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#39494294   2024/10/29 To Up

Forkhead box D subfamily genes in colorectal cancer: potential biomarkers and therapeutic targets.

The forkhead box (FOX) family members regulate gene transcription and expression. FOX family members regulate various biological processes, such as cell proliferation and tumorigenesis. FOXD, a FOX protein subfamily, is associated with poor prognosis for various cancers. However, the potential clinical value of FOXD subfamily members in colorectal cancer (CRC) has not yet been elucidated. Therefore, in this study, we aimed to determine the role of the FOXD subfamily members in CRC development.
Ying Chen, Haiyan Qiao, Ruiqi Zhong, Lei Sun, Bingbing Shang

2161 related Products with: Forkhead box D subfamily genes in colorectal cancer: potential biomarkers and therapeutic targets.



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#39492445   2023/10/28 To Up

Effect of the HCT Gene on Lignin Synthesis and Fiber Development in Gossypium barbadense.

As one of the key enzymes in the metabolic pathway of phenylpropane, shikimate hydroxycinnamoyl transferase (HCT) is mainly involved in the biosynthesis of the plant secondary cell wall, which is closely related to cotton fiber quality. In this study, whole-genome identification and bioinformatics analysis of the HCT gene family were performed in G. barbadense. In the whole genome, we identified 136 GbHCT genes encoding 309-504 amino acids. Phylogenetic analysis divided the genome into 5 subfamilies, which were located on 25 chromosomes. Collinear analysis of polyploidization and tandem duplication events were the main driving forces for the rapid expansion and evolution of this family, and the genes underwent loose purifying selection constraints after duplication. Gene promoters identified a variety of cis-acting elements related to plant hormones and the stress response. Several members of the GbHCT family were highly expressed during the development of cotton fiber, and different members had different expression patterns in cotton fiber. After GbHCT114 gene silencing in cotton, the amount of stem surface trichomes and lignin content decreased, and the cell morphology and arrangement changed. After the GbHCT114 gene was overexpressed in Arabidopsis thaliana (L.) Heynh., the number of stem and leaf surface trichomes and the cross-sectional area of the secondary xylem duct cell wall increased. In addition, utilizing transcriptomic analysis, differentially expressed genes associated with lignin synthesis and fiber development were identified. Taken together, the results obtained in this study confirm that the GbHCT114 gene regulates plant trichome development, which lays a theoretical foundation for future studies on the function of GbHCT114 in cotton.
Kai Zheng, Yongsheng Cai, Yanying Qu, Lu Teng, Chaoyue Wang, Jie Gao, Quanjia Chen

2337 related Products with: Effect of the HCT Gene on Lignin Synthesis and Fiber Development in Gossypium barbadense.

300 units100ug96T96T5ug10.1ml (1mg/ml)

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#39491259   2023/10/28 To Up

The Armillaria response to Gastrodia elata is partially mediated by strigolactone-induced changes in reactive oxygen species.

Armillaria root diseases, caused by Armillaria spp., pose a significant threat to woody plants worldwide and result in substantial economic losses. However, certain species in the genus Armillaria can establish a unique symbiotic relationship with Gastrodia elata, which is the only known example of a plant benefiting from Armillaria. Although various plant signals that play a role in this interaction have been identified, the mechanism remains largely unknown from the Armillaria's perspective. In this study, we performed whole-genome sequencing of an Armillaria gallica strain named NRC001 isolated from G. elata. Comparative genomic analysis showed it is low-pathogenic Armillaria spp., which possesses 169 expanded gene families compared to high-pathogenic Armillaria spp. Among these expanded families, transcriptomic analysis revealed a significant increase in expression levels of four reactive oxygen species (ROS)-related gene families in A. gallica on G. elata compared to A. gallica on wood. Thus, a systematic survey of ROS-related gene families was carried out, and a total of 218 genes belonging to 44 ROS-related gene families in A. gallica were identified. Physiological experiments and transcriptome analysis showed that strigolactones (SLs) released by G. elata have a mediation impact on ROS, particularly enhancing the ROS scavenging activities by increasing the expression level and activity of several enzymes, such as catalase and glutathione reductase. Among the ROS-related genes, the aquaporin (AQP) is crucial as it is responsible for transporting hydrogen peroxide (HO) across the cell membrane. Five orthologs of AQP genes in A. gallica were identified and overexpressed in yeast. Only AgAQPA from the so-called 'other aquaglyceroporin' subfamily was demonstrated to be capable of mediating HO transport in A. gallica. To our best knowledge, this is the first 'other aquaglyceroporins' gene in fungi to be identified as having transporter capacity. This study not only provides new insights into the mechanisms by which SL signaling regulates interactions between Armillaria and G. elata, but also sheds light on the function of fungal AQPs.
Zhongyi Hua, Xiying Teng, Jingwen Huang, Junhui Zhou, Yuyang Zhao, Luqi Huang, Yuan Yuan

1262 related Products with: The Armillaria response to Gastrodia elata is partially mediated by strigolactone-induced changes in reactive oxygen species.

