Product name :Bovine prolactin-induced protein (PIP) ELISA kit, Species Bovine, Sample Type serum, plasma

Catalog Number :CSB-EL018020BO

Quantity :96T

Price :913.50 €

Pay now with :

Supplier :Cusabio

    Product Name :
    Bovine Prolactin-inducible protein(PIP) ELISA kit
    Alias:
    GCDFP-15, GCDFP15, GPIP4, prolactin-inducible protein
    Code:
    CSB-EL018020BO
    Size:
    96T
    Species:
    Bovine
    Target Name:
    prolactin-induced protein
    Abbreviation:
    PIP
    Sample Types:
    serum, plasma
    Detect Range:
    Request Information
    Sensitivity:
    Request Information
    Assay Time:
    1-5h
    Sample Volume:
    50-100ul
    Detection wavelengt:
    450 nm
   

 

Principle :     This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Specificty :     This assay has high sensitivity and excellent specificity for detection of Bovine PIP. No significant cross-reactivity or interference between Bovine PIP and analogues was observed.
    Precision :     Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
    Sample collection and storage :     Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
    Assay procedure :     Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
    Calculation of results :     Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PIP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

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228-10072-1 Recombinant Bovine Aprotinin Proteins Ray Biotech 1mg 147.00€
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IBV-N-1000ml-NaEDTA Bovine Plasma 1000ml Na EDTA Innovative Research INC 155.00€
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20022 IMBII SOL Immunization grade bovine type II collagen, 10 mg solution Chondrex 296.00€ Pub
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ASK
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ASK
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ASK
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228-11240-2 Recombinant Bovine PI3K alpha Proteins Ray Biotech 2.5 353.00€
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ASK
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ASK
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2062 Human monkey anti-bovine type II collagen IgA antibody assay kit, OPD Chondrex 518.81€
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1052 T-cell proliferation grade bovine type I collagen, 0.5 mg Chondrex 127.05€
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1012T Mouse anti-bovine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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2032T Mouse anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 387.45€
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228-10145-2 Native Bovine BSA Proteins Ray Biotech 50g 205.00€ Pub
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BSU-0100 Non-sterile bovine serum, 100ml Equitech 62.48€
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cAP-17 Bovine Type I Collagen Solution AngoiPro 100.00 mg 215.00€ Pub
228-11240-1 Recombinant Bovine PI3K alpha Proteins Ray Biotech 1 205.00€
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2042 Rat anti-bovine type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
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SFBIU-0100 Sterile filtered fetal bovine serum, low IgG,100ml Equitech 100 ml 184.80€
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228-10542-1 Recombinant Bovine GHBP Proteins Ray Biotech 5 147.00€
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228-10170-1 Native Bovine CAPN2 Proteins Ray Biotech 2 147.00€
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2052 Human monkey anti-bovine type II collagen IgG antibody assay kit, OPD Chondrex 518.81€
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SFBMDY-0100HI Sterile filtered fetal bovine serum, dialyzed, 100ml, heat inactivated Equitech 100 ml 233.10€
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SFBU-0050 Sterile filtered fetal bovine serum, 50ml Equitech 50 ml 121.80€
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1012 Mouse anti-bovine type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
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20021 Immunization grade bovine type II collagen, 10 mg Chondrex 222.00€
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1072 Immunization grade bovine type IX collagen, HM & LM, 5 mg lyophilized Chondrex 402.15€
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Pub
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Pub
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bs-2975R-PE Rabbit Anti-Noggin Polyclonal Antibody, PE Conjugated , 74kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Dog , Pig , Bovine , Horse , Sheep; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
bs-7607R-PE-Cy3 Rabbit Anti-CSNK1D Casein Kinase 1 delta Polyclonal Antibody, PE-Cy3 Conjugated , 46kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Rabbit , Pig , Dog , Sheep , Horse , Chicken; Applicat Bioss 100ug Lyophilized 530.00€ Pub
RAG001R ELISA Human , Angiopoietin-Like Protein 6 Biovendor 96 wells (1 kit) 665.00€ Pub
228-10244-2 Recombinant Human CKMB Type-2 Proteins Ray Biotech 50 292.00€
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228-11572-1 Recombinant Bovine Trypsin Ray Biotech 1 mg 201.00€ Pub
23113 zona pellucida binding protein isoform 1 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC SignalWay Antibody S.A.B 100ul 329.00€
