Product name :Malachite Green Phosphate Assay Kit

Catalog Number :POMG-25H

Quantity :2500

Price :173.00 €

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Supplier :Bioassays

Malachite Green Phosphate Assay Kits (POMG-25H)
Rapid Colorimetric Phosphate Determination at 620 nm

DESCRIPTION
The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate
determination. Assays can be executed in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96- and 384-well plates for high-throughput screening of enzyme inhibitors.

KEY FEATURES

  • Reagent very stable. Due to our innovative formulation, no precipitation ofreagent occurs. Therefore no filtration of reagent is needed prior to assays,as is often required with other commercial kits
  • High sensitivity and wide detection range: detection of as little of 1.6pmoles of phosphate and useful range between 0.02 μM and 40 μM phosphate.
  • Fast and convenient: homogeneous “mix-and-measure” assay allows quantitation of free phosphate within 20 minutes.
  • Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers.

Robust and amenable to HTS: Z’ factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

APPLICATIONS

  • Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate.
  • Lipase Assays: liberation of phosphate from phospholipids Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
  • Quantitation of Phosphate in phospholipids, proteins and DNAs, etc.
  • Drug Discovery: high-throughput screen for phosphatase inhibitors.

KIT CONTENTS: 2,500 ASSAYS IN 96-WELL PLATE
Reagent A: 50 mL
Reagent B: 1 mL
Standard: 1mL 1 mM phosphate

Storage conditions. The reagents and standard are stable for one year when stored at 4°C.

Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.

PROCEDURE USING 96-WELL PLATE
Reagent Preparation.
Each assay requires 20 μL Working Reagent.
Prepare enough Working Reagent by mixing 100 vol of Reagent A and 1 vol of Reagent B (e.g. 5 mL Reagent A and 50 μL Reagent B). Working Reagent is stable for at least 1 day at room temperature.

Important: The reagent must be brought to room temperature before use.
Before each assay, it is important to check that all enzyme preparations and assay buffers do not contain free phosphate. This can be conveniently done by adding 20 μL of the Working Reagent to 80 μL sample solution.
The blank OD values at 620 nm should be lower than 0.2. If the OD readings are higher than 0.2, check water phosphate level. Double distilled water usually have OD readings lower than 0.1. Lab detergents may contain high levels of phosphate. Make sure that lab wares are free from contaminating phosphate after thorough washes.

1. Preparation of phosphate standards. Prepare a Premix solution containing 40 μM phosphate by pipetting 40 μL 1 mM phosphate standard to 960 μL distilled water or enzyme reaction buffer. Number the tubes. Dilute standards as shown in the following Table. Pipette 80 μL standard in duplicate into wells of a clear-bottom 96-well plate. Add blank controls containing water or reaction buffer only.

No Premix + H2O Final Vol(μL)  PhosphateConc (μM) pmoles Phosphate in 50 μL
1    200μL + 0μL         200                  40                                                 2,000
2    160μL + 40μL      200                   32                                                 1,600
3    120μL + 80μL      200                   24                                                 1,200
4      80μL + 120μL    200                   16                                                    800
5      60μL + 140μL    200                   12                                                    600
6      40μL + 160μL    200                     8                                                     400
7      20μL + 180μL    200                     4                                                     200
8        0μL + 200μL    200                      0                                                         0

2. Transfer 80 μL test samples into separate wells of the plate.
Note: in the case of enzyme reactions, the reaction may be terminated by adding a specific inhibitor, or can be stopped directly by the addition of the Working Reagent. Dilution of reaction mixture may be necessary prior to the assay (see General Considerations). For ATPase or GTPase assays, the ATP or GTP concentration should be lower than 0.25 mM. If the reaction mixture contains > 0.25mM ATP or GTP, dilute samples in distilled water. For example, if the ATPase reaction contained 1 mM ATP, at the end of reaction dilute reaction mixture 4-fold in water prior to the assay.
3. Add 20 μL of Working Reagent to each well. Mix gently by tapping the plate.
4. Incubate for 30 min at room temperature for color development.
5. Measure absorbance at 600 nm - 660nm (620 nm) on a plate reader.
For assays in 384-well plates, the procedures are the same, except that the volume of the standard and sample solution should be 40 μL and that of the Working Reagent should be 10 μL.
If precipitation occurs, perform a series dilution of sample in H2O, run the assay and determine the dilution factor from wells with no precipitation.
Repeat assays using diluted samples.
Enzyme reaction buffer. Because any exogenous free phosphate would interfere with the assay, it is important to ensure that the protein preparation, the reaction buffer and lab wares employed in the assay should not contain free phosphate. This can be conveniently checked by adding the Working Reagent to the buffer and measuring the color formation.

Liquid disposal. The assay mixture contains 0.4 M sulfuric acid. It is recommended that the waste liquid be neutralized with equal volume of 1 N NaOH prior to disposal.

DATA ANALYSIS
Plot OD620nm versus phosphate standard concentrations. Determine sample phosphate concentrations from the standard curve.

