Product name : Rapid Microplate Assay Kit for PDH Activity and Quantity (Combo)

Catalog Number :MSP20

Quantity : 96 Tests

Price :988.05 €

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MSP20 Rapid Microplate Assay Kit for PDH Activity and Quantity (Combo)


MitoProfile® Rapid Microplate Assay Kit for PDH Activity and Quantity (Combines Kit MSP18 & MSP19)
Cat. No.MSP20


DESCRIPTION
MitoProfile® Rapid Microplate Assay Kit PDH Activity (MSP18) and PDH Quantity (MSP19) Kit Contents: Included in this kit is the necessary buffer (Tube 1), Detergent for sample preparation, and Reagent Mix for the reaction. The kit contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.


Storage: Tube 1, Detergent and the covered microplate should be stored at 4°C. For multiple experiments the two supplied Reagent Mix tubes must be further aliquoted and stored at -80°C. For more details on aliquoting and storage, see frequently asked questions.
INTRODUCTION The Rapid Microplate Assay for PDH Activity (MSP18) is used to determine the activity of pyruvate dehydrogenase (PDH) in a sample. The PDH enzyme is immunocaptured within the wells of the microplate and activity is determined by following the reduction of NAD+ to NADH with an increase in the absorbance at 340 nm.

The supplied activity Reagent Mix contains the 5 substrates as noted in the reaction scheme figure. While PDH activity can be regulated by PDH kinase and PDH phosphatase this kit does not include PDH kinase or PDH phosphatase inhibitors. These may be incorporated into the sample preparation and/or assay buffers at the researcher’s discretion. This rapid PDH microplate has been developed for use with human samples. Bovine, mouse, or rat materials are also compatible. Other species have not been tested. It is recommended to prepare a crude mitochondrial sample; for details see frequently asked questions section. It is also possible to use whole tissue lysates but may require some sample optimization. The protein concentration of the sample should be measured. Once diluted to the specified concentration the sample is detergent solubilized and diluted to within the linear range of measurement. A control or normal sample should always be included in the assay as a reference measurement. In addition a buffer control should be used as a negative control. Table 1. Typical ranges of measurement:
Typical intra-assay variations (same day, same sample) <10% The protocol has 4 steps: A) Sample preparation Note - methods for mitochondria OR cultured cells are described B) Plate loading C) Measurement. D) Data Analysis


ADDITIONAL MATERIALS REQUIRED
Spectrophotometer measuring absorbance of 340 nm Deionized water

Multichannel Pipetting devices Protein assay method Phosphate buffered saline solution (PBS)
1.4 mM KH2PO4
8 mM Na2HPO4
140 mM NaCl
2.7 mM KCl, pH 7.3


MICROPLATE ASSAY PROTOCOL
A. Sample Preparation Method #1 – Tissue Mitochondria
1. Determine the sample protein concentration using a standard method such as BCA method (Pierce). Adjust the concentration of the sample to 5 mg/mL using PBS.
For details of sample solubilization conditions see frequently asked questions section.
2. Extract the proteins by adding Detergent. To do this add 1/10 of a volume of Detergent (e.g. if the total sample volume is 500 μL, add 50 μL of Detergent). Mix well. Place the tube in a rack at room temperature for 10 minutes to allow solubilization.
3. Add contents of Tube 1 (10 mL) to 190 mL deionized H2O. Label this mixture as Solution 1. Mix.
4. Dilute all samples to the desired concentration in Solution 1. Table 1 above shows a good linear range for the assay, the table below shows a recommended mid-range value at which to load an experimental sample.


A. Sample Preparation Method #2 – Cultured cell extract
1. Resuspend the cell pellet in 0.1 mL PBS per plate of confluent cells. Determine the sample protein concentration using a standard method such as BCA method (Thermo Fisher - Pierce). Adjust the concentration of the sample to 5 mg/mL using PBS. Approximate numbers of cells per mg of protein are given in the frequently asked questions section.
2. Extract the proteins by adding Detergent. To do this add 1/10 of a volume of Detergent (e.g. if the total sample volume is 200 μL, add 20 μL of Detergent). Mix well. Place the tube in a rack at room temperature for 10 minutes to allow solubilization.
3. Add contents of Tube 1 (10 mL) to 190 mL deionized H2O. Label this mixture as Solution 1. Mix.
4. Dilute all samples to the desired concentration in Solution 1. Table 1 above shows a good linear range for the assay, the table below shows a recommended mid-range value at which to load an experimental sample.


Recommended sample dilutions:

B. Plate Loading
1. Add 200 μL of sample prepared in Section A4 to each well of the microplate that will be used for this experiment. Be sure to include a normal or control sample in addition to a buffer control as described in the introduction.
2. Incubate microplate for 3 hours at room temperature.
C. Measurement For multiple experiments the Reagent Mix should be aliquoted and stored at -80°C according to frequently asked questions.
1. Thaw Reagent Mix.
2. Empty or aspirate the wells of the microplate (this can be accomplished by turning the plate over, shaking out the liquid, and blotting on paper towel to complete) and to each well add 300 μL of Solution 1.
3. Make Assay Solution (1.75 mL/strip) according to the following table:
4. Empty the wells of the microplate.
5. Rinse all wells once more with 300 μL Solution 1.
6. Empty the wells again.
7. Add 200 μL of Assay Solution to each well carefully, to avoid bubbles. Any bubbles should be popped with a fine needle as rapidly as possible.
8. Measure the absorbance of each well at 340 nm at room temperature using a kinetic program for at least 60 minutes. The interval between readings should be as short as your reader allows but not longer than 5 minutes between reads. Incorporate a shake step between reads if possible.
D. Data Analysis PDH activity is expressed as the initial rate of reaction, determined from the slopes of the curves generated (for example see Figure 1 below). Using the plate reader software or a spreadsheet application such as Microsoft Excel, the linear portion of the initial rate can be determined. Figure 1, an example of microplate reader recorded data:
This activity should always be related to a control or normal sample to obtain the relative activity of PDH in experimental samples. Figure 2 below shows an example of the activity of PDH captured from a bovine heart mitochondrial detergent extract. The sample was diluted to show that over this range of concentrations the signal is linear. Each sample was measured in triplicate and the low background signal was subtracted.

FLOW CHART (For quick reference only. Be completely familiar with previous details of this document before performing the assay.)

Frequently asked questions
How should I store my kit?

Tube 1, the covered microplate and the detergent should be refrigerated and are stable for at least 6 months. Once Tube 1 is diluted 20X to make Solution 1 it must be used in the assay at room temperature. It can also be stored at room temperature, however for extended periods of time (>1 month) it should be stored in the refrigerator to prevent microbiological growth.


How should I store my reagent mix?

Reagent mix is temperature sensitive. It must be stored at -80°C and is stable for at least six months from date of receipt. Because it is frozen it is also advisable to aliquot the reagent mix before freezing to prevent repeated freeze thaw cycles.


How should I aliquot my reagent mix?

Divide the reagent mix into equal aliquots depending on how many experiments you wish to run (consult table step C3). The plate comes in 12 strips, therefore it is anticipated that up to 12 experiments on different days could be done. The reagent mix is supplied as 2 x 0.5 mL aliquots so for 12 independent experiments you could further aliquot each tube into 6 x 83 μL aliquots and immediately freeze at -80°C for storage until use.

