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#33249769   2020/11/29 To Up

Inferior meatus augmentation procedure (IMAP) normalizes nasal airflow patterns in empty nose syndrome patients via computational fluid dynamics (CFD) modeling.

Empty nose syndrome (ENS) is a controversial upper airway disorder most commonly associated with tissue loss from the inferior turbinates. The inferior meatus augmentation procedure (IMAP) has been shown to effectively reduce ENS symptoms in a durable manner, but the precise mechanisms that may govern this symptomatic improvement remain unknown.
Jennifer Malik, Sachi Dholakia, Barak M Spector, Angela Yang, Dayoung Kim, Nicole A Borchard, Andrew Thamboo, Kai Zhao, Jayakar V Nayak

1274 related Products with: Inferior meatus augmentation procedure (IMAP) normalizes nasal airflow patterns in empty nose syndrome patients via computational fluid dynamics (CFD) modeling.

400Tests100 μg100 μg50μl5 g100 μg100.00 ug250 MG100 μg100 ul1 mg

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#33249765   2020/11/29 To Up

Time for rethinking the different β-actin transgenic mouse models?

The actin family is crucial for many cellular processes and in mammals muscle and non-muscle forms exist. The latter group contains cytoplasmic-β-actin and cytoplasmic-γ-actin, almost identical in amino acid sequence and with a significant functional overlap. We introduce the properties of the Actb gene and mRNA transcript(s) with main focus on the 3'UTR and its unique features, i.e. the zipcode and two polyadenylation sites creating transcripts of different lengths. Several transgenic mouse models with a modified Actb locus have been created. The different mouse models can be divided into three groups; i.e. 5' or 3' insertion models, mouse models with loxP sequences around exon 2-3 resulting in deletion the start codon, and models with gene edited Actb sequences that produces γ-actin protein instead of β-actin. Whole body knockouts and, with one exception, insertion models lead to embryonic lethality indicating that the Actb gene or transcripts or translated β-actin are essential. Tissue specific ablation at later developmental stages lead to no, or mild phenotypes, suggesting that the Actb gene or β-actin protein is somewhat dispensable. Gene edited Actb mice that produce γ-actin are viable. This assumes that the nucleotide sequence of Actb is important and not the specific amino acid sequence of the protein it encodes. Upregulation of other actin paralogs was frequently observed upon β-actin ablation and can also engage in the phenotype. For a better understanding it will be necessary to analyze in current and future models all relevant actin transcripts and protein levels in a standardized and comprehensive way. This article is protected by copyright. All rights reserved.
Bieke Vanslembrouck, Christophe Ampe, Jolanda van Hengel

2622 related Products with: Time for rethinking the different β-actin transgenic mouse models?

100 100ul100ul5 ml100ug Lyophilized25 µg500 100ul25 µg

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#33249764   2020/11/29 To Up

Type 2 MI induced by a single high dose of isoproterenol in C57BL/6J mice triggers a persistent adaptive immune response against the heart.

Heart failure is the common final pathway of several cardiovascular conditions and a major cause of morbidity and mortality worldwide. Aberrant activation of the adaptive immune system in response to myocardial necrosis has recently been implicated in the development of heart failure. The ß-adrenergic agonist isoproterenol hydrochloride is used for its cardiac effects in a variety of different dosing regimens with high doses causing acute cardiomyocyte necrosis. To assess whether isoproterenol-induced cardiomyocyte necrosis triggers an adaptive immune response against the heart, we treated C57BL/6J mice with a single intraperitoneal injection of isoproterenol. We confirmed tissue damage reminiscent of human type 2 myocardial infarction. This is followed by an adaptive immune response targeting the heart as demonstrated by the activation of T cells, the presence of anti-heart auto-antibodies in the serum as late as 12 weeks after initial challenge and IgG deposition in the myocardium. All of these are hallmark signs of an established autoimmune response. Adoptive transfer of splenocytes from isoproterenol-treated mice induces left ventricular dilation and impairs cardiac function in healthy recipients. In summary, a single administration of a high dose of isoproterenol is a suitable high-throughput model for future studies of the pathological mechanisms of anti-heart autoimmunity and to test potential immunomodulatory therapeutic approaches.
Elvira Forte, Mona Panahi, Nicoleta Baxan, Fu Siong Ng, Joseph J Boyle, Jane Branca, Olivia Bedard, Muneer G Hasham, Lindsay Benson, Sian E Harding, Nadia Rosenthal, Susanne Sattler

2838 related Products with: Type 2 MI induced by a single high dose of isoproterenol in C57BL/6J mice triggers a persistent adaptive immune response against the heart.

