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#34563047   2021/09/07 To Up

Screening for Copy Number Variations of the 15q13.3 Hotspot in Gene and Expression in Patients with Migraines.

a migraine is a neurological disease. Copy number variation (CNV) is a phenomenon in which parts of the genome are repeated. We investigated the effects of the CNV and gene expression at the location 15q13.3 in the Cholinergic Receptor Nicotinic Alpha 7 Subunit () gene, which we believe to be effective in the migraine clinic.
Mehmet Fatih Özaltun, Sırma Geyik, Şenay Görücü Yılmaz

2961 related Products with: Screening for Copy Number Variations of the 15q13.3 Hotspot in Gene and Expression in Patients with Migraines.

25 MG 5 G500 MG 5 G 1 G 100 G 1 G 1 G300 units 100 G 5 G

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#34562493   2021/09/22 To Up

Varespladib (LY315920) prevents neuromuscular blockage and myotoxicity induced by crotoxin on mouse neuromuscular preparations.

Varespladib (LY315920) is a synthetic phospholipase A (PLA) inhibitor that has been demonstrating antiophidic potential against snake venoms that present PLA neurotoxins. In this study, we evaluate the capacity of Varespladib to inhibit the neuromuscular effects of crotoxin (CTX), the main toxic component of Crotalus durissus terrificus snake venom, and its PLA subunit (CB). We performed a myographic study to compare the neuromuscular effects of CTX or CB and the mixture of these substances plus Varespladib in mice phrenic nerve-diaphragm muscle preparations. CTX (5 μg/mL), CB (20 μg/mL), or toxin-inhibitor mixtures pre-incubated with different concentration ratios of Varespladib (1:0.25; 1:0.5; 1:1; w/w) were added to the preparations and maintained throughout the experimentation period. Myotoxicity was assessed by light microscopic analysis of diaphragm muscle after myographic study. CTX and CB blocked the nerve-evoked twitches, and only CTX induced histological alterations in diaphragm muscle. Pre-incubation with Varespladib abolished the muscle-paralyzing activity of CTX and CB, and also the muscle-damaging activity of CTX. These findings emphasize the clinical potential of Varespladib in mitigating the toxic effects of C. d. terrificus snakebites and as a research tool to advance the knowledge of the mechanism of action of snake toxins.
Fernanda Valadares Maciel, Êmylle Karoline Ramos Pinto, Natália Muradas Valério Souza, Thales Augusto Gonçalves de Abreu, Paula Ladeira Ortolani, Consuelo Latorre Fortes-Dias, Walter Luís Garrido Cavalcante

2087 related Products with: Varespladib (LY315920) prevents neuromuscular blockage and myotoxicity induced by crotoxin on mouse neuromuscular preparations.

100.00 ug100.00 ug2ug100ug0.2 mg100ug50 ul0.2 mg100 ul100ug

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#34562454   2021/09/22 To Up

DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells.

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is known primarily for its function in DNA double-stranded break repair and non-homologous end joining (NHEJ). However, DNA-PKcs also has a critical yet undefined role in immunity impacting both myeloid and lymphoid cell lineages spurring interest in targeting DNA-PKcs for therapeutic strategies in immune-related diseases. To gain insight into the function of DNA-PKcs within immune cells, we performed a quantitative phosphoproteomic screen in T cells to identify phosphorylation targets of DNA-PKcs. Our results indicate that DNA-PKcs phosphorylates the transcription factor Egr1 (early growth response protein 1) at serine 301. Expression of Egr1 is induced early upon T cell activation and dictates T cell response by modulating expression of cytokines and key costimulatory molecules such as IL (interleukin) 2, IL6, IFNγ,and NFκB.Inhibition of DNA-PKcs by treatment with a DNA-PKcs specific inhibitor NU7441 or shRNA knockdown increased proteasomal degradation of Egr1. Mutation of serine 301 to alanine via CRISPR-Cas9 reduced EGR1 protein expression and decreased Egr1-dependent transcription of IL2 in activated T cells. Our findings identify DNA-PKcs as a critical intermediary link between T cell activation and T cell fate, and a novel phosphosite involved in regulating Egr1 activity.
Zachary J Waldrip, Lyle Burdine, David K Harrison, Ana Clara Azevedo-Pouly, Aaron J Storey, Olivia G Moffett, Samuel G Mackintosh, Marie Schluterman Burdine

2296 related Products with: DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells.

10 ug400Tests1 mg196 wells100.00 ug1x10e7 cells5 mg100 assays

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#34562452   2021/09/22 To Up

An atomistic model of the coronavirus replication-transcription complex as a hexamer assembled around nsp15.