How do I optimize sample solubilization?

Mitochondria have two membrane bilayers which must be dissolved by detergent before PDH can be isolated. PDH is a huge, extremely complex, multisubunit enzyme which is sensitive to high detergent concentrations. Therefore the correct detergent:protein ratio must be used to efficiently solubilize the mitochondrial outer and inner membranes while maintaining PDH integrity. This kit contains an optimized ratio, however this ratio may need to be adjusted from sample to sample particularly when contaminated with excess extracellular proteins such as serum. Therefore, it may be necessary to adjust the concentration of detergent or isolate mitochondria.


How do I make mitochondria?

We have found that little or no optimization is necessary if crude mitochondria are made from samples. Mitochondria can be prepared by simple differential centrifugation of homogenized samples as described in our mitochondrial preparation kit handbook: http://www.mitosciences.com/isolation_kits.html


How do I grow and prepare cultured cell samples? PDH activity in cells from different origins differs greatly. Cells grown in glucose have a lower activity than those grown in galactose/glutamine. Consequently cell type and growth conditions are a large factor in PDH activity measured.
Approximately how much protein is yielded from my plate of cells? We find the following typical yield of cells from a single confluent 177 cm2 plate:
It is recommended that you accurately determine from your first confluent plate the number of cells and the total protein yield.


DESCRIPTION

MitoProfile® Rapid Microplate Assay Kit PDH Quantity (MSP19) Kit Contents: Included in this kit is the necessary buffer (Tube 1), a microplate pre-coated with a PDH binding antibody, detergent for sample preparation, antibodies for PDH detection (Tubes A & B), and substrates for an alkaline phosphatase reaction (Tubes 2 & 3). The kit contains enough materials to perform 96 tests. Since the plate is arranged as 12 strips of 8 wells it is suggested that up to 12 separate experiments can be performed.

Storage: Tube 1, Tube A, Tube B, Tube 2, Detergent and the covered microplate should be stored at 4°C. Tube 3 must be stored at -20°C or -80°C and for multiple experiments may first be aliquoted. For more details on aliquoting and storage see frequently asked questions. When stored correctly the kit is stable for 6 months.


INTRODUCTION The Rapid Microplate Assay Kit for PDH Quantity (MSP19) is used to determine the amount of pyruvate dehydrogenase in a sample. This assay is a ‘sandwich’ ELISA, where the PDH enzyme is captured by an antibody on the wells of the microplate. The amount of captured enzyme is determined by adding another PDH specific antibody which is then labeled with the enzyme alkaline phosphatase (AP). This phosphatase changes the substrate pNPP from colorless to yellow (OD 405 nm) in a time dependent manner which is proportional to the amount of protein captured in the wells. This rapid PDH microplate has been developed for use with human samples but bovine, mouse, and rat materials are also compatible. Other species have not been tested. It is also possible to use whole tissue or cell lysate without the need for mitochondrial isolation. The protein concentration of the sample should be measured. Once diluted to the specified concentration the sample is detergent solubilized and diluted to within the linear range of measurement. A control or normal sample should always be included in the assay as a reference measurement. In addition a buffer control should be used as a negative control. Typical ranges of measurement:

Typical intra-assay variations (same day, same sample) <15% The protocol has 4 steps: A) Sample preparation B) Plate loading C) Antibody addition D) Measurement and data analysis


ADDITIONAL MATERIALS REQUIRED
Spectrophotometer measuring absorbance at 405 nm (alternatively 450 nm is acceptable) Deionized water Multichannel Pipetting devices Protein assay method Phosphate buffered saline solution (PBS)
1.4 mM KH2PO,4 8 mM Na2HPO4, 140 mM NaCl, 2.7 mM KCl, pH 7.3


MICROPLATE ASSAY PROTOCOL
A. Sample Preparation Method #1 – Tissue Mitochondria
1. Determine the sample protein concentration using a standard method such as BCA method (ThermoFisher - Pierce). Adjust the concentration of the sample to 5 mg/mL using PBS.
For details of sample solubilization conditions see frequently asked questions section.
2. Extract the proteins by adding Detergent. To do this add 1/10 of a volume of Detergent (e.g. if the total sample volume is 500 μL, add 50 μL of Detergent). Mix well. Place the tube in a rack at room temperature for 10 minutes to allow solubilization.
3. Add contents of Tube 1 (15 mL) to 285 mL deionized H2O. Label this mixture as Solution 1. Mix.
4. Dilute all samples to the desired concentration in Solution 1. The table above shows a good linear range for the assay, the table below shows a recommended mid-range value at which to load an experimental sample.


A. Sample Preparation Method #2 – Cultured cell extract
1. Resuspend the cell pellet in 0.1 mL PBS per plate of confluent cells. Determine the sample protein concentration using a standard method such as BCA method (ThermoFisher - Pierce).
Adjust the concentration of the sample to 5 mg/mL using PBS. Approximate numbers of cells per mg of protein are given in the frequently asked questions section.
2. Extract the proteins by adding Detergent. To do this add 1/10 of a volume of Detergent (e.g. if the total sample volume is 200 μL, add 20 μL of Detergent). Mix well. Place the tube in a rack at room temperature for 10 minutes to allow solubilization.
3. Add contents of Tube 1 (15 mL) to 285 mL deionized H2O. Label this mixture as Solution 1. Mix.
4. Dilute all samples to the desired concentration in Solution 1. The table above shows a good linear range for the assay, the table below shows a recommended mid-range value at which to load an experimental sample.
Recommended sample dilutions:
B. Plate Loading
3. Add 200 μL of sample prepared in Section A4 to each well of the microplate that will be used for this experiment. Be sure to include a normal or control sample in addition to a buffer control as described in the introduction.
4. Incubate microplate for 3 hours at room temperature.
C. Antibody Addition For multiple experiments the Tube 3 should be aliquoted and stored at -20°C/-80°C according to frequently asked questions.
1. The bound monoclonal antibody has immobilized the enzyme in the wells. Empty/aspirate the wells (this can be accomplished by turning the plate over and shaking out the liquid. Blot on paper towel to complete aspiration).
2. Add 300 μL of Solution 1 to each well.
3. Add 1 mL antibody from Tube A to 20 mL of Solution 1. Label as Solution A.
If using only a portion of the strips make only a proportionate amount of solution A.
4. Empty/aspirate the wells and add 200 μL of Solution A to each well used. Incubate 1 hour at room temperature.
5. Empty/aspirate the wells and add 300 μL of Solution 1 to each well.
6. Add 1 mL antibody Tube B to 20 mL of Solution 1. Label as Solution B.
Again, if using only a portion of the strips make only a proportionate amount of Solution B.
7. Empty/aspirate the wells and add 200 μL of Solution B to each well used. Incubate 1 hour at room temperature.
8. Empty/aspirate the wells and add 300 μL of Solution 1 to each well used. Repeat this twice more.
9. Add the 0.4 mL from Tube 3 to 20 mL from Tube 2. Label as Development Solution.
If using only a portion of the strips make only a proportionate amount of development solution. See frequently asked questions for examples.
10. Empty/aspirate the wells and add 200 μL of development solution to each well used.
11. Measure the absorbance of each well at 405 nm (or 450 nm) at room temperature using a kinetic program for at least 30 minutes. The interval between readings should be as short as your reader allows but not longer than 2 minutes between reads. Incorporate a shake step between reads if possible.
An endpoint measurement at 405 nm or 450 nm is also possible at a time defined by the user when a yellow color development in specific wells is acceptable.
D. Data Analysis Examine the color development over time in each well. Under the conditions stated above the color development should be linear over the 30 minute time period of measurement. Subtract the initial absorbance reading from the final absorbance reading to determine the quantity of PDH in each well. This quantity should always be related to a control or normal sample to obtain the relative quantity of PDH in experimental samples. Below is an example of the quantity of PDH capture from a fibroblast cell lysate. The sample was diluted to show that over this range of concentrations the signal is linear. Each sample was measured in triplicate and the low background signal was subtracted.