100 ul100 ul50 ul 100 UG50 ul2 Pieces/Box

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#33249759   2020/08/16 To Up

The differential relationship of an afterschool physical activity intervention on brain function and cognition in children with obesity and their normal weight peers.

Physical activity (PA) is beneficial for cognitive and brain health during preadolescence. Given that childhood obesity (OB) is a public health concern, investigating this effect in children with OB is an important societal consideration.
Nicole E Logan, Lauren B Raine, Eric S Drollette, Darla M Castelli, Naiman A Khan, Arthur F Kramer, Charles H Hillman

1015 related Products with: The differential relationship of an afterschool physical activity intervention on brain function and cognition in children with obesity and their normal weight peers.

900 tests25 mg2.5 mg

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#33249756   2020/11/29 To Up

Luminal polyethylene-glycol solution delays the onset of preservation injury in the human intestine.

The organ damage incurred during the cold storage (CS) of intestinal grafts has short and long-term consequences. Animal studies suggest that additional luminal preservation (LP) with polyethylene-glycol (PEG) may alleviate this damage. This study aims to validate these findings using human intestines. Ileal segments, perfused intravascularly with IGL-1 solution, were procured from 32 multiorgan donors and divided into two parts: one containing a PEG 3350-based solution introduced luminally (LP group) and another one without luminal treatment (control). Sampling was performed after 4h, 8h, 14h, and 24h of CS. Histology was assessed using the Chiu/Park score. Tight junctions (TJ), several inflammatory markers, and transcription factors were examined by immunofluorescence, ddPCR, and Western blot. Tissue water content (edema) was also measured. Apoptotic activity was assessed with caspase 2,3 and 9 assays. LP significantly lowered mucosal injury at all time points. Redistribution of TJ proteins occurred earlier and more severely in the control group. After 24h of CS, LP intestines showed an emerging unfolding protein response. Increased caspase-3 and -9 activity were found in the control group. The current results indicate that luminal PEG is safe and effective in reducing damage to the intestinal epithelium during CS.
John Mackay Søfteland, Jasmine Bagge, Arvind Manikantan Padma, Anna Casselbrant, Changlian Zhu, Yafeng Wang, Mats Hellström, Michael Olausson, Mihai Oltean

2836 related Products with: Luminal polyethylene-glycol solution delays the onset of preservation injury in the human intestine.

100ul1.00 flask1.00 flask1mg96T200 units10

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#33249742   2020/11/28 To Up

Catechin-Rich Green Tea Extract and the Loss-of-TLR4 Signaling Differentially Alter the Hepatic Metabolome in Mice with Nonalcoholic Steatohepatitis.

Catechin-rich green tea extract (GTE) limits inflammation in nonalcoholic steatohepatitis (NASH) consistent with a Toll-like receptor 4 (TLR4)-dependent mechanism. Our hypothesis was that GTE supplementation during NASH would shift the hepatic metabolome similar to that attributed to the loss-of-TLR4 signaling.
Geoffrey Y Sasaki, Jinhui Li, Morgan J Cichon, Rachel E Kopec, Richard S Bruno

2574 related Products with: Catechin-Rich Green Tea Extract and the Loss-of-TLR4 Signaling Differentially Alter the Hepatic Metabolome in Mice with Nonalcoholic Steatohepatitis.

110 100 G1

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#33249737   2020/11/28 To Up

Structural analysis of an endoprosthesis designed with graded density lattice structures.

The most common causes of conducting a hip revision surgery after total hip replacement are aseptic loosening (aseptic instability) of the endoprosthesis, bone destruction as a result of contact with the endoprosthesis, and a periprosthetic fracture. These are the effects of load transfer to the bone tissue in arthroplasty resulting due to the difference in stiffness of the endoprosthesis and the bone. Titanium alloy is widely used in endoprostheses manufacturing because of its high biocompatibility, good wear properties, and corrosion resistance, but such endoprostheses are stiffer than the femur. These problems have raised interest in searching for the best materials and topology for a femoral implant. Nowadays additive technology is of great interest as it enables to create materials with graded density. These materials consist of multiple lattice structures with variable parameters and topology. By varying the parameters of lattice structures one can adjust the mechanical properties of the material as required. These materials find their application in hip endoprostheses manufacturing, allowing to adjust the parameters of the lattice structures and deliver a product with femur-like mechanical properties. The porous structure also ensures bone tissue ingrowth into the prosthesis. The authors designed and simulated an endoprosthesis made of graded density lattice structures with femur-like mechanical properties. Using a numerical simulation software Ansys Mechanical authors determined the effect of the topology on the structural behavior of the femur and defined the endoprosthesis-femur combined performance under various load cases. This article is protected by copyright. All rights reserved.
V Sh Sufiiarov, E V Borisov, V V Sokolova, M O Chukovenkova, A V Soklakov, D S Mikhaluk, A A Popovich

