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#36473054   2022/12/06 To Up

Chromosome-scale Amaranthus tricolor genome provides insights into the evolution of the genus Amaranthus and the mechanism of betalain biosynthesis.

Amaranthus tricolor, is a vegetable and ornamental amaranth, with high lysine, dietary fiber and squalene content. The red cultivar of A. tricolor possesses a high concentration of betalains, which has been used as natural food colorants. Here, we constructed the genome of A. tricolor, the first reference genome for the subgenus Albersia, combining PacBio HiFi, Nanopore ultra-long and Hi-C data. The contig N50 size was 906 Kb, and 99.58% of contig sequence was anchored to the 17 chromosomes, totaling 520 Mb. We annotated 27,813 protein-coding genes with an average 1.3 Kb coding sequence and 5.3 exons. We inferred that A. tricolor underwent a whole-genome duplication (WGD) and that the WGD shared by amaranths occurred in the last common ancestor of subfamily Amaranthoideae. Moreover, we comprehensively identified candidate genes in betalain biosynthesis pathway. Among them, DODAα1 and CYP76ADα1, located in one topologically associated domain (TAD) of an active (A) compartment on chromosome 16, were more highly expressed in red leaves than in green leaves, and DODAα1 might be the rate-limiting enzyme gene in betalains biosynthesis. This study presents new genome resources and enriches our understanding of amaranth evolution, betalains production, facilitating molecular breeding improvements and the understanding of C4 plants evolution.
Hengchao Wang, Dong Xu, Sen Wang, Anqi Wang, Lihong Lei, Fan Jiang, Boyuan Yang, Lihua Yuan, Rong Chen, Yan Zhang, Wei Fan

2461 related Products with: Chromosome-scale Amaranthus tricolor genome provides insights into the evolution of the genus Amaranthus and the mechanism of betalain biosynthesis.

1500 Units1250 IU100.00 ul1500 Units

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#36472002   // To Up

[Cloning of StHD1 and StHD8 from Schizonepeta tenuifolia and function of regulating glandular trichome development].

Hd-Zip, a unique transcription factor in plant kingdom, influences the growth, development, and secondary metabolism of plants. Hd-zip Ⅳ is thought to play an important role in trichome development of Schizonepeta tenuifolia. This study aims to explore the functions of StHD1 and StHD8 in Hd-zip Ⅳ subfamily in peltate glandular trichome development. To be specific, the expression patterns of the two genes and interaction between the proteins encoded by them were analyzed based on transcriptome sequencing and two-hybrid screening. The subcellular localization was performed and functions of the genes were verified in tobacco and S. tenuifolia. The results showed that StHD1 and StHD8 had high similarity to HD-Zip Ⅳ proteins of other plants and they all had the characteristic conserved domains of HD-Zip Ⅳ subfamily. They were located in the nucleus. The two genes mainly expressed in young tissues and spikes, and StHD1 and StHD8 proteins interacted with each other. The density and length of glandular trichomes increased significantly in tobacco plants with the overexpression of StHD1 and StHD8. Inhibiting the expression of StHD1 and StHD8 by VIGS(virus-induced gene silencing) in S. tenuifolia resulted in the reduction in the density of peltate glandular trichomes, the expression of key genes related to mono-terpene synthesis, and the relative content of limonene and pulegone, the main components of monoterpene. These results suggested that StHD1 and StHD8 of S. tenuifolia formed a complex to regulate glandular trichomes and affect the biosynthesis of monoterpenes.
Pei-Na Zhou, Jing-Jie Dang, Yong-Fang Shao, Zun-Rui Shi, Lin Zhang, Chan-Chan Liu, Qi-Nan Wu

2505 related Products with: [Cloning of StHD1 and StHD8 from Schizonepeta tenuifolia and function of regulating glandular trichome development].

1 g96 wells (1 kit)100ug10 mg100 mg100ug0.1 mg200ul25 mg100 mg1000 25 MG

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#36471968   // To Up

[Identification of Carthamus tinctorius NAC gene family and analysis of drought stress response].