FLOW CHART (For quick reference only. Be completely familiar with previous details of this document before performing the assay.)


Frequently asked questions

How should I store my kit?

Tube 3 should be stored frozen at -20°C or preferably -80°C and is stable for 6 months. Tube 3 can first be aliquoted if desired to prevent freeze thaw cycling. All other components in the kit should be stored at 4°C in a refrigerator. Once Tube 1 is diluted 20X to make Solution 1 it can be stored at room temperature, however for extended periods of time (>1 month) it should be stored in the refrigerator to prevent microbiological growth.

How should I aliquot my Tube 3?

Divide the Tube 3 into equal aliquots depending on how many experiments you wish to run (consult table below). The plate comes in 12 strips, therefore it is anticipated that up to 12 experiments on different days could be done. Tube 3 is supplied as 0.4 mL aliquots so for 12 independent experiments you could aliquot the tube into 12 x 33 μL aliquots and immediately freeze until use. How much Development Buffer should I prepare? Depending on how many strips you are using prepare the following :

How do I grow and prepare cultured cell samples?

The amount of PDH in cells from different origins differs greatly. Cells grown in glucose have a lower activity than those grown in galactose/glutamine. Consequently cell type and growth conditions are a large factor in PDH activity measured. Approximately how much protein is yielded from my plate of cells? We find the following typical yield of cells from a single confluent 177 cm2 plate:
It is recommended that you accurately determine from your first confluent plate the number of cells and the total protein yield.
 

datasheet of product : Rapid Microplate Assay Kit for PDH Activity and Quantity (Combo)  Rapid Microplate Assay Kit for PDH Activity and Quantity (Combo)