1612 related Products with: Structural analysis of an endoprosthesis designed with graded density lattice structures.

3 modules 15 ml 100ug Lyophilized1 module1 module4 Sample Kit1 module1 module 1 kit(s) 100ug Lyophilized1 module

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#33249733   2020/11/29 To Up

Renal expression of PLA2R, THSD7A, and IgG4 in patients with membranous nephropathy and correlation with clinical findings.

Membranous nephropathy (MN) is the most common cause of nephrotic syndrome in nondiabetic adults. M-type phospholipase A2 receptor (PLA2R), thrombospondin type-1 domain-containing 7A (THSD7A) are known as target podocyte antigens in membranous nephropathy (MN). Antibodies against these podocyte antigens are used in the initiation of treatment and response monitoring. However, the relationship between renal podocyte antigens and treatment response is not clear yet. We evaluated the relationship between immunohistochemical PLA2R, THSD7A, and IgG4 staining, clinical findings, and treatment response in kidney biopsies.
Bulent Kaya, Saime Paydas, Mustafa Balal, Kivilcim Eren Erdogan, Gulfiliz Gonlusen

2792 related Products with: Renal expression of PLA2R, THSD7A, and IgG4 in patients with membranous nephropathy and correlation with clinical findings.

1,000 tests100ul

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#33249730   2020/11/29 To Up

Post-mortem confirmation of fetal brain abnormalities: challenges highlighted by the MERIDIAN cohort study.

To assess and analyse the concordance between post-mortem findings and in utero MR imaging in the MERIDIAN cohort.
Paul D Griffiths, Deborah Jarvis, Cara Mooney, Gerald Mason, Andrew Dean

2607 related Products with: Post-mortem confirmation of fetal brain abnormalities: challenges highlighted by the MERIDIAN cohort study.

100 U50 ml 500 ml 100.00 ul100 ml1mg 500 ml 100 ml

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#33249703   2020/11/29 To Up

ADFP promotes cell proliferation in lung adenocarcinoma via Akt phosphorylation.

Previously, we identified differentially expressed proteins, including ADFP, between lung adenocarcinoma (LAC) tissue and paired normal bronchioloalveolar epithelium. In this study, we investigated the role of ADFP in LAC. ADFP levels in the serum of patients with lung cancer and benign diseases were measured by enzyme-linked immunosorbent assays (ELISA). shRNA was used to knock-down or overexpress ADFP in A549 and NCI-H1299 cells. The biological function of ADFP and its underlying mechanisms was evaluated in vivo and in vitro. ADFP was highly expressed in the serum of lung cancer patients, especially those with LAC. ADFP promoted cell proliferation and up-regulated the p-Akt/Akt ratio in A549 and NCI-H1299 cells in vitro. Furthermore, in nude mice, ADFP promoted tumour formation with high levels of p-Akt/Akt, Ki67 and proliferating cell nuclear antigen (PCNA). Similar to the effect of ADFP knock-down, MK-2206 (a phosphorylation inhibitor of Akt) reduced A549 and NCI-H1299 cell proliferation. In ADFP-overexpressing A549 and NCI-H1299 cells, proliferation was suppressed by MK-2206 and returned to the control level. ADFP did not regulate invasion, migration or adhesion in LAC cells. Together, these results suggest that ADFP promotes LAC cell proliferation in vitro and in vivo by increasing Akt phosphorylation level.
Xia Meng, Ruiying Sun, Wei Wang, Na Zhang, Shiguang Cao, Boxuan Liu, Ping Fang, Shanshan Deng, Shuanying Yang

1369 related Products with: ADFP promotes cell proliferation in lung adenocarcinoma via Akt phosphorylation.

2 Pieces/Box25 ml.2 Pieces/Box2 Pieces/Box

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