrex 400.05€
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21684 Smad1 Smad5 Smad8 Antibody, Rabbit Polyconal Ab, Species Reactivity: Human Mouse Rat SignalWay Antibody S.A.B 50ul 186.00€
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bs-7599R-PE-Cy3 Rabbit Anti-STK3 Polyclonal Antibody, PE-Cy3 Conjugated , 56kDa; Isotype IgG; Reactivity Mouse , Human; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
cAP-0001 Human Umbilical Vein Endothelial Cells AngoiPro 1.00 flask 206.00€ Pub
Y105142 G Protein Coupled Receptor GPR62, Human ABM Goods 50ug 1 174.00€
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228-10243-1 Recombinant Human CKMB Type-1 Proteins Ray Biotech 10 147.00€
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22943 B-cell linker protein antibody Source Rabbit Polyconal Ab Species Human Application WB IF SignalWay Antibody S.A.B 100ul 329.00€
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21618 Neuroserpin Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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Y055266 Purified Rabbit Anti Human BIN3 Polyclonal ABM Goods 50ug 514.00€
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FDA808a-2 Multiple organ tumor tissue array with adjacent normal human organs, including TNM, clinical stage and pathology grade, 72 cases 72 cores, replacing FDA808-2; ihc Anti-Actin confirmed US Biomax 267.00€
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BAC61-0500 Fraction V Microbiological grade BSA powder, 500gm Equitech 500gm 495.96€
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21480 ERG Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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IPLA-CSER-50ml Pooled Complement Human Serum 50ml Innovative Research INC 50ml 306.00€ Pub
CBEL-STAT3-1 Human Mouse Rat Phospho-Stat 3 (Y705) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347.00€ Pub
20335T Mouse anti-porcine type II collagen IgG2c antibody subtype assay kit, TMB Chondrex 400.05€
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cAP-0007GFP GFP Expressing Human Coronary Artery Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
Y105158 G Protein Coupled Receptor GPR52, Human ABM Goods 50ug 1 174.00€
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11330 α-catenin (Phospho-Ser641) Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 224.00€
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MD-11-0034 Mouse Anti-Human CD19 (pan B-cells) Antibodies Ray Biotech 200 361.00€
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21431 TFE3 Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 195.00€
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CQLD-100 QuantiChrom™ LDH Cytotoxicity Assay Kit, Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure Bioassays 100tests 113.00€ Pub
1022T Rat anti-bovine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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228-10497-3 Native Human FSH Proteins Ray Biotech 5000IU 485.00€ Pub
MC483 Multiple types of cancer tissue array with normal control, replacing BC00119, 26 cases 48 cores, with stage and grade data US Biomax 160.00€
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BAH68-0500 Diagnostic grade heat shock BSA powder, 500gm Equitech 500gm 557.66€
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21555 hnRNP A1 (Ab-192) Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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504-A anti CD7 All T cells Recognition human CD7 antigen Clone ICO 87 Virogen 100 ug/vial 225.00€
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ES244 Esophageal squamous cell carcinoma tissue array with matched adjacent esophageal tissue, including TNM and pathology grade, 12 cases 24 cores; ihc Anti-Actin confirmed US Biomax 90.00€
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SPR-312A Recombinant Human Aha2 Stressmarq 50ug 227.00€
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cAP-0018 Human Internal Mammary Artery Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
11574 β-catenin (phospho-Tyr333) Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 224.00€
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20315 Mouse anti-chick type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 400.05€
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21658 HNF-1β(TCF-2) Antibody  Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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C340 Recombinant Human Endothelial Protein C Receptor EPCR, Source: HEK293 Novoprotein 500ug 1 451.00€
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Y104914 G Protein Coupled Receptor L1 G10D (ADMR), Human ABM Goods 50ug 1 174.00€