The NAC(NAM/ATAF/CUC) transcription factors are members of the largest transcriptional gene family in plants and play an essential role in the response of plants to drought stress. To identify the number and function of the NAC gene family in Carthamus tinctorius, the present study adopted bioinformatics methods to identify NAC gene family members based on the whole genome data of C. tinctorius, and analyzed their physicochemical properties, chromosomal location, phylogenetic relationship, gene structure, conserved domain, and conserved motif. Meanwhile, the real-time fluorescence-based quantitative RT-PCR(qRT-PCR) was used to analyze the transcription level of four NAC genes under drought stress in different time. The results showed that C. tinctorius contained 87 NAC genes unevenly distributed on 11 chromosomes, while no NAC gene was found on chromosome 12. The encoded proteins were 103-974 amino acids and the number of CDS ranged from 3 to 9. According to the phylogenetic relationships, 87 NAC genes were clustered into17 subfamilies. The analysis of conserved domains and motifs revealed that most of the genes contained five conserved subdomains, A-E and motif2 was the most conserved among NAC genes. The expression pattern analysis showed that the transcription levels of four NAC genes related to drought resistance were all up-regulated after drought stress treatment for different time, suggesting that these four NAC genes may be related to drought resistance of C. tinctorius. This study is expected to provide a theoretical basis for further functional analysis of NAC transcription factors in C. tinctorius and references for the cultivation of drought-tolerant C. tinctorius varieties.
Peng Zhan, Zu-Chang Zhong, Ni-Yan Xiang, Rui Qin, Xiong-Bo Jiang, Hong Liu

2481 related Products with: [Identification of Carthamus tinctorius NAC gene family and analysis of drought stress response].

100ug 5 G96T 100 UG96T100ug25 mg50 ug100ug50 ug

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#36471960   // To Up

[Main spicy components, mechanism and masking technology for spicy flavor of Chinese medicine: a review].

Many Chinese medicinal materials, vegetable oils and extracts, and even Chinese patent medicines are spicy, which influences the medication compliance of patients, especially children. Different from the sour, sweet, bitter, salty, and umami tastes, it is a painful sensation formed when the spicy substances stimulate the nerve endings. At the moment, there are a few studies on the spicy components and mechanism and masking technology for the spicy flavor of Chinese medicine in the pharmaceutical industry, and the findings in food science are usually taken as a reference, which fail to guide the masking of the spicy flavor in Chinese medicine preparations. According to literature research, the exterior-releasing medicine, dampness-resolving medicine, and interior-warming medicine are spicy, especially some vegetable oils and extracts. Taking Zingiberis Rhizoma and prescriptions containing this medicinal as an example, the spicy components in Chinese medicine and the structure-activity characteristics were analyzed to reveal the mechanism for the spicy flavor: spicy components activate the transient receptor potential vanilloid subfamily member 1(TRPV1). The advantages and disadvantages of separation, neutralization with sugar, and inclusion for the masking of the spicy flavor were summarized and the applicability in Chinese medicine was analyzed. Moreover, the future development direction was put forward. This study is expected to promote the development of spicy masking technology for Chinese medicine prescriptions for children.
Tai Lu, Zheng-Jiang Xie, Lin Liu, Min Qiu, Ya-Nan He, Li Han, Jun-Zhi Lin, Ding-Kun Zhang

2050 related Products with: [Main spicy components, mechanism and masking technology for spicy flavor of Chinese medicine: a review].

50 ul5mg100 ul100 ul50 ul100ug96 Tests500 mg250 mg1 mg25 mg

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#36471241   2022/12/05 To Up

Plasma membrane aquaporins of the PIP1 and PIP2 subfamilies facilitate hydrogen peroxide diffusion into plant roots.

The permeability of plasma membrane aquaporins (PIPs) to small solutes other than water greatly diversifies their potential functions in plant development and metabolic processes. One such process is stress signalling in which hydrogen peroxide (HO) plays a major role. Based on transport assays carried out in yeast, there are differences in the degree to which PIPs of Arabidopsis thaliana, are permeable to HO and thus they may differentially facilitate transmembrane diffusion. Here, we test whether specific PIPs aid in the transmembrane diffusion of HO to such an extent that knocking-out PIPs affects plant phenotype. We examined changes in growth and morphology, including biomass accumulation, root system architecture and relative water content, as well as gas exchange, across two HO treatments in knockout mutants of A. thaliana.
David Israel, Seong Hee Lee, Thomas Matthew Robson, Janusz Jerzy Zwiazek

2733 related Products with: Plasma membrane aquaporins of the PIP1 and PIP2 subfamilies facilitate hydrogen peroxide diffusion into plant roots.

96T1 kit96T 72 tests200 assays96T1 kit1 lt1 mg200 assays1 kit200 ug

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#36468470   2022/07/25 To Up

Sequential anti-inflammatory and osteogenic effects of a dual drug delivery scaffold loaded with parthenolide and naringin in periodontitis.

