Product name :CellQuanti MTT™ Cell Viability Assay Kits

Catalog Number :CQMT-01K

Quantity :1000

Price :230.45 €

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Supplier :Bioassays

DESCRIPTION
The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis).


The CellQuanti-MTTTM assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells.


The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.

 

KEY FEATURES
Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
Sensitive and accurate. As low as 950 cells can be accurately quantified.
Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
Robust and amenable to HTS. Z’ factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems.

 

APPLICATIONS
Cell Proliferation: effects of cytokines, growth factor, nutrients.
Cytotoxicity and Apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.
Drug Discovery: high-throughput screen for toxic and anticancer drugs.

 

KIT CONTENTS
Catalog #             Size (assays)      Reagent      Assay Buffer              Solubilizer
CQMT-500           500                       solid                   10 mL                     50 mL
CQMT-01K        1,000                       solid                   20 mL                   100 mL
CQMT-HTS      >5,000                      solid              customized                 customized

Control Reagent (Cat # CTTX-050): 50 mg saponin (to be ordered separately).

Storage conditions. Store the Reagent at -20 °C. Keep Assay Buffer andSolubilization Solution at 4 °C and room temperature, respectively.

Shelf life: 12 month after receipt.

Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.

 

PROCEDURES
The CellQuanti-MTTTM assay is based on the conversion of MTT to purple formazan by metabolically active cells. For most cells, this reducing reaction takes 3 to 5 hours. The produced formazan is  solubilized and the resulting colored solution is quantified with a microplate reader. Although most culture media contain phenol red, phenol red does not interfere with the assay. All data in Technical Notes were obtained in culture media containing phenol red.

Procedure using 96-well plate:
1. Plate and culture cells (80 μL per well) in a clear bottom 96-well tissue culture plates. Typical culture medium contains DMEM, 10% fetal bovine serum, antibiotics (penicillin/streptomycin, gentamycin, etc), amino acids and other nutrients. Assays can be performed on either adherent cells or cells in suspension. The number of cells can vary from 1,000 to 80,000 per well. The volume can vary from 50 to 150 μL, although 80 μL is used in this example. In addition to the test samples, one must include control wells of culture medium containing no cells or cells treated with a toxic reagent such as 0.1% saponin.
2. Add test compounds and controls and incubate cells for the desired period of time (typically overnight). It is recommended that assays be run in duplicate or triplicate. A volume of 20 μL in phosphate buffered saline (PBS) or culture medium is recommended for the test compounds and controls. The Control reagent can be conveniently reconstituted with 5 mL PBS (1% saponin).
3. Reconstitute the CellQuanti-MTTTM Reagent with Assay Buffer. First equilibrate the Reagent and Assay Buffer to room temperature. Then simply combine Assay Buffer and the Reagent by pipetting a small volume (e.g. 1 mL) buffer to the Reagent tube. Vortex briefly and pipet the reconstituted solution to the Assay Buffer bottle. Repeat this step to transfer all Reagent to the Assay Buffer bottle. Mix by inversion until the Reagent is thoroughly dissolved. After this is done, mark the bottle label as Reconstituted Reagent. Note: Fresh reconstitution is recommended although the reconstituted reagent is stable for up to 4 weeks when stored at -20 °C.
4. Add 15 μL (per 80 μL cell culture) of CellQuanti-MTTTM reagent per well and incubate for 4 hours at 37°C. The volume of the reagent should be adjusted depending on the volume of cell culture.
5. Add 100 μL of the Solubilization Solution. Mix gently on an orbital shaker for one hour at room temperature. The volume of the Solubilization Solution should be adjusted depending on the volume of
cell culture. If precipitation occurs in the Solubilization Solution, place the bottle in a warm water bath or at 37°C and shake to dissolve precipitates.
6. Measure OD570nm for each well on an absorbance plate reader. Maximum absorbance of the formazan dye lies between 560 and 590 nm. If desired, the OD measurement can be performed the following day. In this case, it is recommended to seal the plate to minimize evaporation.

 

DATA ANALYSIS
Determine the average of the blank controls and subtract this amount from all absorbance values. Plot the corrected absorbance values at 570nm against the concentration of the test compound. Determine the EC50 value for cell proliferation and IC50 value for cytotoxic compound by non-linear regression analysis using Prism or any other data analysis tools.

 

 