50 ul50 ul 100ul100 ul96T 100ul100 ul

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#39491176   2023/10/27 To Up

Chromosome-level genome assembly of Bactrocera correcta provides insights into its adaptation and invasion mechanisms.

Bactrocera correcta is an invasive polyphagous pest with significant ecological and economic implications. Understanding its genetic characteristics and the molecular mechanisms that drive its rapid adaptation to new environments requires genomic information. In this study, we successfully assembled the chromosome-level genome of B. correcta using PacBio long-read sequencing, Illumina sequencing, and chromatin conformation capture (Hi-C) methods. The final genome assembly spans a total length of 702.65 Mb. We managed to anchor approximately 86.88% of the assembled contigs into 6 linkage groups, ranging from 17.97 Mb to 166.49 Mb. Additionally, our analysis predicted a total of 21,015 genes, with repetitive sequences accounting for 58.22% of the genome. We further identified retroelements and DNA transposons as the major contributors to the larger size of the B. correcta genome, constituting 36.06% and 30.92% of the repetitive sequences, respectively. Our divergence time estimation placed B. correcta's split from other Bactrocera species at around 5.99-16.71 million years ago. Through gene family analyses, we discovered significant expansions in sensing-related gene families (IR, GR), heat shock proteins (HSP60), and resistance-related gene families (ABC) in B. correcta compared to its closest relatives. Transcriptomic analysis revealed substantial upregulation of HSP genes, especially those from the HSP20 subfamily, in response to high temperatures. The availability of this reference genome serves as a foundation for the identification of precise target genes in B. correcta, facilitating molecular prevention and control strategies.
Tengda Guo, Shiqian Feng, Yue Zhang, Weisong Li, Yujia Qin, Zhihong Li

2650 related Products with: Chromosome-level genome assembly of Bactrocera correcta provides insights into its adaptation and invasion mechanisms.

5mg50mg10mg100ug5mg5mg300 units1 g50 ug 10mg5mg96 wells (1 kit)

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#39490648   2024/10/26 To Up

Genome-wide analysis of the MADS-box gene family in mango and ectopic expression of MiMADS77 in Arabidopsis results in early flowering.

Mango (Mangifera indica L.) is an important tropical fruit, and timely flowering and fruit setting are very important for mango production. The MADS-box gene family is involved in the regulation of flower induction, floral organ specification, and fruit development in plants. The identification and analysis of the MADS-box gene family can lay a foundation for the study of the molecular mechanism of flowering and fruit development in mango. In this study, 119 MiMADS-box genes were identified on the basis of genome and transcriptome data. Phylogenetic analysis revealed that these genes can be divided into two classes. Forty-one type I proteins were further divided into three subfamilies, and seventy-eight type II proteins were further classified into eleven subfamilies. Several pairs of alternative splicing genes were found, especially in the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) subfamily. The MiMADS-box genes were distributed on 18 out of the 20 mango chromosomes. Cis-element analysis revealed many light-, stress-, and hormone-responsive elements in the promoter regions of the mango MiMADS-box genes. Expression pattern analysis revealed that these genes were differentially expressed in multiple tissues in mango. The highly expressed MiMADS77 was subsequently transformed into Arabidopsis, resulting in significant early flowering and abnormal floral organs. Yeast two-hybrid (Y2H) assays revealed that MiMADS77 interacts with several MiMADS-box proteins. In addition, we constructed a preliminary flowering regulatory network of MADS-box genes in mango on the basis of related studies. These results suggest that MiMADS77 genes may be involved in flowering regulation of mango.
Haixia Yu, Liming Xia, Jiawei Zhu, Xiaojie Xie, Ying Wei, Xi Li, Xinhua He, Cong Luo

2611 related Products with: Genome-wide analysis of the MADS-box gene family in mango and ectopic expression of MiMADS77 in Arabidopsis results in early flowering.

300 units96T96T7 x 25 ul1roll (50 m)5mg100 ul

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#39489865   2024/11/03 To Up

Oral N-acetylcysteine ameliorates liver fibrosis and enhances regenerative responses in Mdr2 knockout mice.

