Only in Titles

Search results for: replacing

paperclip

#33631105   2021/02/17 To Up

A unique histone 3 lysine 14 chromatin signature underlies tissue-specific gene regulation.

Organismal development and cell differentiation critically depend on chromatin state transitions. However, certain developmentally regulated genes lack histone 3 lysine 9 and 27 acetylation (H3K9ac and H3K27ac, respectively) and histone 3 lysine 4 (H3K4) methylation, histone modifications common to most active genes. Here we describe a chromatin state featuring unique histone 3 lysine 14 acetylation (H3K14ac) peaks in key tissue-specific genes in Drosophila and human cells. Replacing H3K14 in Drosophila demonstrates that H3K14 is essential for expression of genes devoid of canonical histone modifications in the embryonic gut and larval wing imaginal disc, causing lethality and defective wing patterning. We find that the SWI/SNF protein Brahma (Brm) recognizes H3K14ac, that brm acts in the same genetic pathway as H3K14R, and that chromatin accessibility at H3K14ac-unique genes is decreased in H3K14R mutants. Our results show that acetylation of a single lysine is essential at genes devoid of canonical histone marks and uncover an important requirement for H3K14 in tissue-specific gene regulation.
Isabel Regadas, Olle Dahlberg, Roshan Vaid, Oanh Ho, Sergey Belikov, Gunjan Dixit, Sebastian Deindl, Jiayu Wen, Mattias Mannervik

2386 related Products with: A unique histone 3 lysine 14 chromatin signature underlies tissue-specific gene regulation.

2 Pieces/Box50 ug50 ug100 ul50 ug50 ug50 ug50 ug

Related Pathways

paperclip

#33630745   2021/01/16 To Up

App Usage and Usability Impressions of a Barcode-Based Digital Contact Tracing Platform for COVID-19: Survey Study.

The novel coronavirus (COVID-19) has drastically changed life in the United States, as the country has recorded over 23 million cases and 383,000 deaths to date. In the lead-up to widespread vaccine deployment, testing and surveillance are critical for detecting and stopping possible routes of transmission. Contact tracing has become an important surveillance measure for COVID-19 control in the United States, and mobile health interventions have found increased prominence in this space.
Thomas Foster Scherr, Jenna DeSousa, Carson Moore, Austin Hardcastle, David Wilson Wright

2849 related Products with: App Usage and Usability Impressions of a Barcode-Based Digital Contact Tracing Platform for COVID-19: Survey Study.

0.1 ml 100ul 100ul 100ul250 ml 100ul0.2 mg25 µg1 LITRE100 TESTS100 ml

Related Pathways

paperclip

Error loading info... Pleas try again later.
paperclip

#33628860   2021/02/19 To Up

Isotonic ion replacement can lower the threshold for selective infrared neural inhibition.

Infrared (IR) inhibition can selectively block peripheral sensory nerve fibers, a potential treatment for autonomic-dysfunction-related diseases (e.g., neuropathic pain and interstitial cystitis). Lowering the IR inhibition threshold can increase its translational potentials. Infrared induces inhibition by enhancing potassium channel activation. We hypothesized that the IR dose threshold could be reduced by combining it with isotonic ion replacement. We tested the IR inhibition threshold on the pleural-abdominal connective of . Using a customized chamber system, the IR inhibition was applied either in normal saline or in isotonic ion-replaced saline, which could be high glucose saline, high choline saline, or high glucose/high choline saline. Each modified saline was at a subthreshold concentration for inhibiting neural conduction. We showed that isotonically replacing ions in saline with glucose and/or choline can reduce the IR threshold and temperature threshold of neural inhibition. Furthermore, the size selectivity of IR inhibition was preserved when combined with high glucose/high choline saline. The present work of IR inhibition combined with isotonic ion replacement will guide further development of a more effective size-selective IR inhibition modality for future research and translational applications.
Junqi Zhuo, Zihui Ou, Yuhan Zhang, Elizabeth M Jackson, Sachin S Shankar, Matthew T McPheeters, Jeremy B Ford, E Duco Jansen, Hillel J Chiel, Michael W Jenkins

2924 related Products with: Isotonic ion replacement can lower the threshold for selective infrared neural inhibition.

0.25 mg1025ml5 mg

Related Pathways

paperclip

#33627870   2021/02/24 To Up

An organoid-based organ-repurposing approach to treat short bowel syndrome.