PUBLICATIONS
1. Guérette, D. et al (2007). Molecular evolution of type VI intermediate filament proteins. BMC Evolutionary Biology 2007, 7:164.
2. Green, M.L. et al (2005). Ethylene glycol induces hyperoxaluria without metabolic acidosis in rats. Am J Physiol Renal Physiol 289: F536–F543.
3. Saran, D. et al (2006). Multiple-turnover thio-ATP hydrolase and phospho-enzyme intermediate formation activities catalyzed by an RNA enzyme. Nucleic Acids Research, 34(11): 3201–3208.
4. Adkins, M.W. et al (2007). Chromatin disassembly from the PHO5 promoter Is essential for the recruitment of the general transcription machinery and coactivators. Mol. Cell. Biol. 27: 6372–6382.
 

TECHNICAL NOTES
The Malachite Green Phosphate Assay kits have been optimized and formulated to provide a sensitive, convenient and robust quantitation of free phosphate liberated from enzyme reactions and natural sources. Key features of the kits are as follows:


Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits.

Safe. Non-radioactive assay.

High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and 0.02 μM to 40 μM phosphate.

Fast and convenient: homogeneous “mix-and-measure” assay allows quantitation of free phosphate within 20 minutes.

 Compatible with routine laboratory and HTS formats: assays can beperformed in tubes or microplates, on spectrophotometers and plate readers.

Robust and amenable to HTS: Z’ factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

datasheet of product :Malachite Green Phosphate Assay Kit Malachite Green Phosphate Assay Kit