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228-10806-2 Recombinant Human T-cell Leukemia Virus 1 & 2 (HTLV-1 + HTLV-2) Ray Biotech 25 290.00€ Pub
60503 RARalpha Reporter Cell Line BPS Bioscience ~2x10(6) cells 8 240.00€
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Pub
DICL-250 QuantiChrom™ Chloride Assay Kit Bioassays 250 265.00€ Pub
IRbPAIKT Active Rabbit PAI-1 Functional Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K422-500 Alkaline Phosphatase Activity Fluorometric Assay Kit Biovision 500 assays 260.00€ Pub
M0817 Green FAM Caspase 1 Assay Kit 25tests MarkerGene 270.00€
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Y108219 BAFF (B cell activating factor) ABM Goods 50ug 639.45€
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DS-MB-03294 Mouse Anti-Human Follicular Dendritic Cells Antibodies Ray Biotech 25 155.00€
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19875 Cellufine Chelate , 50 ml Chisso 50 ml 362.25€
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675982327 Cellufine Q 500 , 100 ml Chisso 100 ml 234.15€ Pub
1075-200 Caspase-9 Substrate LEHD-AFC; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K250-25 MitoCapture Apoptosis Detection Kit Biovision 25 assays 213.00€
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MSP20 Rapid Microplate Assay Kit for PDH Activity and Quantity (Combo) ACR 96 Tests 988.05€ Pub
13802 Amplite™ Fluorimetric Alanine Aminotransferase Assay Kit AAT Bioquest 1 kit 280.00€
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22502 Cell Explorer™ Fixed Cell Labeling Kit *Orange Fluorescence with 405 nm Excitation* AAT Bioquest 1 kit 172.00€
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Pub
ES723 Esophageal squamous cell carcinoma tissue array, with stage, grade and normal tissue, 23 cases 72 cores, replacing BS02031 US Biomax 231.00€
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K867-10 DiscoveryPak™ Stem Cell Fate Regulator Set VII Biovision 10 Modulators 874.00€ Pub
QUANTIA-CRPUS Immunoturbidimetric assay for Ultra Sensitive C Reactive Protein Tulip Group 50 Test 43.90€ Pub
cAP-0007GFP GFP Expressing Human Coronary Artery Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
19845-15 MC Cellufine Sulfate , 1x5 ml Chisso 1x5 ml 213.15€ Pub
20600 Cellufine MAX Q h , 100 ml Chisso 100 ml 362.25€ Pub
1013-200 Annexin V Cy5 Reagent200 assays Biovision 200 assays 325.08€
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K163-100 CaspSELECT Caspase 3 Immunoassay Kit Biovision 100 assays 649.00€
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  P-4025-48  EpiQuik Global Acetyl Histone H4K8 Quantification Kit (Fluorometric) (48 assays) EpigenTek 358.00€
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Pub
11304 Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 163.00€
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Pub
20361 Mouse anti-mouse type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 485.10€ Pub
3003 MMP-13 inhibitor assay kit Chondrex 525.00€
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Pub
EDLC-100 EnzyChrom™ D-Lactate Assay Kit Bioassays 100 tests 285.00€ Pub
K122-100 Caspase 5 Fluorometric Assay Kit Biovision 100 assays 342.00€
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Pub
K705-400 PhosphoSeek™ PDE5A Assay Kit Biovision 400 Assays 669.00€ Pub
MD-14-1036 Rabbit Anti-Insect Cell Line SF9 Antibodies Ray Biotech 1 mL 510.00€
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228-11623-2 Recombinant Human VEGF VEGFA [from CHO cells] Proteins Ray Biotech 10 205.00€
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mAP-0138 Rat monoclonal anti mouse Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) Anti-Mouse antibodies AngoiPro 100.00 ug 310.00€ Pub
bs-2222R-Cy5.5 Rabbit Anti-cellulase Polyclonal Antibody, Cy5.5 Conjugated Bioss 100ug 369.00€
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1117-1000 Caspase 12 Substrate ATAD AFC Biovision 1000 assays 1 039.50€
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P-1020-96 FitAmp General DNA Quantification Kit EpigenTek 96 assays 239.40€
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1025 Rat anti-human type I collagen IgG antibody assay kit, OPD Chondrex 425.25€
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Pub
20314T Mouse anti-chick type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 402.15€
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80203 DPP3 assay kit BPS Bioscience 100 reactions 557.00€
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DIZN-250 QuantiChrom™ Zinc Assay Kit Bioassays 250 422.00€ Pub
K105-100 Caspase 3 Fluorometric Assay Kit Biovision 100 assays 315.50€ Pub
K572-100 Phenylalanine Assay Kit Biovision 100 assays 367.00€ Pub
M0832 Green FAM Caspase 13 Assay Kit 100tests MarkerGene 651.00€
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Pub
IC05-3054-25ml Mouse Red Blood Cells 25ml Innovative Research INC 25ml 248.00€ Pub
19905 Cellufine Butyl Media AGTC Bioproducts Ltd 100 ml 288.00€
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Pub
676951324 Cellufine Chelate Media AGTC Bioproducts Ltd 10 ml 192.00€ Pub
1102-1000 Caspase-5 Substrate WEHD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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Pub
K267-50 Cytosol Particulate Separation Kit Biovision 50 assays 308.00€ Pub
A00081 CD23, B-Cell; Clone MHM6 Scy tek 6 ml 249.00€
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18-783-75653 RABBIT ANTI HUMAN SDF-1 ALPHA - CXCL12a; SDF-1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; PBSF; hIRH Polyclonal GenWay 0.1 mg 625.00€