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11016 NFκB-p100 p52(Phospho-Ser870) Antibody, Rabbit Polyconal Ab, Human Mouse Rat SignalWay Antibody S.A.B 50ul 190.00€
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228-10704-2 Recombinant Human HGF [from CHO cells] Proteins Ray Biotech 10 205.00€ Pub
MD-14-0791 Mouse Anti-Human CD41 Platelet gpIIb IIIa ITGA2B Antibodies Ray Biotech 100 292.00€
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21602 ERAB Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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100-04 Human Stem Cell Factor SCF Shenandoah Biotechnology, Inc. 2ug 113.40€ Pub
1016T Mouse anti-mouse type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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IRB-N-50ml-NaEDTA Rabbit Plasma US Origin Innovative Grade™ 50ml Na EDTA Innovative Research INC 185.00€
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1085-100 Active Human Caspase 5100 units Biovision 100 units 295.79€
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ES961 Esophagus squamous cell carcinoma tissue array, including TNM and pathology grade, 48 cases 96 cores US Biomax 336.00€
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20365T Mouse anti-mouse type II collagen IgG2c antibody subtype assay kit, TMB Chondrex 485.10€
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bs-7687R-PE Rabbit Anti-LAP3 Polyclonal Antibody, PE Conjugated , 56kDa; Isotype IgG; Reactivity Human , Rat , Mouse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€
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IPLA-N-500ml-NaEDTA Pooled Normal Human Plasma 500ml Na EDTA Innovative Research INC 430.00€
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Y102937 Apolipoprotein E (APO E), Human ABM Goods 50ug 928.20€
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DAG434 Recombinant Human Immunodeficiency Virus Type 1, Gp120, GST-His Tagged CDiagnostic 1mg 580.00€
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DS-PB-01377 Goat Anti-Human MSR Type I Antibodies Ray Biotech 0.1 ml 411.00€
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LC2081 Multiple lung carcinoma (Non small cell lung carcinoma, NSCLC) and normal tissue form necroscopy tissue array, 70 cases 208 cores, with stage and grade information US Biomax 456.00€
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2042 ELRGBII Rat IgG anti bovine type II collagen ELISA kit Chondrex 477.75€
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HS-0500 Human serum off the clot, unfiltered, 500ml Equitech 500ml 495.96€ Pub
IGLYHPAI-A-0.5mg Human PAI-1 (glycosylated wild type) 0.5mg Innovative Research INC 1 162.00€
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Y055626 Purified Rabbit Anti Human DCLRE1C Polyclonal Antibody ABM Goods 50ug 455.00€
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cAP-18 Bovine Type I Collagen based Hydrogel AngoiPro 50.00 ml 215.00€ Pub
DS-MB-01204 Mouse Anti-Human Endothelial Cells Antibodies Ray Biotech 0.5 ml 766.00€
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M1475 Marker Gene™ Non-Radioactive Cell Proliferation Assay Kit (MTT Assay Kit), Quickly and accurately measures cell viability, 1 kit MarkerGene 341.00€
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20331 Mouse anti porcine type II collagen IgG1 assay kit Chondrex 497.70€
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R9012HR-500g HyAgarose™ HR Agarose, PCR Grade, Green Agarose Product, 500g ACTGene 500g 863.00€
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cAP-0002 Human Brain Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
100-35 Human Tumor Necrosis Factor Receptor Type 1 TNF-Receptor-1 Shenandoah Biotechnology, Inc. 5ug 113.40€ Pub
IR100044P Biological Fluids: Pooled Normal Human Saliva Innovative Research INC 50ml 395.00€
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DS-PB-00503 Rabbit Anti-Human Collagen II Antibodies Ray Biotech 0.5 ml 568.00€ Pub
Y105147 G Protein Coupled Receptor 37 Like 1 (GPR37L1), Human ABM Goods 50ug 1 174.00€
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11091 Src(Phospho-Tyr418) Antibody, Source: Rabbit Polyconal Ab, Species reactivity: Human Mouse Rat SignalWay Antibody S.A.B 50ul 190.00€
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228-10244-3 Recombinant Human CKMB Type-2 Proteins Ray Biotech 1mg 997.00€
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133-13611 Macrophage Colony Stimulating Factor (M CSF), Human, recombinant Macrophage colony stimulating factor, or M CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into ma B-Bridge 10 ug 520.80€ Pub