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80556 PARP6 Assay kit BPS Bioscience 32 reactions 892.00€
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Pub
ELDT-100 EnzyLight™ ADP ATP Ratio Assay Kit Bioassays 100 317.00€ Pub
K301-500 Quick Cell Proliferation Assay Kit Biovision 500 assays 230.00€ Pub
228-11412-2 Recombinant Human SCF KITLG Proteins Ray Biotech 10 205.00€ Pub
R9012LE-100g HyAgarose™ LE Agarose, Multi purpose, Green Agarose Product, 100g ACTGene 100g 83.00€
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Pub
1113-1000 Caspase-10 Substrate AEVD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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Pub
OP-0003-100 EpiQuik Whole Cell Extraction Kit EpigenTek 100 assays 213.15€
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Pub
2053 Human monkey anti-porcine type II collagen IgG antibody assay kit, OPD Chondrex 518.81€
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Pub
4999-5000 Enhanced Green Fluorescent Protein (EGFP) Biovision 5 mg 5 405.00€ Pub
EAPL-200 EnzyChrom™ Adipolysis Assay Kit Bioassays 200 338.00€ Pub
K124-400 Caspase 10 Fluorometric Assay Kit Biovision 400 assays 738.50€ Pub
K722-100 Lipase Activity Assay Kit Biovision 100 assays 357.00€ Pub
BIO-37042 DNA kits, Column-free Isolation: SureClean Bioline 1 x 5ml 62.00€
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Pub
1075-1000 Caspase 9 Substrate LEHD AFC Biovision 1000 assays 613.31€
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Pub
K256-100 Mitochondria/Cytosol Fractionation Kit Biovision 100 assays 384.62€
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Pub
1024 Rat anti-rat type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
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Pub
20334T Mouse anti-porcine type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 400.05€
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Pub
3471-096-K Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit Trevigen 96 tests 401.10€ Pub
DFOR-100 QuantiChrom™ Formaldehyde Assay Kit Bioassays 100 tests 299.00€ Pub
K110-400 Caspase 1 Fluorometric Assay Kit Biovision 400 assays 702.50€
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Pub
K610-100 Adipogenesis Assay Kit Biovision 100 assays 308.00€ Pub
1013-1000 Annexin V Cy5 Reagent1000 assays Biovision 1000 assays 937.00€
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Pub
K266-100 Nuclear/Cytosol Fractionation Kit Biovision 100 assays 395.01€
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Pub
1031T Human monkey anti-chick type I collagen IgG antibody assay kit, TMB Chondrex 528.36€
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Pub
20351 Mouse anti-human type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 442.05€
ASK
Pub
36006 Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence* AAT Bioquest 100 plates 3 131.00€
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Pub
DIAP-250 QuantiChrom™ BCP Albumin Assay Kit, Quantitative determination of albumin by bromcresol purple BCP method at 610nm Bioassays 250Tests 299.00€ Pub
K112-100 Caspase 8 Fluorometric Assay Kit Biovision 100 assays 315.50€
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Pub
K619-100 Fructose Assay Kit Biovision 100 assays 357.00€ Pub
M22187218 Fast Green FCF (C.I. 42053) CAS Number [2353 45 9] Molekula 25 G 120.00€
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Pub
1014-200 Annexin V PE Reagent Biovision 200 assays 315.50€
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Pub
K163-100 CaspSELECT Caspase 3 Immunoassay Kit Biovision 100 assays 649.00€
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Pub
  P-4029-48  EpiQuik Global Acetyl Histone H4K12 Quantification Kit (Fluorometric) (48 assays) EpigenTek 373.00€
ASK
Pub
PBD010 Phosphate Buffered Saline (10x) pH 7.4 Scy tek 10 L 211.00€
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Pub
22830 Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm* AAT Bioquest 1 kit 202.00€
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Pub
AS06185 SPS | sucrose phosphate synthase, maize Agrisera 100 ul 337.00€
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Pub
FA-0001 Transcription Factors: NFkB Filter Assay Signosis 2011 96 449.00€ Pub
K370-100 Ammonia Assay Kit Biovision 100 assays 367.00€ Pub
T344500 6-Thioinosine Phosphate C10H13N4O7PS CAS: 53-83-8 TRC 2.5 mg 232.00€ Pub
1117-200 Caspase 12 Substrate ATAD AFC Biovision 200 assays 415.80€
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Pub
P-1037-48 MethylFlash Hydroxymethylated DNA Quantification Kit Fluorometric EpigenTek 48 assays 507.82€
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Pub
13520 MMP Green™ substrate AAT Bioquest 1 mg 280.00€
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Pub
22253 CytoTell™ Green AAT Bioquest 500 Tests 124.00€
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Pub
60320 PDE2A Assay Kit BPS Bioscience 96 reactions 491.00€
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Pub
ECTX-100 EnzyLight™ Cytotoxicity Assay Kit Bioassays 100 220.00€ Pub
K773-100 Catalase Activity Assay Kit Biovision 100 assays 289.00€ Pub
P-3016-096 EpiQuik In Situ Histone H3K9 Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
1102-200 Caspase-5 Substrate WEHD-pNA; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K404-60 ApoBrdU Red DNA Fragmentation Kit Biovision 60 assays 533.00€
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Pub
11305 Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit AAT Bioquest 1 kit 202.00€
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Pub
20361 Mouse anti mouse type II collagen IgG1 assay kit Chondrex 603.75€ Pub
36350 Screen Quest™ Colorimetric Chloride Channel Assay Kit AAT Bioquest 10 Plates 635.00€
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Pub
DIOX-250 QuantiChrom™ Peroxide Assay Kit Bioassays 250 285.00€ Pub
K116-100 Caspase 2 Fluorometric Assay Kit Biovision 100 assays 342.50€
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Pub
