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#33080653   2020/10/20 To Up

[Changes in claw and leg health and hair cortisol concentration in dairy cows during the transition from tie-stall to free-stall housing - A study of a medium-sized farm].

Claw health and carpal and tarsal lesions were assessed in a dairy herd during the transition from a tie-stall to a free-stall barn. The stress associated with the change in housing was also evaluated.
Karl Nuss, Simon Nogler, Isabelle Lüchinger, Michael Hässig, Laura Pieper, Gabriela Knubben-Schweizer

1423 related Products with: [Changes in claw and leg health and hair cortisol concentration in dairy cows during the transition from tie-stall to free-stall housing - A study of a medium-sized farm].

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#33080565   2020/10/13 To Up

3D finite element models from serial section histology of skeletal muscle tissue - The role of micro-architecture on mechanical behaviour.

In this contribution we create three-dimensional (3D) finite element models from a series of histological sections of porcine skeletal muscle tissue. Image registration is performed on the stained sections by affinely aligning them using auxiliary markers, followed by image segmentation to determine muscle fibres and the extracellular matrix in each section, with particular regard to the continuity of the fibres through the stack. With this information, 3D virtual tissue samples are reconstructed, discretised, and associated with appropriate non-linear elastic anisotropic material models. While the gross anatomy is directly obtained from the images, the local directions of anisotropy were determined by the use of an analogy with steady state diffusion. The influence of the number of histological sections considered for reconstruction on the numerically simulated mechanical response of the virtual tissue samples is then studied. The results show that muscle tissue is fairly heterogeneous along the fascicles, and that transverse isotropy is inadequate in describing their material symmetry at the typical length scale of a fascicle. Numerical simulations of different load cases suggest that ignoring the undulations of fibres and their non-uniform cross-sections only moderately affects the passive response of the tissue in tensile and compressive modes, but can become crucial when predicting the response to generic loads and activation.
R Kuravi, K Leichsenring, M Böl, A E Ehret

2695 related Products with: 3D finite element models from serial section histology of skeletal muscle tissue - The role of micro-architecture on mechanical behaviour.



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#33080312   2020/10/17 To Up

Cathepsins L and B in Dysdercus peruvianus, Rhodnius prolixus, and Mahanarva fimbriolata. Looking for enzyme adaptations to digestion.

Cysteine peptidases (CP) play a role as digestive enzymes in hemipterans similar to serine peptidases in most other insects. There are two major CPs: cathepsin L (CAL), which is an endopeptidase and cathepsin B (CAB) that is both an exopeptidase and a minor endopeptidase. There are thirteen putative CALs in Dysdercus peruvianus, which in some cases were confirmed by cloning their encoding genes. RNA-seq data showed that DpCAL5 is mainly expressed in the anterior midgut (AM), DpCAL10 in carcass (whole body less midgut), suggesting it is a lysosomal enzyme, and the other DpCALs are expressed in middle (MM) and posterior (PM) midgut. The expression data were confirmed by qPCR and enzyme secretion to midgut lumen by a proteomic approach. Two CAL activities were isolated by chromatography from midgut samples with similar kinetic properties toward small substrates. Docking analysis of a long peptide with several DpCALs modeled with digestive Tenebrio molitor CAL (TmCAL3) as template showed that on adapting to luminal digestion DpCALs (chiefly DpCAL5) changed in relation to their ancestral lysosomal enzyme (DpCAL10) mainly at its S2 subsite. A similar conclusion arrived from structure alignment-based clustering of DpCALs based on structural similarity of the modeled structures. Changes mostly on S2 subsite could mean the enzymes turn out less peptide-bond selective, as described in TmCALs. R. prolixus CALs changed on adapting to luminal digestion, although less than DpCALs. Both D. peruvianus and R. prolixus have two digestive CABs which are expressed in the same extension as CALs, in the first digestive section of the midgut, but less than in the other midgut sections. Mahanarva fimbriolata does not seem to have digestive CALs and their digestive CABs are mainly expressed in the first digestive section of the midgut and do not diverge much from their lysosomal counterparts. The data suggest that CABs are necessary at the initial stage of digestion in CP-dependent Hemipterans, which action is completed by CALs with low peptide-bond selectivity in Heteroptera species. In M. fimbriolata protein digestion is supposed to be associated with the inactivation of sap noxious proteins, making CAB sufficient as digestive CP. Hemipteran genomes and transcriptome data showed that CALs have been recruited as digestive enzymes only in heteropterans, whereas digestive CABs occur in all hemipterans.
André C Pimentel, Renata O Dias, Thaís D Bifano, Fernando A Genta, Clelia Ferreira, Walter R Terra

1837 related Products with: Cathepsins L and B in Dysdercus peruvianus, Rhodnius prolixus, and Mahanarva fimbriolata. Looking for enzyme adaptations to digestion.

