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#32252184   2020/03/31 To Up

Pioglitazone Alters the Proteomes of Normal Bladder Epithelial Cells but Shows No Tumorigenic Effects.

Pioglitazone, an antihyperglycemic drug, is widely used in diabetes mellitus patients with insulin resistance. Although pioglitazone is known to have a potential link to bladder cancer (BC), there have been contradictory results. This present study is designed to understand the regulatory mechanisms that drive the effects of pioglitazone on the bladder epithelial cells.
Muhammad Shahid, Minhyung Kim, Austin Yeon, Peng Jin, Woong-Ki Kim, Sungyong You, Jayoung Kim

1068 related Products with: Pioglitazone Alters the Proteomes of Normal Bladder Epithelial Cells but Shows No Tumorigenic Effects.



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#32252157   2020/03/31 To Up

Exogenous Stimulation of Human Intervertebral Disc Cells in 3-Dimensional Alginate Bead Culture With BMP2 and L51P: Cytocompatibility and Effects on Cell Phenotype.

Spinal fusion surgery is a common treatment modality for various pathologic conditions of the spine. The bone morphogenetic protein 2 (BMP2) analogue L51P acts as a general inhibitor of BMP antagonists, whereas it shows a weak affinity for BMP type I receptor. It is suggested that L51P applied in bone disorders might prevent side effects of highly concentrated BMP dosage applications in the order of milligrams. The objective of this study was to investigate the effects of L51P and BMP2 on intervertebral disc cells (IVDCs), i.e. on nucleus pulposus cells, on annulus fibrosus cells (AFCs), and on cartilaginous endplate cells (CEPCs), respectively, in 3-dimensional (3D) culture.
Rahel D May, Daniela A Frauchiger, Christoph E Albers, Willy Hofstetter, Benjamin Gantenbein

1777 related Products with: Exogenous Stimulation of Human Intervertebral Disc Cells in 3-Dimensional Alginate Bead Culture With BMP2 and L51P: Cytocompatibility and Effects on Cell Phenotype.

1.00 flask1 mg1.00 flask10 ug1.00 flask1.00 flask1100 extractions1.00 flask10 rxns

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#32252155   2020/03/31 To Up

Actions of Prostaglandins on Human Nucleus Pulposus Metabolism Inferred by Cyclooxygenase 2 Inhibition of Cytokine Activated Cells.

Low back pain is frequently treated with nonsteroidal anti-inflammatory drugs (NSAIDs), but little is known about intervertebral disc metabolism of the prostaglandins that are diminished by these drugs. Hence, this study aimed at delineating prostaglandin actions in cytokine activated disc cells by comparing the response of nucleus pulposus (NP) cells to the pro-inflammatory cytokine interleukin (IL)-1β with and without cyclooxygenase 2 (COX-2) inhibition.
Nam Vo, Brandon Couch, Joon Lee, Gwendolyn Sowa, James Kang, Studer Rebecca

1754 related Products with: Actions of Prostaglandins on Human Nucleus Pulposus Metabolism Inferred by Cyclooxygenase 2 Inhibition of Cytokine Activated Cells.

100 ug/vial10 ug1 mg1 mg100ug Lyophilized1.00 flask1mg100ug Lyophilized200 1.00 flask25mg16-22 Sample Kit

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#32252149   2020/03/31 To Up

Gene Therapy Approach for Intervertebral Disc Degeneration: An Update.

Intervertebral disc degeneration is the primary cause of back pain and associated with neurological disorders including radiculopathy, myelopathy, and paralysis. The currently available surgical treatments predominantly include the excision of pathological discs, resulting in the function loss, immobilization, and potential additional complications due to the altered biomechanics. Gene therapy approach involves gene transfer into cells, affects RNA and protein synthesis of the encoded genes in the recipient cells, and facilitates biological treatment. Relatively long-exerting therapeutic effects by gene therapy are potentially advantageous to treat slow progressive degenerative disc disease. In gene therapy, the delivery method and selection of target gene(s) are essential. Although gene therapy was first mediated by viral vectors, technological progress has enabled to apply nonviral vectors and polyplex micelles for the disc. While RNA interference successfully provides specific downregulation of multiple genes in the disc, clustered regularly interspaced short palindromic repeats (CRISPR) system has increased attention to alter the process of intervertebral disc degeneration. Then, more recent findings of our studies have suggested autophagy, the intracellular self-digestion, and recycling system under the negative regulation by the mammalian target of rapamycin (mTOR), as a gene therapy target in the disc. Here we briefly review backgrounds and applications of gene therapy for the disc, introducing strategies of autophagy and mTOR signaling modulation through selective RNA interference.
Yoshiki Takeoka, Takashi Yurube, Kotaro Nishida

1837 related Products with: Gene Therapy Approach for Intervertebral Disc Degeneration: An Update.