K642-100 L Carnitine Assay Kit Biovision 100 assays 405.50€ Pub
M48611764 Potassium dihydrogen phosphate (Monobasic) CAS Number [7778 77 0] Molekula 1KG 87.00€
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Pub
1063-200 Caspase 8 Substrate IETD pNA Biovision 200 assays 311.85€
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Pub
K189-25 CaspGLOW Fluorescein Active Caspase 9 Staining Kit Biovision 25 assays 200.00€
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Pub
TGG125 Trichrome Stain Solution (Green) Scy tek 125 ml 84.00€
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Pub
20315 Mouse anti-chick type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
291-58601 Alkaline Phospatase (ALP) Assay B-Bridge 900 tests 370.27€
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Pub
CQLD-100 QuantiChrom™ LDH Cytotoxicity Assay Kit Bioassays 100 tests 85.00€ Pub
IMTPAKT Active Mouse tPA Functional Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K412-500 Alkaline Phosphatase Colorimetric Assay Kit Biovision 500 assays 379.00€
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Pub
M0838 Magic RedTM (DEVD)2 Caspases 3 and 7 Assay Kit 100tests MarkerGene 560.00€
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Pub
1001-1000 Annexin V FITC Reagent Biovision 1000 assays 945.50€
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Pub
K104-100 Annexin V EGFP Apoptosis Kit Biovision 100 assays 333.00€
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Pub
  P-4013-48  EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Fluorometric) (48 assays) EpigenTek 370.00€
ASK
Pub
ABD999 Normal Antibody Diluent (Phosphate Buffered) without BSA Scy tek 1000 ml 106.00€
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Pub
15255 Amplite™ Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
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Pub
7513-500-K HT Glutathione Reductase Assay Kit Trevigen 480 tests 538.00€
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Pub
EGLT-100 EnzyChrom™ Glutamate Assay Kit Bioassays 100 360.00€ Pub
K262-200 Nitric Oxide Colorimetric Assay Kit Biovision 200 assays 357.00€ Pub
PP-307S-488 Atto488 PCR Labeling Master, S pack Green fluorescent DNA labeling by PCR Jena Bioscience 10reactions (20 µl each) 135.00€
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Pub
1109-1000 Caspase 4 Substrate LEVD AFC Biovision 1000 assays 613.31€
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Pub
K793-100 MMP-3 Inhibitor Screening Kit Biovision 100 assays 503.00€ Pub
11610 MUP, disodium salt [4 Methylumbelliferyl phosphate, disodium salt] *UltraPure grade* AAT Bioquest 25 mg 108.00€
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Pub
2042T Rat anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 384.30€
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Pub
4676-096-K Universal Chemiluminescent PARP Assay Kit w Histone Coated Strip wells Trevigen 96 Samples 484.00€ Pub
E2HL-100 EnzyChrom™ AF HDL and LDL VLDL Assay Kit Bioassays 100 425.00€ Pub
K122-100 Caspase 5 Fluorometric Assay Kit Biovision 100 assays 342.00€
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Pub
K686-100 Glucose Assay Kit II Biovision 100 assays 357.00€ Pub
M68034770 Janus green B (C.I. 11050) CAS Number [2869 83 2] Molekula 5 G 104.00€
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Pub
1071-1000 Caspase 2 Substrate VDVAD AFC1000 assays Biovision 1000 assays 541.49€
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Pub
K201-25 Annexin V FITC Apoptosis Kit Plus Biovision 25 assays 180.50€
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Pub
1013 Mouse anti-porcine type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
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Pub
2033 Mouse anti-porcine type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
ASK
Pub
3450-048-IK Directed In Vivo Angiogenesis Assay Inhibition Kit Trevigen 48 samples 611.00€
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Pub
DALP-250 QuantiChrom™ Alkaline Phosphatase Assay Kit Bioassays 250 299.00€ Pub
K105-100 Caspase 3 Fluorometric Assay Kit Biovision 100 assays 315.50€ Pub
K555-100 Hydroxyproline Assay Kit Biovision 100 assays 406.00€ Pub
M1281 Rhodamine 110, Red fluorophore standard used in enzyme assay systems, 25mg MarkerGene 89.00€
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Pub
1007-1000 Caspase 3 Substrate DEVD AFC1000 assays Biovision 1000 assays 541.00€
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Pub
K133-9-25 Caspase Fluorometric Substrate Set II Biovision 9 x 25 assays 687.00€
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Pub
  P-4021-48  EpiQuik Global Acetyl Histone H3K56 Quantification Kit (Fluorometric) (48 assays) EpigenTek 370.00€
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Pub
LGB500 Light Green S.F. Yellowish Solution Scy tek 500 ml 97.00€
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Pub
1757-100 Sitagliptin Phosphate Monohydrate; Appearance White solid Biovision 100 mg 103.95€ Pub
22810 Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry* AAT Bioquest 1 kit 202.00€
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Pub
A105020 Abacavir 5’-Phosphate C14H19N6O4P CAS: 136470-77-4 TRC 1 mg 277.00€
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Pub
ENH3-100 EnzyChrom™ Ammonia Assay Kit, Quantitative determination of ammonia ammonium concentration by colorimetric (340nm) or fluorimetric (360nm 450nm) methods. Bioassays 100Tests 271.00€ Pub
K311-400 LDH Cytotoxicity Assay Kit Biovision 400 assays 261.50€ Pub
DI0207 KITLG & FLT3LG Protein Protein Interaction Antibody Pair Abnova 1 Set 765.00€
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Pub
K-6203 AccuPower GreenStar qPCR PreMix Exicycler 96 well plate, 1 plate 96 tests , optical film included Bioneer 50 ul 184.00€ Pub
1114-1000 Caspase-10 Substrate AEVD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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Pub