Our pilot study showed that a 3-dimensional dual drug delivery scaffold (DDDS) loaded with Chinese herbs significantly increased the regenerated bone volume fraction. This study aimed to confirm the synergistic anti-inflammatory and osteogenic preclinical effects of this system.
Rui Chen, Mengting Wang, Qiaoling Qi, Yanli Tang, Zhenzhao Guo, Shuai Wu, Qiyan Li

2447 related Products with: Sequential anti-inflammatory and osteogenic effects of a dual drug delivery scaffold loaded with parthenolide and naringin in periodontitis.

0.1 mg1000 1 ml100ul100 μg100ul1000 TESTS/0.65ml100.00 ul200 100 μg100ug100 μg

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#36467694   2022/11/16 To Up

Mechano-growth factor E-domain modulates cardiac contractile function through 14-3-3 protein interactomes.

In the heart, alternative splicing of the gene produces two isoforms: IGF-IEa and IGF-IEc, (Mechano-growth factor, MGF). The sequence divergence between their E-domain regions suggests differential isoform function. To define the biological actions of MGF's E-domain, we performed analysis of the unique C-terminal sequence and identified a phosphorylation consensus site residing within a putative 14-3-3 binding motif. To test the functional significance of Ser 18 phosphorylation, phospho-mimetic (S/E) and phospho-null (S/A) peptides were delivered to mice at different doses for 2 weeks. Cardiovascular function was measured using echocardiography and a pressure-volume catheter. At the lowest (2.25 mg/kg/day) and highest (9 mg/kg/day) doses, the peptides produced a depression in systolic and diastolic parameters. However, at 4.5 mg/kg/day the peptides produced opposing effects on cardiac function. Fractional shortening analysis also showed a similar trend, but with no significant change in cardiac geometry. Microarray analysis discovered 21 genes (FDR < 0.01), that were expressed accordant with the opposing effects on contractile function at 4.5 mg/kg/day, with the nuclear receptor subfamily 4 group A member 2 () identified as a potential target of peptide regulation. Testing the regulation of the Nr4a family, showed the E-domain peptides modulate gene expression following membrane depolarization with KCl . To determine the potential role of 14-3-3 proteins, we examined 14-3-3 isoform expression and distribution. 14-3-3γ localized to the myofilaments in neonatal cardiac myocytes, the cardiac myocytes and myofilament extracts from the adult heart. Thermal shift analysis of recombinant 14-3-3γ protein showed the S/A peptide destabilized 14-3-3γ folding. Also, the S/A peptide significantly inhibited 14-3-3γ's ability to interact with myosin binding protein C (MYPC3) and phospholamban (PLN) in heart lysates from dobutamine injected mice. Conversely, the S/E peptide showed no effect on 14-3-3γ stability, did not inhibit 14-3-3γ's interaction with PLN but did inhibit the interaction with MYPC3. Replacing the glutamic acid with a phosphate group on Ser 18 (pSer), significantly increased 14-3-3γ protein stability. We conclude that the state of Ser 18 phosphorylation within the 14-3-3 binding motif of MGF's E-domain, modulates protein-protein interactions within the 14-3-3γ interactome, which includes proteins involved in the regulation of contractile function.
Christopher Solís, Walter C Thompson, James R Peña, Christopher McDermott-Roe, Paulina Langa, Chad M Warren, Magdalena Chrzanowska, Beata M Wolska, R John Solaro, Pieter Detombe, Paul H Goldspink

1348 related Products with: Mechano-growth factor E-domain modulates cardiac contractile function through 14-3-3 protein interactomes.

100.00 ug3x10 μg100.00 ug100.00 ug1 mlProtein3x10 ug100.00 ug100μg3x10 ug100.00 ug1 mg

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#36467583   2022/11/22 To Up

Hinge region mediates signal transmission of luteinizing hormone and chorionic gonadotropin receptor.