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22624 Cell Explorer™ Cell Tracking Kit *Deep Red Fluorescence* AAT Bioquest 200 Tests 202.00€
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50031 HDAC assay buffer BPS Bioscience 20 ml 61.00€
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CSB-EL005599RA Rat Mast Cell Chymase ELISA Kit, Species Rat, Sample Type serum, plasma Cusabio 96T 808.50€
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Pub
IHATIIIKT-TOT Human Antithrombin III total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K330-100 HDAC Fluorometric Activity Assay Kit Biovision 100 assays 357.00€
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Pub
LC242 Non small cell lung carcinoma (NSCLC) test tissue array, with unmatched normal adjacent tissue, 12 cases 24 cores US Biomax 88.00€
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Pub
cAP-0019 Human Saphenous Vein Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
19907-51 MC Cellufine Q-500 Media AGTC Bioproducts Ltd 5 x 1 ml 120.00€
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Pub
200-51 MC Cellufine ETclean L , 5x1 ml Chisso 5x1 ml 265.65€ Pub
1063-1000 Caspase 8 Substrate IETD pNA Biovision 1000 assays 613.31€
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Pub
K188-100 Caspase 8 Staining Kit, CaspGLOW Green Biovision 100 assays 400.00€
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Pub
11559 Amplite™ Luminometric Peroxidase Assay Kit *Luminescence* AAT Bioquest 1 kit 231.00€
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2042 Rat anti-bovine type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
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bs-7605R-PE-Cy3 Rabbit Anti-Cell death inducing protein C16orf5 Polyclonal Antibody, PE-Cy3 Conjugated , 22kDa; Isotype IgG; Reactivity Human , Mouse; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
EGLY-200 EnzyChrom™ Glycerol Assay Kit Bioassays 200 338.00€ Pub
K126-200 Caspase 4 Fluorometric Assay Kit Biovision 100 assays 495.50€
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K745-100 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit Biovision 100 assays 406.00€ Pub
P-2004-96 EpiQuik General Protein DNA Binding Assay Kit Colorimetric EpigenTek 96 assays 521.00€ Pub
5000-001-01 Rat Mesenchymal Cells Trevigen 1 vial 630.00€ Pub
Y090748 Anti bodywall muscle cells Monoclonal ABM Goods 100ul 300.00€
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  P-4008-96  EpiQuik Global Histone H3 Acetylation Assay Kit (96 assays) EpigenTek 709.00€
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Pub
K104-25 Annexin V EGFP Apoptosis Kit Biovision 25 assays 162.50€
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Pub
SMART-SPT6EM Mouse Sera SPOT Assays, Expanded Antigen Panel:CPIL, Ectromelia, LCM, Hantaan, Mouse Adenovirus FL K87, MCMV BTP 349.00€
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1051 TCCI T cell proliferation grade chick type I collagen, 0.5 mg Chondrex 153.30€
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Pub
2032T Mouse anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 387.45€
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Pub
E2ND-100 EnzyChrom™ NAD NADH Assay Kit Bioassays 100 tests 301.00€ Pub
K112-100 Caspase 8 Fluorometric Assay Kit Biovision 100 assays 315.50€
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Pub
K624-100 Lactose Assay Kit Biovision 100 assays 357.00€ Pub
FMS-1 Fontana-Masson Stain Kit (For Argentaffin Cells and Melanin) Scy tek 1 kit(s) 187.00€
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Pub
IC10-0410-100ml Bovine Red Blood Cells, Packed 10% 100ml Innovative Research INC 100ml 257.00€
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Pub
20400-51 MC Cellufine MAX S-h Media AGTC Bioproducts Ltd 5 x 1 ml 180.00€
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Pub
682985328 Cellufine ETclean S , 500 ml Chisso 500 ml 3 455.55€ Pub
1104-200 Caspase 1 Substrate YVAD pNA Biovision 200 assays 311.85€
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Pub
K612-100 Free Fatty Acid Quantification Kit Biovision 100 assays 425.00€ Pub
CEM-1 Eosinophil - Mast Cell Stain Kit Scy tek 1 kit(s) 165.00€
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Pub
2021 T-cell proliferation grade chick type II collagen, 0.5 mg Chondrex 207.90€
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EACL-100 EnzyChrom™ Acetylcholine Assay Kit, Quantitative determination of acetylcholine by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 30 min. Kit size 100 tests. Detection limit Bioassays 100tests 345.00€ Pub
K113-200 Caspase 8 Colorimetric Assay Kit Biovision 200 assays 450.50€
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K629-100 Glutamate Assay Kit Biovision 100 assays 377.00€ Pub
024-A anti HCMV gB IgG1 (monoclonal) Produced against a HCMV infected cells. Virogen 100 µg 280.00€
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Pub
IC10-0510-25ml Rabbit Red Blood Cells 25ml Innovative Research INC 25ml 207.00€ Pub
20401 Cellufine MAX S h , 500 ml Chisso 500 ml 945.00€ Pub
683986326 Cellufine PB , 50 ml Chisso 50 ml 458.85€ Pub
1109-200 Caspase-4 Substrate LEVD-AFC; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K639-100 L Amino Acid Quantitation Kit Biovision 100 assays 333.50€ Pub
0801192 TBARS Assay Kit Zeptometrix 0.00€
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Pub
2022 TCBII T cell proliferation grade bovine type II collagen, 0.5 mg Chondrex 255.15€
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5524 Amplite™ Fluorimetric Thiol Quantitation Assay Kit *Green Fluorescence* AAT Bioquest 1 kit 202.00€