The small intestine is the main organ for nutrient absorption, and its extensive resection leads to malabsorption and wasting conditions referred to as short bowel syndrome (SBS). Organoid technology enables an efficient expansion of intestinal epithelium tissue in vitro, but reconstruction of the whole small intestine, including the complex lymphovascular system, has remained challenging. Here we generate a functional small intestinalized colon (SIC) by replacing the native colonic epithelium with ileum-derived organoids. We first find that xenotransplanted human ileum organoids maintain their regional identity and form nascent villus structures in the mouse colon. In vitro culture of an organoid monolayer further reveals an essential role for luminal mechanistic flow in the formation of villi. We then develop a rat SIC model by repositioning the SIC at the ileocaecal junction, where the epithelium is exposed to a constant luminal stream of intestinal juice. This anatomical relocation provides the SIC with organ structures of the small intestine, including intact vasculature and innervation, villous structures, and the lacteal (a fat-absorbing lymphatic structure specific to the small intestine). The SIC has absorptive functions and markedly ameliorates intestinal failure in a rat model of SBS, whereas transplantation of colon organoids instead of ileum organoids invariably leads to mortality. These data provide a proof of principle for the use of intestinal organoids for regenerative purposes, and offer a feasible strategy for SBS treatment.
Shinya Sugimoto, Eiji Kobayashi, Masayuki Fujii, Yuki Ohta, Kazuya Arai, Mami Matano, Keiko Ishikawa, Kentaro Miyamoto, Kohta Toshimitsu, Sirirat Takahashi, Kosaku Nanki, Yoji Hakamata, Takanori Kanai, Toshiro Sato

2834 related Products with: An organoid-based organ-repurposing approach to treat short bowel syndrome.

1 mL1 mg0.1 mg1 module100 1 ml

Related Pathways

paperclip

#33627720   2021/02/24 To Up

Machine learning approaches for the prediction of bone mineral density by using genomic and phenotypic data of 5130 older men.

The study aimed to utilize machine learning (ML) approaches and genomic data to develop a prediction model for bone mineral density (BMD) and identify the best modeling approach for BMD prediction. The genomic and phenotypic data of Osteoporotic Fractures in Men Study (n = 5130) was analyzed. Genetic risk score (GRS) was calculated from 1103 associated SNPs for each participant after a comprehensive genotype imputation. Data were normalized and divided into a training set (80%) and a validation set (20%) for analysis. Random forest, gradient boosting, neural network, and linear regression were used to develop BMD prediction models separately. Ten-fold cross-validation was used for hyper-parameters optimization. Mean square error and mean absolute error were used to assess model performance. When using GRS and phenotypic covariates as the predictors, all ML models' performance and linear regression in BMD prediction were similar. However, when replacing GRS with the 1103 individual SNPs in the model, ML models performed significantly better than linear regression (with lasso regularization), and the gradient boosting model performed the best. Our study suggested that ML models, especially gradient boosting, can improve BMD prediction in genomic data.
Qing Wu, Fatma Nasoz, Jongyun Jung, Bibek Bhattarai, Mira V Han, Robert A Greenes, Kenneth G Saag

2042 related Products with: Machine learning approaches for the prediction of bone mineral density by using genomic and phenotypic data of 5130 older men.

10ìg100μg 5 G

Related Pathways

paperclip

#33625792   2021/02/24 To Up

Development of an inverse-low-temperature plasma ionization source for LC-MS.

An argon inverse low-temperature plasma (iLTP) ionization source for liquid chromatography- tandem mass spectrometry was developed. The iLTP is constructed by simple chromatographic supply materials and is implemented into an APCI ion source replacing the APCI discharge needle electrode. The newly developed ion source was coupled to an ultra-high performance liquid chromatograph (UHPLC).
Dominik Brecht, Florian Uteschil, Oliver J Schmitz

1217 related Products with: Development of an inverse-low-temperature plasma ionization source for LC-MS.

100ul 100ul 100ul100ug100ug 100ul 100ul 100ul 100ul 100ul 100ul50ul

Related Pathways

    No related Items
paperclip

#33624196   2021/02/23 To Up

Influence of halogen atom substitution and neutral HCN/anion CN Lewis base on the triel-bonding interactions.