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22803 Cell Meter™ NIR Mitochondria Membrane Potential Assay Kit *Optimized for Microplate Reader* AAT Bioquest 1 kit 231.00€
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Pub
K145-100 Cathepsin H Activity Assay Kit Biovision 100 assays 357.00€
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Pub
K781-100 Protease Activity Assay Kit Biovision 100 assays 289.00€ Pub
CBA-200 Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay) Cell Biolabs 50 assays 407.00€
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Pub
K430-40 KinaseSTAR JNK Activity Screening Kit Biovision 40 assays 559.00€ Pub
K265-200 Hydrogen Peroxide Assay Kit200 assays Biovision 200 assays 230.58€ Pub
M1245 MarkerGeneTM Fluorescent Cellulase Assay Kit, Allows fast and easy detection of most cellulases in a microtiter plate based assay format, 1 kit MarkerGene 367.82€
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Pub
K331-100 HDAC Colorimetric Activity Assay Kit Biovision 100 assays 357.00€
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Pub
K752-100 Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit Biovision 100 assays 357.00€ Pub
22813 Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit * Green Fluorescence* AAT Bioquest 1 kit 215.00€
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Pub
BQ007-EAEL ENZYMATIC ASSAY KITS (CHEMISTRY AUTOANALYZER), Electrolytes: CO2 (405nm) Assay Kit (Enzymatic, liquid stable) Bioquant 300 tests 319.00€ Pub
K711-100 Amylase Activity Assay Kit Biovision 100 assays 269.00€ Pub
4700-192-K Tankyrase 1 Colorimetric Activity Assay Trevigen 192 samples 1 342.00€
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Pub
K335-100 SOD Activity Assay Kit 100 assays Biovision 100 assays 230.58€ Pub
M0795 MarkerGeneTM Live Dead Assay Kit, Allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity(live cells) and staining of nucleic acid in chromatin (dea MarkerGene 299.00€
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Pub
22822 Cell Meter™ Generic Fluorometric Caspase Activity Assay Kit *Optimized Red Fluorescence for Flow Cytometry* AAT Bioquest 1 kit 231.00€
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Pub
BQ062A-EACN ENZYMATIC ASSAY KITS (CHEMISTRY AUTOANALYZER), KIDNEY AND PANCREATIC DISEASES: NAG Control Set (2 x 2 mL vial) Bioquant 180.00€ Pub
K266-25 Nuclear Cytosol Fractionation Kit25 assays Biovision 25 assays 164.43€
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Pub
22900 Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence* AAT Bioquest 1 kit 202.00€
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Pub
P-3003-1 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K9 EpigenTek 48 assays 461.00€ Pub
K724-100 Lipase Activity Assay Kit III Biovision 100 assays 425.00€ Pub
22797 Cell Meter™ Caspase 3 7 Activity Apoptosis Assay Kit *Red Fluorescence* AAT Bioquest 100 Tests 280.00€
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Pub
K144-100 Cathepsin S Activity Assay Kit Biovision 100 assays 357.00€
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Pub
K779-100 DPP4 Activity Assay Kit Biovision 100 assays 386.00€
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Pub
K422-500 Alkaline Phosphatase Activity Fluorometric Assay Kit Biovision 500 assays 260.00€ Pub
K269-500 Mammalian Cell Extraction Kit500 assays Biovision 500 assays 221.13€ Pub
K330-100 HDAC Fluorometric Activity Assay Kit Biovision 100 assays 357.00€
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Pub
DACE-100 QuantiChrom™ Acetylcholinesterase Assay Kit, Quantitative determination of acetylcholinesterase activity by colorimetric (412nm) method. Procedure 10 min. Kit size 100 tests. Detection limit 10 U L Bioassays 100tests 385.00€ Pub
K745-100 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit Biovision 100 assays 406.00€ Pub
13512 Amplite™ MMP 3 Activity Assay Kit *Green Fluorescence* AAT Bioquest 1 kit 280.00€
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Pub
K757-100 Glucose-6-Phosphate Dehydrogenase Activity Assay Kit Biovision 100 assays 357.00€ Pub
22812 Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit * Blue Fluorescence* AAT Bioquest 1 kit 215.00€
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Pub
K787-100 Alcohol Dehydrogenase Activity Assay Kit Biovision 100 assays 386.00€ Pub
ECAT-100 EnzyChrom™ Catalase Assay Kit, Quantitative determination of catalase activity by colorimetric (570nm) or fluorometric (530nm 590nm) methods. Procedure 40 min. Kit size 100 tests. Detection limit 0 Bioassays 100tests 258.00€ Pub
K710-100 Xanthine Oxidase Activity Assay Kit Biovision 100 assays 289.00€ Pub
M1319 MarkerGeneTM Fluorescent Protease Assay Kit, Allows quick and simple measurement of general protease activity in biological samples. MarkerGene 261.00€
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Pub
4700-096-K Tankyrase 1 Colorimetric Activity Assay Trevigen 96 samples 996.00€ Pub
K335-100 SOD Activity Assay Kit Biovision 100 assays 279.00€ Pub
DTBA-100 QuantiChrom™ TBARS Assay Kit Bioassays 100 135.00€ Pub
ELH-CEA-001 Human CEA ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-p53-001 Custom Human p53 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 677.00€ Pub
ELM-Lipocalin2-001 Custom Mouse Lipocalin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€
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Pub
IMPAI-1mg Mouse PAI-1 (wild type active form) 1mg Innovative Research INC 1 571.00€ Pub
1069-200 Caspase 6 Substrate VEID AFC Biovision 200 assays 311.85€
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Pub
K360-100 Beta Secretase Fluorometric Assay Kit Biovision 100 assays 464.00€
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Pub
K189-25 Caspase 9 Staining Kit, CaspGLOW Green Biovision 25 assays 165.00€
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Pub
M0817 Green FAM Caspase 1 Assay Kit 25tests MarkerGene 270.00€
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Pub
Y058080 Androgen Receptor ABM Goods 100ul 148.05€
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Pub
20323T Mouse anti-bovine type II collagen IgG2b antibody subtype assay kit, TMB Chondrex 400.05€
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Pub
3457-096-K Cultrex 96 Well Collagen I Cell Invasion Assay Trevigen 96 samples 401.00€ Pub
DBGD-100 QuantiChrom™ â Glucosidase Assay Kit Bioassays 100 405.00€ Pub
ELF-RANTES-001 Custom Feline RANTES ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€
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ELH-IL8-001 Human IL-8 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-Eotaxin2-001 Mouse Eotaxin-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ENH3-100 EnzyChrom™ Ammonia Assay Kit Bioassays 100 274.00€ Pub
1002-200 Annexin V Cy3 Reagent Biovision 200 assays 315.50€
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Pub
K139-100 Caspase 12 Fluorometric Assay Kit Biovision 100 assays 360.50€
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Pub
K120-50 Quick Apoptotic DNA Ladder Detection Kit Biovision 50 assays 338.00€
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Pub
  P-4013-48  EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Fluorometric) (48 assays) EpigenTek 370.00€
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Pub
PEL-Met-Y1234-001 Human Mouse Rat Phospho-Met (Y1234) ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 435.00€ Pub
13802 Amplite™ Fluorimetric Alanine Aminotransferase Assay Kit AAT Bioquest 1 kit 280.00€
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Pub
EIA-CART-1 Human Mouse Rat CART EIA ELISA Ray Biotech One 96-Well Strip Microplate Kit 345.00€ Pub
ELH-HAI2-001 Custom Human HAI-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-TSLP-001 Human TSLP ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELP-IL6-001 Porcine IL-6 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
M35612759 5 Formyl 2 thiopheneboronic acid CAS Number [4347 33 5] Molekula 1 G 135.00€
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K114-100 Caspase 6 Fluorometric Assay Kit Biovision 100 assays 315.50€
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Pub
1110-1000 Caspase 4 Substrate LEVD pNA Biovision 1000 assays 634.10€
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Pub
K635-100 Creatine Assay Kit Biovision 100 assays 357.00€ Pub
K793-100 MMP-3 Inhibitor Screening Kit Biovision 100 assays 503.00€ Pub
BIO-37042 DNA kits, Column-free Isolation: SureClean Bioline 1 x 5ml 62.00€
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Pub
ZFN500 Zinc Formalin Solution Scy tek 500 ml 79.00€
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Pub
11304 Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 163.00€
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Pub
2052T Human monkey anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 518.81€
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Pub
60300 PDE Assay Kit BPS Bioscience 96 reactions 491.00€
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Pub