P-1014-96  Methylamp Global DNA Methylation Quantification Kit (96 assays) EpigenTek 808.50€
ASK
Pub
12519 Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* AAT Bioquest 10 plates 358.00€
ASK
Pub
2062 Human monkey anti-bovine type II collagen IgA antibody assay kit, OPD Chondrex 518.81€
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Pub
510EZPANG-10 LATERAL FLOW ASSAY EZ PANGASIUS™ 10 Test Kit Elisa Technologies 1 kit 245.00€
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Pub
EASTR-100 EnzyChrom™ Aspartate Transaminase Assay Kit, Quantitative determination of aspartate transaminase activity at 340nm. Procedure 10 min. Kit size 100 tests. Detection limit 2 U L. Shelf life 6 month Bioassays 100tests 296.00€ Pub
K126-200 Caspase 4 Fluorometric Assay Kit Biovision 100 assays 495.50€
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K729-100 Glutamate Dehydrogenase Assay Kit Biovision 100 assays 357.00€ Pub
MSP20 Rapid Microplate Assay Kit for PDH Activity and Quantity (Combo) ACR 96 Tests 830.55€ Pub
1076-1000 Caspase-9 Substrate LEHD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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Pub
12536 Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* AAT Bioquest 10 plates 791.00€
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Pub
21508 Thiolite™ Green AAT Bioquest 5 mg 163.00€
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Pub
52006 Methyltransferase SUV39H1 Assay Kit BPS Bioscience 100 reactions 530.00€
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Pub
ECAT-100 EnzyChrom™ Catalase Assay Kit Bioassays 100 274.00€ Pub
K127-25 Caspase-4 Colorimetric Assay Kit Biovision 25 assays 201.00€
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Pub
K753-100 Aspartate Aminotransferase (AST or SGOT) Activity Assay Kit Biovision 100 assays 357.00€ Pub
NWK-TAC01 NWLSS™ TAC Peroxyl Assay (Total Antioxidant Capacity) Northwest Life Science 96 678.68€ Pub
1076-200 Caspase 9 Substrate LEHD pNA200 assays Biovision 200 assays 287.28€
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Pub
K267-50 Cytosol Particulate Separation Kit Biovision 50 assays 308.00€ Pub
1035 Human monkey anti-human type I collagen IgG antibody assay kit, OPD Chondrex 599.34€
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Pub
20352T Mouse anti-human type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 442.05€
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Pub
36309 Screen Quest™ Fluo 8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 100 Plates 1 571.00€
ASK
Pub
DICL-250 QuantiChrom™ Chloride Assay Kit Bioassays 250 265.00€ Pub
K113-200 Caspase 8 Colorimetric Assay Kit Biovision 200 assays 450.50€
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Pub
K626-100 Sucrose Assay Kit Biovision 100 assays 357.00€ Pub
M31140436 Methyl green (C.I.42590) CAS Number [7114 03 6] Molekula 25 G 120.00€
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Pub
1015-200 Annexin V PE Cy5 Reagent200 assays Biovision 200 assays 352.00€
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Pub
K180-100 CaspGLOW Fluorescein Active Caspase Staining Kit Biovision 100 assays 482.00€
ASK
Pub
  P-4032-96  EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) (96 assays) EpigenTek 606.00€
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Pub
PBE999 Phosphate Buffered Saline + Tween 20 (10x) pH 7.4 Scy tek 1000 ml 92.00€
ASK
Pub
20311 Mouse anti-chick type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
IHPAIKT Active human PAI-1 functional assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K380-250 Calcium Colorimetric Assay Kit Biovision 250 assays 289.00€
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Pub
  OP-0004-1  CytoX Red Cell Proliferation Cytotoxicity Assay Kit (3x96 assays) EpigenTek 174.00€
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Pub
32000-1 Live Bacterial Gram Stain Kit 200 assays Biotium 1 KIT 177.45€ Pub
P-1040-96 MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (fluorometric) (96 assays) EpigenTek 649.00€ Pub
13602 FAM cAMP PDE IV substrate *Green fluorescence* AAT Bioquest 0.5 umol 280.00€
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Pub
22615 Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation* AAT Bioquest 1 kit 202.00€
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Pub
684987324 Cellufine Phosphate Media AGTC Bioproducts Ltd 10 ml 348.00€
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Pub
EERK-100 EnzyFluo™ ERK Phosphorylation Assay Kit Bioassays 100 tests 381.00€ Pub
K783-100 MMP-3 Activity Assay Kit Biovision 100 assays 425.00€ Pub
P-4008-96 EpiQuik Global Histone H3 Acetylation Assay Kit 96 assays EpigenTek 96 assays 769.65€
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Pub
1103-1000 Caspase 1 Substrate YVAD AFC1000 assays Biovision 1000 assays 541.49€
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Pub
K612-100 Free Fatty Acid Quantification Kit Biovision 100 assays 425.00€ Pub
11403 Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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Pub
20363 Mouse anti-mouse type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 485.10€
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Pub
36374 Screen Quest™ Fluorimetric ELISA cAMP Assay Kit AAT Bioquest 10 plates 1 571.00€
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Pub
DIZN-250 QuantiChrom™ Zinc Assay Kit Bioassays 250 422.00€ Pub
K117-200 Caspase 2 Colorimetric Assay Kit Biovision 200 assays 486.50€
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Pub
K653-100 Formate Assay Kit Biovision 100 assays 435.00€ Pub
M56441018 Piperazine phosphate CAS Number [14538 56 8] Molekula 500 G 895.00€
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Pub
1069-200 Caspase-6 Substrate VEID-AFC; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K193-100 Caspase 3 Staining Kit, CaspGLOW Red Biovision 100 assays 400.00€
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Pub
0801192 TBARS Assay Kit Zeptometrix 0.00€
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Pub
