Only in Titles

Search results for: sensitive

paperclip

#34525464   2021/09/15 To Up

Magneto- and opto-stimuli responsive nanofibers as a controlled drug delivery system.

The drawbacks of conventional drug administration include repeated administration, non-specific biodistribution in the body's systems, the long-term unsustainability of drug molecules, and high global cytotoxicity, posing a challenge for the efficient treatment of chronic diseases that require varying drug dosages over time for optimal therapeutic efficacy. Most controlled release methods encapsulate drug molecules in biodegradable materials that dissolve over time to release the drug, making it difficult to deliver drugs on a schedule. To address these limitations, we developed a magneto-, opto-stimuli responsive drug delivery system based on functionalized electrospun nanofibers loaded with superparamagnetic iron oxide nanoparticles (SPIONs). We exploited the Néel relaxation effect of SPIONs, where heat generated from vibrating SPIONs under exogenously applied magnetic fields or laser illumination induced structural changes of the thermo-sensitive nanofibers that encapsulate the particles. We showed that this structural change of nanofibers is the governing factor in controlling the release of dye molecules, used as a model drug and co-encapsulated within the nanofibers. We also showed that the degree of nanofiber structural change depends on SPION loading and duration of stimulation, demonstrating the tunability of drug release profile. Overall, we demonstrated the potential of SPION-embedded thermoplastic nanofibers as an attractive platform for on-demand drug delivery.
Aihik Banerjee, Tanvi Jariwala, Youn-Kyung Baek, Dung T To, Youyi Tai, Junze Liu, Hyle Park, Nosang V Myung, Jin Nam

2464 related Products with: Magneto- and opto-stimuli responsive nanofibers as a controlled drug delivery system.

100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays100 assays

Related Pathways

paperclip

#34525448   2021/08/30 To Up

Biomechanical assessment of two types and two different locations of subtalar arthroereisis implants for flexible flatfoot: A cadaveric study.

Subtalar arthroereisis refers to the implantation of a sinus tarsi implant for the treatment of flexible flatfoot. The purpose of this study was to compare the ability to correct the flatfoot deformity and contact pressure of the posterior subtalar joint between two types of self-locking wedge implants and between two different positions for the same device in a cadaveric flatfoot model.
Sen Wang, Jian Yu, Maimaitirexiati Helili, Chao Zhang, Jiazhang Huang, Xu Wang, Li Chen, Xin Ma

2012 related Products with: Biomechanical assessment of two types and two different locations of subtalar arthroereisis implants for flexible flatfoot: A cadaveric study.

20 ug100ug Lyophilized25 mg100ug Lyophilized 5 G 1 G 1 kit(s) 100ug

Related Pathways

paperclip

#34525438   2021/09/06 To Up

Artificial light pollution inhibits plant phenology advance induced by climate warming.

Natural photic regime has been drastically altered by the artificial night sky luminance. Despite evidence of sufficient light brightness inducing plant physiology and affecting phenology, generalization regarding effects of light pollution on plant phenology across species and locations is less clear. Meanwhile, the relative contributions and joint effects of artificial light pollution and climate change or other anthropic stressors still remain unknown. To fill this knowledge gap, we utilized in situ plant phenological observations of seven tree species during 1991-2015 in Europe, night-time light dataset and gridded temperature dataset to investigate the impacts of the artificial light pollution on spatial-temporal shifts of plant phenological phases under climatic warming. We found 70% of the observation sites were exposed to increased light pollution during 1992-2015. Among them, plant phenological phases substantially delayed at 12-39% observation sites of leaf-out, and 6-53% of flowering. We also found plant species appeared to be more sensitive to artificial light pollution, and phenology advancement was hindered more prominently and even delay phenomenon exhibited when the color level showed stronger sky brightness. Linear mixed models indicate that although temperature plays a dominant role in shifts of plant phenological phases at the spatial scale, the inhibitory effect of artificial light pollution is evident considering the interactions. To our knowledge, this study is the first to quantitatively establish the relationship between artificial light pollution and plant phenology across species and locations. Meanwhile, these findings provide a new insight into the ecological responses of plant phenology to the potential but poorly understood environmental stressors under this warmer world and call for light pollution to be accorded the equal status as other global change phenomena.
Xihong Lian, Limin Jiao, Jing Zhong, Qiqi Jia, Jiafeng Liu, Zejin Liu

1787 related Products with: Artificial light pollution inhibits plant phenology advance induced by climate warming.