EAPL-200 EnzyChrom™ Adipolysis Assay Kit Bioassays 200 338.00€ Pub
ELH-DcR3-001 Custom Human DcR3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-PSAfree-001 Custom Human PSA-free ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-MPO-001 Mouse MPO ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498.00€ Pub
QT615-20 SensiMix SYBR & Fluorescein Kit (formally SensiMixPlus SYBR & Fluorescein) Bioline 2000 rxn 1 900.00€
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Pub
IRbPAI-0.5mg Rabbit PAI-1 (wild type active form 0.5mg Innovative Research INC 980.00€
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Pub
1072-1000 Caspase 2 Substrate VDVAD pNA1000 assays Biovision 1000 assays 579.29€
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Pub
K201-100 Annexin V FITC Apoptosis Kit Plus Biovision 100 assays 333.00€
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Pub
M0833 Red Sulforhodamine Poly Caspases Assay Kit 25tests MarkerGene 289.00€
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Pub
A166670 5α-N-Acetyl-2'H-androst-2-eno[3,2-c]pyrazol-17-one C21H27N2O2 CAS: TRC 25 mg 245.00€
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2-1501-001 Strep Tactin(R) Coated Microplate Kit ACR 1 plate 261.00€
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Pub
20334 Mouse anti-porcine type II collagen IgG3 antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
36309 Screen Quest™ Fluo 8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 100 Plates 1 571.00€
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Pub
DICU-250 QuantiChrom™ Copper Assay Kit Bioassays 250 430.00€ Pub
ELH-APRIL-001 Custom Human APRIL ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-MBL-001 Custom Human MBL ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-HAI1-001 Custom Mouse HAI-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ETGA-200 EnzyChrom™ Triglyceride Assay Kit Bioassays 200 352.00€ Pub
1008-1000 Caspase 3 Substrate DEVD pNA1000 assays Biovision 1000 assays 541.00€
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Pub
K151-100 Caspase 1 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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Pub
  P-4021-48  EpiQuik Global Acetyl Histone H3K56 Quantification Kit (Fluorometric) (48 assays) EpigenTek 370.00€
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Pub
POPN-500 pNPP Phosphatase Assay Kit Bioassays 500 187.00€ Pub
2-N801-R3 PET Roll; pore size( IT4IP quantity of 10m 1 123.00€
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Pub
22844 Cell Meter™ TUNEL Apoptosis Assay Kit AAT Bioquest 1 kit 280.00€
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Pub
CBEL-Stat-SK Human Mouse Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA (2) ELISA Ray Biotech Two 96-Well Microplate Kit 607.00€ Pub
EIA-PYY-1 Human Mouse Rat PYY EIA ELISA Ray Biotech One 96-Well Strip Microplate Kit 345.00€ Pub
ELH-IGG-001 Human IgG ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-BCMA-001 Custom Mouse BCMA ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELR-IL13-001C Rat IL-13 ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
M41628435 Indole 5 carboxaldehyde (5 Formylindole) CAS Number [1196 69 6] Molekula 5 G 259.00€
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K118-100 Caspase 9 Fluorometric Assay Kit Biovision 100 assays 342.50€
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Pub
1116-1000 Caspase-13 Substrate LEED-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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Pub
K671-100 Ascorbic Acid Assay Kit II (FRASC) Biovision 100 assays 406.00€ Pub
P-1014-96  Methylamp Global DNA Methylation Quantification Kit (96 assays) EpigenTek 808.50€
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Pub
11559 Amplite™ Luminometric Peroxidase Assay Kit *Luminescence* AAT Bioquest 1 kit 231.00€
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Pub
21665 PhosphoWorks™ Colorimetric Phosphate Assay Kit *Blue Color* AAT Bioquest 1 kit 202.00€
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Pub
80106 POP assay kit BPS Bioscience 100 reactions 550.00€
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Pub
ECLC-100 EnzyChrom™ Lactate Assay Kit Bioassays 100 371.00€ Pub
ELH-ErbB3-001 Human ErbB3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-Siglec5-001 Human Siglec-5 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-Pselectin-001 Mouse P-Selectin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
BUF058C PERMANENT AQUEOUS MOUNTING MEDIUM , Product Type Accessory Reagent, Specificity AQUEOUS MOUNTING MEDIUM, Target Species , Host N A, Format Ready To Use, Isotypes , Applications C, P, Clone AbD Serotec 250 ml 544.95€
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K015-F1 Acetylcholinesterase Fluorescent Activity kit (2 plate) Arbor Assays 2x96 well plates 293.00€
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Pub
11001 ReadiUse™ ABTS Solution *Optimized for ELISA Assays with HRP Conjugates AAT Bioquest 1 L 192.00€
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Pub
K510-100 Nickel Assay Kit Biovision 100 assays 367.00€ Pub
K261-100 Glutathione Colorimetric Detection Kit Biovision 100 assays 367.00€ Pub
M1193 MarkerGeneTM Gaussia Luciferase Cellular Assay Kit, Utilizes a new luciferase which does not require ATP and can be expressed in mammalian cells using commercially available reporter plasmids, 1 kit MarkerGene 140.00€
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Pub
A637360 5α-Androstan-3β-ol � C19H32O� CAS: 1224-92-6 TRC 100 mg 212.00€
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Pub
1012T Mouse anti-bovine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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Pub
20354T Mouse anti-human type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 442.05€
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Pub
36374 Screen Quest™ Fluorimetric ELISA cAMP Assay Kit AAT Bioquest 10 plates 1 571.00€
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Pub
DNH3-200 QuantiFluo™ Ammonia Assay Kit Bioassays 200 tests 109.00€ Pub
ELH-BNGF-001 Human beta-NGF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-MPIF1-001 Human MPIF-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-IL3-001 Mouse IL-3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€
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Pub
AS03037 RbcL | Rubisco large subunit, form I and form II (100 ug) Agrisera 100 ug 422.00€
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Pub
1062-1000 Caspase-8 Substrate IETD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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Pub
K303-2500 VisionBlue™ Quick Cell Viability Assay Biovision 2500 assays 564.00€
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Pub
K180-100 Caspase Staining Kit, CaspGLOW Green Biovision 100 assays 400.00€
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Pub
228-11413-1 Recombinant Human SCF KITLG [from Sf9] Proteins Ray Biotech 2 147.00€
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Pub
  P-4029-48  EpiQuik Global Acetyl Histone H4K12 Quantification Kit (Fluorometric) (48 assays) EpigenTek 373.00€
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Pub
SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.37€ Pub
20312T Mouse anti-chick type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 400.05€
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ELC-IFNgamma-001 Custom Canine IFN-gamma ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-IL1sRII-001 Custom Human IL-1 sRII ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-Clusterin-001 Custom Mouse Clusterin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELR-MCP1-001C Rat MCP-1 ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
  OP-0004-2  CytoX Red Cell Proliferation Cytotoxicity Assay Kit (5x96 assays) EpigenTek 214.00€
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K124-100 Caspase 10 Fluorometric Assay Kit Biovision 100 assays 360.50€
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Pub
4256-010-CC CometAssay Control Cells Trevigen 10 assays 177.00€ Pub
K723-100 Lipase Assay Kit II Biovision 100 assays 389.00€ Pub
P-1037-48 MethylFlash Hydroxymethylated DNA Quantification Kit Fluorometric EpigenTek 48 assays 507.82€
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PEL-Akt-S473-001 Human Mouse Rat Phospho-Akt (S473) ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 435.00€ Pub
130-10000-1000 Rabbit Anti-Human Androgen Receptor Ray Biotech 1000 766.00€ Pub
EFNP-100 EnzyFluo™ NADP NADPH Assay Kit Bioassays 100 tests 301.00€ Pub
ELH-FSH-001 Custom Human FSH ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-Thrombomodulin-001 Custom Human Thrombomodulin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-Testocam3-001 Custom Mouse Testocam 3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
19854 Cellufine Formyl , 500 ml Chisso 500 ml 3 400.95€
