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#35763565   2022/06/28 To Up

A vascularized model of the human liver mimics regenerative responses.

Liver regeneration is a well-orchestrated process that is typically studied in animal models. Although previous animal studies have offered many insights into liver regeneration, human biology is less well understood. To this end, we developed a three-dimensional (3D) platform called structurally vascularized hepatic ensembles for analyzing regeneration (SHEAR) to model multiple aspects of human liver regeneration. SHEAR enables control over hemodynamic alterations to mimic those that occur during liver injury and regeneration and supports the administration of biochemical inputs such as cytokines and paracrine interactions with endothelial cells. We found that exposing the endothelium-lined channel to fluid flow led to increased secretion of regeneration-associated factors. Stimulation with relevant cytokines not only amplified the secretory response, but also induced cell-cycle entry of primary human hepatocytes (PHHs) embedded within the device. Further, we identified endothelial-derived mediators that are sufficient to initiate proliferation of PHHs in this context. Collectively, the data presented here underscore the importance of multicellular models that can recapitulate high-level tissue functions and demonstrate that the SHEAR device can be used to discover and validate conditions that promote human liver regeneration.
Arnav Chhabra, H-H Greco Song, Katarzyna A Grzelak, William J Polacheck, Heather E Fleming, Christopher S Chen, Sangeeta N Bhatia

2420 related Products with: A vascularized model of the human liver mimics regenerative responses.

1 mg 100ul 100ul0.1 mg 100ul1 mg100μl100 μg0.5 ml0.7 mg100.00 ug20

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#35763058   2022/06/28 To Up

Brain Cell Senescence: A New Therapeutic Target for the Acute Treatment of Ischemic Stroke.

Aging is a major risk factor for cerebral infarction. Since cellular senescence is intrinsic to aging, we postulated that stroke-induced cellular senescence might contribute to neural dysfunction. Adult male Wistar rats underwent 60-minute middle cerebral artery occlusion and were grouped according to 3 reperfusion times: 24 hours, 3, and 7 days. The major biomarkers of senescence: 1) accumulation of the lysosomal pigment, lipofuscin; 2) expression of the cell cycle arrest markers p21, p53, and p16INK4a; and 3) expression of the senescence-associated secretory phenotype cytokines interleukin-6 (IL-6), tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) were investigated in brain samples. Lipofuscin accumulation was scarce at the initial stage of brain damage (24 hours), but progressively increased until it reached massive distribution at 7 days post-ischemia. Lipofuscin granules (aggresomes) were mainly confined to the infarcted areas, that is parietal cortex and adjacent caudate-putamen, which were equally affected. The expression of p21, p53, and p16INK4a, and that of IL-6, TNF-α, and IL-1β, was significantly higher in the ischemic hemisphere than in the non-ischemic hemisphere. These data indicate that brain cell senescence develops during acute ischemic infarction and suggest that the acute treatment of ischemic stroke might be enhanced using senolytic drugs.
Júlia Baixauli-Martín, Alicia Aliena-Valero, María Castelló-Ruiz, María C Burguete, Mikahela A López-Morales, Daniel Muñoz-Espín, Germán Torregrosa, Juan B Salom

1788 related Products with: Brain Cell Senescence: A New Therapeutic Target for the Acute Treatment of Ischemic Stroke.

25 µg0.1 mg0.1ml (1mg/ml)0.2 mg0.1ml (1mg/ml)100 TESTS

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#35762614   2022/06/28 To Up

Secretory phospholipase A2 expression and activity in preterm clinical chorioamnionitis with fetal involvement.

Secretory phospholipase A2 (sPLA2) regulates the first step of inflammatory cascade and is involved in several pathological processes. sPLA2 also plays a role in preterm labor and parturition, since they are triggered by inflammatory mediators such as prostaglandins. Interestingly, chorioamnionitis (i.e.: the presence of intrauterine inflammation) is also often associated with preterm birth. We aimed to verify if chorioamnionitis with fetal involvement modifies sPLA2 activity and expression profile in mothers and neonates delivered prematurely. We collected maternal plasma and amniotic fluid, as well as bronchoalveolar lavage fluid from preterm neonates born to mothers with or without clinical chorioamnionitis with fetal involvement. We measured concentrations of sPLA2 subtype-IIA and -IB, total enzyme activity and proteins. Urea ratio was used to obtain epithelial lining fluid concentrations. Enzyme activity measured in maternal plasma (p<0.001) and amniotic fluid (p<0.001) was higher in chorioamnionitis cases than in controls. This was mainly due to the increased production of sPLA2-IIA as the subtype -IB was present in a smaller amount and was similar between the two groups; sPLA2-IIA was increased in epithelial lining fluid (p=0.045) or increased, although without statistical significance, in maternal plasma (p=0.06) and amniotic fluid (p=0.08) of chorioamnionitis cases. Cytokines known to increase sPLA2-IIA expression (TNF-a, IL-1b) or whose expression was increased by sPLA2-IIA (IL-8) were higher in histologically confirmed chorioamnionitis (TNF-a (p=0.028), IL-1b (p<0.001) and IL-8 (p=0.038)). These data represent the basis for future studies on sPLA2-IIA inhibition to prevent deleterious consequences of chorioamnionitis and preterm birth.
Daniele De Luca, Silvia Foligno, Chiara Autilio, Alexandre Vivanti, Melanie Vanderkerckhove, Jelena Martinovic, Roberto Raschetti, Loïc Guillot, Lhousseine Touqui

1064 related Products with: Secretory phospholipase A2 expression and activity in preterm clinical chorioamnionitis with fetal involvement.