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Pub
DF2XPS 2X Happy Cell, DMEM-F12 Biocroi 25ml 175.00€ Pub
IQ391 Mouse Anti-Insulin-Like Growth Factor (IGF-1) Target Antigen Insulin-Like Growth Factor (IGF-1) Host Isotype Mouse IgG1 Application RIA or EIA assay development & studies of IGF-1 on frozen & paraf Imunquest 0.1ml (1mg/ml) 492.00€
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K401-60 ApoBrdU DNA Fragmentation Assay Kit Biovision 60 assays 533.00€
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Pub
M0271 MarkerGeneTM Membrane Fluidity Kit, Allows quantitative monitoring of membrane fluidity in cell membranes, micelles and vesicles using a lipophilic pyrene probe, 1 kit MarkerGene 197.00€
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Pub
y056129 Mouse Anti Pig B Cell Subset R PE Conjugate ABM Goods 100ug 361.20€
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DS-MB-01401 Mouse Anti-Rat Granulocytes Erythroid Cells Antibodies Ray Biotech 2 ml 650.00€ Pub
19805 Cellufine A 500 , 500 ml Chisso 500 ml 448.35€ Pub
670000335 Cellufine GH-25 Media AGTC Bioproducts Ltd 10 lt 0.00€
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Pub
1071-200 Caspase 2 Substrate VDVAD AFC Biovision 200 assays 311.85€
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Pub
K200-25 CaspSCREEN Caspase Screening Kit Biovision 25 assays 264.00€
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Pub
13511 Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
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Pub
21609 PhosphoWorks™ Luminometric ATP Assay Kit *Steady Glow* AAT Bioquest 1 kit 124.00€
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Pub
36341 Screen Quest™ Fluorimetric MDR Assay Kit *10 Plates* AAT Bioquest 1 kit 791.00€
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Pub
CBEL-Stat-SK Human Mouse Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA (2) ELISA Ray Biotech Two 96-Well Microplate Kit 607.00€ Pub
EPLP-100 EnzyChrom™ Phospholipd Assay Kit Bioassays 100 381.00€ Pub
K257-100 Cytochrome c Apoptosis Assay Kit Biovision 100 assays 484.00€
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K787-100 Alcohol Dehydrogenase Activity Assay Kit Biovision 100 assays 386.00€ Pub
PLF-960 ProPrep™ BAC 960 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group 10x96, 2.0ml cultures 1 501.00€ Pub
cAP-0002 Human Brain Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
19611-55 MC Cellufine A 200 , 5x5 ml Chisso 5x5 ml 257.25€
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Pub
1007-200 Caspase 3 Substrate DEVD AFC Biovision 200 assays 311.85€
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Pub
K138-9-25 Caspase Colorimetric Substrate Set II Plus Biovision 9 x 25 assays 854.00€ Pub
  P-4019-96  EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Fluorometric) (96 assays) EpigenTek 618.00€
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Pub
1106-1000 Jurkat Cell Extract (Uninduced)1 mg Biovision 1 mg 257.99€
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Pub
20352 Mouse anti human type II collagen IgG2a assay kit Chondrex 550.20€
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Pub
296-64401 Beta Amyloid (1 42) High Sensitivity ELISA Kit, Human Highly sensitive ELISA kit detecting beta amyloid (1 42). Detection sensitivity of the kits is about 10 times higher than that of the existing pro B-Bridge 96 tests 1 201.20€
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Pub
BC21011 Skin malignant tumor tissue array, 78 cases 78 cores (squamous carcinoma, adenocarcinoma, basal cell carcinoma, sarcoma, melanoma), replaced by BC21014 US Biomax 231.00€
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ECAT-100 EnzyChrom™ Catalase Assay Kit, Quantitative determination of catalase activity by colorimetric (570nm) or fluorometric (530nm 590nm) methods. Procedure 40 min. Kit size 100 tests. Detection limit 0 Bioassays 100tests 258.00€ Pub
K117-200 Caspase 2 Colorimetric Assay Kit Biovision 200 assays 486.50€
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K657-100 Glucose 6 Phosphate Assay Kit Biovision 100 assays 351.50€ Pub
M50266172 D (+) Glucose anhydrous cell culture tested CAS Number [50 99 7] Molekula 1KG 87.00€
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Pub
228-10986-3 Recombinant Canine ApoJ Clusterin [from HEK cells] Proteins Ray Biotech 1mg 0.00€
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Pub
KLS-MSCiPS-4 LumiSTEM 96 iPS MSC derived iPS cells Human Hemogenix 4 x 96-well plate 1 782.00€
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Pub
MOLBIO-SERV Molecular & Cellular Biology Services Molecular Biology Services Ray Biotech 0.00€
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Pub
1114-1000 Caspase 10 Substrate AEVD pNA1000 assays Biovision 1000 assays 579.29€
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Pub
OP-0002-1  EpiQuik Nuclear Extraction Kit (100 assays) EpigenTek 239.40€ Pub
1015T Mouse anti-human type I collagen IgG antibody assay kit, TMB Chondrex 425.25€
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Pub
2031 Mouse anti-chick type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
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Pub
7031-1 JNK Negative Control Cell Lysate1 mg Biovision 1 mg 258.00€
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Pub
DIGL-100 QuantiChrom™ Glucose Assay Kit Bioassays 100 229.00€ Pub
IRPAIKT-TOT Rat PAI-1 total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 575.00€ Pub