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K110-100 Caspase 1 Fluorometric Assay Kit Biovision 100 assays 315.50€
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Pub
1103-200 Caspase-1 Substrate YVAD-AFC; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K616-100 Glucose and Sucrose Assay Kit Biovision 100 assays 386.00€ Pub
K363-100 HDAC-3 Inhibitor Screening Kit Biovision 100 assays 503.00€
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Pub
M1405 MarkerGene™ Cellular Senescence Microtiterplate Assay Kit, This kit uses the high sensitivity substrate fluorescein di â D galactopyranoside (FDG) to quantify senescent cells, 1 kit MarkerGene 316.00€
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Pub
M13585136 19 Hydroxy 4 androstene 3,17 dione CAS Number [510 64 5] Molekula 25 MG 88.00€
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Pub
1031 Human monkey anti-chick type I collagen IgG antibody assay kit, OPD Chondrex 528.36€
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Pub
20365 Mouse anti-mouse type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 485.10€
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Pub
50031 HDAC assay buffer BPS Bioscience 20 ml 61.00€
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Pub
ELH-GASP1-001 Custom Human GASP-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-TNFbeta-001 Human TNF-beta ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-TPO-001 Mouse TPO ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
676944335 Cellufine Formyl Media AGTC Bioproducts Ltd 10 lt 0.00€
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K111-200 Caspase 1 Colorimetric Assay Kit Biovision 200 assays 450.50€
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Pub
1104-1000 Caspase 1 Substrate YVAD pNA1000 assays Biovision 1000 assays 541.49€
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Pub
K621-100 Galactose Assay Kit Biovision 100 assays 357.00€ Pub
K612-100 Free Fatty Acid Quantification Kit Biovision 100 assays 425.00€ Pub
M1580 MarkerGene™ Multiple Drug Resistance Microtiterplate Assay Kit, High-throughput assay system based on measurement of efflux of green fluorescent dye known to bind to MDR transporters ABCG2 and ABCB1 MarkerGene 310.00€
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Pub
YIAE1018AG.1 Uroguanylin (circulating form) aa 1 24, Human synthetic peptide Accurate 10 742.00€
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Pub
1033T Human monkey anti-porcine type I collagen IgG antibody assay kit, TMB Chondrex 528.36€
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Pub
2042 Rat anti-bovine type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
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Pub
52001 Methyltransferase G9a Assay Kit BPS Bioscience 100 reactions 530.00€
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Pub
E2HL-100 EnzyChrom™ AF HDL and LDL VLDL Assay Kit Bioassays 100 425.00€ Pub
ELH-CNTF-001 Human CNTF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-PDGFAA-001 Human PDGF-AA ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-MBL2-001 Custom Mouse MBL-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€
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Pub
IMTPAKT Active Mouse tPA Functional Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
1070-200 Caspase-6 Substrate VEID-pNA; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K375-100 Urea Assay Kit Biovision 100 assays 377.00€ Pub
K190-25 CaspGLOW Red Active Caspase Staining Kit Biovision 25 assays 200.00€
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Pub
M0822 Green FAM Caspases 3 and 7 Assay Kit 100tests MarkerGene 651.00€
ASK
Pub
Y102929 Androgen Receptor Ab 1 ABM Goods 200ug 1 589.70€
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Pub
2032T Mouse anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 387.45€
ASK
Pub
ELH-Adipsin-001 Human Adipsin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-LAG3-001 Custom Human LAG3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-FetuinA-001 Custom Mouse Fetuin A ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
EPHE-100 EnzyChrom™ Phenylalanine Assay Kit Bioassays 100 tests 253.00€ Pub
1004-1000 Annexin V EGFP Reagent1000 assays Biovision 1000 assays 891.00€
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Pub
K129-25 Annexin V PE Cy5 Apoptosis Detection Kit Biovision 25 assays 207.50€
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Pub
  P-4015-96  EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Fluorometric) (96 assays) EpigenTek 618.00€
ASK
Pub
PEL-Stat1-S727-002 Human Mouse Phospho-Stat 1 (S727) and Total Stat 1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 470.00€ Pub
15259 Amplite™ Fluorimetric NADP NADPH Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
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Pub
22826 Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry* AAT Bioquest 1 kit 202.00€
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Pub
BS-060102-AAI INTELIWASHER Microplate washer Biosan each 3 264.82€ Pub
EIA-GIP-1 Human Mouse Rat GIP EIA ELISA Ray Biotech One 96-Well Strip Microplate Kit 345.00€ Pub
ELH-HGF-001C Human HGF ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-VEGFD-001 Custom Human VEGF-D ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELR-bNGF-001 Rat beta-NGF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
A609650 4-Amino-5-formylamino-3-isobutyl-1-methylpyrimidine-2,6-dione C10H16N4O3 CAS: 130332-59-1 TRC 1 g 193.00€
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Pub
K115-200 Caspase 6 Colorimetric Assay Kit Biovision 200 assays 450.50€
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Pub
1113-1000 Caspase-10 Substrate AEVD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
ASK
Pub
K646-100 Glycogen Assay Kit 100 assays Biovision 100 assays 364.51€ Pub
K812-1000 BCA Protein Quantitation Kit Biovision 1000 assays 240.00€ Pub
MPR-406 ELISA Microplate Reader (compact, 8 channel, automated, programmable) with 6 filters & ELISA reader software Species Reactivity: ADI 1 Product tipe: Instrument 6 290.00€
ASK
Pub
11402 Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
2061 Human monkey anti-chick type II collagen IgA antibody assay kit, OPD Chondrex 518.81€
ASK
Pub
60400 PDE10A Assay Kit BPS Bioscience 96 reactions 491.00€
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Pub
ELH-ECadherin-001 Custom Human E-Cadherin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-Resistin-001 Human Resistin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-OPN-001 Mouse OPN ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
M17425989 Formamide Molecular biology grade, deionized CAS Number [75 12 7] Molekula 2x5L 197.00€
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Pub
IRbTPAKT Active Rabbit tPA Functional Assay ELISA Kit Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
1075-1000 Caspase 9 Substrate LEHD AFC Biovision 1000 assays 613.31€
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Pub
K421-500 Acid Phosphatase Fluorometric Assay Kit Biovision 500 assays 250.00€ Pub
K202-400 Annexin V Cy3 Apoptosis Kit Plus Biovision 400 assays 666.50€
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Pub
M0838 Magic RedTM (DEVD)2 Caspases 3 and 7 Assay Kit 100tests MarkerGene 560.00€
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Pub
A637565 Androst-4-ene-3,6,17-trione C19H24O3 CAS: 2243-06-3 TRC 10 mg 212.00€
ASK
Pub
HPS999 H. Pylori Rapid Stain (1-Step) Scy tek 1000 ml 117.00€
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Pub
2035 Mouse anti-human type II collagen IgG antibody assay kit, OPD Chondrex 434.70€
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Pub
36336 Cal-520™ Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 10 plates 436.00€
ASK
Pub
DIGT-250 QuantiChrom™ Glutathione Assay Kit Bioassays 250 341.00€ Pub
ELH-BCAM-001 Human BCAM ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-MICA-001 Human MICA ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-IFNgamma-001C Mouse IFN-gamma ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
CSB-E15075r Rat Forkhead Box P3(FOXP3) ELISA Kit, Species Rat, Sample Type serum, plasma Cusabio 96T 913.50€
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Pub
1013-1000 Annexin V Cy5 Reagent1000 assays Biovision 1000 assays 937.00€
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Pub
K156-100 Caspase 6 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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Pub
  P-4023-96  EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Fluorometric) (96 assays) EpigenTek 606.00€
ASK
Pub
QFPR-200 QuantiFluo™ Protein Assay Kit Bioassays 200 tests 109.00€ Pub
20113 Cell Meter™ Live Cell Caspase 6 Binding Assay Kit *Green Fluorescence* AAT Bioquest 25 Tests 280.00€
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Pub
24054 Primary antibody CIDE-A Antibody, Source Rabbit, Species Reactivity It has no cross activity to CIDE-B. SignalWay Antibody S.A.B 100ug 364.00€
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Pub