21208 FAK (Ab-397) Antibody Source Rabbit Polyconal Ab Species Human Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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21624 GAD67(GAD1) Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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bs-7700R-PE Rabbit Anti-BTN2A2 Polyclonal Antibody, PE Conjugated , 55kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
21488 Vimentin Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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KD244 Kidney clear cell carcinoma tissue array with matched kidney adjacent tissue, including TNM and pathology grade, 12 cases 24 cores; ihc Anti-Actin confirmed US Biomax 90.00€
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cAP-0010GFP GFP Expressing Human Retinal Microvascular Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
Y105165 G Protein Coupled Receptor GPR160 GPCR150, Human ABM Goods 50ug 1 174.00€
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20311 Mouse anti-chick type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 400.05€
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21438 SYT Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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bs-6352R-PE Rabbit Anti-Adenylosuccinate Lyase Polyclonal Antibody, PE Conjugated , 55kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
3458-024-K Cultrex 24 Well Collagen IV Cell Invasion Assay Trevigen 24 tests 431.00€ Pub
21640 MBP(myelin basic protein) Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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BAH62-0050 Standard grade heat shock BSA powder, pH 7, 50gm Equitech 154.00€
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100-159 Human Stromal Cell-Derived Factor-1 beta (CXCL12) SDF-1 β CXCL12 Shenandoah Biotechnology, Inc. 2ug 113.40€
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1023T Rat anti-porcine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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SJA01-04 Recombinant Human GM-CSF Cytokin. 50μg/vial 655.00€
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21572 CK2α Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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228-11175-2 Recombinant Human OPG TNFRSF11b [from Hi-5 cells] Proteins Ray Biotech 10 205.00€
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1012 ELMGBI Mouse IgG anti bovine type I collagen ELISA kit Chondrex 464.10€
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CSB-EQ027313HU Human phosphatidyl ethanol(PEth) ELISA kit, Species , Sample Type Cusabio 96T 816.00€ Pub
228-11623-3 Recombinant Human VEGF VEGFA [from CHO cells] Proteins Ray Biotech 1mg 0.00€
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20361 Mouse anti mouse type II collagen IgG1 assay kit Chondrex 603.75€ Pub
cAP-0020 Human Iliac Artery Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
11581 P38 MAPK(Phospho-Thr180 Tyr182) Antibody , Rabbit Polyconal Ab, Species Reactivity: Human Mouse Rat SignalWay Antibody S.A.B 50ul 214.00€
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K301-500 Quick Cell Proliferation Assay Kit Biovision 500 assays 230.00€ Pub
228-10266-1 Recombinant Human Collagen-3 Ray Biotech 10 mg 449.00€ Pub
M082329 Monoclonal Mouse Anti Human CD31 Endothelial Cell, Clone JC70A Dako 0.2 mL 303.45€ Pub
20321 Mouse anti-bovine type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 503.00€
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bs-6770R-PE-Cy3 Rabbit Anti-DRAK1 STK17A Polyclonal Antibody, PE-Cy3 Conjugated , 50kDa; Isotype IgG; Reactivity Mouse , Rat , Human; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
21666 CCR5(CD195) Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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22141 Type II 5-phosphatase antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329.00€
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GAHMFC-0500 Goat Anti Human IgM (FC), 500ml Equitech 500ml 571.89€
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Y050801 Anti-APOA5(Apolipoprotein A5)(Pair to Human) Antibody ABM Goods 50ug 386.40€
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3001 Rapid collagenase assay kit with type I collagen substrate Chondrex 454.65€
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21607 FoxA2(HNF3β) Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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IR-HU-GF-50mg Human IgG 50mg Innovative Research INC 50mg 264.00€ Pub
Y104930 Bombesin Receptor Subtype 3 (BRS3), Human Antibody ABM Goods 50ug 1 041.00€
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DS-MB-01035 Mouse Anti-Human Collagen I II III Antibodies Ray Biotech 1 ml 361.00€