20322 Mouse anti-bovine type II collagen IgG2a antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
3001 Rapid collagenase assay kit with type I collagen substrate Chondrex 454.65€
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Pub
IPOPAIKT Active Porcine PAI-1 Functional Assay Innovative Research INC 1 kit(96 Wells) 575.00€ Pub
K431-40 KinaseSTAR JNK Activity Assay Kit Biovision 40 assays 687.00€ Pub
M0844 Magic RedTM (FR)2 Cathepsin L Assay Kit 100tests MarkerGene 560.00€
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Pub
1002-1000 Annexin V Cy3 Reagent1000 assays Biovision 1000 assays 891.00€
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Pub
K109-400 Annexin V Biotin Apoptosis Kit Biovision 400 assays 702.50€
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Pub
  P-4015-96  EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Fluorometric) (96 assays) EpigenTek 618.00€
ASK
Pub
ATG125 Primary Antibody Diluent (Tris, Green) Scy tek 125 ml 71.00€
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Pub
15261 Amplite™ Fluorimetric NADH Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
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Pub
22794 Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Optimized for Microplate Readers* AAT Bioquest 1 kit 215.00€
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Pub
80208 DPP8 assay kit BPS Bioscience 100 reactions 437.00€
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Pub
EIVT-100 EnzyChrom™ Invertase Assay Kit Bioassays 100 tests 317.00€ Pub
K265-200 Hydrogen Peroxide Assay Kit Biovision 200 assays 260.00€ Pub
228-11410-2 Recombinant Mouse SCF KITLG Proteins Ray Biotech 10 205.00€
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Pub
QFPR-200 QuantiFluo™ Protein Assay Kit Bioassays 200 tests 109.00€ Pub
1110-1000 Caspase-4 Substrate LEVD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
ASK
Pub
K812-1000 BCA Protein Quantitation Kit Biovision 1000 assays 240.00€ Pub
11620 pNPP [4 Nitrophenyl phosphate, disodium salt *UltraPure Grade* AAT Bioquest 1 g 124.00€
ASK
Pub
2045 Rat anti-human type II collagen IgG antibody assay kit, OPD Chondrex 434.70€
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Pub
4701-096-K Tankyrase 1 Chemiluminescent Activity Assay Trevigen 96 samples 996.00€ Pub
EACL-100 EnzyChrom™ Acetylcholine Assay Kit Bioassays 100 371.00€ Pub
K123-200 Caspase 5 Colorimetric Assay Kit Biovision 200 assays 486.50€
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Pub
K707-400 PhosphoSeek™ PTP1B Assay Kit Biovision 400 Assays 640.00€ Pub
MD-14-0252P Recombinant Human SCF KITLG Proteins Ray Biotech 10 309.00€
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Pub
1072-1000 Caspase 2 Substrate VDVAD pNA Biovision 1000 assays 634.10€
ASK
Pub
K203-100 Annexin V PE Apoptosis Kit Plus Biovision 100 assays 333.50€
ASK
Pub
1021 Rat anti-chick type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
ASK
Pub
20332 Mouse anti porcine type II collagen IgG2a assay kit Chondrex 497.70€
ASK
Pub
3457-096-K Cultrex 96 Well Collagen I Cell Invasion Assay Trevigen 96 samples 401.00€ Pub
DATG-200 QuantiChrom™ ATPase GTPase Assay Kit Bioassays 200 345.00€ Pub
K106-200 Caspase 3 Colorimetric Assay Kit Biovision 200 assays 450.50€
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Pub
K601-100 CETP Activity Assay Kit Biovision 100 assays 464.00€
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Pub
M1405 MarkerGene™ Cellular Senescence Microtiterplate Assay Kit, This kit uses the high sensitivity substrate fluorescein di â D galactopyranoside (FDG) to quantify senescent cells, 1 kit MarkerGene 316.00€
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Pub
1008-1000 Caspase 3 Substrate DEVD pNA Biovision 1000 assays 613.31€
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Pub
K138-9-25 Caspase Colorimetric Substrate Set II Plus Biovision 9 x 25 assays 854.00€ Pub
  P-4023-96  EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Fluorometric) (96 assays) EpigenTek 606.00€
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Pub
MGP125 Methyl Green Pyronin Solution Scy tek 125 ml 86.00€
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Pub
195-15 MC Cellufine Phosphate , 1x5 ml Chisso 1x5 ml 213.15€
ASK
Pub
22817 Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence* AAT Bioquest 100 Tests 202.00€
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Pub
A280502 Adenosine 2’,3’-Cyclic Phosphate-13C5 Triethylammonium Salt C1113C5H27N6O6P CAS: TRC 0.5 mg 618.00€
ASK
Pub
EPLP-100 EnzyChrom™ Phospholipd Assay Kit Bioassays 100 381.00€ Pub
K331-100 HDAC Colorimetric Activity Assay Kit Biovision 100 assays 357.00€
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Pub
M0542 Rhodamine 123, Green Fluorescent Dye, 25mg MarkerGene 117.00€
ASK
Pub
K-6213 AccuPower GreenStar qPCR PreMix Exicycler 96 well plate, 1 plate 96 tests , optical film included Bioneer 20 ul 128.00€ Pub
1114-200 Caspase 10 Substrate AEVD pNA200 assays Biovision 200 assays 287.28€
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Pub
P-1020-48 FitAmp General DNA Quantification Kit EpigenTek 48 assays 160.65€
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Pub
NPB500 Naphthol Phosphate Buffer Scy tek 500 ml 155.00€
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Pub
20010-2 SYBR Green I Gel Staining Solution, 10000x Lumiprob 2 x 1 mL 347.00€
ASK
Pub
A629235 erythro-ω-Amino Sphingosine Biotinamide Phosphate C28H53N4O7PS CAS: TRC 10 mg 5 575.00€
ASK
Pub
EPYR-100 EnzyChrom™ Pyruvate Assay Kit, Quantitative determination of pyruvate by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 20 min. Kit size 100 tests. Detection limit fluorimetr Bioassays 100tests 316.00€ Pub
K335-100 SOD Activity Assay Kit 100 assays Biovision 100 assays 230.58€ Pub
SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.37€ Pub
1116-200 Caspase 13 Substrate LEED AFC Biovision 200 assays 311.85€
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Pub