50 100 mg1 ml10 mg 6 ml Ready-to-use 1 mg100ug100 ul200ul

Related Pathways

paperclip

#34525387   2021/09/12 To Up

In situ label-free and sensitive detection assay for cell apoptosis via polyadenosine-coralyne fluorescence enhancement strategy.

Cell apoptosis detection is vital for biological analysis and clinical application; some detection assays are already commercially available. However, it is still far from perfect and needs further improvement for less cost, time-consuming and operation demanding. TUNEL, a high market share cell apoptosis assay, depends on adulteration fluorescent labelling dUTP by terminal deoxynucleotidyl transferase(TdT) which randomly adds deoxyribonucleoside triphosphates (dNTPs) at the 3'-OH terminal of ssDNA with a template-free manner. Based on our previous work, we adopted a label-free strategy to reduce the cost and operation maintenance of TUNEL and developed a facile, rapid, convenient and in-situ assay for cell apoptosis.
Chun Wu, Jingru Wang, Yihang Chen, Xiwen Xing

1224 related Products with: In situ label-free and sensitive detection assay for cell apoptosis via polyadenosine-coralyne fluorescence enhancement strategy.

1 kit1 kit1 kit100 Tests1 kit1 kit100 Tests1 kit1 kit1 kit1 kit1 kit

Related Pathways

paperclip

#34525346   2021/09/14 To Up

RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy.

Doxorubicin is an anthracycline chemotherapy agent effective in treating a wide range of malignancies, but its use is limited by dose-dependent cardiotoxicity. A recent genome-wide association study identified a SNP (rs2229774) in retinoic acid receptor-γ (RARG) as statistically associated with increased risk of anthracycline-induced cardiotoxicity. Here, we show that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from patients with rs2229774 and who suffered doxorubicin-induced cardiotoxicity (DIC) are more sensitive to doxorubicin. We determine that the mechanism of this RARG variant effect is mediated via suppression of topoisomerase 2β (TOP2B) expression and activation of the cardioprotective extracellular regulated kinase (ERK) pathway. We use patient-specific hiPSC-CMs as a drug discovery platform, determining that the RARG agonist CD1530 attenuates DIC, and we confirm this cardioprotective effect in an established in vivo mouse model of DIC. This study provides a rationale for clinical prechemotherapy genetic screening for rs2229774 and a foundation for the clinical use of RARG agonist treatment to protect cancer patients from DIC.
Tarek Magdy, Zhengxin Jiang, Mariam Jouni, Hananeh Fonoudi, Davi Lyra-Leite, Gwanghyun Jung, Marisol Romero-Tejeda, Hui-Hsuan Kuo, K Ashley Fetterman, Mennat Gharib, Brian T Burmeister, Mingming Zhao, Yadav Sapkota, Colin J Ross, Bruce C Carleton, Daniel Bernstein, Paul W Burridge

1987 related Products with: RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy.

100ug50 ul 100ul0.1 mg96 assays10 mg100 ul100ug100 ul100ug100ug50 ul

Related Pathways

paperclip

Error loading info... Pleas try again later.
paperclip

#34525229   2021/09/15 To Up

Altered albedo dominates the radiative forcing changes in a subtropical forest following an extreme snow event.

Subtropical forests are important ecosystems globally due to their extensive role in carbon sequestration. Extreme climate events are known to introduce disturbances in the ecosystem that cause long-term changes in carbon balance and radiation reflectance. However, how these ecosystem function changes contribute to global warming in terms of radiative forcing (RF), especially in the years following a disturbance, still needs to be investigated. We studied an extreme snow event that occurred in a subtropical evergreen broad-leaved forest in southwestern China in 2015 and used 9 years (2011-2019) of net ecosystem CO exchange (NEE) and surface albedo (α) data to investigate the effect of the event on the ecosystem RF changes. In the year of the disturbance, leaf area index (LAI) declined by 40% and α by 32%. The annual NEE was -718 ± 128 gC m as a sink in the pre-disturbance years (2011-2014), but after the event, the sink strength dropped significantly by 76% (2015). Both the vegetation, indicated by LAI, and α recovered to pre-disturbance levels in the fourth post-disturbance year (2018). However, the NEE recovery lagged and occurred a year later in 2019, suggesting a more severe and lasting impact on the ecosystem carbon balance. Overall, the extreme event caused a positive (warming effect) net RF which was predominantly caused by changes in α (90-93%) rather than those in NEE. This result suggests that, compared to the climate effect caused by forest carbon sequestration changes, the climate effect of α alterations can be more sensitive to vegetation damage induced by natural disturbances. Moreover, this study demonstrates the important role of vegetation recovery in driving canopy reflectance and ecosystem carbon balance during the post-disturbance period, which determines the ecosystem feedbacks to the climate change.
Gnanamoorthy Palingamoorthy, Qing-Hai Song, Zhao Junbin, Yiping Zhang, Yuntong Liu, Wenjun Zhou, Liqing Sha, Zexin Fan, Pramit Kumar Deb Burman

1601 related Products with: Altered albedo dominates the radiative forcing changes in a subtropical forest following an extreme snow event.