CBEL-STAT6-1 Human Mouse Phospho-Stat 6 (Y641) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347.00€ Pub
EIA-ZAG-1 Human Mouse Rat ZAG EIA ELISA Ray Biotech One 96-Well Strip Microplate Kit 345.00€ Pub
ELH-IL17BR-001 Custom Human IL-17B R ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-CCC5a-001 Custom Mouse C5a ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELR-IL2-001 Rat IL-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
M67618434 2 Fluoro 5 formylphenylboronic acid CAS Number [352534 79 3] Molekula 1 G 352.00€
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K119-200 Caspase 9 Colorimetric Assay Kit Biovision 200 assays 468.50€
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1116-200 Caspase 13 Substrate LEED AFC200 assays Biovision 200 assays 276.89€
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K689-100 PicoProbe™ Fructose-6-Phosphate Assay Kit Biovision 100 assays 425.00€ Pub
P-1020-48 FitAmp General DNA Quantification Kit EpigenTek 48 assays 160.65€
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P-3014T-096 EpiQuik In Situ Histone H3K27 Tri Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
11956 Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence* AAT Bioquest 1 kit 202.00€
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22784 Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence with 405 nm Excitation* AAT Bioquest 1 kit 202.00€
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80209 DPP9 assay kit BPS Bioscience 100 reactions 557.00€
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ECTX-100 EnzyLight™ Cytotoxicity Assay Kit Bioassays 100 220.00€ Pub
ELH-Fas-001C Human Fas ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-STNFRII-001 Human sTNFRII ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-SCF-001 Mouse SCF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
SCH-MCA4610T MOUSE ANTI HUMAN CD15, Product Type Monoclonal Antibody, Specificity CD15, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applications C, F, P, Clone HI98 Serotech 25 µg 148.05€
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K033-F1 Catalase Fluorescent Activity kit (Two Plates) Arbor Assays 2 x 96 well plate 310.00€ Pub
1101-200 Caspase 5 Substrate WEHD AFC Biovision 200 assays 311.85€
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K566-100 Sialic Acid (NANA) Assay Kit Biovision 100 assays 435.00€ Pub
K267-50 Cytosol/Particulate Separation Kit Biovision 50 assays 322.25€ Pub
M1227 8 Octadecyloxypyrene 1,3,6 trisulfonic acid trisodium salt, Highly water soluble polar fluorescent lipid probe for the sensitive fluorimetric assay of cationic surfactants, 10mg MarkerGene 223.00€
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EIAAB27789 CAR,Car,Constitutive androstane receptor,Nr1i3,Nuclear receptor subfamily 1 group I member 3,Rat,Rattus norvegicus EIAab 1 191.00€
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1016T Mouse anti-mouse type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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20361 Mouse anti-mouse type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 485.10€ Pub
36401 Cal-520™ PBX Calcium Assay Kit *Optimized for Probenecid-Sensitive Cell Lines* AAT Bioquest 100 plates 3 911.00€
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DNM-9602G Microplate Reader Perlong 1 2 883.00€ Pub
ELH-CathepsinL-001 Custom Human Cathepsin L ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-Nidogen1-001 Human Nidogen-1 Ray Biotech One 96-Well Strip Microplate Kit 498.00€ Pub
ELM-IL6-001 Mouse IL-6 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€
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IHATIIIKT-TOT Human Antithrombin III total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
1062-200 Caspase 8 Substrate IETD AFC200 assays Biovision 200 assays 276.89€
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K317-100 PicoProbe™Acetyl CoA Assay Kit Biovision 100 assays 351.50€ Pub
K182-25 CaspGLOW Fluorescein Active Caspase 2 Staining Kit Biovision 25 assays 200.00€
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LC2081 Multiple lung carcinoma (Non small cell lung carcinoma, NSCLC) and normal tissue form necroscopy tissue array, 70 cases 208 cores, with stage and grade information US Biomax 456.00€
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  P-4032-96  EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) (96 assays) EpigenTek 606.00€
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STA-330 OxiSelect™ TBARS Assay Kit (MDA Quantitation) Cell Biolabs 200 assays 489.00€ Pub
20314T Mouse anti-chick type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 402.15€
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30029 NUC VIEW 488 CASPASE 3 ASSAY KIT Biotium 1 KIT 496.65€ Pub
DACE-100 QuantiChrom™ Acetylcholinesterase Assay Kit Bioassays 100 385.00€ Pub
ELC-SCF-001 Custom Canine SCF Ligand ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-IL2Ralpha-001 Custom Human IL-2 R alpha Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-CXCL16-001 Mouse CXCL16 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELR-TCK1-001 Rat TCK-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498.00€ Pub
  P-4004-192  EpiQuik In Situ H3K9 Acetylation Assay Kit (2x96 assays) EpigenTek 841.00€
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K125-200 Caspase 10 Colorimetric Assay Kit Biovision 200 assays 495.50€ Pub
K101-100 Annexin V FITC Apoptosis Kit Biovision 100 assays 333.00€ Pub
P-1040-96 MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (fluorometric) (96 assays) EpigenTek 649.00€ Pub
PEL-EGFR-Y1045-002 Human Phospho-EGFR (Y1045) and Total EGFR ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 470.00€ Pub
22801 Cell Meter™ JC 10 Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays* AAT Bioquest 1 kit 280.00€
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AAM-ISO-G1-2 Rapid Mouse Ig Isotyping Array 1 (2) Antibody Arrays Isotyping Arrays Ray Biotech 16-22 Sample Kit 430.00€ Pub
EGLT-100 EnzyChrom™ Glutamate Assay Kit Bioassays 100 360.00€ Pub
ELH-IL5-001 Human IL-5 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-Dtk-001 Custom Mouse Dtk ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELR-VEGF-001C Rat VEGF-A ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
1001-1000 Annexin V FITC Reagent Biovision 1000 assays 945.50€
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K126-400 Caspase 4 Fluorometric Assay Kit Biovision 400 assays 738.50€ Pub
K104-100 Annexin V EGFP Apoptosis Kit Biovision 100 assays 333.00€
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  P-4010-48  EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
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PEL-EGFR-Y992-001 Human Phospho-EGFR (Y992) ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 435.00€ Pub
13620 Amplite™ Colorimetric Sphingomyelinase Assay Kit AAT Bioquest 1 kit 212.00€
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22811 Cell Meter™ Nuclear Apoptosis Assay Kit AAT Bioquest 1 kit 231.00€
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AQT1352 Human Glucocorticoid Receptor Activity Test Orgenium 96 Tests 1 050.00€
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EIA-ACRP-1 Human Mouse Rat ACRP30 EIA ELISA Ray Biotech One 96-Well Strip Microplate Kit 345.00€ Pub
ELH-GH-001 Human GH ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-TRAILR2-001 Human TRAIL R2 Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-TSLP-001 Mouse TSLP ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
A597550 6-Amino-1-benzyl-5-(N-formyl-N-methyl)uracil C13H14N4O3 CAS: 72816-89-8 TRC 250 mg 232.00€
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K112-25 Caspase 8 Fluorometric Assay Kit Biovision 25 assays 162.50€
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1109-1000 Caspase 4 Substrate LEVD AFC Biovision 1000 assays 613.31€
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K628-100 Maltose Assay Kit Biovision 100 assays 357.00€ Pub
K639-100 L Amino Acid Quantitation Kit Biovision 100 assays 333.50€ Pub
MD-14-0252P Recombinant Human SCF KITLG Proteins Ray Biotech 10 309.00€
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SL.FSOLV.05 5 L F-Solv 100% Formaldehyde free Buffered pH Neutral (Replaces the known fixator formaldehyde 40%) Yvsolab 5L 75.00€
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1043 Human monkey anti-porcine type I collagen IgA antibody assay kit, OPD Chondrex 528.36€
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2044T Rat anti-rat type II collagen IgG antibody assay kit, TMB Chondrex 434.70€
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52008 Methyltransferase MLL Assay Kit BPS Bioscience 100 reactions 530.00€
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EACL-100 EnzyChrom™ Acetylcholine Assay Kit Bioassays 100 371.00€ Pub
ELH-CRTAM-001 Custom Human CRTAM ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-PDGFRbeta-001 Human PDGF R beta ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-MIG-001 Mouse MIG ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€