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22263 calcium binding protein P22 antibody Source Rabbit Polyconal Ab Species Human Application WB IF SignalWay Antibody S.A.B 100ul 329.00€
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bs-7571R-PE Rabbit Anti-RPS3 Polyclonal Antibody, PE Conjugated , 24-27kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Pig , ZebraFish; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
22673 amyloid beta precursor protein-binding family B member 3 isoform a antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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228-10247-1 Recombinant Human CKMM Type-3 Proteins Ray Biotech 10 147.00€
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DS-MB-02436 Mouse Anti-Bovine TNF-alpha Antibodies Ray Biotech 0.5 mg 518.00€ Pub
DS-PB-00189 Rabbit Anti-Bovine Arginase Antibodies Ray Biotech 1 ml 485.00€ Pub
CSB-EL011649SH Sheep interleukin 2 receptor, alpha (IL2RA) ELISA kit SpeciesSheep Cusabio 96T 913.00€
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IR1-040N-500ml-NaCitrate Bovine Whole Blood 500ml Na Citrate Innovative Research INC 226.00€
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DS-MB-00882 Mouse Anti-Bovine CD8 beta Ray Biotech 2 mL 604.00€
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IRKFAP0139-5ug Interleukins Recombinant Bovine IL-1 beta Innovative Research INC 245.00€
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bs-6390R-PE-Cy3 Rabbit Anti-Necdin Polyclonal Antibody, PE-Cy3 Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog , Pig; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
bs-7559R-PE Rabbit Anti-PDGF AB+BB Polyclonal Antibody, PE Conjugated , 45kDa; Isotype IgG; Reactivity Mouse , Rat , Bovine , Human , Pig; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
2065 ELHAHII Human Monkey IgA anti human type II collagen ELISA kit Chondrex 550.20€
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bs-6850R-PE Rabbit Anti-TPST1 Polyclonal Antibody, PE Conjugated , 42-50kDa; Isotype IgG; Reactivity Human , Mouse , Bovine , Dog; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
22528 zinc finger protein 574 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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1004 ELRI ELISA grade rat type I collagen Chondrex 0.5 mg 164.85€
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bs-7577R-PE Rabbit Anti-SLC25A6 Polyclonal Antibody, PE Conjugated , 33kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Sheep , Chicken , Bovine , Dog , ZebraFish; Application Flow-Cyt(1 20-100), IF(1 50-200 Bioss 100ug Lyophilized 502.00€ Pub
DS-MB-01674 Mouse Anti-Infectious Bovine Rhinotracheitis Ray Biotech 200 396.00€ Pub
IRKTAH1188 Rat Retinol Binding Protein ELISA Kit Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
AK8213-1000 Bovine Insulin Akron 1g 869.00€ Pub
DS-MB-02624 Mouse Anti-Bovine CD25, RPE-labeled Ray Biotech 100 Tests 755.00€
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2015 ELHII ELISA grade human type II collagen Chondrex 0.5 mg 391.65€
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IR-BV-GF-50mg Bovine IgG 50mg Innovative Research INC 50mg 227.00€ Pub
MD-14-0370P Native Human Serum (Cystatin-C Free) Proteins Ray Biotech 50 mL 667.00€
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22544 ribosomal protein S3a antibody Source Rabbit Polyconal Ab Species Human Application WB IF SignalWay Antibody S.A.B 100ul 329.00€
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IHK-LMW Plasma Proteins: Kininogen, LMW, Human Plasma Innovative Research INC 0.1mg 518.00€ Pub
22830 G protein-coupled receptor 4 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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Y102596 Bovine Growth Hormone (BGH) ABM Goods 1 mg 453.00€
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DS-PB-01753 Rabbit Anti-Bovine S100 Antibodies Ray Biotech 1 ml 188.00€
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IC100-0410-100ml Bovine Red Blood Cells, Packed 100% 100ml Innovative Research INC 100ml 257.00€ Pub
DS-MB-02123 Mouse Anti-Bovine PI-3 Kinase p55 gamma Antibodies Ray Biotech 2 ml 411.00€
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bs-1027R-FITC Rabbit Anti-NSE ENO2 Polyclonal Antibody, FITC Conjugated, 48kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Dog , Bovine , Chicken; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
DS-MB-02653 Mouse Anti-Bovine IL-12 Antibodies Ray Biotech 0.5 mg 601.00€
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228-11273-3 Recombinant Bovine Placental Lactogen Ray Biotech 1 mg 2 068.00€
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IRKFAP0040-5ug Interleukins Recombinant Bovine IL-6 Innovative Research INC 245.00€
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228-10771-3 Bovine Holo Transferrin Ray Biotech 5 g 3 202.00€
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1024 ELRGRI Rat IgG anti rat type I collagen ELISA kit Chondrex 464.10€
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bs-7647R-PE Rabbit Anti-VDAC3 Polyclonal Antibody, PE Conjugated , 31kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Rabbit , Dog , Pig , ZebraFish; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
DS-PB-02982 Sheep Anti-Bovine DOPA Decarboxylase Antibodies Ray Biotech 0.1 ml 601.00€
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bs-7654R-PE-Cy3 Rabbit Anti-TIP30 Polyclonal Antibody, PE-Cy3 Conjugated , 27-30kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Bovine , Dog; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