P-1034-48  MethylFlash Methylated DNA Quantification Kit (Colorimetric) (48 assays)  EpigenTek 458.85€ Pub
13502 Amplite™ Fluorimetric Caspase 3 7 Assay Kit *Blue Fluorescence* AAT Bioquest 1 kit 130.00€
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Pub
21655 PhosphoWorks™ Fluorimetric ADP Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
ASK
Pub
52191 4x HMT Assay Buffer 5 Biotech support group 30 ml 200.00€
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Pub
ECIT-100 EnzyChrom™ Citrate Assay Kit Bioassays 100 tests 261.00€ Pub
K140-100 Cathepsin B Activity Assay Kit Biovision 100 assays 357.00€
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Pub
K761-200 Glutathione Reductase Activity Assay Kit Biovision 200 assays 357.00€ Pub
P-3003-1 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K9 EpigenTek 48 assays 461.00€ Pub
1101-200 Caspase-5 Substrate WEHD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
K280-50 Mitochondrial DNA Isolation Kit Biovision 50 assays 425.00€ Pub
1045 Human monkey anti-human type I collagen IgA antibody assay kit, OPD Chondrex 599.34€
ASK
Pub
20354T Mouse anti-human type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 442.05€
ASK
Pub
36336 Cal-520™ Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 10 plates 436.00€
ASK
Pub
DIGL-100 QuantiChrom™ Glucose Assay Kit Bioassays 100 229.00€ Pub
K114-25 Caspase 6 Fluorometric Assay Kit Biovision 25 assays 162.50€
ASK
Pub
K631-100 D-Sorbitol Colorimetric Assay Kit Biovision 100 assays 425.00€ Pub
M38655651 Tetrabutylammonium phosphate CAS Number [5574 97 0] Molekula 5 G 166.00€
ASK
Pub
1062-200 Caspase 8 Substrate IETD AFC Biovision 200 assays 311.85€
ASK
Pub
K183-100 CaspGLOW Fluorescein Active Caspase 3 Staining Kit Biovision 100 assays 482.00€
ASK
Pub
PBS500 Phosphate Buffered Saline (25x) pH 7.6 Scy tek 500 ml 69.00€
ASK
Pub
20312T Mouse anti-chick type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 400.05€
ASK
Pub
23000 Cell Meter™ Autophagy Assay Kit AAT Bioquest 1 kit 202.00€
ASK
Pub
BUF036A 10X PHOSPHATE BUFFERED SALINE, Product Type Accessory Reagent, Specificity 10X PHOSPHATE BUFFERED SALINE, Target Species , Host N A, Format Concentrate, Isotypes , Applications IY, Clone AbD Serotec 1 LITRE 266.00€ Pub
IHUPAKT Active Human uPA Functional Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K402-50 ApoDIRECT DNA Fragmentation Assay Kit Biovision 50 assays 533.00€
ASK
Pub
  OP-0005-3  CytoX Violet Cell Proliferation Cytotoxicity Assay Kit (20x96 assays) EpigenTek 319.00€
ASK
Pub
K-1050 SilverStar® Staining Kit 20 assays Bioneer 20 assays 323.00€
ASK
Pub
  P-4010-48  EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
ASK
Pub
13622 Amplite™ Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
22780 Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit AAT Bioquest 1 kit 163.00€
ASK
Pub
685987330 Cellufine Phosphate Media AGTC Bioproducts Ltd 5 lt 0.00€
ASK
Pub
EFND-100 EnzyFluo™ NAD NADH Assay Kit Bioassays 100 tests 301.00€ Pub
K254-1000 ATP Cell Viability Assay Kit, ApoSENSOR Biovision 1000 assays 667.00€
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Pub
K791-1000 StayBrite™ Highly Stable ATP Assay Kit Biovision 1000 assays 923.00€ Pub
1104-1000 Caspase 1 Substrate YVAD pNA Biovision 1000 assays 613.31€
ASK
Pub
K639-100 L Amino Acid Quantitation Kit Biovision 100 assays 333.50€ Pub
11541 Amplite™ Fluorimetric Goat Anti Rabbit IgG HRP Conjugate ELISA Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
20365 Mouse anti-mouse type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 485.10€
ASK
Pub
36401 Cal-520™ PBX Calcium Assay Kit *Optimized for Probenecid-Sensitive Cell Lines* AAT Bioquest 100 plates 3 911.00€
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DSLA-100 QuantiChrom™ Sialic Acid Assay Kit Bioassays 100 422.00€ Pub
K118-25 Caspase 9 Fluorometric Assay Kit Biovision 25 assays 171.50€
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K661-100 Ascorbic Acid Assay Kit Biovision 100 assays 357.00€ Pub
M62293617 di Potassium hydrogen phosphate anhydrous CAS Number [7758 11 4] Molekula 1KG 88.00€
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1070-1000 Caspase 6 Substrate VEID pNA1000 assays Biovision 1000 assays 541.49€
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K198-25 CaspGLOW Red Active Caspase 8 Staining Kit Biovision 25 assays 200.00€
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10056 Amplite™ Fluorimetric Glutathione GSH GSSG Ratio Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 180.00€
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20323T Mouse anti-bovine type II collagen IgG2b antibody subtype assay kit, TMB Chondrex 400.05€
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30029 NUC VIEW 488 CASPASE 3 ASSAY KIT Biotium 1 KIT 496.65€ Pub
D2NO-100 QuantiChrom™ Nitric Oxide Assay Kit, Quantitative determination of nitric oxide by colorimetric (540nm) method Bioassays 100Tests 265.00€ Pub
IRPAIKT-TOT Rat PAI-1 total antigen assay ELISA kit Innovative Research INC 1 kit(96 Wells) 575.00€ Pub
K4903-100 Adiponectin (rat) Elisa Assay Kit Biovision 1 x 96 assays 943.00€ Pub
M1214 MarkerGeneTM Long Wavelength Fluorescent Lipase Assay Kit, Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the substrate resorufin oleate, 1 kit MarkerGene 380.00€ Pub
1004-1000 Annexin V EGFP Reagent Biovision 1000 assays 945.50€
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K129-100 Annexin V PE Cy5 Apoptosis Detection Kit Biovision 100 assays 378.50€
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  P-4018-48  EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
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HAB999 HRP Stabilizing Diluent (Phosphate Buffered) without BSA Scy tek 1000 ml 139.00€
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1617-5 Canertinib (CI-1033, PD-183805); Appearance Off-white to pale green solid Biovision 5 mg 145.53€ Pub
80553 PARP3 Assay kit BPS Bioscience 96 reactions 824.00€