K4902-100 Adiponectin (mouse) Elisa Assay Kit Biovision 1 x 96 assays 943.00€ Pub
M0822 Green FAM Caspases 3 and 7 Assay Kit 100tests MarkerGene 651.00€
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Pub
Z5030021 Uric Acid Assay Kit Biochain 250 assays 619.00€
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Pub
IC05-0810-30ml Chicken Red Blood Cells, Packed 5% 30ml Innovative Research INC 30ml 207.00€ Pub
19875-51 MC Cellufine Chelate Media AGTC Bioproducts Ltd 5 x 1 ml 132.00€
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Pub
676944324 Cellufine Formyl , 10 ml Chisso 10 ml 255.15€
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Pub
1076-1000 Caspase 9 Substrate LEHD pNA1000 assays Biovision 1000 assays 579.29€
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Pub
K256-25 Mitochondria/Cytosol Fractionation Kit Biovision 25 assays 187.11€
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A00003.0025 CD20, B-Cell; Clone L26 Scy tek 25 ml Ready-to-use 281.00€
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Pub
15255 Amplite™ Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
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Pub
22604 Cell Explorer™ Fixed Cell Labeling Kit *Deep Red Fluorescence* AAT Bioquest 1 kit 164.00€
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Pub
4674-250-01 Cell Permeabilization Solution Trevigen 250 µl 297.15€
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Pub
ESOD-100 EnzyChrom™ Superoxide Dismutase Assay Kit Bioassays 100 tests 213.00€ Pub
K274-100 Total Antioxidant Capacity (TAC) Assay Kit100 assays Biovision 100 assays 325.08€ Pub
L00299 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC labeled POD) Genscript 1kit 851.55€
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RD191022200 ELISA Human , Clara Cell Protein Biovendor 96 wells (1 kit) 552.00€ Pub
cAP-0011 Human Synovial Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
19865-51 MC Cellufine A 800 , 5x1 ml Chisso 5x1 ml 115.50€ Pub
20601 Cellufine MAX Q-h Media AGTC Bioproducts Ltd 500 ml 1 488.00€ Pub
1015-1000 Annexin V PE Cy5 Reagent Biovision 1000 assays 945.50€
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Pub
K172-25 CaspGLOW Fluorescein Active Caspase 12 Staining Kit Biovision 25 assays 226.00€
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Pub
  P-4027-96  EpiQuik Global Acetyl Histone H4K16 Quantification Kit (Fluorometric) (96 assays) EpigenTek 606.00€
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Pub
11402 Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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Pub
20362T Mouse anti-mouse type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 485.10€
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Pub
3450-048-IK Directed In Vivo Angiogenesis Assay Inhibition Kit Trevigen 48 samples 611.00€
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Pub
bs-7605R-A555 Rabbit Anti-Cell death inducing protein C16orf5 Polyclonal Antibody, Alexa Fluor 555 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323.00€ Pub
EFDLC-100 EnzyFluo™ D-Lactate Assay Kit Bioassays 100 tests 285.00€ Pub
K123-200 Caspase 5 Colorimetric Assay Kit Biovision 200 assays 486.50€
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Pub
K710-100 Xanthine Oxidase Activity Assay Kit Biovision 100 assays 289.00€ Pub
NWK-TAC01 NWLSS™ TAC Peroxyl Assay (Total Antioxidant Capacity) Northwest Life Science 96 678.68€ Pub
297-57601 Leptin ELISA Kit, Rat Leptin, secreted from fatty cells, is known as a hormone controlling body fat by suppresssion of eating and increase of energy metabolism. It is reported, however, that expressio B-Bridge 96 tests 841.05€
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MD-11-0123 Mouse Anti-Human CD94 (Natural Killer Cells) Antibodies Ray Biotech 200 535.00€
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bs-2222R-PE Rabbit Anti-cellulase Polyclonal Antibody, PE Conjugated Bioss 100ug 369.00€ Pub
291-59201 GLP 1 ELISA Kit, Rat B-Bridge 96 assays 1 390.75€
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1032T Human monkey anti-bovine type I collagen IgG antibody assay kit, TMB Chondrex 528.36€
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20321 Mouse anti bovine type II collagen IgG1 assay kit Chondrex 503.00€
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80210 FAP assay kit BPS Bioscience 100 reactions 856.00€
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K106-200 Caspase 3 Colorimetric Assay Kit Biovision 200 assays 450.50€
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K607-100 Lactate Assay Kit Biovision 100 assays 425.00€ Pub
M0837 Magic RedTM (DEVD)2 Caspases 3 and 7 Assay Kit 25tests MarkerGene 257.00€
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0801027 CELLKINES MACROPHAGE COLONY STIMULATING FACTOR rM CSF 100000 Zeptometrix 1 0.00€
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IC05-3061-50ml Rat Red Blood Cells 50ml Innovative Research INC 50ml 339.00€ Pub
19908 Cellufine Q-500 Media AGTC Bioproducts Ltd 5 lt 0.00€
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681984324 Cellufine ETclean L , 10 ml Chisso 10 ml 255.15€ Pub
1102-200 Caspase 5 Substrate WEHD pNA200 assays Biovision 200 assays 287.28€
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K280-50 Mitochondrial DNA Isolation Kit50 assays Biovision 50 assays 390.29€ Pub
A00090.0025 CD5 (Mantel Cell Lymphoma Marker); 4C7 Scy tek 25 ml Ready-to-use 790.00€
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Pub
20050 Pluronic® F 127 *Cell culture tested * AAT Bioquest 10 g 55.00€