1.0 mg100μg100 µg 25 µg100ug Lyophilized100 µl100 100ug50 ug10ul100 TESTS50 assays

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#32252145   2020/03/23 To Up

Components of human breast milk: from macronutrient to microbiome and microRNA.

Human breast milk (HBM) is essential for the infant's growth and development right after birth and is an irreplaceable source of nutrition for early human survival. Various infant formulas have many similarities to HBM in many components, but there is no perfect substitute for HBM. Recently, various breast milk components and their roles have been studied according to the development of various analysis techniques. As is already well known, HBM contains about 87-88% water, and 124 g/L solid components as macronutrients, including about 7% (60-70 g/L) carbohydrates, 1% (8-10 g/L) protein, and 3.8% (35-40 g/L) fat. The composition may vary depending on the environmental factors, including maternal diet. Colostrum is low in fat but high in protein and relatively rich in immune-protective components. Although HBM contains enough vitamins to ensure normal growth of the infant, vitamins D and K may be insufficient, and the infant may require their supplementation. Growth factors in HBM also serve as various bioactive proteins and peptides on the intestinal tract, vasculature, nervous system, and endocrine system. In the past, HBM of a healthy mother was thought to be sterile. However, several subsequent studies have confirmed the presence of rich and diverse microbial communities in HBM. Some studies suggested that the genera Staphylococcus and Streptococcus may be universally predominant in HBM, but the origin of microbiota still remains controversial. Lastly, milk is the one of most abundant body fluid of microRNAs, which are known to play a role in various functions, such as immunoprotection and developmental programming, through delivering from HBM and absorption by intestinal epithelial cells. In conclusion, HBM is the most important source of nutrition for infants and includes microbiomes and miRNAs for growth, development, and immunity.
Su Yeong Kim, Dae Yong Yi

1234 related Products with: Components of human breast milk: from macronutrient to microbiome and microRNA.

100 ul50 ul1000 100 μg1 kit(96 Wells)One 96-Well Strip Micropl5One 96-Well Strip Micropl1 mgOne 96-Well Strip Micropl0.1 mg0.1 mg

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#32252114   2020/04/06 To Up

Combined Use of CytoSorb and ECMO in Patients with Severe Pneumogenic Sepsis.

 High morbidity and mortality are frequently reported in intensive care patients suffering from severe sepsis with systemic inflammation. With the development of severe respiratory failure, extracorporeal membrane oxygenation (ECMO) is often required. In this study, cytokine adsorption therapy in combination with ECMO is applied in patients with acute respiratory distress syndrome (ARDS) due to severe pneumogenic sepsis. The efficacy of this therapy is evaluated compared with a historical cohort without hemoadsorption therapy.
Ali Akil, Stephan Ziegeler, Jan Reichelt, Stephanie Rehers, Omer Abdalla, Michael Semik, Stefan Fischer

1993 related Products with: Combined Use of CytoSorb and ECMO in Patients with Severe Pneumogenic Sepsis.

100ug100 μg1 Set100 μg1 mg1 Set100 μg100 mg1 Set

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#32252105   2020/04/06 To Up

COMP-Ang-1 Improves Glucose Uptake in db/db Mice with Type 2 Diabetes.

