Product name :Caspase 2 Inhibitor Drug Screening Kit

Catalog Number :K152-100

Quantity :100 assays

Price :387.50 €

Pay now with :

Supplier :Biovision

 

Kit Summary: 
• Detection method- Fluorescence (Ex/Em 400/505) 
• Sample type- Cell samples treated with Caspase inhibitor drugs 
• Species reactivity- Mammalian 
• Kit size- 100 assays 
• Applications- Effective means for screening caspase inhibitors using fluorometric methods.

Features & Benefits: 
• Simple one-step procedure; takes only 1-1.5 hours 
• Fast and convenient 
• Inhibition of caspase-2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Kit components: 
• 2X Reaction Buffer 
• Caspase Substrate VDVAD-AFC (1 mM) 
• DTT (1 M) 
• Active Caspase-2 (Lyophilized) 
• Caspase Inhibitor, Z-VAD-FMK (2 mM)

Description: 
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-2 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Storage Conditions: 
-20°C

Shipping Conditions: 
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

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000028A PTEN ABM Goods 250ul 621.00€
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000049A U6 Empty (GFP tag) ABM Goods 250ul 621.00€
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000252A TGM2 ABM Goods 250ul 621.00€
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000445A Musculin ABM Goods 250ul 621.00€
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000630A CTSF S3 ABM Goods 250ul 621.00€
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1070-1000 Caspase 6 Substrate VEID pNA Biovision 1000 assays 613.31€
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1109-1000 Caspase 4 Substrate LEVD AFC Biovision 1000 assays 613.31€
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1977-500 Myeloperoxidase Inhibitor; Appearance White to off-white solid Biovision 500 mg 219.24€
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K112-200 Caspase 8 Fluorometric Assay Kit Biovision 200 assays 450.50€
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000121A MKK4(dn) ABM Goods 250ul 621.00€
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000338A STK25 ABM Goods 250ul 621.00€
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000696A Lin28 ABM Goods 250ul 473.00€
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1083-100 Active Human Caspase 3100 units Biovision 100 units 230.58€
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1127-20C EMAP II Inhibitor Z ASTD FMK 20 ul (10 mM) Biovision 20 ul (10 mM) 230.58€
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000071A NIK ABM Goods 250ul 621.00€
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1072-200 Caspase 2 Substrate VDVAD pNA200 assays Biovision 200 assays 287.28€
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K116-200 Caspase 2 Fluorometric Assay Kit Biovision 200 assays 468.50€
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000016A ZNF217 ABM Goods 250ul 621.00€
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000218A p21 ABM Goods 250ul 621.00€
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000416A ETS 1 ABM Goods 250ul 621.00€
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000603A MEF2 (luciferase) ABM Goods 250ul 621.00€
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1062-1000 Caspase-8 Substrate IETD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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1102-1000 Caspase-5 Substrate WEHD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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1921-5 Elastase Inhibitor, SPCK; Appearance White solid Biovision 5 mg 187.11€ Pub
228-10407-2 Eptifibatide Proteins Ray Biotech 25mg 292.00€
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1086-25 Active Human Caspase 625 units Biovision 25 units 183.33€
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K182-25 CaspGLOW Fluorescein Active Caspase 2 Staining Kit Biovision 25 assays 200.00€
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000485A TCF4 ABM Goods 250ul 621.00€
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22820 Cell Meter™ Caspase 3 7, 8 and 9 Activity Multiplexing Assay Kit *Tricolor Fluorescence* AAT Bioquest 100 Tests 280.00€
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