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#34563097   2021/09/25 To Up

Effectiveness of a synthetic human recombinant epidermal growth factor in diabetic patients wound healing: Pilot, double-blind, randomized clinical controlled trial.

To investigate whether the addition of human recombinant epidermal growth factor (h-EGF) to 2% carboxymethyl cellulose gel is more effective in diabetic wound healing than standard treatment, a pilot, double-blind, randomized and controlled clinical trial with therapeutic intervention was performed at a university hospital. The sample consisted of 25 patients (14 in the intervention group that used rh-EGF and 11 in the control group that used 2% carboxymethyl cellulose gel). Data were tabulated in SPSS and analysed by intention to treat, without loss or exclusion of participants. Twenty-five subjects participated with a mean age of 60.6 years, a predominance of males in both groups and 100% prevalence of type-2 diabetes. Within 12 weeks, complete wound healing occurred in three ulcers in the intervention group versus one ulcer in the control group. The percent reduction in the wound area was significantly higher in the intervention group than in the control group (p = 0.049). Concerning the types of tissue, an increase in granulation and epithelial tissue and a reduction in exudate levels were observed in both groups. Decreased slough occurred only in the intervention group. No participant experienced serious or local adverse events during the study period. This study shows that h-EGF is effective, with a statistically significant reduction in wound area, improvement of tissue quality, and safe treatment of chronic wounds. In addition, this study demonstrated that blinding of participants during research using h-EGF is feasible.
Bianca Campos Oliveira, Beatriz Guitton Renaud Baptista de Oliveira, Gabriela Deutsch, Fernanda Soares Pessanha, Selma Rodrigues de Castilho

2056 related Products with: Effectiveness of a synthetic human recombinant epidermal growth factor in diabetic patients wound healing: Pilot, double-blind, randomized clinical controlled trial.

5 x 50 ug5 x 50 ug100 μg50 ug50 ug50 ug20 ug4 Membranes/Box5 x 50 ug100.00 ug8 Sample Kit

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#34563076   2021/08/15 To Up

Association between Change in Regional Phase Angle and Jump Performance: A Pilot Study in Serie A Soccer Players.

This observational longitudinal investigation aimed to investigate whether change in bioelectrical regional phase angle (PhA) is a predictor of change in vertical jump performance in elite soccer players. : Fifteen soccer players (age: 28.7 ± 5.0 years, body weight: 82.4 ± 6.8 kg, height: 186.0 ± 0.1 cm, body mass index: 23.8 ± 1.2 kg/m) competing in the first Italian division (Serie A) were included in this study and tested before the pre-season period and after the first half of the championship. Whole body and lower hemisoma PhA were obtained with a phase-sensitive 50 kHz bioelectrical impedance analyzer and legs lean soft tissue was estimated using specific bioimpedance-based equation developed for athletes. Vertical jump performance was assessed using the countermovement jump (CMJ). Results: The major findings of the study are that changes in lower hemisoma PhA are more strongly related with changes in jump performance (r = 0.617, = 0.001) than changes in whole-body PhA (r = 0.270, = 0.047), even after adjusting for legs lean soft tissue and for body mass index (β = 5.17, = 0.004). These data suggest that changes in lower hemisoma PhA might be used as a tool for evaluating performance related parameters in sports where specific body segments are involved, in preference to the whole-body measured value.
Tindaro Bongiovanni, Athos Trecroci, Alessio Rossi, Fedon Marcello Iaia, Giulio Pasta, Francesco Campa

2118 related Products with: Association between Change in Regional Phase Angle and Jump Performance: A Pilot Study in Serie A Soccer Players.

900 tests100.00 ug1 mg100 μg100 μg100 μg100 μg100.00 ug1 Set1 Set2 mg

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#34563053   2021/09/19 To Up

Oncological Properties of Intravenous Leiomyomatosis: Involvement of Mesenchymal Tumor Stem-Like Cells.

Uterine leiomyoma, also known as fibroids, is the most common benign neoplasm of the female genital tract. Leiomyoma is the most common uterine tumor. The leiomyoma subtypes account for approximately 10% of leiomyomas. Intravenous leiomyomatosis, a uterine leiomyoma subtype, is an intravascular growth of benign smooth muscle cells, occasionally with pelvic or extrapelvic extension. Uterine leiomyosarcoma, a malignant tumor, tends to metastasize hematogenously, and distant metastasis to the lungs and liver is common. Therefore, the oncological properties of this intravenous leiomyomatosis resemble those of the malignant tumor uterine leiomyosarcoma. Cancer stem cells migrate to distant organs via intravascular infiltration, leading to micrometastases. We examined the oncological properties of intravenous leiomyomatosis using molecular pathological techniques on tissue excised from patients with uterine leiomyoma. CD44-positive mesenchymal tumor stem-like cells were detected in both patients with intravenous leiomyomatosis and uterine leiomyosarcoma. The oncological properties of intravenous leiomyomatosis were found to be similar to those of uterine leiomyosarcoma. However, in intravenous leiomyomatosis, cyclin E and Ki-67-positive cells were rare and no pathological findings suspecting malignancy were observed. It is expected that establishing a treatment method targeting cancer stem cells will lead to the treatment of malignant tumors with a low risk of recurrence and metastasis.
Saya Tamura, Takuma Hayashi, Hideki Tokunaga, Nobuo Yaegashi, Kaoru Abiko, Ikuo Konishi

2547 related Products with: Oncological Properties of Intravenous Leiomyomatosis: Involvement of Mesenchymal Tumor Stem-Like Cells.