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21612 GAPDH Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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IPLA-N-50ml-K3EDTA Pooled Normal Human Plasma 50ml K3 EDTA Innovative Research INC 107.00€
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Y102966 C MYC, Human ABM Goods 500ug 1 942.00€
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BAB100-0050 Borellia grade BSA powder, Borrelia and Leptospira growth tested, 50gm Equitech 50gm 297.81€
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2043T Rat anti-porcine type II collagen IgG antibody assay kit, TMB Chondrex 384.30€
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228-10941-3 Recombinant Human IL-6 (IL-6) [from CHO cells] Proteins Ray Biotech 1mg 0.00€ Pub
CBEL-ERK-1 Human Mouse Rat Phospho-ERK1 2 (T202 Y204) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347.00€ Pub
20333 Mouse anti-porcine type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 400.05€
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YSRTBUF012A Alamar Blue™, REDOX indicator dye, for quantitative measurement of cell proliferation Accurate 25 ml. 218.16€
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cAP-0004GFP GFP Expressing Human Glomerular Microvascular Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
CSB-E04776h Human Squamous Cell Carcinoma Antigen 2,SCCAg 2 ELISA Kit Cusabio 96tests 816.90€
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Y105152 G Protein Coupled Receptor GPR1, Human ABM Goods 50ug 1 174.00€
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11188 Ephrin-B2 (Phospho-Tyr316) Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 224.00€
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228-10246-2 Recombinant Human CKMM Type-1 Proteins Ray Biotech 50 205.00€
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BC07115 Kidney clear cell carcinoma tissue array, including TNM and pathology grade, 120 cases 120 cores US Biomax 321.00€
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21330 α-catenin (Ab-641) Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 195.00€
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21630 Doublecortin Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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BS02021 Esophagus squamous cell carcinoma (grade I) tissue array, 21 cases 63 cores US Biomax 160.00€
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BAH64-0050 Reagent grade heat shock BSA powder, 50gm Equitech 50gm 279.30€
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MD-14-0099 Mouse Anti-Human Proliferating Cell Nuclear Antigen (PCNA) Antibodies Ray Biotech 100 292.00€
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CM481 Common 4 types of cancer tissue array, 48 cases 54 cores, with stage and grade data US Biomax 231.00€
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CSB-EL022397PL Human superoxide dismutase 1, soluble (SOD1) ELISA kit, Species , Sample Type Cusabio 96T 816.00€
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IC05-3054-50ml Mouse Red Blood Cells 50ml Innovative Research INC 50ml 339.00€ Pub
SPR-309A Recombinant Human Ahsa1 Stressmarq 50ug 227.00€
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cAP-0012RFP RFP Expressing Human Liver Sinusoidal Microvascular Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
DS-PB-00526 Rabbit Anti-Human Collagen V Antibodies Ray Biotech 0.5 ml 568.00€
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CSB-E09381h Human squamous cell carcinoma related antigen,SCCAg ELISA Kit Cusabio 96tests 913.50€
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Y105173 G Protein Coupled Receptor GPR18, Human ABM Goods 50ug 1 174.00€
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11531 VEGFR2 (phospho-Tyr1059) Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 224.00€
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A822850 1-Azido-3-chloro-2-propanol, Technical Grade C3H6ClN3O CAS: 51275-91-3 TRC 250 mg 245.00€
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42-652 Competent Cells for CloningGC5 Competent Cells, pUC19 Genesee 20 x 50ul/Unit 231.00€
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MD-11-0080 Mouse Anti-Human CD49b Collagen Receptor Antibodies Ray Biotech 200 535.00€
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20312T Mouse anti-chick type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 400.05€
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21451 Periostin Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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21645 γ-Catenin Antibody Source Rabbit Polyconal Ab Species Human Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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T-1313.0500 C Peptide of Proinsulin (human) Clone C PEP 01 Bachem 500µg 567.00€