Cholangiopathies are poorly understood disorders with no effective therapy. The extrahepatic biliary tree phenotype is less studied compared to the intrahepatic biliary injury in both human disease and Mdr2 mice, the established cholestatic mouse model. This study aimed to characterize the extra hepatic biliary tree of Mdr2 mice at various ages and to determine if injury can be repaired with the antioxidant and glutathione precursor N-acetyl-L-Cysteine treatment (NAC). We characterized extra hepatic bile ducts (EHBD)s at various ages from 2 to 40 weeks old FVB/N and Mdr2 mice. We examined the therapeutic potential of local NAC ex vivo using EHBD explants at early and late stages of injury; and systematic therapy by in vivo oral administration for 3 weeks. EHBD and liver sections were assessed by histology and immunofluorescent stains. Serum liver enzyme activities were analyzed, and liver spatial protein expression analysis was performed. Mdr2 mice developed progressive EHBD injury, similar to extrahepatic PSC. NAC treatment of ex vivo EHBD explants led to improved duct morphology. In vivo, oral administration of NAC improved liver fibrosis, and decreased liver enzyme activities. Spatial protein analysis revealed cell-type specific differential response to NAC, collectively indicating a transition from pro-apoptotic into proliferative state. NAC treatment should be further investigated as a potential therapeutic option for human cholangiopathies.
Adi Har-Zahav, Ana Tobar, Sophia Fried, Rachel Sivan, Benjamin J Wilkins, Pierre Russo, Raanan Shamir, Rebecca G Wells, Michael Gurevich, Orith Waisbourd-Zinman

2918 related Products with: Oral N-acetylcysteine ameliorates liver fibrosis and enhances regenerative responses in Mdr2 knockout mice.



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#39489264   2024/11/01 To Up

ChBBX6 and ChBBX18 are positive regulators of anthocyanins biosynthesis and carotenoids degradation in Cerasus humilis.

B-box zinc-finger transcription factor (BBX) plays important regulatory roles in plant secondary metabolism. Here, we identified 21 BBXs that could be further categorized into five subfamilies from Cerasus humilis. Two segmentally duplicated Subfamily IV members, ChBBX6 and ChBBX18, were found to share high homology with reported anthocyanin-related BBXs and express highly in fruits with high anthocyanins but low carotenoids contents. Their transient overexpression in apple and C. humilis fruits both led to significantly increased anthocyanins accumulation and significantly upregulated expression of anthocyanins-related genes. However, their overexpression resulted in decreased carotenoids accumulation and greatly upregulated the expression of carotenoids-related genes especially degradation-related genes. Additionally, their overexpression both greatly improved the ABA content in C. humilis fruits. Through yeast one-hybrid and dual-luciferase reporter assays, we found that both ChBBX6 and ChBBX18 could bind to and activate the promoters of chalcone synthase (ChCHS), flavanone 3-hydroxylase (ChF3H), and 9-cis-epoxycarotenoid dioxygenase 5 (ChNCED5). Our study demonstrates that ChBBX6 and ChBBX18 are positive regulators of anthocyanins biosynthesis and carotenoids degradation and can provide basis for understanding the roles of BBX genes in C. humilis.
Yang Hu, Zhiqian Gong, Yiming Yan, Jiating Zhang, Anping Shao, Hao Li, Pengfei Wang, Shuai Zhang, Chunzhen Cheng, Jiancheng Zhang

2802 related Products with: ChBBX6 and ChBBX18 are positive regulators of anthocyanins biosynthesis and carotenoids degradation in Cerasus humilis.

100 mg100ul1 ml200ul10 mg2.5 mgcase 25 MG100ul25 mg100 mg

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#39488803   2024/11/03 To Up

Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2024).

This article reports changes to virus taxonomy and taxon nomenclature that were approved and ratified by the International Committee on Taxonomy of Viruses (ICTV) in April 2024. The entire ICTV membership was invited to vote on 203 taxonomic proposals that had been approved by the ICTV Executive Committee (EC) in July 2023 at the 55th EC meeting in Jena, Germany, or in the second EC vote in November 2023. All proposals were ratified by online vote. Taxonomic additions include one new phylum (Ambiviricota), one new class, nine new orders, three new suborders, 51 new families, 18 new subfamilies, 820 new genera, and 3547 new species (excluding taxa that have been abolished). Proposals to complete the process of species name replacement to the binomial (genus + species epithet) format were ratified. Currently, a total of 14,690 virus species have been established.
Peter Simmonds, Evelien M Adriaenssens, Elliot J Lefkowitz, Hanna M Oksanen, Stuart G Siddell, Francisco Murilo Zerbini, Poliane Alfenas-Zerbini, Frank O Aylward, Donald M Dempsey, Bas E Dutilh, Juliana Freitas-Astúa, María Laura García, R Curtis Hendrickson, Holly R Hughes, Sandra Junglen, Mart Krupovic, Jens H Kuhn, Amy J Lambert, Małgorzata Łobocka, Arcady R Mushegian, Judit Penzes, Alejandro Reyes Muñoz, David L Robertson, Simon Roux, Luisa Rubino, Sead Sabanadzovic, Donald B Smith, Nobuhiro Suzuki, Dann Turner, Koenraad Van Doorslaer, Anne-Mieke Vandamme, Arvind Varsani

1019 related Products with: Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2024).