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22660 Cell Navigator™ F Actin Labeling Kit *Blue Fluorescence* AAT Bioquest 1 kit 202.00€
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50106 LSD 1 Fluorescent Assay kit BPS Bioscience 100 reactions 409.00€
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D2NO-100 QuantiChrom™ Nitric Oxide Assay Kit, Quantitative determination of nitric oxide by colorimetric (540nm) method Bioassays 100Tests 265.00€ Pub
IHTPAKT-TOT Total Human tPA Functional Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K335-100 SOD Activity Assay Kit Biovision 100 assays 279.00€ Pub
LC727 Lung large cell carcinoma tissue array, including TNM and clinical stage, 24 cases 72 cores US Biomax 231.00€
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Pub
STA-330 OxiSelect™ TBARS Assay Kit (MDA Quantitation) Cell Biolabs 200 assays 489.00€ Pub
cAP-0022 Human Bladder Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
195-15 MC Cellufine Phosphate Media AGTC Bioproducts Ltd 1 x 5 ml 348.00€
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202-15 MC Cellufine PB , 1x5 ml Chisso 1x5 ml 213.15€
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1069-1000 Caspase-6 Substrate VEID-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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Pub
K189-100 CaspGLOW Fluorescein Active Caspase 9 Staining Kit Biovision 100 assays 482.00€
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Pub
11956 Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
2044T Rat anti-rat type II collagen IgG antibody assay kit, TMB Chondrex 434.70€
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Pub
36006 Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence* AAT Bioquest 100 plates 3 131.00€
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Pub
BS02031 Esophagus squamous cell carcinoma (grade II) tissue array, 22 cases 63 cores, replaced by ES723 US Biomax 160.00€
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Pub
EKBD-100 EnzyChrom™ Ketone Body Assay Kit Bioassays 100 414.00€ Pub
K127-25 Caspase-4 Colorimetric Assay Kit Biovision 25 assays 201.00€
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K757-100 Glucose-6-Phosphate Dehydrogenase Activity Assay Kit Biovision 100 assays 357.00€ Pub
P-3003-2 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K9 EpigenTek 96 assays 770.00€ Pub
507-A anti CD16 NK cells, monocytes, granulocytes Virogen 100 µg 280.00€
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Y090765 Anti-bodywall muscle cells Monoclonal Antibody ABM Goods 100ul 265.00€
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1001-200 Annexin V FITC Reagent Biovision 200 assays 315.50€
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K120-50 Quick Apoptotic DNA Ladder Detection Kit Biovision 50 assays 338.00€
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Pub
  P-4011-96  EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Fluorometric) (96 assays) EpigenTek 618.00€
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Pub
1053 TCPI T cell proliferation grade porcine type I collagen Chondrex 0.5 mg 130.20€
ASK
Pub
20332 Mouse anti-porcine type II collagen IgG2a antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
AN1002424 Nycodenz, non ionic, non toxic, metabolically inert Cell Separation Media Accurate 500 gm. 892.50€ Pub
ELAC-100 EnzyChrom™ Lactose Assay Kit, Quantitative determination of lactose by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 30 min. Kit size 100 tests. Detection limit 6 µM. Shelf Bioassays 100tests 335.00€ Pub
K141-100 Cathepsin K Activity Assay Kit Biovision 100 assays 357.00€
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Pub
K762-100 Glutathione Peroxidase Activity Assay Kit Biovision 100 assays 357.00€ Pub
P-3016-096 EpiQuik In Situ Histone H3K9 Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
522-A anti IgM Heavy chain expressed on B cells Virogen 1 mg 1 328.00€
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19853 Cellufine Formyl Media AGTC Bioproducts Ltd 50 ml 468.00€
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1002-200 Annexin V Cy3 Reagent200 assays Biovision 200 assays 325.00€
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Pub
K129-100 Annexin V PE Cy5 Apoptosis Detection Kit Biovision 100 assays 378.50€
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Pub
  P-4014-48  EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
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Pub
1055 T-cell proliferation grade human type I collagen, 0.5 mg Chondrex 300.30€
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Pub
20333T Mouse anti-porcine type II collagen IgG2b antibody subtype assay kit, TMB Chondrex 400.05€
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2402-100 Jurkat cell Lysate (Camptothecin-treated) Biovision 100 μg 143.00€
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BBX24-CE Bullet Blender homogenizer, CE certified, for up to 24 tissue cell samples in snap cap microcentrifuge tubes. Next Advence 2 502.00€ Pub
EAPL-200 EnzyChrom™ Adipolysis Assay Kit Bioassays 200 338.00€ Pub
K114-25 Caspase 6 Fluorometric Assay Kit Biovision 25 assays 162.50€
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K635-100 Creatine Assay Kit Biovision 100 assays 357.00€ Pub
M1319 MarkerGeneTM Fluorescent Protease Assay Kit, Allows quick and simple measurement of general protease activity in biological samples. MarkerGene 261.00€
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Pub
22128 glial cells missing homolog 2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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Pub