Cartilage oligomeric matrix protein (COMP)-Angiopoietin-1 is a potent angiopoietin-1 (Ang-1) variant that possesses therapeutic potential in angiogenesis and vascular endothelial dysfunction. Noteworthy, we have shown that COMP-Ang-1 improves hyperglycemia and neuroregeneration in / mice. However, the mechanism of the antidiabetic effect of COMP-Ang-1 is completely unknown. Therefore, we elucidated the diabetes protective molecular mechanisms of COMP-Ang-1 in diabetic / mouse model. COMP-Ang-1 (0.5 ng/g body weight) or aqueous NaCl solution was injected intraperitoneally per day in 21 consecutive days into 3-month old, male / mice (n=10 per group). Blood glucose and HbA1c levels were determined at baseline and 21 days after COMP-Ang-1 or NaCl treatment. The effect of COMP-Ang-1 on glucose uptake was investigated by euglycemic-hyperinsulinemic clamp studies and key genes of glucose metabolism were studied by Western blot analysis. Our findings indicate that COMP-Ang-1 improves glucose metabolism in a tissue specific manner by regulating HIF-1α transcriptional genes of GLUT-1 expression.
Petra Baum, Sabine Paeschke, Nora Klöting, Matthias Blüher, Matthias Kern, Heike Serke, Marcin Nowicki, Joanna Kosacka

1923 related Products with: COMP-Ang-1 Improves Glucose Uptake in db/db Mice with Type 2 Diabetes.

10mg200ul100ug Lyophilized100ug Lyophilized200ul5/100 Packing /sleeve/bo100ug Lyophilized20000 Units10mg200ul

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#32252102   2020/04/06 To Up

Is Exercise a Match for Cold Exposure? Common Molecular Framework for Adipose Tissue Browning.

Exercise is commonly utilized for weight loss, yet research has focused less on specific modifications to adipose tissue metabolism. White adipose tissue (WAT) is the storage form of fat, whereas brown adipose tissue (BAT) is a thermogenic tissue whose uncoupling increases energy expenditure. The most established BAT activator is cold exposure, which also transforms WAT into "beige cells" that express uncoupling protein 1 (UCP1). Preliminary evidence in rodents suggests exercise elicits similar effects. The purpose of this review is to parallel and examine differences between exercise and cold exposure on BAT activation and beige induction. Like cold exposure, exercise stimulates the sympathetic nervous system and activates molecular pathways responsible for BAT/beige activation, including upregulation of BAT activation markers (UCP1, proliferator-activated receptor-gamma coactivator-1α) and stimulation of endocrine activators (fibroblast growth factor-21, irisin, and natriuretic peptides). Further, certain BAT activators are altered exclusively by exercise (interleukin-6, lactate). Markers of BAT activation increase from both cold exposure and exercise, whereas effects in WAT are compartment-specific. Stimulation of endocrine activators depends on numerous factors, including stimulus intensity and duration. Evidence of these analogous, albeit not mirrored, mechanisms is demonstrated by increases in adipose activity in rodents, while effects remain challenging to quantify in humans.
Alexandra R Martin, Soonkyu Chung, Karsten Koehler

2955 related Products with: Is Exercise a Match for Cold Exposure? Common Molecular Framework for Adipose Tissue Browning.

0.1ml (1mg/ml)0.1 ml25 µg0.1 mg100 TESTS0.2 mg0.1 mg250 ml20 ug25 µg

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#32252096   2020/04/06 To Up

Reconstruction Using Free Flaps for Diabetic Heel Defects: Outcomes and Risk Factor Analysis.

 Overall success rate after diabetic foot reconstruction using free flap has been acceptable, but certain parts of the foot like the heel remains more challenging.
Hyung Bae Kim, Mehmet Altiparmak, Changsik John Pak, Hyunsuk Peter Suh, Joon Pio Hong

1084 related Products with: Reconstruction Using Free Flaps for Diabetic Heel Defects: Outcomes and Risk Factor Analysis.

96 wells (1 kit)20 ug5L100μg1 kit(96 Wells)1 module25 mg2ug100ug Lyophilized100 units96 wells (1 kit)100 ug

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#32252065   2020/04/06 To Up

Vocal cord palsy in refractory anti-melanoma differentiation-associated protein 5 antibody-positive dermatomyositis.


Omar Suhail Alsaed, Laith Ishaq Alamlih, Khalifa Abdulsalam Farfar, Tasleem Raza N Mohd, Samar A Al Razaq Alemadi, Hani Ali Malallah

2920 related Products with: Vocal cord palsy in refractory anti-melanoma differentiation-associated protein 5 antibody-positive dermatomyositis.

100ug Lyophilized100ug Lyophilized100 μg100100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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