100 μg4 Arrays/Slide1.00 mg1 Set1 Set

Related Pathways

paperclip

Error loading info... Pleas try again later.
paperclip

#34525112   2021/09/15 To Up

Effect of high and low risk susceptibles in the transmission dynamics of COVID-19 and control strategies.

In this study, we formulate and analyze a deterministic model for the transmission of COVID-19 and evaluate control strategies for the epidemic. It has been well documented that the severity of the disease and disease related mortality is strongly correlated with age and the presence of co-morbidities. We incorporate this in our model by considering two susceptible classes, a high risk, and a low risk group. Disease transmission within each group is modelled by an extension of the SEIR model, considering additional compartments for quarantined and treated population groups first and vaccinated and treated population groups next. Cross Infection across the high and low risk groups is also incorporated in the model. We calculate the basic reproduction number [Formula: see text] and show that for [Formula: see text] the disease dies out, and for [Formula: see text] the disease is endemic. We note that varying the relative proportion of high and low risk susceptibles has a strong effect on the disease burden and mortality. We devise optimal medication and vaccination strategies for effective control of the disease. Our analysis shows that vaccinating and medicating both groups is needed for effective disease control and the controls are not very sensitive to the proportion of the high and low risk populations.
Adnan Khan, Mohsin Ali, Wizda Iqbal, Mudassar Imran

1269 related Products with: Effect of high and low risk susceptibles in the transmission dynamics of COVID-19 and control strategies.

2 Pieces/Box4 Arrays/Slide2 Pieces/Box10 mg4 Membranes/Box2 Pieces/Box100ug

Related Pathways

paperclip

#34525109   2021/09/15 To Up

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.

There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not have the capacity to develop these and/or perform manual processing. We provide multiple workflows for SARS-CoV-2 nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral RNA Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind Viral DNA/RNA 96 Kit (Omega Bio-tek). We also compared One-step RT-qPCR reagents: TaqMan Fast Virus 1-Step Master Mix (FastVirus, ThermoFisher Scientific), qPCRBIO Probe 1-Step Go Lo-ROX (PCR Biosystems) and Luna® Universal Probe One-Step RT-qPCR Kit (Luna, NEB). We used primer-probes that detect viral N (EUA CDC) and RdRP. RNA extraction methods provided similar results, with Beckman performing better with our primer-probe combinations. Luna proved most sensitive although overall the three reagents did not show significant differences. N detection was more reliable than that of RdRP, particularly in samples with low viral titres. Importantly, we demonstrated that heat treatment of nasopharyngeal swabs at 70°C for 10 or 30 min, or 90°C for 10 or 30 min (both original variant and B 1.1.7) inactivated SARS-CoV-2 employing plaque assays, and had minimal impact on the sensitivity of the qPCR in clinical samples. These findings make SARS-CoV-2 testing portable in settings that do not have CL-3 facilities. In summary, we provide several testing pipelines that can be easily implemented in other laboratories and have made all our protocols and SOPs freely available at https://osf.io/uebvj/.
Maria Jose Lista, Pedro M Matos, Thomas J A Maguire, Kate Poulton, Elena Ortiz-Zapater, Robert Page, Helin Sertkaya, Ana M Ortega-Prieto, Edward Scourfield, Aoife M O'Byrne, Clement Bouton, Ruth E Dickenson, Mattia Ficarelli, Jose M Jimenez-Guardeño, Mark Howard, Gilberto Betancor, Rui Pedro Galao, Suzanne Pickering, Adrian W Signell, Harry Wilson, Penelope Cliff, Mark Tan Kia Ik, Amita Patel, Eithne MacMahon, Emma Cunningham, Katie Doores, Monica Agromayor, Juan Martin-Serrano, Esperanza Perucha, Hannah E Mischo, Manu Shankar-Hari, Rahul Batra, Jonathan Edgeworth, Mark Zuckerman, Michael H Malim, Stuart Neil, Rocio Teresa Martinez-Nunez

1952 related Products with: Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.

1mg1mg100 ug/vial200ul1mg1mg100 ug/vial1mg 5 G100ug Lyophilized

Related Pathways