Luteinizing hormone-choriogonadotropin receptor (LHCGR), a class A G protein-coupled receptor (GPCR), plays a pivotal role in the maturation of reproductive organs and embryonic development. Compared with other GPCRs, the subfamily of LHCGR has a large extracellular domain (ECD) to interact with glycoprotein hormones. A unique hinge region connects the ECD and transmembrane domain (TMD) to transfer the activation signal. However, the signal transmission mechanism remains largely unknown. Here, both molecular dynamics simulation and evolutional analysis were applied to explore the effect of the hinge region on signal transmission. The glycoprotein hormone determined specific hinge region conformations, including the position of a long hinge loop and the ECD-TMD interface. With the hormone, the hinge region showed a characteristic rotation and displayed an active-like conformational landscape of the ECD-TMD interface with an extended TMD. The active-like hinge region conformation transduces the hormone binding signal downwards from ECD to TMD. The relationship between the hinge region and the intracelluar G protein-binding pocket was also inferred. The hinge region-mediated signal transmission mechanism offers a deeper understanding of LHCGR and provides insights into the elucidation of GPCR activation.
Xinheng He, Jia Duan, Yujie Ji, Lifen Zhao, Hualiang Jiang, Yi Jiang, H Eric Xu, Xi Cheng

2344 related Products with: Hinge region mediates signal transmission of luteinizing hormone and chorionic gonadotropin receptor.

100ug96/kit1 kit(96 Wells)1 kit(96 Wells)125 RIA tubes100 96T200ug100ul100 μg4100 μg

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#36466292   2022/11/18 To Up

Genome-wide characterization of PEBP gene family in and promotes flowering time in .

Phosphatidylethanolamine-binding proteins (PEBP) family plays important roles in regulating plant flowering time and morphogenesis. However, geneme-wide identification and functional analysis of genes in the rigorous short-day plant () have not been studied. In this study, 10 PfPEBP were identified and divided into three subfamilies based on their phylogenetic relationships: FT-like, TFL1-like and MFT-like. Gene structure analysis showed that all genes contain 4 exons and 3 introns. Motifs DPDxP and GIHR essential for anion-binding activity are highly conserved in PfPEBP. A large number of light-responsive elements were detected in promoter regions of . Gene expression of exhibited a diurnal rhythm. It was highly expressed in leaves under the short-day photoperiod, but higher in flowers and seeds under the long-day photoperiod. Overexpression of in not only promoted early flowering of Col-0 or Ler, but also rescued the late flowering phenotype of mutant. We concluded that promotes early flowering by regulating the expression of flowering-related genes , , and . In conclusion, our results provided valuable information for elucidating the functions of in . and shed light on the promoting effect of on flowering.
Huaxiang Xu, Xi Guo, Youjin Hao, Geng Lu, Dan Li, Junxing Lu, Tao Zhang

1399 related Products with: Genome-wide characterization of PEBP gene family in and promotes flowering time in .

100 µl96 wells2596T1roll (50 m)20 ul2 Pieces/Box7 x 25 ul100 96 wells2ug1roll

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#36466275   2022/11/18 To Up

Functional characterization of the sugarcane ( spp.) ammonium transporter AMT2;1 suggests a role in ammonium root-to-shoot translocation.

AMMONIUM TRANSPORTER/METHYLAMMONIUM PERMEASE/RHESUS (AMT) family members transport ammonium across membranes in all life domains. Plant AMTs can be categorized into AMT1 and AMT2 subfamilies. Functional studies of AMTs, particularly AMT1-type, have been conducted using model plants but little is known about the function of AMTs from crops. Sugarcane ( spp.) is a major bioenergy crop that requires heavy nitrogen fertilization but depends on a low carbon-footprint for competitive sustainability. Here, we identified and functionally characterized sugarcane by complementing ammonium uptake-defective mutants of and . Reporter gene driven by the promoter in revealed preferential expression in the shoot vasculature and root endodermis/pericycle according to nitrogen availability and source. Arabidopsis quadruple mutant plants expressing driven by the CaMV35S promoter or by a sugarcane endogenous promoter produced significantly more biomass than mutant plants when grown in NH and showed more N-ammonium uptake by roots and nitrogen translocation to shoots. In , ScAMT2;1 displayed a K of 90.17 µM and V of 338.99 µmoles h g root DW. Altogether, our results suggest that ScAMT2;1 is a functional high-affinity ammonium transporter that might contribute to ammonium uptake and presumably to root-to-shoot translocation under high NH conditions.
Alessandra Koltun, Rodolfo A Maniero, Marielle Vitti, Nathalia de Setta, Ricardo F H Giehl, Joni E Lima, Antonio Figueira

1991 related Products with: Functional characterization of the sugarcane ( spp.) ammonium transporter AMT2;1 suggests a role in ammonium root-to-shoot translocation.

1 kg 1KG 1KG 1 G 1KG 500 G 500 G5 mg100Tests100 MG 2x5L100ug Lyophilized

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