IC100-0210-100ml Sheep Red Blood Cells, Packed 100% 100ml Innovative Research INC 100ml 257.00€ Pub
20500-51 MC Cellufine MAX Q-r Media AGTC Bioproducts Ltd 5 x 1 ml 180.00€
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683986335 Cellufine PB Media AGTC Bioproducts Ltd 10 lt 0.00€ Pub
1110-1000 Caspase 4 Substrate LEVD pNA1000 assays Biovision 1000 assays 579.29€
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K790-10000 StayBrite™ Highly Stable Luciferase Luciferin Reagent Biovision 10000 assays 4 876.00€ Pub
100-27 Human Beta-cell Attracting Chemokine 1 (CXCL13) BCA-1 CXCL13 Shenandoah Biotechnology, Inc. 5ug 113.40€ Pub
2024 TCRII T cell proliferation grade rat type II collagen Chondrex 0.5 mg 238.35€
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60345 PDE4D Assay Kit BPS Bioscience 96 reactions 491.00€
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DIAP-250 QuantiChrom™ BCP Albumin Assay Kit Bioassays 250 299.00€ Pub
IRAPKT036 Human Ceruloplasmin ELISA Kit (Cell Culture Media) Innovative Research INC 1 kit(96 Wells) 542.00€ Pub
K410-500 Phosphate Colorimetric Assay Kit500 assays Biovision 500 assays 0.00€ Pub
M0351 Thiamine galactoside, Cell Regulatory Substrate, 2mg MarkerGene 192.00€
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Y108140 BAFF (B cell activating factor)-R, C-Terminal Antibody ABM Goods 100 ug 1 015.35€
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DS-MB-02269 Rat Anti-Rat RT6.1 (Peripheral T Cells) Ray Biotech 2 mL 317.00€
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19846 Cellufine Sulfate Media AGTC Bioproducts Ltd 500 ml 2 964.00€ Pub
673980327 Cellufine A 800 , 100 ml Chisso 100 ml 234.15€ Pub
1072-200 Caspase-2 Substrate VDVAD-pNA; Appearance Liquid Biovision 200 assays 311.85€
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K202-25 Annexin V Cy3 Apoptosis Kit Plus Biovision 25 assays 180.50€
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13620 Amplite™ Colorimetric Sphingomyelinase Assay Kit AAT Bioquest 1 kit 212.00€
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21660 PhosphoWorks™ Fluorimetric Phosphate Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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36373 Screen Quest™ Fluorimetric ELISA cAMP Assay Kit AAT Bioquest 1 plate 280.00€
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CBEL-STAT6-1 Human Mouse Phospho-Stat 6 (Y641) Cell-Based ELISA (1) ELISA Ray Biotech One 96-Well Microplate Kit 347.00€ Pub
EPYR-100 EnzyChrom™ Pyruvate Assay Kit, Quantitative determination of pyruvate by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 20 min. Kit size 100 tests. Detection limit fluorimetr Bioassays 100tests 316.00€ Pub
K264-100 Glutathione (GSH/GSSG/Total) Assay Kit Biovision 100 assays 457.38€ Pub
K853-8 DiscoveryPak™ Stem Cell Fate Regulator Set II Biovision 8 Modulators 981.00€ Pub
PPO-200 ProPrep™ Omni 200 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group Up to 200 ml cultures 460.00€ Pub
cAP-0004GFP GFP Expressing Human Glomerular Microvascular Endothelial Cells AngoiPro 1.00 flask 830.00€ Pub
19800-55 MC Cellufine C-500 Media AGTC Bioproducts Ltd 5 x 5 ml 264.00€
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1008-200 Caspase-3 Substrate DEVD-pNA; Appearance Liquid Biovision 200 assays 311.85€
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K155-100 Caspase 5 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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  P-4022-48  EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Colorimetric) (48 assays) EpigenTek 358.00€
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1107-400 Jurkat Cell Extract (Induced) Biovision 400 ug 187.11€
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20354 Mouse anti-human type II collagen IgG3 antibody subtype assay kit, OPD Chondrex 442.05€
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3001 Rapid collagenase assay kit with type I collagen substrate Chondrex 454.65€
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ECLC-100 EnzyChrom™ Lactate Assay Kit Bioassays 100 371.00€ Pub
K118-25 Caspase 9 Fluorometric Assay Kit Biovision 25 assays 171.50€
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K671-100 Ascorbic Acid Assay Kit II (FRASC) Biovision 100 assays 406.00€ Pub
mAP-0133 Rat monoclonal anti mouse (T-cell Immunoglobulin Mucin-1) TIM-1 Anti-Mouse antibodies AngoiPro 100.00 ug 310.00€ Pub
228-11222-3 Recombinant Human PEDF [from HEK cells] Proteins Ray Biotech 100 1 172.00€ Pub
M1352 MarkerGene™ â Galactosidase Staining Kit, used to monitor and detect enzyme activity in stably or transiently transfected cells or transgenic tissues using X Gal, 1 kit MarkerGene 240.00€
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bs-2222R-A488 Rabbit Anti-cellulase Polyclonal Antibody, Alexa Fluor 488 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323.00€ Pub
1116-1000 Caspase 13 Substrate LEED AFC Biovision 1000 assays 613.31€
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P-1014B-48 Methylamp Global DNA Methylation Quantification Ultra Kit EpigenTek 48 assays 458.85€
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1022 Rat anti-bovine type I collagen IgG antibody assay kit, OPD Chondrex 373.80€ Pub
20312 Mouse anti chick type II collagen IgG2a assay kit Chondrex 497.70€
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7501-500-K HT Superoxide Dismutase Assay Kit Trevigen 480 tests 547.00€ Pub
DIMG-250 QuantiChrom™ Magnesium Assay Kit Bioassays 250 331.00€ Pub