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#33080117   2020/10/20 To Up

Optic, trigeminal, and facial neuropathy related to anti-neurofascin 155 antibody.

To characterize the frequency and patterns of optic, trigeminal, and facial nerve involvement by neuroimaging and electrophysiology in IgG4 anti-neurofascin 155 antibody-positive (NF155 ) chronic inflammatory demyelinating polyneuropathy (CIDP).
Hidenori Ogata, Xu Zhang, Saeko Inamizu, Ken-Ichiro Yamashita, Ryo Yamasaki, Takuya Matsushita, Noriko Isobe, Akio Hiwatashi, Shozo Tobimatsu, Jun-Ichi Kira

2039 related Products with: Optic, trigeminal, and facial neuropathy related to anti-neurofascin 155 antibody.

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#33079306   2020/10/20 To Up

Ribosomal DNA localization on Lathyrus species chromosomes by FISH.

Fluorescence In Situ Hybridization (FISH) played an essential role to locate the ribosomal RNA genes on the chromosomes that offered a new tool to study the chromosome structure and evolution in plant. The 45S and 5S rRNA genes are independent and localized at one or more loci per the chromosome complement, their positions along chromosomes offer useful markers for chromosome discriminations. In the current study FISH has been performed to locate 45S and 5S rRNA genes on the chromosomes of nine Lathyrus species belong to five different sections, all have chromosome number 2n=14, Lathyrus gorgoni Parl, Lathyrus hirsutus L., Lathyrus amphicarpos L., Lathyrus odoratus L., Lathyrus sphaericus Retz, Lathyrus incospicuus L, Lathyrus paranensis Burkart, Lathyrus nissolia L., and Lathyrus articulates L.
Hoda B M Ali, Samira A Osman

1139 related Products with: Ribosomal DNA localization on Lathyrus species chromosomes by FISH.

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#33078992   2020/10/20 To Up

Gender (Im)balance in Citation Practices in Cognitive Neuroscience.

In the field of neuroscience, despite the fact that the proportion of peer-reviewed publications authored by women has increased in recent decades, the proportion of citations of women-led publications has not seen a commensurate increase: In five broad-scope journals, citations of articles first- and/or last-authored by women have been shown to be fewer than would be expected if gender was not a factor in citation decisions [Dworkin, J. D., Linn, K. A., Teich, E. G., Zurn, P., Shinohara, R. T., & Bassett, D. S. The extent and drivers of gender imbalance in neuroscience reference lists. , , 918-926, 2020]. Given the important implications that such underrepresentation may have on the careers of women researchers, it is important to determine whether this same trend is true in subdisciplines of the field, where interventions might be more targeted. Here, we report the results of an extension of the analyses carried out by Dworkin et al. (2020) to citation patterns in the . The results indicate that the underrepresentation of women-led publications in reference sections is also characteristic of articles published in over the past decade. Furthermore, this pattern of citation imbalances is present regardless of author gender, implicating systemic factors. These results contribute to the growing body of evidence that intentional action is needed to address inequities in the way that we carry out and communicate our science.
Jacqueline M Fulvio, Ileri Akinnola, Bradley R Postle

1203 related Products with: Gender (Im)balance in Citation Practices in Cognitive Neuroscience.

100 μg10 100 μg20 100 assays100 ml1 pce96 wells (1 kit)

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#33078719   2020/10/19 To Up

Clinical features of Epstein-Barr virus in the intestinal mucosa and blood of patients with inflammatory bowel disease.

The role of Epstein-Barr virus (EBV) in inflammatory bowel disease (IBD) remains to be elucidated. The aim of this study was to investigate the presence of EBV in the blood and intestinal mucosa of patients with IBD and evaluate the association between EBV positivity and IBD.
Jin-Qiu Zhou, Li Zeng, Qiao Zhang, Xin-Yao Wu, Meng-Lan Zhang, Xing-Tao Jing, Yu-Fang Wang, Hua-Tian Gan

2117 related Products with: Clinical features of Epstein-Barr virus in the intestinal mucosa and blood of patients with inflammatory bowel disease.

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