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21579 Smad1 (Ab-206) Antibody, Rabbit Polyconal Ab, Species Reactivity: Human Mouse Rat SignalWay Antibody S.A.B 50ul 186.00€
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IRB-N-500ml-NaEDTA Rabbit Plasma US Origin Innovative Grade™ 500ml Na EDTA Innovative Research INC 864.00€
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LC10012 Lung non small cell cancer tissue array, with adjacent tissue and normal tissue, including TNM and pathology grade, 50 cases 100 cores, replacing LC1006 US Biomax 266.00€
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20363 Mouse anti-mouse type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 485.10€
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228-10806-3 Recombinant Human T-cell Leukemia Virus 1 & 2 (HTLV-1 + HTLV-2) Ray Biotech 1 mg 4 059.00€ Pub
ZRD-TGFB1-005 Stem Cell Products TGF-b1, human , 5 ug SBI 5 ug 414.00€
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IRAPKT068 Human Kininogen (HMW) ELISA Kit (Urine, Milk, Saliva & Cell Culture Samples) Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
20323 Mouse anti-bovine type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 400.05€
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21674 Oct-1 Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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bs-7596R-PE-Cy3 Rabbit Anti-RIP3 Polyclonal Antibody, PE-Cy3 Conjugated , 57kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
20325 Mouse anti-bovine type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 400.05€
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21681 IRF-3 (Ab-396) Antibody, Rabbit Polyconal Ab, Species Reactivity: Human SignalWay Antibody S.A.B 50ul 186.00€
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bs-7599R-PE Rabbit Anti-STK3 Polyclonal Antibody, PE Conjugated , 56kDa; Isotype IgG; Reactivity Mouse , Human; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
IR100007-50ml Normal Human Urine 50ml Innovative Research INC 138.00€ Pub
Y105141 G Protein Coupled Receptor Kinase 5 (GRK5), Human ABM Goods 50ug 1 174.00€
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DS-MB-01042 Mouse Anti-Human Collagen VII Antibodies Ray Biotech 2 ml 411.00€ Pub
21617 Tubulin beta-III Antibody Source Rabbit Polyconal Ab Species Human Mouse Application WB SignalWay Antibody S.A.B 50ul 195.00€
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LC2085b High density (208 cores), multiple types of lung cancer (grade 1 3) tissue array with normal lung and cancer adjacent tissue, and staging info, 188 cases 208 cores (duplicate of LC2085a) US Biomax 456.00€
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TP483 Top 4 types of cancer (colon, breast, prostate and lung) tissue microarray, 48 case 48 cores, with grade, Gleason score and stage data US Biomax 160.00€
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21479 CDCP1(CD318) Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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CBEL-STAT1-1 Human Mouse Phospho-Stat 1 (Y701) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347.00€ Pub
20335 Mouse anti-porcine type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 400.05€
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cAP-0007 Human Coronary Artery Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
Y105157 G Protein Coupled Receptor GPR52, Human ABM Goods 50ug 1 174.00€
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11241 SP1 (Phospho-Thr739) Antibody Source Rabbit Polyconal Ab Species Human Rat Application WB SignalWay Antibody S.A.B 50ul 224.00€
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RF0014-50 IgG Rabbit Polyclonal to Human HGH (Somatotropin) Argenvec 50ìg 201.71€
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DS-MB-01673 Mouse Anti-Human IIDO Antibodies Ray Biotech 0.1 mg 766.00€ Pub
MD-11-0033 Mouse Anti-Human CD19 (pan B-cells) Antibodies Ray Biotech 1 mL 568.00€
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T047 Non-small cell lung cancer test tissue array (4x (2x3)array) including TNM, clinical stage and pathology grade, 6 cases 24 cores; ihc Anti-Actin confirmed US Biomax 90.00€
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23083 killer cell lectin-like receptor subfamily C, member 4 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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21430 ASPSCR1 Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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21635 TIF1β Antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 50ul 195.00€