5 x 10A5 cells/vial24 wells1 x 10^6 cells/vial24 wells1 mg 5 G24 wells10 ug1 vial100ug Lyophilized30ml100|uI x 10 vials

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#34563051   2021/09/17 To Up

HPRep: Quantifying Reproducibility in HiChIP and PLAC-Seq Datasets.

HiChIP and PLAC-Seq are emerging technologies for studying genome-wide long-range chromatin interactions mediated by the protein of interest, enabling more sensitive and cost-efficient interrogation of protein-centric chromatin conformation. However, due to the unbalanced read distribution introduced by protein immunoprecipitation, existing reproducibility measures developed for Hi-C data are not appropriate for the analysis of HiChIP and PLAC-Seq data. Here, we present HPRep, a stratified and weighted correlation metric derived from normalized contact counts, to quantify reproducibility in HiChIP and PLAC-Seq data. We applied HPRep to multiple real datasets and demonstrate that HPRep outperforms existing reproducibility measures developed for Hi-C data. Specifically, we applied HPRep to H3K4me3 PLAC-Seq data from mouse embryonic stem cells and mouse brain tissues as well as H3K27ac HiChIP data from human lymphoblastoid cell line GM12878 and leukemia cell line K562, showing that HPRep can more clearly separate among pseudo-replicates, real replicates, and non-replicates. Furthermore, in an H3K4me3 PLAC-Seq dataset consisting of 11 samples from four human brain cell types, HPRep demonstrated the expected clustering of data that could not be achieved by existing methods developed for Hi-C data, highlighting the need for a reproducibility metric tailored to HiChIP and PLAC-Seq data.
Jonathan D Rosen, Yuchen Yang, Armen Abnousi, Jiawen Chen, Michael Song, Ian R Jones, Yin Shen, Ming Hu, Yun Li

2559 related Products with: HPRep: Quantifying Reproducibility in HiChIP and PLAC-Seq Datasets.

100 ug2 100 μg1 mg96 wells (1 kit)100 μg100ug 100ul20 5mg10 20

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#34563045   2021/09/06 To Up

Regulation of Immunity in Clear Cell Renal Carcinoma: Role of PD-1, PD-L1, and PD-L2.

Regulation of immunity is a unique oncogenic mechanism that differs in different cancers. VHL deficient clear cell renal cell carcinomas (ccRCC) trigger the immune response resulting in cancer progression. This study aimed to investigate PD-1, PD-L1, and PD-L2 expression in ccRCC primary cancers and metastatic tissues associated with the p-VHL content, transcriptional, and growth factors expression.
Liudmila Spirina, Zahar Yurmazov, Evgeny Usynin, Irina Kondakova, Ekaterine Ladutko, Evgeny Choynzonov

2842 related Products with: Regulation of Immunity in Clear Cell Renal Carcinoma: Role of PD-1, PD-L1, and PD-L2.

5 x 50 ug

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#34563043   2021/09/02 To Up

Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers.

Colorectal cancer (CRC) is one of the leading causes of cancer-related death over the world. There is a great need for biomarkers capable of early detection and as targets for treatment. Differential protein expression was investigated with two-dimensional gel electrophoresis (2D-PAGE) followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) in CRC patient tissue from (i) the peripheral part of the tumor, (ii) the central part of the tumor as well as from (iii) a non-involved part of the colorectal tissue. The expression patterns of six identified proteins were further evaluated by one-dimensional Western blot (1D-WB) analysis of the CRC tissue. Proteins that were perturbed in expression level in the peripheral or in the central part of the tumor as compared with the non-involved part included , , or , and . These identified markers may have future diagnostic potential or may be novel treatment targets after further evaluation in larger patient cohorts.
Maja Ludvigsen, Louise Thorlacius-Ussing, Henrik Vorum, Mogens Tornby Stender, Ole Thorlacius-Ussing, Bent Honoré

1957 related Products with: Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers.

100ug

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#34563042   2021/08/31 To Up

Neonatal Diabetes in Patients Affected by Liang-Wang Syndrome Carrying KCNMA1 Variant p.(Gly375Arg) Suggest a Potential Role of Ca and Voltage-Activated K Channel Activity in Human Insulin Secretion.