100.00 ul1,000 IU1 ml50 IU11500 Units

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#39488543   2024/11/02 To Up

Genome-wide characterization of PEBP genes in Mung bean (Vigna radiata L.) with functional analysis of VrFT1 in relation to photoperiod.

Mung bean (Vigna radiata L.), a widely cultivated legume, belongs to the Fabaceae family's Papilionoideae subfamily. Although Phosphatidylethanolamine-binding protein (PEBP) genes have been identified in several plant species, their presence and function in mung bean remain largely unexplored. In this study, we identified seven VrPEBP genes from mung bean and classified them into four clades: FT, MFT, TFL and FT-like. Cis-regulatory element analysis revealed that VrPEBP genes may play a role in light, hormone, and stress responses. Quantitative real-time PCR (qRT-PCR) analysis indicated that VrPEBPs were constitutively expressed in various tissues. However, tissue-specific expression patterns were observed among VrPEBP genes. Under short-day (SD) conditions, VrFT1 and VrMFT1 exhibited significantly higher expression levels than under long-day (LD) conditions at 8 and 4 h, respectively. Conversely, VrTFL2 and VrTFL3 showed significantly higher expression levels under LD conditions compared to SD conditions at 8 and 12 h, respectively. The varied expression patterns of these genes under different photoperiod suggest their potential involvement in the photoperiodic regulation of flowering in mung bean. Additionally, phenotypic analysis of transgenic Arabidopsis plants overexpressing VrFT1 revealed higher expression levels under SD conditions and predicted its role in promoting flowering. These results provide valuable insights into the evolution and function of PEBP genes in mung bean and lay the foundation for further research on their regulatory mechanisms and potential applications in mung bean improvement.
Renfeng Xue, Yu Liu, Ming Feng, Yuning Huang, Yang Zhao, Jian Chen, Tao Li, Chao Zhong, Weide Ge

1670 related Products with: Genome-wide characterization of PEBP genes in Mung bean (Vigna radiata L.) with functional analysis of VrFT1 in relation to photoperiod.

100 μg100 mg5096 wells (1 kit)500 gm.101 kit100

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#39480815   2024/10/31 To Up

Mutational analysis of Phanerochaete chrysosporium´s purine transporter.

We present here a mutational analysis of the purine transporter from Phanerochaete chrysosporium (PhZ), a member of the AzgA-like subfamily within the Nucleobase Ascorbate Transporters family. We identified key residues that determine its substrate specificity and transport efficiency. Thirteen PhZ mutants were generated and heterologously expressed in Aspergillus nidulans. The growth of mutant strains in the presence of purines and toxic analogues and the uptake rate of radiolabelled hypoxanthine were evaluated. Results revealed that ten mutants showed differences in transport compared to the wild-type PhZ: six mutants completely lost function, two exhibited decreased transport activity, and two showed increased hypoxanthine uptake. Subcellular localization and expression level analyses indicated that the differences in transport activity were not due to trafficking issues to the plasma membrane or protein stability. A three-dimensional model of PhZ, constructed with the artificial intelligence-based AlphaFold2 program, suggested that critical residues for transport are located in transmembrane segments and an internal helix. In the latter, the A418 residue was identified as playing a pivotal role in transport efficiency despite being far from the putative substrate binding site, as mutant A418V showed an increased initial uptake efficiency for the transporter´s physiological substrates. We also report that residue L124, which lies in the putative substrate binding site, plays a critical role in substrate transport, emerging as an additional determinant in the transport mechanism of this family of transporters. These findings underscore the importance of specific residues in AzgA-like transporters and enhance our understanding of the intricate mechanisms governing substrate specificity and transport efficiency within this family.
Mariana Barraco-Vega, Manuel Sanguinetti, Gabriela da Rosa, Gianna Cecchetto

2427 related Products with: Mutational analysis of Phanerochaete chrysosporium´s purine transporter.

1 kit(s) 100ug50 100ug50 ug 100ulBox Of 125 Strips/Unit25 mg

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