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IPOPAI-0.5mg Porcine PAI-1 (wild type active form) 0.5mg Innovative Research INC 928.00€
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1071-1000 Caspase 2 Substrate VDVAD AFC1000 assays Biovision 1000 assays 541.49€
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K390-100 Iron Assay Kit Biovision 100 assays 377.00€ Pub
K198-100 CaspGLOW Red Active Caspase 8 Staining Kit Biovision 100 assays 482.00€
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M0827 Green FAM Caspase 9 Assay Kit 25tests MarkerGene 270.00€
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Y213377 Androgen Receptor ABM Goods 200ul 520.80€
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20332 Mouse anti-porcine type II collagen IgG2a antibody subtype assay kit, OPD Chondrex 400.05€
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36005 Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence* AAT Bioquest 10 plates 436.00€
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DIAP-250 QuantiChrom™ BCP Albumin Assay Kit, Quantitative determination of albumin by bromcresol purple BCP method at 610nm. Procedure 5 min. Kit size 250 tests. Detection limit 0.3 g dL. Shelf life 12 mon Bioassays 250tests 299.00€ Pub
ELH-ANG-001 Human ANG ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-LIMPII-001 Human LIMP-II ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498.00€ Pub
ELM-GAS1-001 Custom Mouse GAS1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
EPYR-100 EnzyChrom™ Pyruvate Assay Kit Bioassays 100 338.00€ Pub
1007-1000 Caspase 3 Substrate DEVD AFC1000 assays Biovision 1000 assays 541.00€
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K252-200 Nitric Oxide Fluorometric Assay Kit Biovision 2 x 96 assays 431.00€
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K133-9-25 Caspase Fluorometric Substrate Set II Biovision 9 x 25 assays 687.00€
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  P-4018-48  EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
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PLDS-ADPL Peptoid Ligand Assay Development Drug Discovery Service Peptoid Ligand Screening Ray Biotech 0.00€
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15264 Amplite™ Fluorimetric NADP NADPH Ratio Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 319.00€
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22831 Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Optimized Green Fluorescence for Flow Cytometry* AAT Bioquest 1 kit 231.00€
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CBEL-EGFR1068-1 Human Phospho-EGFR (Y1068) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347.00€ Pub
EIA-NEB-1 Human Mouse Rat Neurokinin-B EIA ELISA Ray Biotech One 96-Well Strip Microplate Kit 345.00€ Pub
ELH-HVEM-001 Human HVEM ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498.00€ Pub
ELM-6Ckine-001 Mouse 6Ckine ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELR-EGF-001 Custom Rat EGF ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
M37091733 4 Formylimidazole (4(5) Imidazolecarboxaldehyde) CAS Number [3034 50 2] Molekula 1 G 88.00€
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K116-25 Caspase 2 Fluorometric Assay Kit Biovision 25 assays 171.50€
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1114-1000 Caspase-10 Substrate AEVD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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K656-100 Isocitrate Assay Kit Biovision 100 assays 357.00€ Pub
OP-0002-1  EpiQuik Nuclear Extraction Kit (100 assays) EpigenTek 239.40€ Pub
NWK-TAC01 NWLSS™ TAC Peroxyl Assay (Total Antioxidant Capacity) Northwest Life Science 96 678.68€ Pub
11540 Amplite™ Fluorimetric Goat Anti Mouse IgG HRP Conjugate ELISA Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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21609 PhosphoWorks™ Luminometric ATP Assay Kit *Steady Glow* AAT Bioquest 1 kit 124.00€
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7500-100-K SOD Assay Kit Trevigen 100 reactions 518.00€
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ECAT-100 EnzyChrom™ Catalase Assay Kit Bioassays 100 274.00€ Pub
ELH-Endoglin-001 Human Endoglin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-SDF1beta-001 Custom Human SDF-1 beta ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELM-PIGF2-001 Mouse PLGF-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
0100-0483 MOUSE ANTI BOVINE ROTAVIRUS MR STRAIN, Product Type Monoclonal Antibody, Specificity ROTAVIRUS MR STRAIN, Target Species Viral, Host Mouse, Format Purified, Isotypes IgG2a, Applications E, Clon AbD Serotec 0.2 mg 338.00€
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IRPAIKT-TOT Rat PAI-1 total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 575.00€ Pub
1076-1000 Caspase-9 Substrate LEHD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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K470-100 Ammonia Assay Kit II Biovision 100 assays 386.00€ Pub
K250-25 MitoCapture Apoptosis Detection Kit Biovision 25 assays 213.00€
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M0844 Magic RedTM (FR)2 Cathepsin L Assay Kit 100tests MarkerGene 560.00€
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A637555 (3β)-Androsta-5,16-diene-3,17-diol 3-Acetate 17-(Trifluoromethanesulfonate) Discontinued C22H29F3O5S CAS: 115375-60-5 TRC 25 mg 212.00€
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10055 Amplite™ Fluorimetric Glutathione Assay Kit *Green Fluorescence* AAT Bioquest 1 kit 163.00€
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20352 Mouse anti human type II collagen IgG2a assay kit Chondrex 550.20€
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36341 Screen Quest™ Fluorimetric MDR Assay Kit *10 Plates* AAT Bioquest 1 kit 791.00€
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DIPI-500 QuantiChrom™ Phosphate Assay Kit Bioassays 500 245.00€ Pub
ELH-bFGF-001 Human bFGF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELH-MIP1beta-001 Human MIP-1 beta ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-IL1alpha-001 Mouse IL-1 alpha ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€
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4861-25 Isopeptidase T (short form), human recombinant Biovision 25 μg 252.00€ Pub
1014-200 Annexin V PE Reagent Biovision 200 assays 315.50€
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K163-100 CaspSELECT Caspase 3 Immunoassay Kit Biovision 100 assays 649.00€
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228-11411-1 Recombinant Rat SCF KITLG Proteins Ray Biotech 2 147.00€ Pub
  P-4026-48  EpiQuik Global Acetyl Histone H4K16 Quantification Kit (Colorimetric) (48 assays) EpigenTek 358.00€
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RD272091100 Mouse, Adiponectin (Trimeric form) , Rec. Prot. Biovendor 0.1 mg 428.00€ Pub
2031 Mouse anti-chick type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
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290-63701 Triglyceride Assay Kit Lipids in serum consist of triglycerides, cholesterols, phospholipids, free fatty acids and slight amounts of fat soluble components such as fatsoluble vitamins and carotenes. T B-Bridge 1,000 tests 467.25€
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ELAC-100 EnzyChrom™ Lactose Assay Kit Bioassays 100 360.00€ Pub
ELH-IL1alpha-001 Human IL-1 alpha ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-CD32a-001 Custom Mouse CD32a ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELR-Jagged1-001 Custom Rat Jagged 1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
BRF404 Breast invasive ductal carcinoma forzen tissue array, 20 cases 40 cores US Biomax 167.00€
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K122-25 Caspase 5 Fluorometric Assay Kit Biovision 25 assays 171.50€
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1117-200 Caspase 12 Substrate ATAD AFC Biovision 200 assays 415.80€
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P-1034-48  MethylFlash Methylated DNA Quantification Kit (Colorimetric) (48 assays)  EpigenTek 458.85€ Pub
12532 Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* AAT Bioquest 100 plates 3 131.00€
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22790 Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence* AAT Bioquest 1 kit 220.00€
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80556 PARP6 Assay kit BPS Bioscience 32 reactions 892.00€
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EERK-100 EnzyFluo™ ERK Phosphorylation Assay Kit Bioassays 100 tests 381.00€ Pub
ELH-FetuinB-001 Custom Human Fetuin B ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
ELH-TECK-001 Human TECK ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362.00€ Pub
ELM-sTNFRII-001 Custom Mouse sTNFRII ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542.00€ Pub
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