Liang-Wang syndrome (LIWAS) is a polymalformative syndrome first described in 2019 caused by heterozygous mutation of the KCNMA1 gene encoding the Ca and voltage-activated K channel (BKC). The KCNMA1 variant p.(Gly356Arg) abolishes the function of BKC and blocks the generation of K current. The phenotype of this variant includes developmental delay, and visceral and connective tissue malformations. So far, only three cases of LWAS have been described, one of which also had neonatal diabetes (ND). We present the case of a newborn affected by LIWAS carrying the p.(Gly375Arg) variant who manifested diabetes in the first week of life. The description of our case strongly increases the frequency of ND in LIWAS patients and suggests a role of BK inactivation in human insulin secretion. The knowledge on the role of BKC in insulin secretion is very poor. Analyzing the possible mechanisms that could explain the association of LIWAS with ND, we speculate that BK inactivation might impair insulin secretion through the alteration of ion-dependent membrane activities and mitochondrial functions in β-cells, as well as the impaired intra-islet vessel reactivity.
Chiara Mameli, Roberta Cazzola, Luigina Spaccini, Valeria Calcaterra, Maddalena Macedoni, Paola Azzurra La Verde, Enza D'Auria, Elvira Verduci, Gianluca Lista, Gian Vincenzo Zuccotti

2966 related Products with: Neonatal Diabetes in Patients Affected by Liang-Wang Syndrome Carrying KCNMA1 Variant p.(Gly375Arg) Suggest a Potential Role of Ca and Voltage-Activated K Channel Activity in Human Insulin Secretion.

100 μg 100 UG100 μg100 μg100 μg

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#34563036   2021/09/01 To Up

Immunohistopathological Analysis of Immunoglobulin E-Positive Epidermal Dendritic Cells with House Dust Mite Antigens in Naturally Occurring Skin Lesions of Adult and Elderly Patients with Atopic Dermatitis.

The immunopathogenic role of house dust mite (HDM) allergens in the development of skin lesions in atopic dermatitis (AD) has not yet been precisely clarified. We immunohistopathologically evaluated the localization of immunoglobulin E (IgE)-positive epidermal dendritic cells with HDM antigens in the skin lesions of patients with IgE-allergic AD. Using double-immunofluorescence and single-immunochemical staining methods, we analyzed biopsy specimens from the skin lesions of six patients with IgE-allergic AD and HDM allergy and 11 control subjects with inflammatory skin disorders. Inflammatory dendritic epidermal cells (IDECs; CD11c+ and CD206+ cells) were markedly observed in the central area of the spongiotic epidermis of skin lesions in all AD patients. Furthermore, IgE-positive IDECs with HDM antigens in the central areas of the spongiosis were found in four of the six (66.7%) AD patients. Langerhans cells (LCs; CD207+ cells) with HDM antigens were also observed in the peripheral areas of the spongiosis. Infiltration of CD4+ and CD8+ T cells in association with IgE-positive IDECs and LCs with HDM antigens was seen in the spongiotic epidermis. An IgE-mediated delayed-type hypersensitivity reaction, in combination with IgE-bearing dendritic cells, specific T cells, keratinocytes, and HDM antigens, may lead to spongiotic tissue formation in eczematous dermatitis in AD.
Ryoji Tanei, Yasuko Hasegawa

2755 related Products with: Immunohistopathological Analysis of Immunoglobulin E-Positive Epidermal Dendritic Cells with House Dust Mite Antigens in Naturally Occurring Skin Lesions of Adult and Elderly Patients with Atopic Dermatitis.

1 mg 2 ml100ug1mg100ug1.00 flask96 assays10 rxns

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#34563034   2021/09/18 To Up

Polyelectrolyte Gels: Fundamentals, Fabrication and Applications.

Polyelectrolyte gels are an important class of polymer gels and a versatile platform with charged polymer networks with ionisable groups. They have drawn significant recent attention as a class of smart material and have demonstrated potential for a variety of applications. This review begins with the fundamentals of polyelectrolyte gels, which encompass various classifications (i.e., origin, charge, shape) and crucial aspects (ionic conductivity and stimuli responsiveness). It further centralises recent developments of polyelectrolyte gels, emphasising their synthesis, structure-property relationships and responsive properties. Sequentially, this review demonstrates how polyelectrolyte gels' flourishing properties create attractiveness to a range of applications including tissue engineering, drug delivery, actuators and bioelectronics. Finally, the review outlines the indisputable appeal, further improvements and emerging trends in polyelectrolyte gels.
Nisal Wanasingha, Pramod Dorishetty, Naba K Dutta, Namita Roy Choudhury

2096 related Products with: Polyelectrolyte Gels: Fundamentals, Fabrication and Applications.

1 ml100ug0.2 mg25 mg100 ml1000 tests2.5 mg100ug10 mg50 ug 5 G1000 TESTS/0.65ml

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