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BAB100-0500 Borellia grade BSA powder, Borellia and Leptospira growth tested, 500gm Equitech 490.44€
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BS04071 Lung squamous cell carcinoma (grade III) tissue array, 21 cases 63 cores, replaced by LC723 US Biomax 160.00€
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SHSABC-0500 Sterile filtered human AB serum off the clot, 500ml Equitech 500ml 618.45€
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228-10497-2 Native Human FSH Proteins Ray Biotech 500IU 318.00€ Pub
MC482 Multiple cancer (12 type) tissue microarray, 40 cases 48 cores, with stage and grade information US Biomax 160.00€
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21547 ZAP70(Ab-292) Antibody, Rabbit Polyconal Ab, Species Reactivity: Human Mouse Rat SignalWay Antibody S.A.B 50ul 186.00€
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MD-16-0008P Native Human CA15-3, Antigen grade Antibodies Ray Biotech 250 KU 1 312.00€
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cAP-0017 Human Large Intestine Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
228-11222-3 Recombinant Human PEDF [from HEK cells] Proteins Ray Biotech 100 1 172.00€ Pub
CSB-E11715h Human Iinterleukin 25,IL-25 ELISA Kit SpeciesHuman Cusabio 96T 599.00€ Pub
11572 CK2α (phospho-Thr360 Ser362) Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 224.00€
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20314T Mouse anti-chick type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 402.15€
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Y051663 Anti Angiotensin II Receptor Type 1 (AT1 Receptor) (extracellular) ABM Goods 50μl 745.50€
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21656 β-Enolase(ENO-3) Antibody  Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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BAH64-0500 Reagent grade heat shock BSA powder, 500gm Equitech 336.00€
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Y104913 G Protein Coupled Receptor L1 G10D (ADMR), Human ABM Goods 50ug 1 174.00€
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11015 NFκB-p100 p52(Phospho-Ser866) Antibody, Source: Rabbit Polyconal Ab, Species reactivity: Human Mouse Rat SignalWay Antibody S.A.B 50ul 190.00€
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GAHGFC-0500 Goat Anti Human IgG (FC), 500ml Equitech 500ml 635.96€
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228-10704-1 Recombinant Human HGF [from CHO cells] Proteins Ray Biotech 2 147.00€ Pub
MD-14-0790 Mouse Anti-Human CD40 (CD40+ B-cells) Antibodies Ray Biotech 200 535.00€
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E01P0004 Human Anti-Parainfluenza virus 1-5 type IgM ELISA Kit BlueGen 96 Tests/kit 1 087.00€ Pub
21601 EEA1 Antibody Source Rabbit Polyconal Ab Species Human Mouse Rat Application WB SignalWay Antibody S.A.B 50ul 195.00€
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CO2085b TMA BLOCK of high density (208 cases 208 cores), multiple type colon adenocarcinoma (I 4 grade) with normal colon from autopsy and cancer adjacent tissue US Biomax 40 981.00€
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1016 Mouse anti-mouse type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
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IRB-N-50ml-NaCitrate Rabbit Plasma US Origin Innovative Grade™ 50ml Na Citrate Innovative Research INC 185.00€
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LC1009 Lung small cell carcinoma tissue array for antibody screening, including TNM, clinical stage and pathology grade, 25 cases 100 cores US Biomax 231.00€
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20365 Mouse anti-mouse type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 485.10€
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60501 Wnt Signaling Pathway TCF LEF reporter-HEK293 stable cell line BPS Bioscience 1.5x10(6) cells 6 420.00€
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LC1922 Middle advanced stage lung non small cell carcinoma tissue array, including TNM and pathology grade, 96 cases 192 cores US Biomax 456.00€
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2041T Rat anti-chick type II collagen IgG antibody assay kit, TMB Chondrex 384.30€
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23167 fibronectin type III and SPRY domain containing 2 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329.00€
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MD-22-0016 Native Human Collagen V Antibodies Ray Biotech 100 1 137.00€
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Y055626 Purified Rabbit Anti Human DCLRE1C Polyclonal ABM Goods 50ug 514.00€
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