FLGN11-M Mouse monoclonal anti-flagellin protein (Fla Flic BOR) IgG Species Reactivity: bacteria ADI 100 ul Product tipe: Antibodies 459.00€
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RD191073200R ELISA Human , Angiopoietin-Like Protein 4 Biovendor 96 wells (1 kit) 504.00€ Pub
ICTS-SER Cancer samples: Colorectal Cancer Serum-Adenocarcinoma Innovative Research INC Each 266.00€
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23123 hypothetical protein LOC27013 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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Y108214 Albumin, Bovine (BSA) -HRP Antibody ABM Goods 1 ml 567.00€
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DS-MB-02487 Mouse Anti-Bovine Ubiquitin Ray Biotech 100 717.00€
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ESD125 ELISA Sample Diluent Scy tek 125 ml 76.00€
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DS-PB-00385 Sheep Anti-Bovine Catalase Antibodies Ray Biotech 1 ml 386.00€ Pub
ITHYC-N Cancer Samples: Thyroid Carcinoma Plasma Innovative Research INC Each 302.00€
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129-10679 Mouse Anti-Bovine WC5 Ray Biotech 250 604.00€
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CSB-EL012972HU Human Lysosomal acid lipase cholesteryl ester hydrolase(LIPA) ELISA kit SpeciesHuman Cusabio 96T 815.85€
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IMLNM-N Cancer Samples: Melanoma Patient Plasma Innovative Research INC Each 302.00€
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IR1-040N-50ml-LiHeparin Bovine Whole Blood 50ml Li Heparin Innovative Research INC 185.00€
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DS-MB-01190 Mouse Anti-Bovine Dynein 74kDa Antibodies Ray Biotech 0.1 mg 535.00€
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IRKFAP0268-5ug Interleukins Recombinant Bovine IGF-1 Innovative Research INC 245.00€
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CAN-DHT-280 ELISA Human Serum Dihydrotestosterone (DHT) DBC 96 Wells/Kit 215.00€
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CSB-E12923h Human epididymal protein 4(HE4) ELISA Kit Cusabio 96tests 958.65€ Pub
2011 ELCII ELISA grade chick type II collagen, 0.5 mg Chondrex 255.15€ Pub
RH951CRP01R ELISA Rat , C-Reactive Protein, High Sensitivity Biovendor 96 wells (1 kit) 474.00€ Pub
Y052498 Anti-BMPR1B(Bone morphogenetic protein receptor type-1B) Antibody ABM Goods 100 μg 484.00€
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bs-6876R-PE-Cy3 Rabbit Anti-FMRP Polyclonal Antibody, PE-Cy3 Conjugated , 70kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Pig , Bovine , Dog , Chicken , Rabbit; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
1006 ELISA grade mouse type I collagen, 0.5 mg Chondrex 155.40€
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MCA4610T MOUSE ANTI HUMAN CD15, Product Type Monoclonal Antibody, Specificity CD15, Target Species Human, Host Mouse, Format Purified, Isotypes IgM, Applications C, F, P, Clone HI98 AbD Serotec 25 µg 148.05€ Pub
bs-7581R-PE-Cy3 Rabbit Anti-ZHX2 Alpha fetoprotein regulator 1 Polyclonal Antibody, PE-Cy3 Conjugated , 92kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Pig , Horse , Bovine , Dog , Chicken; Application Flow-C Bioss 100ug Lyophilized 530.00€ Pub
19251-24 Insulin from Bovine Pancreas[DISCONTINUED] Nacalai 100 mg 0.00€
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RSHAKRIN011HR ELISA Insulin (H-type) ,Mouse Biovendor 96 wells (1 kit) 563.00€ Pub
228-10069-3 Native Bovine Apo Transferrin Ray Biotech 5 g 3 202.00€ Pub
Y050248 Anti-BOVINE ALBUMIN Antibody ABM Goods 100ug 396.00€
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Y070006 Anti-Bovine Papillomavirus Purified Preparations (IgG1) Antibody ABM Goods 100μg 495.00€
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DS-MB-01869 Mouse Anti-Bovine MBP (aa 82-91) Antibodies Ray Biotech 0.25 ml 411.00€
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300-09971 Anti CEL Monoclonal Antibody (KNH 30) CEL is known to generate from protein modification by methylglyoxal . Mclellan et al. demonstrated that plasma methylglyoxal, which is believed to be generate fro B-Bridge 50 UG 958.65€
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DS-MB-02631 Mouse Anti-Bovine CD45 Ray Biotech 2 mL 604.00€
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2016 ELMsII ELISA grade mouse type II collagen, 0.5 mg Chondrex 611.10€
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IBAT-1mg Bovine Antithrombin 1mg Innovative Research INC 1mg 253.00€ Pub
228-11249-1 Recombinant PKA holoenzyme, type 1 alpha Proteins Ray Biotech 1 147.00€
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22033 ribosome binding protein 1 antibody Source Rabbit Polyconal Ab Species Human Application WB IHC IF SignalWay Antibody S.A.B 100ul 329.00€
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IRCTC-SER Cancer samples: Rectum Adenocarcinoma Serum Innovative Research INC Each 266.00€
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RD462183050 Leucine-Rich Alpha-2-Glycoprotein Serum , Canine Native protein Biovendor 0.05 mg 408.00€ Pub
228-10712-1 Recombinant HIV Type-O Envelope Proteins Ray Biotech 100 240.00€
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21332 Niban like protein 1(Ab 712) antibody Host Species Rabbit polyclonal SignalWay Antibody S.A.B 100 ug 197.00€
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