Triel-bonding interactions composed of Lewis acid TrOHH/TrOHX/TrOHX (Tr = B, Al, Ga; X = F, Cl, Br) molecule and Lewis base neutral HCN or anionic CN molecule are of research significance in bond properties, which has been investigated at MP2/aug-cc-pVTZ theory level. It is also feasible to study the halogen atom substituent effect and influence of different Lewis bases on the formation of triel bond. AIM analyses reveal that Tr (Tr = B, Al, Ga)···N bond critical point (BCP) exists in all studied triel bond. In the formation of triel bonding, compared with Lewis base HCN molecule, Lewis base anionic CN can participate in a stronger triel bond. Specifically, the structural change, deformation energy, and charge transfer of CN complexes are all larger than that of HCN complexes. In addition, halogen atom substitution effect is also discussed. MEP value and binding energy of HCN and CN complexes all increase after replacing one or two hydrogen atoms by halogen atoms (F, Cl, Br) in Lewis acid. Especially, replacing two hydrogen atoms by halogen atoms in Lewis acid has more remarkable enhancement in MEP value and binding energy than that of replacing only one hydrogen atom. After replacement, binding energy can be increased by 21.77 kcal/mol. The neutral and anionic triel-bonded complexes composed by Lewis acid TrOHH/TrOHX/TrOHX (Tr = B, Al, Ga; X = F, Cl, Br) with Lewis base HCN and CN are systematically investigated at MP2/aug-cc-pVTZ level. The neutral (HCN) triel bonding is weaker than the anionic (CN) triel bonding due to the smaller MEP value of the neutral HCN molecule. The replacement of hydrogens (-H) in Lewis acid by electron-withdrawing groups (-F, -Cl, -Br) has a prominent enhancement effect on the MEP value of π-hole and triel-bonding strength.
Yuchun Li, Xiaoting Wang, Hui Wang, Yuxiang Ni, Hongyan Wang

1435 related Products with: Influence of halogen atom substitution and neutral HCN/anion CN Lewis base on the triel-bonding interactions.

100 100 mg100ug100preparations100ug Lyophilized1 6 ml Ready-to-use 2 ml Ready-to-use 25 MG0.1 mg100ul100ug Lyophilized

Related Pathways

paperclip

#33622994   2021/02/24 To Up

Construction and Analysis of Food-grade β-galactosidase Overexpression System.

contains two types of β-galactosidase, LacLM and LacZ, belonging to different glycoside hydrolase families. The difference in function between them has been unclear so far for practical application. In this study, and from ATCC51647 were cloned into constitutive lactobacillal expression vector pMG36e, respectively. Furtherly, pMG36n- was constructed from pMG36e- by replacing erythromycin with nisin as selective marker for food-grade expressing systems in WCFS1, designated recombinant LacLM and LacZ respectively. The results from hydrolysis of o-nitrophenyl-β-galactopyranoside (ONPG) showed that the β-galactosidases activity of the recombinant LacLM and LacZ was 1460% and 670% higher than that of the original . Moreover, the lactose hydrolytic activity of recombinant LacLM was higher than that of LacZ in milk. Nevertheless, compare to LacZ, in 25% lactose solution the galacto-oligosaccharides (GOS) production of recombinant LacLM was lower. Therefore, two β-galactopyranosides could play different roles in carbohydrate metabolism of . In addition, maximal growths rate of two recombinant strains were evaluated with different temperature level and nisin concentration in fermentations assay for practical purpose. The results displayed that 37 °C and 20-40 U/ mL nisin were the optimal fermentation conditions for the growth of recombinant β-galactosidase strains. Altogether the food-grade Expression system of recombinant β-galactosidase was feasible for applications in the food and dairy industry.
Xi He, MingJian Luan, Ning Han, Ting Wang, Xiangzhong Zhao, Yanyan Yao

1290 related Products with: Construction and Analysis of Food-grade β-galactosidase Overexpression System.

1

Related Pathways

paperclip

#33622552   2021/02/20 To Up

Performance of fixed dental prostheses up to 6 years-A massive data analysis.

Metal and metal-ceramic fixed dental prostheses (FDPs) have been clinically determined to provide long-term durable restorations. However, data on their performance under practice conditions are sparse.
Michael Raedel, Heinz-Werner Priess, Steffen Bohm, Michael H Walter

2068 related Products with: Performance of fixed dental prostheses up to 6 years-A massive data analysis.

1 module1 module1 module1 module1 module1 module1 module1 module1 module1 module1 module1 module

Related Pathways