NMDAR, NR1, C2'
Rat and mouse.
Expected cross reactivity with human and bovine based upon sequence similarity.
N.B. Antibody reactivity and working conditions may vary between species., Mouse
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
This product specifically recognizes the 120kDa NR1 subunit of the NMDA (N-methyl-D-aspartate) receptor, containing the C2’ splice variant insert. This antibody does not recognize the NR1 subunit, where the C2’ splice variant insert is absent.
Receptors for NMDA (NMDAR) belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability.
The NR1 subunit has eight splice variants, arising from either the deletion or insertion of three exon cassettes within the N-terminal (N1) and C-terminal (C1, C2) NR1 domains. C2’ is a 22 amino acid sequence generated by elimination of the first stop codon, following splicing out of the C2 cassette. These splice variants differ in both their expression and pharmacological properties.
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Antisera to rat NMDA receptor, NR1 subunit, splice variant C2' were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Purified IgG prepared by affinity chromatography