The SARS-CoV-2 replication-transcription complex, is an assembly of nonstructural viral proteins that collectively act to reproduce the viral genome and generate mRNA transcripts. While the structures of the individual proteins involved are known, how they assemble into a functioning superstructure is not. Applying molecular modeling tools, including protein-protein docking, to the available structures of nsp7-nsp16 and the nucleocapsid, we have constructed an atomistic model of how these proteins associate. Our principal finding is that the complex is hexameric, centered around nsp15. The nsp15 hexamer is capped on two faces by trimers of nsp14/nsp16/(nsp10), which then recruit six nsp12/nsp7/(nsp8) polymerase subunits to the complex. To this, six subunits of nsp13 are arranged around the superstructure, but not evenly distributed. Polymerase subunits that coordinate dimers of nsp13 are capable of binding the nucleocapsid, which positions the 5'-UTR TRS-L RNA over the polymerase active site, a state distinguishing transcription from replication. Analysis of the viral RNA path through the complex indicates the dsRNA that exits the polymerase passes over the nsp14 exonuclease and nsp15 endonuclease sites before being unwound by a convergence of zinc fingers from nsp10 and nsp14. The template strand is then directed away from the complex, while the nascent strand is directed to the sites responsible for mRNA capping. The model presents a cohesive picture of the multiple functions of the coronavirus replication-transcription complex and addresses fundamental questions related to proofreading, template switching, mRNA capping and the role of the endonuclease.
Jason K Perry, Todd C Appleby, John P Bilello, Joy Y Feng, Uli Schmitz, Elizabeth A Campbell

1240 related Products with: An atomistic model of the coronavirus replication-transcription complex as a hexamer assembled around nsp15.

1 kit(96 Wells)100ug Lyophilized100μl100 μg1 ml400 assays1 ml100.00 ul

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#34562422   2021/09/22 To Up

DNA barcode identification and molecular detection of bluetongue virus in Culicoides biting midges (Diptera: Ceratopogonidae) from western Thailand.

Biting midges of the genus Culicoides Latreille are biological vectors of bluetongue virus (BTV), a member of family Reoviridae, genus Orbivirus. About 30 species of Culicoides have been identified as competent BTV vectors worldwide. Even though high seroprevalence of BTV has been reported among livestock ruminants from western Thailand, the Culicoides species which contribute to BTV transmission remain unclear. In the present study, Culicoides were collected from eight sampling sites, located in two BTV prevalent provinces in western Thailand. Adult Culicoides were identified using wing morphology and cytochrome c oxidase subunit I (COI) mtDNA molecular marker. A total of 9,677 Culicoides specimens belonging to 7 subgenera, 3 species groups, and 23 species were identified. After comparing sequencing results with available data from GenBank, COI sequences of five species were reported for the first time from Thailand. The most abundant potential BTV vector species collected were C. peregrinus, followed by C. orientalis, C. imicola, C. oxystoma, and C. fulvus. Out of 72 Culicoides pools, 9 pools (4 from C. orientalis, 2 from C. imicola, 2 from C. oxystoma, and 1 from C. fulvus) were positive by BTV RT-PCR analyses. These results are new to Culicoides BTV vector knowledge in Thailand and will contribute to further BTV studies in this particular region.
Yuki Fujisawa, Thanyaporn Homat, Arunrat Thepparat, Tanasak Changbunjong, Kripitch Sutummaporn, Sudsaijai Kornmatitsuk, Bunlue Kornmatitsuk

2178 related Products with: DNA barcode identification and molecular detection of bluetongue virus in Culicoides biting midges (Diptera: Ceratopogonidae) from western Thailand.

50 100 1 mg1 mg100100 1050 100 200 2510

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#34562210   2021/09/25 To Up

Family-Based Cohort Association Study of PRKCB1, CBLN1 and KCNMB4 Gene Polymorphisms and Autism in Polish Population.

The aim of the study was to perform family-based association analysis of PRKCB1, CBLN1 and KCNMB4 gene polymorphisms and autism disorder. We comprised 206 Caucasian children with autistic spectrum disorder (ASD) and their biological parents. In transmission/disequilibrium test we observed that T-allele of the rs198198 polymorphism of the PRKCB1 gene was more often transmitted to affected children in the male subgroup (p = 0.010). Additionally, the T carrier state was significantly associated with hypotonia (p = 0.048). In the female subgroup, the T-allele carriers more often showed more mobile/vital behavior (p = 0.046). In conclusion, our study showed that the rs198198 of the PRKCB1 gene may be associated with ASD in men and with some features characteristic for the disorder.
Tomasz Iwanicki, Anna Balcerzyk, Beata Kazek, Ewa Emich-Widera, Wirginia Likus, Joanna Iwanicka, Agnieszka Kapinos-Gorczyca, Maciej Kapinos, Alicja Jarosz, Władysław Grzeszczak, Sylwia Górczyńska-Kosiorz, Paweł Niemiec

2410 related Products with: Family-Based Cohort Association Study of PRKCB1, CBLN1 and KCNMB4 Gene Polymorphisms and Autism in Polish Population.