400Tests

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#35762404   2022/06/28 To Up

Digital histological morphometry of the human pineal gland in a postmortem study, with endocrine and neurological clinical implications.

The pineal gland is a small-sized, photo neuroendocrine organ in the midline of the brain that synthesises and secretes melatonin and serotonin. Chords and islands of pinealocytes constitute the secretory parenchyma, while glial tissue and calcifications represent degenerative changes. This study examined human postmortem pineal glands to microscopically assess morphological changes possibly associated with clinical data, by using digital techniques. A retrospective autopsy study has been performed on 72 paediatric and adult autopsy cases. The glands have been processed for histological analysis and immunohistochemical staining with synaptophysin (SYN), neuron-specific enolase (NSE), and neurofilament (NF). Slides were digitally scanned. Morphometric data were obtained using CaseViewer and ImageJ. The comorbidities used for correlation with morphometric data were obesity, type 2 diabetes, adrenal gland adenoma, goitre, chronic pancreatitis, arterial hypertension, and mixed dementia. Thirty-three females and 39 males were included in the study. Increased secretory parenchyma was found in patients with chronic pancreatitis, arterial hypertension, and adrenal gland adenoma. Reduced activity was found in patients with type 2 diabetes, obesity, advanced pineal calcification, mixed dementia, and old age. There were no changes associated with goitre, cachexia, or Willis's polygon atherosclerosis. No significant differences between gender were found. The activity of the pineal gland can be assessed by quantitative immunohistochemistry of neuroendocrine and structural pinealocyte markers and observation of glial tissue and calcifications. There is a need for further research to evaluate the clinical impact of these morphological changes on the neuroendocrine systems, with clinical implications in endocrinology, neurology, and even psychiatry. Digital techniques offer a more exact analysis of histological data.
Bogdan-Alexandru Gheban, Horațiu Alexandru Colosi, Ioana-Andreea Gheban-Roșca, Carmen Georgiu, Dan Gheban, Doinița Crișan, Maria Crișan

2561 related Products with: Digital histological morphometry of the human pineal gland in a postmortem study, with endocrine and neurological clinical implications.

100 μg100 μg

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#35761913   2022/05/25 To Up

Anaesthetic Management of Secretory Paraganglioma With Cyanotic Heart Disease: Double Trouble.

Paragangliomas (PGL) in paediatric patients are rarely diagnosed with neuroendocrine tumours. Anaesthetic management of paraganglioma is challenging due to exaggerated haemodynamic alterations. Further associated cardiovascular diseases like congenital cyanotic heart disease (CCHD) with paragangliomas make its management daring and if not properly managed can increase morbidity. We herein discuss the successful anaesthetic management of a 10-year-old child with paraganglioma and associated atrial septal defect (ASD) with pulmonary stenosis (PS) for adrenalectomy. Overlapping the clinical spectrum between CCHD and catecholamine-secreting tumour makes the case very challenging. Management of the patient with CCHD and PGL needs a multidisciplinary approach, and intensive vigilance and monitoring are needed for the successful management of such challenging cases.
Ruma Thakuria, Manpreet Kaur, Rushil Vladimir, Rajeshwari Subramaniam

1211 related Products with: Anaesthetic Management of Secretory Paraganglioma With Cyanotic Heart Disease: Double Trouble.

100ug Lyophilized5000.25 mg100 1ml

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#35761891   2022/06/20 To Up

Antihyperglycemic and antihyperlipidemic activities of hydroethanolic extract of the fruit of in streptozotocin-induced diabetic rats.

This work evaluated the antihyperglycemic and antihyperlipidemic activities of pulp extracts of fruits in rats.
Takuissu Nguemto Guy Roussel, Fonkoua Martin, Youovop Fotso Janvier Aimé, Edoun Ebouel Ferdinand Lanvin, Dibacto Kemadjou Ruth Edwige, Azantsa Kingue Boris, Ngondi Judith Laure, Oben Julius Enyong

2289 related Products with: Antihyperglycemic and antihyperlipidemic activities of hydroethanolic extract of the fruit of in streptozotocin-induced diabetic rats.

1 mg1 mg 5 G400 ug400 ug50 ul 100ul2ug5ug100 ul

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#35761342   2022/06/27 To Up

Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis by promoter engineering and translation initiation efficiency optimization.