K-3030-2 Kits for 96 well Plate Vacuum Manifold AccuPrep™ Plasmid Extraction kit Bioneer 96-well plate X 2 290.00€ Pub
K510-100 Nickel Assay Kit Biovision 100 assays 367.00€ Pub
M0826 Green FAM Caspase 8 Assay Kit 100tests MarkerGene 651.00€
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[302-C] DirectPCR Lysis Reagent (cell) Viagen cultured cells (100 ml) 215.25€
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IC05-0990-100ml Turkey Red Blood Cells 5% 100ml Innovative Research INC 100ml 240.00€ Pub
19881 Cellufine Phenyl Media AGTC Bioproducts Ltd 5 lt 0.00€
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676945335 Cellufine Amino Media AGTC Bioproducts Ltd 10 lt 0.00€
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1101-1000 Caspase-5 Substrate WEHD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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K266-100 Nuclear Cytosol Fractionation Kit Biovision 100 assays 377.00€
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A00024 CD45RO, T-Cell; Clone UCHL1 Scy tek 6 ml Ready-to-use 113.00€
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15261 Amplite™ Fluorimetric NADH Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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22615 Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation* AAT Bioquest 1 kit 202.00€
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4700-192-K Tankyrase 1 Colorimetric Activity Assay Trevigen 192 samples 1 342.00€
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CSB-E04776h Human Squamous Cell Carcinoma Antigen 2,SCCAg 2 ELISA Kit Cusabio 96tests 816.90€
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FA-1003 Stem Cell TF Activation Profiling Plate Array I Signosis 2011 6 537.00€
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LC1008 Non small cell lung carcinoma array for antibody screening, including TNM, clinical stage and pathology grade, 25 cases 100 cores US Biomax 231.00€
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SK802 Skin squamous cell carcinoma (SCC) tissue array, 80 cases 80 cores, no overlaps with SK801 US Biomax 231.00€
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cAP-0014 Human Prostate Microvascular Endothelial Cells AngoiPro 1.00 flask 570.00€ Pub
19900-55 MC Cellufine Phenyl , 5x5 ml Chisso 5x5 ml 342.30€
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20700-55 MC Cellufine MAX Phenyl Media AGTC Bioproducts Ltd 5 x 5 ml 516.00€
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1062-1000 Caspase 8 Substrate IETD AFC Biovision 1000 assays 613.31€
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K182-100 CaspGLOW Fluorescein Active Caspase 2 Staining Kit Biovision 100 assays 482.00€
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  P-4030-48  EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) (48 assays)  EpigenTek 358.00€
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11540 Amplite™ Fluorimetric Goat Anti Mouse IgG HRP Conjugate ELISA Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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20364T Mouse anti-mouse type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 487.20€
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bs-7605R-Cy5.5 Rabbit Anti-Cell death inducing protein C16orf5 Polyclonal Antibody, Cy5.5 conjugated, Isotype: IgG Sepax 100ug Lyophilized 268.00€
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EFNP-100 EnzyFluo™ NADP NADPH Assay Kit Bioassays 100 tests 301.00€ Pub
K124-25 Caspase 10 Fluorometric Assay Kit Biovision 25 assays 171.50€
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K723-100 Lipase Assay Kit II Biovision 100 assays 389.00€ Pub
OS208 High density (208 core) tissue microarray of osteosarcoma, chondrosarcoma, giant cell tumor of bone and normal tissue, with TNM data, 69 cases 208 cores US Biomax 456.00€
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42-654 Competent Cells for Cloning GC5 Competent Cells, pUC19 D Genesee 5 x 200ul/Unit 221.00€
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P10536 Rat Mesenchymal Stem Cells Smart Serology 5 x 10A5 cells/vial 351.00€ Pub
M0553 2 Chloro 4 nitrophenyl beta D lactoside, Fluorescent Substrate for Cellulases, 25mg MarkerGene 183.00€
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K101-100 Annexin V FITC Apoptosis Kit Biovision 100 assays 333.00€ Pub
P-1037-48  MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) (48 assays)  EpigenTek 527.99€
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1041 Human monkey anti-chick type I collagen IgA antibody assay kit, OPD Chondrex 528.36€
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20323 Mouse anti bovine type II collagen IgG2b assay kit Chondrex 497.70€
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935 Magic Red™ Caspases 3 & 7 Assay Kit ImmunoChemistry Technologies I.C.T 25 199.00€ Pub
DTBA-100 QuantiChrom™ TBARS Assay Kit Bioassays 100 135.00€ Pub
K110-25 Caspase 1 Fluorometric Assay Kit Biovision 25 assays 162.50€
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K616-100 Glucose and Sucrose Assay Kit Biovision 100 assays 386.00€ Pub
M0843 Magic RedTM (FR)2 Cathepsin L Assay Kit 25tests MarkerGene 257.00€
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M53340398 D (+) Cellobiose CAS Number [528 50 7] Molekula 25 G 110.00€
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IC10-0090-100ml Porcine Red Blood Cells, Packed 10% 100ml Innovative Research INC 100ml 257.00€ Pub
20301 Cellufine MAX S-r Media AGTC Bioproducts Ltd 500 ml 1 488.00€ Pub
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