10 mg500 mg20 ul25 mg50 ug 5 G100ul100.00 ul25 mg 5 G96 wells (1 kit)100ug

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#34562089   2021/09/25 To Up

The Protective Effect of Natural Compounds on Doxorubicin-Induced Cardiotoxicity via Nicotinamide Adenine Dinucleotide Phosphate Oxidase Inhibition.

Doxorubicin (DOX) is widely prescribed for the treatment of several human cancers. Unfortunately, cumulative doses of DOX are the main cause of myocardial dysfunction. Although preclinical and pharmaceutical studies were performed to investigate the potential of natural compounds in minimizing DOX toxicity, a comprehensive review of them is not available. This review can help the researchers for an effective search strategy.
Mozhdeh Yousefian, Hossein Hosseinzadeh, A Wallace Hayes, Farzin Hadizadeh, Gholamreza Karimi

1666 related Products with: The Protective Effect of Natural Compounds on Doxorubicin-Induced Cardiotoxicity via Nicotinamide Adenine Dinucleotide Phosphate Oxidase Inhibition.

1 G 25 G 1 G10000 tests500 Units50 ml 100 G20μg/vial1 kit 5 G100 ug/vial 125 ml

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#34561747   2021/09/25 To Up

Redescription and molecular characterization of the tick Argas japonicus Yamaguti, Clifford & Tipton, 1968 (Ixodida: Argasidae).

Species of the genus Argas are parasites that transmit pathogens, eubacteria, and viruses. Argas japonicus Yamaguti, Clifford & Tipton, 1968 was described based on specimens collected from Japan and Korea. Recently, A. japonicus was reported in different areas of China, suggesting that it may be widely distributed. Here, we have redescribed the female, male, and nymphal stages of A. japonicus and provided scanning electron microscope images based on specimens collected in Neimenggu, China. In addition, we compared four A. japonicus individuals with Argas 16S rDNA and cytochrome c oxidase subunit 1 sequences obtained from GenBank.
Xinxin Hu, Jingze Liu, Rong Bao

1896 related Products with: Redescription and molecular characterization of the tick Argas japonicus Yamaguti, Clifford & Tipton, 1968 (Ixodida: Argasidae).

5 G10 6 ml Ready-to-use 5 G100ul10025 mg2.5 mg50 ug

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#34560609   2021/09/21 To Up

Targeted inhibition of TAK1 abrogates TGFβ1 non-canonical signaling axis, NFκB/Smad7 inhibiting human endometriotic cells proliferation and inducing cell death involving autophagy.

Transforming growth factor (TGFβ) is known to play a major role in establishment and maintenance of endometriosis as reported by our group earlier, the underlying mechanism remains to be explored. We deciphered the involvement of TAK1 in TGFβ1- induced cellular responses and delineated the signaling mechanism in human endometriotic cells. The endometriotic cells showed elevated expression of TGFβ1 signaling-effector molecules. TGFβ1 exposure to endometriotic cells induced the expression of the downstream target molecules indicating that TGFβ1 is implicated in the commencement ofTAK1/NFκB-p65/Smad7 cascade. The silencing of TAK1 in endometriotic cells attenuated the TGFβ1 -induced NFκB transcriptional activation and nuclear translocation of NFκB-p65 subunit. The pharmacological inhibition of NFκB by QNZ or knockdown of TAK1 reduced the expression of Smad7 and Cox2. The knockdown of TAK1 in endometriotic cells showed G1 phase cell-cycle arrest and showed low BrdU-incorporation in the presence of TGFβ1. The inhibition of TAK1 attenuated the TGFβ1 signaling activation indicating that TAK1 is a crucial mediator for TGFβ1 action in endometriotic cells. The exposure of endometriotic cells to TAK1 inhibitor, celastrol caused activation of caspase-3 and -9 that led to PARP cleavage and induced apoptosis. Simultaneously, autophagy occurred in celastrol-treated and TAK1-silenced cells as was evidenced by the formation of autophagosome and the increased expression of autophagic markers. Thus, TAK1 activation appears to protect the growth of endometriotic cells by suppressing the cell death process. Overall, our study provided the evidence that of TAK1 significant in the endometriotic cell regulation and mediates a functional cross-talk between TGFβ1 and NFκB-p65 that promotes the growth and inflammatory response in endometriotic cells.
Jyoti B Kaushal, Vinay Shukla, Pushplata Sankhwar, Rajesh K Jha, Anila Dwivedi

2554 related Products with: Targeted inhibition of TAK1 abrogates TGFβ1 non-canonical signaling axis, NFκB/Smad7 inhibiting human endometriotic cells proliferation and inducing cell death involving autophagy.

5 x 50 ug50 ug100ug Lyophilized100ug Lyophilized1.00 flask100ug Lyophilized1.00 flask0.5 ml1.00 flask1001mg1.00 flask

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