A raw starch-degrading α-amylase from Pontibacillus sp. ZY (AmyZ1), previously screened by our laboratory, showed a promising application potential for starch-processing industries. However, the AmyZ1 secretory production still under investigation, which seriously restricts its application in the starch-processing industry. On the other hand, Bacillus subtilis is widely used to achieve the extracellular expression of target proteins.
He Li, Dongbang Yao, Yan Pan, Xin Chen, Zemin Fang, Yazhong Xiao

1288 related Products with: Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis by promoter engineering and translation initiation efficiency optimization.

96 tests96 tests250ul5 mg96 tests96 tests1 Set1 Set

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#35761329   2022/06/27 To Up

SHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli.

In previous work, we developed an E. coli extracellular secretion platform XTHHly based on the hemolysin A secretion system. It can produce bioactive peptides with simple purification procedures. However, the wider application of this platform is limited by poor secretion efficiency.
Wen Zhu, Yang Wang, Liangyin Lv, Hui Wang, Wenqiang Shi, Zexin Liu, Wei Yang, Jianwei Zhu, Huili Lu

2255 related Products with: SHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli.

25mg100ul200 200 200 2 Pieces/Box100 units200 50

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#35761183   2022/06/27 To Up

Comparative genomics analysis and virulence-related factors in novel Aliarcobacter faecis and Aliarcobacter lanthieri species identified as potential opportunistic pathogens.

Emerging pathogenic bacteria are an increasing threat to public health. Two recently described species of the genus Aliarcobacter, A. faecis and A. lanthieri, isolated from human or livestock feces, are closely related to Aliarcobacter zoonotic pathogens (A. cryaerophilus, A. skirrowii, and A. butzleri). In this study, comparative genomics analysis was carried out to examine the virulence-related, including virulence, antibiotic, and toxin (VAT) factors in the reference strains of A. faecis and A. lanthieri that may enable them to become potentially opportunistic zoonotic pathogens.
Jiacheng Chuan, Anatoly Belov, Michel Cloutier, Xiang Li, Izhar U H Khan, Wen Chen

2760 related Products with: Comparative genomics analysis and virulence-related factors in novel Aliarcobacter faecis and Aliarcobacter lanthieri species identified as potential opportunistic pathogens.

96T50 ug 50 ug 50 ug 1000 100 assays100ug1 kit1 mg24 tests96

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#35760912   2022/06/27 To Up

The diversity of elaborate petals in Isopyreae (Ranunculaceae): a special focus on nectary structure.

Elaborate petals are highly diverse in morphology, structure, and epidermal differentiation and play a key role in attracting pollinators. There have been few studies on the elaborate structure of petals in the tribe Isopyreae (Ranunculaceae). Seven genera in Isopyreae (Aquilegia, Semiaquilegia, Urophysa, Isopyrum, Paraquilegia, Dichocarpum, and Leptopyrum) have petals that vary in morphology, and two genera (Enemion and Thalictrum) have no petals. The petals of nine species belonged to 7 genera in the tribe were studied to reveal their nectary structure, epidermal micromorphology and ancestral traits. The petal nectaries of Isopyreae examined in this study were located at the tip of spurs (Aquilegia yabeana and A. rockii), or the bottom of shallow sacs (Semiaquilegia adoxoides, Urophysa henryi, Isopyrum manshuricum, and Paraquilegia microphylla), a cup-shaped structure (Dichocarpum fargesii) and a bilabiate structure (Leptopyrum fumarioides). The petal nectary of eight species in Isopyreae (except A. ecalcarata) was composed of secretory epidermis, nectary parenchyma, and vascular tissues, and some sieve tubes reached the secretory parenchyma cells. Among the eight species with nectaries examined in the present study, A. yabeana had the most developed nectaries, with 10-15 layers of secretory parenchyma cells. The epidermal cells of mature petals of the nine species were divided into 11 types. Among these 11 types, there were two types of secretory cells and two types of trichomes. Aquilegia yabeana and A. rockii had the highest number of cell types (eight types), and I. manshuricum and L. fumarioides had the lowest number of cell types (three types). Aquilegia ecalcarata had no secretory cells, and the papillose conical polygonal secretory cells of D. fargesii were different from those of the other seven species with nectaries. Trichomes were found only in Aquilegia, Semiaquilegia, Urophysa, and Paraquilegia. The ancestral mode of nectar presentation in Isopyreae was petals with hidden nectar (70.58%). The different modes of nectar presentation in petals may reflect adaptations to different pollinators in Isopyreae.
Qing-Qing Zhu, Cheng Xue, Li Sun, Xin Zhong, Xin-Xin Zhu, Yi Ren, Xiao-Hui Zhang

2129 related Products with: The diversity of elaborate petals in Isopyreae (Ranunculaceae): a special focus on nectary structure.

1100ug100.00 ug100ug

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