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ELAC-100 EnzyChrom™ Lactose Assay Kit, Quantitative determination of lactose by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 30 min. Kit size 100 tests. Detection limit 6 µM. Shelf Bioassays 100tests 335.00€ Pub
K301-2500 Quick Cell Proliferation Assay Kit Biovision 2500 assays 435.00€ Pub
228-11412-1 Recombinant Human SCF KITLG Proteins Ray Biotech 2 147.00€ Pub
R9012HR-50g HyAgarose™ HR Agarose, PCR Grade, Green Agarose Product, 50g ACTGene 50g 148.00€
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1110-200 Caspase 4 Substrate LEVD pNA200 assays Biovision 200 assays 287.28€
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OP-0002-1  EpiQuik Nuclear Extraction Kit (100 assays) EpigenTek 239.40€ Pub
11952 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence* AAT Bioquest 1 kit 163.00€
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2052T Human monkey anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 518.81€
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4999-1000 Enhanced Green Fluorescent Protein (EGFP) Biovision 1 mg 1 895.00€ Pub
EAMPK-100 EnzyFluo™ AMPK Phosphorylation Assay Kit Bioassays 100 tests 373.00€ Pub
K124-25 Caspase 10 Fluorometric Assay Kit Biovision 25 assays 171.50€
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K716-100 Aconitase Activity Assay Kit Biovision 100 assays 357.00€ Pub
50CBR110 ImmunoComb Canine Antibody Test Kits: Canine Brucella AJMAL 120tests 685.00€
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1075-1000 Caspase-9 Substrate LEHD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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K251-100 Glutathione Fluorometric Detection Kit Biovision 100 assays 367.00€
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1023T Rat anti-porcine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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20334 Mouse anti-porcine type II collagen IgG3 antibody subtype assay kit, OPD Chondrex 400.05€
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3470-096-K Cultrex In Vitro Angiogenesis Assay Kit (***formerly HT CAS Tube Formation Kit***) Trevigen 96 Tests 298.00€ Pub
K110-25 Caspase 1 Fluorometric Assay Kit Biovision 25 assays 162.50€
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K609-100 Pyruvate Assay Kit Biovision 100 assays 425.00€ Pub
M1576 RubyGlowTM Luminescent Cytotoxicity Assay Kit, Contains a luciferase emitting red light, generates very stable luminescent signals. Allows multiplexing with currently available luciferase in cell-bas MarkerGene 187.00€
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1013-1000 Annexin V Cy5 Reagent Biovision 1000 assays 945.50€
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K155-100 Caspase 5 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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  P-4026-48  EpiQuik Global Acetyl Histone H4K16 Quantification Kit (Colorimetric) (48 assays) EpigenTek 358.00€
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1031 Human monkey anti-chick type I collagen IgG antibody assay kit, OPD Chondrex 528.36€
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2035 Mouse anti-human type II collagen IgG antibody assay kit, OPD Chondrex 434.70€
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36005 Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence* AAT Bioquest 10 plates 436.00€
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DIAP-250 QuantiChrom™ BCP Albumin Assay Kit Bioassays 250 299.00€ Pub
K111-400 Caspase 1 Colorimetric Assay Kit Biovision 400 assays 702.50€
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K618-100 Maltose and Glucose Assay Kit Biovision 100 assays 386.00€ Pub
M18825979 Clindamycin phosphate CAS Number [24729 96 2] Molekula 25 MG 151.00€
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1014-1000 Annexin V PE Reagent1000 assays Biovision 1000 assays 937.00€
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K160-100 Caspase 10 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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  P-4028-96  EpiQuik Global Acetyl Histone H4K12 Quantification Kit (Colorimetric) (96 assays) EpigenTek 606.00€
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PBD-20000 Phosphate Buffered Saline (10x) pH 7.4 Scy tek 20 L 366.00€
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AK8909-1000 BSA, Fatty Acid-Free(Assay Stabilizing) Akron 1kg 925.00€
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ETGA-200 EnzyChrom™ Triglyceride Assay Kit Bioassays 200 352.00€ Pub
K367-100 Coenzyme A (CoA) Assay Kit Biovision 100 assays 357.00€
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STA-330 OxiSelect™ TBARS Assay Kit (MDA Quantitation) Cell Biolabs 200 assays 489.00€ Pub
1117-200 Caspase-12 Substrate ATAD-AFC; Appearance Liquid Biovision 200 assays 416.75€
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P-1036-96 MethylFlash Hydroxymethylated DNA Quantification Kit Colorimetric EpigenTek 96 assays 862.59€
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22133 phosphate-regulating neutral endopeptidase antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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60311 PDE1B Assay Kit BPS Bioscience 96 reactions 491.00€
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ECRT-100 EnzyChrom™ Creatine Assay Kit Bioassays 100 360.00€ Pub
K145-100 Cathepsin H Activity Assay Kit Biovision 100 assays 357.00€
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K771-100 Trypsin Activity Assay Kit Biovision 100 assays 308.00€ Pub
P-3015-096 EpiQuik In Situ Histone H3K4 Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
1102-1000 Caspase 5 Substrate WEHD pNA1000 assays Biovision 1000 assays 579.29€
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K363-100 HDAC-3 Inhibitor Screening Kit Biovision 100 assays 503.00€
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11304 Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 163.00€
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